##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename HCNNTADXX l01n02 rnh201-frbrad27-frb.342000000088e0.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1573759 Sequences flagged as poor quality 0 Sequence length 51 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 2.0 2.0 2.0 2.0 2.0 2.0 2 7.7472884984295565 6.0 2.0 15.0 2.0 15.0 3 2.0 2.0 2.0 2.0 2.0 2.0 4 3.380136348703963 2.0 2.0 2.0 2.0 6.0 5 9.4212303154422 15.0 2.0 15.0 2.0 15.0 6 7.169101495209876 2.0 2.0 15.0 2.0 15.0 7 12.279104996381276 15.0 2.0 15.0 2.0 27.0 8 17.280823811015537 15.0 15.0 22.0 6.0 27.0 9 12.592700661282954 15.0 2.0 22.0 2.0 27.0 10 17.467474371870154 15.0 15.0 27.0 2.0 33.0 11 17.688742685506483 22.0 6.0 27.0 2.0 33.0 12 18.626429459656784 15.0 6.0 27.0 2.0 33.0 13 18.295969713278843 15.0 15.0 27.0 2.0 33.0 14 21.027277365848263 22.0 15.0 27.0 6.0 33.0 15 19.802173013784195 22.0 6.0 27.0 2.0 33.0 16 20.448849537953397 22.0 15.0 33.0 2.0 33.0 17 19.113701653175614 22.0 6.0 33.0 2.0 33.0 18 21.97409832128045 22.0 15.0 33.0 6.0 33.0 19 22.735379432301897 27.0 15.0 33.0 6.0 33.0 20 24.10222467353642 27.0 15.0 33.0 6.0 33.0 21 20.916729944038444 27.0 6.0 33.0 2.0 33.0 22 22.605893278449877 27.0 15.0 33.0 6.0 33.0 23 21.530201892411736 27.0 15.0 33.0 2.0 33.0 24 23.50350593705898 27.0 15.0 33.0 6.0 33.0 25 23.45425760869358 27.0 15.0 33.0 6.0 33.0 26 21.756923391701015 27.0 15.0 33.0 2.0 33.0 27 21.682037084458294 27.0 15.0 33.0 2.0 33.0 28 22.174092094151646 27.0 15.0 33.0 2.0 33.0 29 21.73113735965926 27.0 15.0 33.0 2.0 33.0 30 22.28751416195237 27.0 15.0 33.0 6.0 33.0 31 22.137745995416072 27.0 15.0 33.0 6.0 33.0 32 23.66088581542663 27.0 15.0 33.0 6.0 33.0 33 21.46253651289683 27.0 15.0 33.0 2.0 33.0 34 22.541371963559858 27.0 15.0 33.0 6.0 33.0 35 21.66617442696118 27.0 15.0 27.0 6.0 33.0 36 22.55312916399525 27.0 15.0 27.0 6.0 33.0 37 22.976123408984478 27.0 15.0 33.0 6.0 33.0 38 24.065968804626376 27.0 22.0 33.0 6.0 33.0 39 23.903435024041165 27.0 15.0 33.0 6.0 33.0 40 20.457759415514065 27.0 6.0 27.0 2.0 33.0 41 22.205174998204935 27.0 15.0 27.0 6.0 33.0 42 23.431104762546234 27.0 15.0 33.0 6.0 33.0 43 23.683830243385422 27.0 15.0 33.0 6.0 33.0 44 23.980607577144912 27.0 22.0 33.0 6.0 33.0 45 24.47691355537919 27.0 22.0 33.0 6.0 33.0 46 24.66797521094399 27.0 22.0 33.0 6.0 33.0 47 24.915405726035562 27.0 22.0 33.0 6.0 33.0 48 24.86544953833465 27.0 22.0 33.0 6.0 33.0 49 24.918998398102886 27.0 22.0 33.0 6.0 33.0 50 25.195569334313575 27.0 22.0 33.0 6.0 33.0 51 23.875716040384837 27.0 22.0 33.0 6.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2201 1 0.0 2201 2 -5.694806392483066 2201 3 0.0 2201 4 9.635902598389302 2201 5 3.5671827791759174 2201 6 11.36216408535658 2201 7 11.079642597603431 2201 8 5.517480493651949 2201 9 10.06245065208211 2201 10 4.816442787465032 2201 11 8.187272616403451 2201 12 7.20825299742361 2201 13 7.9275305921331665 2201 14 6.417316788241042 2201 15 3.1173571589850297 2201 16 3.9105348940414046 2201 17 6.704191533936825 2201 18 2.5116663881124204 2201 19 2.4664975242230653 2201 20 1.4980152301129834 2201 21 5.181500813078699 2201 22 4.383162284761138 2201 23 2.6708308313972147 2201 24 