FastQCFastQC Report
Sun 19 Apr 2015
HCNNTADXX l01n02 rnh201-frbrad27-frb.342000000088e0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCNNTADXX l01n02 rnh201-frbrad27-frb.342000000088e0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1573759
Sequences flagged as poor quality0
Sequence length51
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCTCG1600.077.564836
TAGATCG12800.063.0214236
CAGCTCG1005.7916113E-662.051866
TAGTTCG1755.5662895E-849.6414876
AGACCGG1900.047.701537
AGTTCGG2050.047.1585857
CAGATCG8400.042.8453336
CTAGATC4350.039.941435
AGCTCGG2900.039.586797
GACCGGA1800.039.4057278
AGATCGG46700.037.132977
GGAGATC6300.033.4883085
TAGACCG3051.17379386E-732.5517966
GCAGATC5400.032.175045
GCTCGGA2350.031.9585848
GATCGGA54750.031.4737748
GTTCGGA2150.031.0502018
ATCGGAA54850.030.8078449
GAGATCG14600.030.600926
GGTCGCC8700.029.4273144
Produced by FastQC (version 0.11.2)