FastQCFastQC Report
Sun 19 Apr 2015
HCNNTADXX l01n02 rnh201-frb rad27-frb dna2-frb.34200000008918.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCNNTADXX l01n02 rnh201-frb rad27-frb dna2-frb.34200000008918.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3272273
Sequences flagged as poor quality0
Sequence length51
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGTG15200.023.90250244
GGTCCTG2650.001798018223.4064524
TCTCGGT16200.021.94268843
ATCTCGG17800.021.02601242
TCGGTGG19300.019.3924645
GTCCTGT3300.006412044618.796095
GTTAACG5451.8477367E-518.209795
GGTCGCC2950.017.51825744
GGTTAAC4400.001680496616.9164814
GGTTTCC5903.6528916E-516.8209084
GTCGCCG3150.016.40573745
GATCTCG22300.016.357541
AGATCTC22400.014.83562940
TAGATCT22600.013.20618339
GTGGTCG3800.013.18698142
AGTTAAC6750.001922024512.864884
AGTGTAG20450.012.48730935
GTGTAGG27700.012.34597123
GTAGATC24400.012.29749838
GAGTGTA21500.012.04242834
Produced by FastQC (version 0.11.2)