2.38687380034343 2201 25 -0.11799891025453135 2201 26 3.7505976204984606 2201 27 4.213823827236279 2201 28 2.8828274820304536 2201 29 4.324862816406402 2201 30 2.190921991420808 2201 31 2.7633791199355286 2201 32 1.4220043354043455 2201 33 2.1106453590417615 2201 34 -0.3280968503966051 2201 35 -3.370112239696656 2201 36 -2.266867803968182 2201 37 -0.6451698777756825 2201 38 -0.32536874851814446 2201 39 0.3827244202000095 2201 40 3.021225269182196 2201 41 0.1711027108677925 2201 42 -1.8526956262206404 2201 43 -2.243276573250938 2201 44 -1.4617123218254555 2201 45 -3.074693030539322 2201 46 -2.0540994300582014 2201 47 -0.7800717171558311 2201 48 -0.735156284584491 2201 49 -0.8158617094264358 2201 50 -1.2387779266320287 2201 51 -1.378463328407399 1101 1 0.0 1101 2 6.419648714787195 1101 3 0.0 1101 4 -1.3765575140556146 1101 5 4.536555171814683 1101 6 7.714455403512261 1101 7 8.200786677821352 1101 8 3.6123901826379097 1101 9 -10.432155030944788 1101 10 -4.5897182422908696 1101 11 0.033222479774764224 1101 12 3.186110973547283 1101 13 6.757143787116018 1101 14 1.8166321945991655 1101 15 2.4733545206407648 1101 16 5.357335860392595 1101 17 6.048133475989474 1101 18 3.2676096719612353 1101 19 0.4571788367181284 1101 20 0.9194782158333048 1101 21 0.15391515615830897 1101 22 1.730889787817933 1101 23 3.830742341541683 1101 24 1.0056418211415838 1101 25 0.5490205234025431 1101 26 0.7039661980071941 1101 27 -0.37962342144929906 1101 28 2.0302182859972895 1101 29 2.4772942758285446 1101 30 2.198890186882622 1101 31 1.6724892385190913 1101 32 0.5289534646549718 1101 33 2.204234809587895 1101 34 2.656859866018994 1101 35 1.5857041642859997 1101 36 0.6579390307320558 1101 37 1.2387097637910855 1101 38 0.8995582997197005 1101 39 0.7197138740071622 1101 40 1.039946391296489 1101 41 -0.6617292837652293 1101 42 1.296974311370029 1101 43 0.37137581484489957 1101 44 -1.3924394187006506 1101 45 -2.3904422356906636 1101 46 -2.257688785960031 1101 47 -1.152828361128197 1101 48 -0.373126830833737 1101 49 -1.6230161694856484 1101 50 -1.1116099335822938 1101 51 -1.4013532306206429 1102 1 0.0 1102 2 6.082373803546087 1102 3 0.0 1102 4 -1.3765575140556146 1102 5 4.33118618677355 1102 6 6.5296192952077945 1102 7 7.840928051091666 1102 8 6.739038079120316 1102 9 -10.432155030944788 1102 10 -3.0444057587259756 1102 11 -15.600601566851374 1102 12 -6.261325191375082 1102 13 -2.716575874378192 1102 14 -1.0987628394991695 1102 15 -1.6711603220613434 1102 16 -18.622717397910172 1102 17 -17.099080511851554 1102 18 -8.04665167072041 1102 19 -5.2836932948541 1102 20 -3.4177420498793794 1102 21 0.06388405192252122 1102 22 1.1405098328220795 1102 23 4.791340147661856 1102 24 2.498367789890974 1102 25 0.6519585362733871 1102 26 4.546021096660738 1102 27 3.9644012808145845 1102 28 4.23738858847852 1102 29 1.170668353980414 1102 30 0.8261751426931205 1102 31 -0.9910231039568949 1102 32 -0.02873815801101287 1102 33 2.365210591135689 1102 34 -0.03159703383572676 1102 35 0.566735369956195 1102 36 2.0624968057069566 1102 37 0.8935742127394342 1102 38 0.7711850031292009 1102 39 -1.5159086009526632 1102 40 2.0260745362728585 1102 41 0.29031796948945754 1102 42 -0.2656643756816237 1102 43 0.8235947590565722 1102 44 1.0966893701247002 1102 45 0.5490672806634507 1102 46 0.9645893358132014 1102 47 0.02085430894454987 1102 48 1.3440846607334898 1102 49 -0.11436663315755169 1102 50 0.36322123584022137 1102 51 0.5063997609335047 1103 1 0.0 1103 2 5.064749826051337 1103 3 0.0 1103 4 -1.3765575140556146 1103 5 4.804921279883345 1103 6 -5.121247756815327 1103 7 1.3911072175218742 1103 8 -0.4342032929216124 1103 9 8.985801336759856 1103 10 6.928640232729162 1103 11 7.046890508479599 1103 12 6.80642687795665 1103 13 -16.411273034468667 1103 14 -7.4014434449357775 1103 15 -18.01129603455192 1103 16 -6.913226441356004 1103 17 -0.494474880674975 1103 18 0.15042572755602635 1103 19 1.1293218086327563 1103 20 1.0801371384399445 1103 21 2.4886479332673233 1103 22 0.6154265325926502 1103 23 1.7236509081868725 1103 24 1.8245625705539688 1103 25 0.3445332632675253 1103 26 2.2235288186528095 1103 27 2.0766721545144122 1103 28 1.0698197391161415 1103 29 3.7606646247243987 1103 30 0.07072515931263013 1103 31 -0.7101533765979369 1103 32 -0.15490051036085006 1103 33 2.0511849403399793 1103 34 0.10681024956340934 1103 35 1.3898778356429844 1103 36 0.7762638407052158 1103 37 1.335581849607184 1103 38 -1.2089895422663837 1103 39 -0.13808227078379431 1103 40 4.383520887682273 1103 41 1.8945989121805233 1103 42 1.1539174612201748 1103 43 1.702028309389366 1103 44 1.4321193290218126 1103 45 1.570591168169635 1103 46 1.0038207037205211 1103 47 -0.24314337243105655 1103 48 -0.4704845696615685 1103 49 -0.19298826770667787 1103 50 0.13264052582230335 1103 51 0.6344348483683433 2103 1 0.0 2103 2 5.212453225547645 2103 3 0.0 2103 4 -1.3765575140556146 2103 5 4.844505044399289 2103 6 -5.121247756815327 2103 7 -10.000790973788959 2103 8 -3.4738262609104265 2103 9 2.965830548888995 2103 10 3.3297454468762346 2103 11 5.7000203716152456 2103 12 5.920139422001675 2103 13 6.181659983308865 2103 14 3.850207180343709 2103 15 5.088489314118895 2103 16 5.480810589320825 2103 17 7.101040573513629 2103 18 2.510318396041697 2103 19 0.7218815313521318 2103 20 2.003225800059756 2103 21 4.713847377600171 2103 22 1.1586626368318917 2103 23 3.194754351609543 2103 24 1.8035391950932897 2103 25 1.116390510450767 2103 26 5.248815597444761 2103 27 4.850288082832073 2103 28 3.026507247115269 2103 29 3.3025901912456277 2103 30 1.0311903657840311 2103 31 4.176477179017798 2103 32 -0.2752223314643807 2103 33 3.77156957987744 2103 34 1.2303233257227149 2103 35 -0.06291531789535298 2103 36 0.8114684079672756 2103 37 -1.9710299074301751 2103 38 0.023333688786973283 2103 39 -0.9782995526454847 2103 40 1.498390144562073 2103 41 1.2592728315991373 2103 42 -0.35812531711681217 2103 43 1.3531268887078518 2103 44 0.6582012183853649 2103 45 1.0145025157965044 2103 46 0.8040997375174754 2103 47 1.1445916563655878 2103 48 0.8054316429520831 2103 49 0.9500897993285307 2103 50 1.2381647138673237 2103 51 1.072194845261624 2102 1 0.0 2102 2 -5.694806392483066 2102 3 0.0 2102 4 -1.3765575140556146 2102 5 -7.361450154015593 2102 6 -5.121247756815327 2102 7 1.489908377328561 2102 8 -4.253166355855205 2102 9 7.72381709114125 2102 10 3.513642379000693 2102 