##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename HCNNTADXX l01n02 rnh201-frb rad27-frb dna2-frb.34200000008918.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3272273 Sequences flagged as poor quality 0 Sequence length 51 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 2.0 2.0 2.0 2.0 2.0 2.0 2 7.787434605853485 6.0 2.0 15.0 2.0 15.0 3 2.0 2.0 2.0 2.0 2.0 2.0 4 3.3774651442590518 2.0 2.0 2.0 2.0 6.0 5 9.468098780266805 15.0 2.0 15.0 2.0 15.0 6 7.094322814752926 2.0 2.0 15.0 2.0 15.0 7 12.147089805771095 15.0 2.0 15.0 2.0 27.0 8 17.147227936055458 15.0 15.0 22.0 6.0 27.0 9 12.55141334479122 15.0 2.0 15.0 2.0 27.0 10 17.380610969806003 15.0 15.0 27.0 2.0 33.0 11 17.62097172210265 22.0 6.0 27.0 2.0 33.0 12 18.486108891281383 15.0 6.0 27.0 2.0 33.0 13 18.225450627132883 15.0 6.0 27.0 2.0 33.0 14 20.88342048478229 22.0 15.0 27.0 6.0 33.0 15 19.577730831137867 22.0 6.0 27.0 2.0 33.0 16 20.341677482288304 22.0 15.0 27.0 2.0 33.0 17 19.004640199641045 22.0 6.0 27.0 2.0 33.0 18 21.83375164602709 22.0 15.0 27.0 6.0 33.0 19 22.54743262557861 27.0 15.0 33.0 6.0 33.0 20 23.895313746744236 27.0 15.0 33.0 6.0 33.0 21 20.794547398704204 27.0 6.0 33.0 2.0 33.0 22 22.441956401559406 27.0 15.0 33.0 6.0 33.0 23 21.348682704652088 27.0 15.0 33.0 2.0 33.0 24 23.417112203046628 27.0 15.0 33.0 6.0 33.0 25 23.273755276531023 27.0 15.0 33.0 6.0 33.0 26 21.82572633762525 27.0 15.0 33.0 2.0 33.0 27 21.676542880132555 27.0 15.0 33.0 2.0 33.0 28 22.11590475489056 27.0 15.0 33.0 2.0 33.0 29 21.65486253744721 27.0 15.0 33.0 2.0 33.0 30 22.10977293153719 27.0 15.0 33.0 6.0 33.0 31 22.110018937906464 27.0 15.0 27.0 6.0 33.0 32 23.49652978220338 27.0 15.0 33.0 6.0 33.0 33 21.3896230540667 27.0 15.0 33.0 2.0 33.0 34 22.373082869308277 27.0 15.0 33.0 6.0 33.0 35 21.45597998699986 27.0 15.0 27.0 6.0 33.0 36 22.326249979754135 27.0 15.0 27.0 6.0 33.0 37 22.88767226939806 27.0 15.0 27.0 6.0 33.0 38 23.88801148314948 27.0 15.0 33.0 6.0 33.0 39 23.801276666097237 27.0 15.0 33.0 6.0 33.0 40 20.18847724502204 22.0 6.0 27.0 2.0 33.0 41 21.981631422561627 27.0 15.0 27.0 6.0 33.0 42 23.20855839350812 27.0 15.0 27.0 6.0 33.0 43 23.532196121778348 27.0 15.0 33.0 6.0 33.0 44 23.74752962237564 27.0 15.0 33.0 6.0 33.0 45 24.284100073557433 27.0 22.0 33.0 6.0 33.0 46 24.494577316745882 27.0 22.0 33.0 6.0 33.0 47 24.74561932943859 27.0 22.0 33.0 6.0 33.0 48 24.723971380138515 27.0 22.0 33.0 6.0 33.0 49 24.930094768987797 27.0 22.0 33.0 6.0 33.0 50 25.11410722760601 27.0 22.0 33.0 6.0 33.0 51 23.68226245181866 27.0 15.0 33.0 6.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2201 1 0.0 2201 2 -5.671602993109493 2201 3 0.0 2201 4 9.581881903143586 2201 5 3.56237994197312 2201 6 11.077552924103724 2201 7 10.958500121690093 2201 8 5.407566084145262 2201 9 9.891263571988201 2201 10 4.597662861222204 2201 11 8.16207231389723 2201 12 7.192738645354783 2201 13 7.883828081234892 2201 14 6.479960733472083 2201 15 3.018010443790015 2201 16 3.7401361812663865 2201 17 6.597076438091232 2201 18 2.3348332660039617 2201 19 2.408329573816193 2201 20 1.533517412786928 2201 21 5.255980831697521 2201 22 4.4569644766425505 2201 23 2.5320147450777846 2201 24 2.4566287804083196 2201 25 -0.2754022851556357 2201 26 3.7171402900127397 2201 27 4.288499086716165 2201 28 2.860993843987238 2201 29 4.2504845396214606 2201 30 2.1837496912411645 2201 31 2.803795761380755 2201 32 1.4122873355488963 2201 33 1.9936217657804214 2201 34 -0.496342990254135 2201 35 -3.440451168056672 2201 36 -2.420857016838582 2201 37 -0.6922915684351416 2201 38 -0.39551675124355157 2201 39 0.39758298963951333 2201 40 3.0824810840440087 2201 41 0.1616778296042618 2201 42 -1.9154105156756351 2201 43 -2.385278589861059 2201 44 -1.5866169663828487 2201 45 -3.1733324702279297 2201 46 -2.0771074727913295 2201 47 -0.8464304904836162 2201 48 -0.7561542356860933 2201 49 -0.8419992704040169 2201 50 -1.2679793391198828 2201 51 -1.4061701265012623 1101 1 0.0 1101 2 6.479392368313004 1101 3 0.0 1101 4 -1.3688402718776551 1101 5 4.535840931859109 1101 6 7.723234392989418 1101 7 8.158651599269014 1101 8 3.582714214205218 1101 9 -10.333788011941888 1101 10 -4.503706987170139 1101 11 0.021605492525299752 1101 12 3.11577168473357 1101 13 6.748184761984284 1101 14 1.8486061988647187 1101 15 2.5179503379962185 1101 16 5.4121507931280135 1101 17 5.949295715901666 1101 18 3.1748014454551843 1101 19 0.3553497072074343 1101 20 0.8720839095738491 1101 21 0.08594486803688994 1101 22 1.815422790272752 1101 23 3.885897804909984 1101 24 1.0570757322495083 1101 25 0.6463997170266573 1101 26 0.6654443857667047 1101 27 -0.4280559287375496 1101 28 2.0534433050132286 1101 29 2.518327507718034 1101 30 2.3107474926695275 1101 31 1.6189528797754384 1101 32 0.4241926237045952 1101 33 2.1853879177236273 1101 34 2.7886939899778618 1101 35 1.682549055901024 1101 36 0.6861804450655029 1101 37 1.25511768295312 1101 38 0.8454437136426165 1101 39 0.777955500705211 1101 40 0.9753607857071138 1101 41 -0.6830064856343441 1101 42 1.2807272696593053 1101 43 0.4379630255188154 1101 44 -1.4122962118778801 1101 45 -2.564282238653277 1101 46 -2.4093868263687632 1101 47 -1.2379087229541952 1101 48 -0.3318904799049349 1101 49 -1.7054511950116016 1101 50 -1.2566425515238784 1101 51 -1.4338016130347384 1102 1 0.0 1102 2 6.047956168813723 1102 3 0.0 1102 4 -1.3688402718776551 1102 5 4.293576515549821 1102 6 6.470144506131402 1102 7 7.635215254199743 1102 8 6.672639659736223 1102 9 -10.333788011941888 1102 10 -2.849508813304565 1102 11 -15.412560529894492 1102 12 -6.232050102302242 1102 13 -2.766407017775135 1102 14 -1.119416189069213 1102 15 -1.6998935049175898 1102 16 -18.488143949817484 1102 17 -16.976221292215843 1102 18 -7.886851745200678 1102 19 -5.156012490352129 1102 20 -3.4112906535698926 1102 21 -0.04927298766825672 1102 22 1.0425044598348236 1102 23 4.776437226803083 1102 24 2.423927779842728 1102 25 0.6218974141230156 1102 26 4.565114017498274 1102 27 3.9684777336025263 1102 28 4.297539378115907 1102 29 0.9668894545947957 1102 30 0.7232728854919799 1102 31 -1.0889460365321888 1102 32 -0.14583212108680144 1102 33 2.2629936521184852 1102 34 -0.17521314912642083 1102 35 0.5191651026854736 1102 36 1.9739046056435008 1102 37 0.9976884744648267 1102 38 0.796451571051346 1102 39 -1.5811574474210808 1102 40 1.8966166853411117 1102 41 0.21103258894126142 1102 42 -0.28634351035942274 1102 43 0.7867223427354126 1102 44 1.0559775798034394 1102 45 0.5281074581983347 1102 46 0.9240603865921031 1102 47 -0.029155626929359357 1102 48 1.3211197197316977 1102 49 -0.11821359901885842 1102 50 0.39500634576531723 1102 51 0.46350304123970076 1103 1 0.0 1103 2 5.065677281984957 1103 3 0.0 1103 4 -1.3688402718776551 1103 5 4.81171677045967 1103 6 -5.054186364644909 1103 7 1.4163700528279843 1103 8 -0.5605174784674496 1103 9 8.828350304461978 1103 10 6.971442014176663 1103 11 7.035961063662505 1103 12 6.803054292951106 1103 13 -16.271239281309107 1103 14 -7.259957108034159 1103 15 -17.75603980502659 1103 16 -6.794277017708804 1103 17 -0.520075781730128 1103 18 0.09610809444672341 1103 19 1.1165009009203928 1103 20 1.053630359675914 1103 21 2.5457844325068244 1103 22 0.43361029603833146 1103 23 1.7893789590076814 1103 24 1.8532602530035973 1103 25 0.3703689668797523 1103 26 2.147687935203873 1103 27 2.031738398679426 1103 28 1.0339331840818105 