FastQCFastQC Report
Sun 19 Apr 2015
HCNNTADXX l01n02 wt-cdc9deg.34200000008876.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCNNTADXX l01n02 wt-cdc9deg.34200000008876.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2454829
Sequences flagged as poor quality0
Sequence length51
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGTG5600.018.42553744
GGTCGCC1700.018.39269444
ATCTCGG5800.018.32777242
TCTCGGT6100.016.14637243
GGTAGTG5553.8896612E-415.8869214
TCGGTGG7350.014.67631945
GTCGCCG2250.014.59123945
GATCTCG8200.012.578155541
GGATCGA3250.002881128811.6271314
CGTGTGC2200.003263368311.4509614
TGGTCGC2707.2759576E-1111.001554543
CGGGTCG1350.001979701510.49455536
CCCGGGG1600.002282165510.31645516
AGATCTC10400.09.55515540
AGTGTAG9050.08.805860535
GTGGTCG2709.69263E-78.68472942
TAGATCT12150.08.46903839
AGATCGG18801.8189894E-128.4621377
GAGTGTA9450.08.433125534
GTAGATC11550.08.13361838
Produced by FastQC (version 0.11.2)