11 5.595264811423078 2102 12 4.170932747277732 2102 13 8.05765270453638 2102 14 4.059238247558518 2102 15 6.521748906568003 2102 16 3.6816325179754337 2102 17 7.863043977453902 2102 18 4.658783249295567 2102 19 2.3042784160368726 2102 20 1.4302055775921474 2102 21 2.692056675813909 2102 22 -1.4786541764481704 2102 23 1.090101845324888 2102 24 -1.7608349857481862 2102 25 1.2810290971167575 2102 26 2.662177251489208 2102 27 2.474515912310302 2102 28 3.6934893902182964 2102 29 1.3562962530097984 2102 30 -0.382379007160786 2102 31 1.0922885915046017 2102 32 -0.10933120662800278 2102 33 2.632731067387514 2102 34 1.6358235790321558 2102 35 1.9038879854751016 2102 36 -0.5648475210544426 2102 37 2.1068998198576523 2102 38 -0.3906842166674238 2102 39 -0.2981926167894713 2102 40 2.069052743357286 2102 41 2.997018292786141 2102 42 2.6836626336552456 2102 43 3.153194504028658 2102 44 0.795087841455107 2102 45 1.7919614609305725 2102 46 1.5711783380726594 2102 47 1.3632742438358463 2102 48 1.1277010500458253 2102 49 1.5268910777313671 2102 50 1.1293235179547025 2102 51 1.2377078015425198 2101 1 0.0 2101 2 -5.694806392483066 2101 3 0.0 2101 4 -1.3765575140556146 2101 5 -7.361450154015593 2101 6 -5.121247756815327 2101 7 -10.000790973788959 2101 8 -4.201284068013205 2101 9 -10.432155030944788 2101 10 -15.338700807733264 2101 11 -15.600601566851374 2101 12 -4.559461441689502 2101 13 -4.068988637191506 2101 14 -2.45673113698874 2101 15 1.6201023681923594 2101 16 3.3221290404239667 2101 17 6.976226343484246 2101 18 2.6130440386627853 2101 19 3.3696566077533916 2101 20 1.5218493486731575 2101 21 3.5311304896208497 2101 22 1.2366177385564292 2101 23 2.1295063278475546 2101 24 1.8816789837568564 2101 25 1.3657374427726836 2101 26 -19.63130989102461 2101 27 -19.551054863945406 2101 28 -20.051427697020348 2101 29 -19.60736632810051 2101 30 -8.323317241770564 2101 31 -8.481811122843045 2101 32 -3.621783804486224 2101 33 -19.37241192708497 2101 34 -9.474452296786069 2101 35 -4.260269648067155 2101 36 -3.785209260294291 2101 37 -1.6894127107701102 2101 38 -0.7959411953914852 2101 39 0.11356345057453865 2101 40 4.299274174460173 2101 41 2.1125592471043717 2101 42 1.2836276995070826 2101 43 -2.1723458091861225 2101 44 -0.5304660435216597 2101 45 0.5880853780734192 2101 46 -0.32656274802923235 2101 47 -0.5260714167690885 2101 48 -0.9309362963593735 2101 49 -0.18702815923263927 2101 50 -0.8626780305864301 2101 51 -0.4724041388295248 2104 1 0.0 2104 2 -5.694806392483066 2104 3 0.0 2104 4 -1.3765575140556146 2104 5 -7.361450154015593 2104 6 -5.121247756815327 2104 7 -10.000790973788959 2104 8 -3.506428777709729 2104 9 1.5585654639621502 2104 10 4.384353962678979 2104 11 4.638532346006606 2104 12 -16.47107638514236 2104 13 -5.727149521056049 2104 14 -5.186456989318762 2104 15 0.8614040881082055 2104 16 3.7835009371119597 2104 17 -17.099080511851554 2104 18 -7.665195800909315 2104 19 -5.165121429862236 2104 20 -5.035169260831914 2104 21 -18.824982497461747 2104 22 -8.786614636933939 2104 23 -19.430926753569608 2104 24 -9.639829175031894 2104 25 -5.190670463029111 2104 26 0.49620330827142567 2104 27 2.3509770276870405 2104 28 3.111176964064395 2104 29 3.2149898129053014 2104 30 2.3877934028381205 2104 31 0.4783534744208495 2104 32 2.2390182108911674 2104 33 4.236835579714668 2104 34 4.204329160681112 2104 35 2.247091850298858 2104 36 2.3087565002054156 2104 37 -1.2691531500193634 2104 38 1.0269067112075945 2104 39 1.714481296389696 2104 40 -18.337484146813345 2104 41 -8.063140680262173 2104 42 -3.9416967867334662 2104 43 -2.987697893590294 2104 44 -0.5974799749392332 2104 45 -0.049072537403585414 2104 46 0.29466284892358274 2104 47 0.17339465833819645 2104 48 -0.7675133722922496 2104 49 0.4562800619490943 2104 50 0.34971589731619446 2104 51 -0.19851655824841075 >>END_MODULE >>Per sequence quality scores fail #Quality Count 5 5.0 6 186.0 7 1842.0 8 7278.0 9 16420.0 10 27911.0 11 39395.0 12 50897.0 13 62490.0 14 75042.0 15 86281.0 16 97115.0 17 106324.0 18 113882.0 19 119032.0 20 119324.0 21 107562.0 22 96186.0 23 92916.0 24 91773.0 25 86906.0 26 76084.0 27 58800.0 28 30053.0 29 8577.0 30 1478.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 NaN NaN NaN NaN 2 38.09873972064584 25.644273592306117 28.759652928962755 7.497333758085293 3 NaN NaN NaN NaN 4 42.16287126706917 21.531970501235868 24.858685522103812 11.44647270959115 5 40.30521741673482 24.629772866851347 23.280769568212047 11.784240148201782 6 15.449792499063687 33.074859888582665 38.08578496823245 13.389562644121197 7 21.3912710340966 33.481861314944155 29.571901492156112 15.554966158803133 8 24.914630659253376 30.14011119936458 28.47491660047657 16.47034154090548 9 20.572078447230087 33.76556813586737 28.754245855236555 16.908107561665993 10 22.775244271496398 27.127941025933076 31.91612372097181 18.18069098159872 11 21.890077484542225 29.17279970241627 27.00404043974392 21.933082373297584 12 26.529596435846976 28.39888677927759 27.177316035803116 17.89420074907232 13 25.71771619770431 28.565847729837664 27.211489933359918 18.504946139098116 14 21.435810692742663 32.95790524470392 27.437301391127868 18.16898267142555 15 20.494348692617876 32.646186149202165 27.89118649130435 18.96827866687561 16 25.532201200312187 28.54672903654253 27.469197539440028 18.45187222370526 17 22.029431532774417 33.673938640509796 26.745939098693 17.55069072802279 18 24.539435223546754 29.552016877208143 27.996975319624838 17.911572579620263 19 21.747675470005255 29.184709984184366 27.044166228755483 22.023448317054896 20 25.32249219861491 29.345471574745563 27.447341047771612 17.884695178867922 21 20.57525060450944 29.964768208863063 30.78078381357448 18.679197373053018 22 22.58472831529558 28.577791954940345 26.447129632924455 22.390350096839622 23 25.606673553361304 28.704459808057432 26.727180129385218 18.961686509196046 24 21.715650236154328 28.763171489408478 31.254785516715078 18.266392757722116 25 25.02555982205662 29.154273303599854 27.423258580252757 18.39690829409077 26 20.874219639844867 29.819129456883747 30.679724332819102 18.626926570452284 27 20.59578124650822 33.35894872140526 27.56210950650489 18.483160525581628 28 25.929987030897315 28.55884360876997 27.429760305894924 18.081409054437792 29 25.27366533855926 29.457816429940593 27.088993769405278 18.17952446209487 30 24.830167770287574 28.909699642702602 27.640127872183733 18.620004714826095 31 20.871493030381398 32.9018610854648 27.564067941787783 18.662577942366017 32 21.636857994140144 33.37480516394187 26.894397426797877 18.093939415120104 33 20.832666513838845 33.090034328588466 27.531787465523628 18.54551169204906 34 25.372595642148056 29.537025093454705 26.756354379996406 18.334024884400833 35 21.60846648260349 33.35752207328426 26.522190838991317 18.51182060512094 36 23.970810452216856 30.377565428025676 27.400086673944568 18.2515374458129 37 20.925512662796493 29.767675047529966 30.942453156294796 18.364359133378745 38 25.095331746853784 29.520275272206213 26.778546306033814 18.605846674906193 39 20.112799990341596 30.10651567361966 31.705362765201023 18.075321570837723 40 21.247123493215412 32.93190884629535 26.962553111676996 18.858414548812245 41 25.460442164270386 29.354367473037485 27.04537352923796 18.13981683345417 42 21.123818831218756 32.77039241713629 27.836727224435254 18.269061527209693 43 20.720728162804676 29.204988962416294 31.91227885117626 18.162004023602766 44 21.175847922599967 29.5108935240641 27.30362617041213 22.0096323829238 45 20.760350799916996 29.548680553875844 31.581794966130673 18.109173680076484 46 20.87081189238261 29.557412940137652 27.77633122759483 21.795443939884912 47 24.777693154146334 29.324699175726206 27.40079931123723 18.496808358890235 48 24.67327401160532 28.997114594546392 27.83142353038593 18.49818786346236 49 20.659507805468703 28.907336293439002 31.48264096117871 18.950514939913592 50 24.693926562759785 29.167608929273293 26.945992586330895 19.192471921636027 51 25.56581246230093 28.984731837408813 27.168934502506055 18.280521197784203 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 236.0 1 369.0 2 502.0 3 596.5 4 691.0 5 792.0 6 893.0 7 1041.5 8 1190.0 9 1439.0 10 1688.0 11 2227.0 12 2766.0 13 3757.0 14 4748.0 15 6579.0 16 8410.0 17 11321.0 18 14232.0 19 18903.5 20 23575.0 21 30007.5 22 36440.0 23 45007.0 24 53574.0 25 63431.0 26 83852.0 27 94416.0 28 103824.0 29 113232.0 30 121135.5 31 129039.0 32 134205.5 33 139372.0 34 139813.5 35 140255.0 36 136776.5 37 133298.0 38 127266.0 39 121234.0 40 114615.5 41 107997.0 42 99842.0 43 91687.0 44 82879.5 45 74072.0 46 69209.0 47 64346.0 48 56277.5 49 48209.0 50 41808.0 51 35407.0 52 29401.0 53 23395.0 54 18628.5 55 13862.0 56 11104.0 57 8346.0 58 6642.0 59 4938.0 60 3969.5 61 3001.0 62 2445.0 63 1889.0 64 1585.5 65 1282.0 66 1084.0 67 886.0 68 712.0 69 538.0 70 462.5 71 387.0 72 298.5 73 210.0 74 164.5 75 91.0 76 63.0 77 42.0 78 21.0 79 17.0 80 13.0 81 10.0 82 7.0 83 5.0 84 3.0 85 2.5 86 2.0 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 100.0 2 49.535538795965586 3 100.0 4 87.45475006020617 5 36.99028885617175 6 62.16580810657795 7 35.31474641288787 8 5.718791759093991E-4 9 37.14412435449138 10 11.855245942993813 11 24.496889294993707 12 11.914848461549704 13 13.63086724206184 14 0.0 15 13.630803699931185 16 12.64170689413055 17 24.556555355680253 18 0.002478143095607396 19 0.0 20 0.0 21 11.914848461549704 22 0.0014614690051017976 23 11.914848461549704 24 0.0 25 0.0 26 11.855627195777753 27 11.855182400863157 28 11.857978254612046 29 11.855182400863157 30 0.0 31 0.0 32 0.0 33 11.85550011151644 34 1.2708426131319978E-4 35 0.0015250111357583975 36 0.0028593958795469954 37 9.531319598489985E-4 38 0.0027958537488903955 39 0.0 40 11.915992219901522 41 0.0 42 0.0 43 0.0028593958795469954 44 0.11246957126218181 45 0.17289813751660832 46 0.1888472123114149 47 0.21648803914703585 48 0.240761133057857 49 0.23955383257538163 50 0.2513726688775092 51 0.2389184112688156 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1573759.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.65918718214552 #Duplication Level Percentage of deduplicated Percentage of total 1 98.88197110380038 96.56732924965546 2 0.7757012061212669 1.5150869857202571 3 0.1756768607796039 0.5146937829134116 4 0.05154942978719062 0.20137101650880468 5 0.03876109961019789 0.18926887411090518 6 0.015112337001800584 0.08855151288111043 7 0.022262662255716315 0.15219074502827196 8 0.006509865167441736 0.050859851273417724 9 0.0043509879236042935 0.03824225496526737 >10 0.026312730771152964 0.5180715653251353 >50 0.0012995428331806118 0.08932102660502907 >100 4.921739484520411E-4 0.07501313501295438 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0075615135481353875 0.0 0.0 5 0.008959440422580586 0.0 0.0 6 0.026814779137085156 0.0 0.0 7 0.7696858286433945 0.0 0.0 8 0.7820765441214316 0.0 0.0 9 0.7952933072980043 0.0 0.0 10 0.8797407989406256 0.0 0.0 11 1.0659192417644634 0.0 0.0 12 1.1679679035989627 0.0 0.0 13 1.2436465812109732 0.0 0.0 14 1.2707155288706848 0.0 0.0 15 1.2961323811333247 0.0 0.0 16 1.3258065561499568 0.0 0.0 17 1.3512869505432534 0.0 0.0 18 1.3849642797912514 0.0 0.0 19 1.4276010494618299 0.0 0.0 20 1.4473626520960325 0.0 0.0 21 1.4724617937053894 0.0 0.0 22 1.4959088399176748 0.0 0.0 23 1.52272361905476 6.35421306565999E-5 0.0 24 1.5496654824531584 6.35421306565999E-5 0.0 25 1.5895063983748465 6.35421306565999E-5 0.0 26 1.6239462331907235 6.35421306565999E-5 0.0 27 1.6507610123278087 6.35421306565999E-5 0.0 28 1.6746528534546903 6.35421306565999E-5 0.0 29 1.697400936229753 6.35421306565999E-5 0.0 30 1.7166542018187028 6.35421306565999E-5 0.0 31 1.7387668632871995 6.35421306565999E-5 0.0 32 1.7633576678513039 6.35421306565999E-5 0.0 33 1.7883297251993475 6.35421306565999E-5 0.0 34 1.8156528413816855 6.35421306565999E-5 0.0 35 1.8476145331019553 6.35421306565999E-5 0.0 36 1.8810376938273268 6.35421306565999E-5 0.0 37 1.911029579497242 6.35421306565999E-5 0.0 38 1.946422546272968 6.35421306565999E-5 0.0 39 1.9813071760034415 6.35421306565999E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGCTCG 160 0.0 77.56483 6 TAGATCG 1280 0.0 63.021423 6 CAGCTCG 100 5.7916113E-6 62.05186 6 TAGTTCG 175 5.5662895E-8 49.641487 6 AGACCGG 190 0.0 47.70153 7 AGTTCGG 205 0.0 47.158585 7 CAGATCG 840 0.0 42.845333 6 CTAGATC 435 0.0 39.94143 5 AGCTCGG 290 0.0 39.58679 7 GACCGGA 180 0.0 39.405727 8 AGATCGG 4670 0.0 37.13297 7 GGAGATC 630 0.0 33.488308 5 TAGACCG 305 1.17379386E-7 32.551796 6 GCAGATC 540 0.0 32.17504 5 GCTCGGA 235 0.0 31.958584 8 GATCGGA 5475 0.0 31.473774 8 GTTCGGA 215 0.0 31.050201 8 ATCGGAA 5485 0.0 30.807844 9 GAGATCG 1460 0.0 30.60092 6 GGTCGCC 870 0.0 29.42731 44 >>END_MODULE