1103 29 3.8027107483352296 1103 30 0.14501349297712096 1103 31 -0.7644216148963778 1103 32 -0.11163461940939357 1103 33 2.083980421432379 1103 34 0.11684747615477775 1103 35 1.3602544840657949 1103 36 0.7960454611711789 1103 37 1.4653832052590552 1103 38 -1.1348215607908543 1103 39 -0.18461410198377948 1103 40 4.370753022612572 1103 41 1.8502974073599212 1103 42 1.1838341796204546 1103 43 1.8224805553840362 1103 44 1.458410054059975 1103 45 1.6309703837250389 1103 46 1.0744239578385937 1103 47 -0.2293497575400245 1103 48 -0.5668277721062438 1103 49 -0.2338328410379873 1103 50 0.13017586740465248 1103 51 0.646353670123549 2103 1 0.0 2103 2 5.093386153326296 2103 3 0.0 2103 4 -1.3688402718776551 2103 5 4.827661343769204 2103 6 -5.054186364644909 2103 7 -9.906119370966781 2103 8 -3.3919792100632584 2103 9 3.012378309573121 2103 10 3.127271484870196 2103 11 5.535092854670992 2103 12 5.795268717039491 2103 13 5.89183863979671 2103 14 3.804395611194966 2103 15 4.888661909088036 2103 16 5.404468141302555 2103 17 6.991442571653014 2103 18 2.259718979869941 2103 19 0.5572028061310306 2103 20 1.974750293904492 2103 21 4.690582712063783 2103 22 1.109724631447989 2103 23 3.00293021802975 2103 24 1.7789610248142829 2103 25 1.0412567070718204 2103 26 5.2048375327937855 2103 27 4.752917145671649 2103 28 2.8639005664946673 2103 29 3.2639071863452074 2103 30 0.7264861136669261 2103 31 4.095656326084555 2103 32 -0.27690336498407575 2103 33 3.7243279395724365 2103 34 1.1572989646879854 2103 35 -0.23352474639220944 2103 36 0.6822120107383718 2103 37 -2.3392798486305466 2103 38 -0.09069155864185774 2103 39 -1.1475626750183388 2103 40 1.2673036865432898 2103 41 0.911712027901924 2103 42 -0.5314385677615405 2103 43 1.2289202317701395 2103 44 0.5581272535994124 2103 45 1.06474861166107 2103 46 0.8041902909852681 2103 47 1.1467182907038804 2103 48 0.8265358241245053 2103 49 0.9545102758698398 2103 50 1.2732339245338977 2103 51 1.0570234797687839 2102 1 0.0 2102 2 -5.671602993109493 2102 3 0.0 2102 4 -1.3688402718776551 2102 5 -7.343725167870307 2102 6 -5.054186364644909 2102 7 1.5496210849135146 2102 8 -4.164773564203623 2102 9 7.62444626764605 2102 10 3.4542113627953235 2102 11 5.4848197898743045 2102 12 3.9764953077610556 2102 13 8.026924145717416 2102 14 3.870870407120986 2102 15 6.549931387813572 2102 16 3.556558422811051 2102 17 7.820427195175199 2102 18 4.675666821669505 2102 19 2.310549909243832 2102 20 1.4528516306287713 2102 21 2.548465920927459 2102 22 -1.6117793015845123 2102 23 0.9176459886716444 2102 24 -2.0394088219699427 2102 25 1.1463651814391014 2102 26 2.7049512735685184 2102 27 2.3948645381162947 2102 28 3.567611060485042 2102 29 1.2986855943839117 2102 30 -0.4125336587390116 2102 31 1.0817778586745739 2102 32 -0.24734472411832087 2102 33 2.5633370499147077 2102 34 1.5925320790261317 2102 35 1.8677210867990723 2102 36 -0.6061677574965287 2102 37 2.253672841539924 2102 38 -0.43298697033963407 2102 39 -0.3292831034901056 2102 40 2.151748676903754 2102 41 3.156446194121841 2102 42 2.7231791607423546 2102 43 3.2755619290360407 2102 44 0.7988687727377659 2102 45 1.772686236953387 2102 46 1.6196637616688996 2102 47 1.4429405328086808 2102 48 1.0903913735173774 2102 49 1.6023493208850077 2102 50 1.1407994913028716 2102 51 1.1575367016936369 2101 1 0.0 2101 2 -5.671602993109493 2101 3 0.0 2101 4 -1.3688402718776551 2101 5 -7.343725167870307 2101 6 -5.054186364644909 2101 7 -9.906119370966781 2101 8 -4.151390009239089 2101 9 -10.333788011941888 2101 10 -15.163254725863606 2101 11 -15.412560529894492 2101 12 -4.372867779556204 2101 13 -3.8359882578227893 2101 14 -2.391136402853199 2101 15 1.56611598036449 2101 16 3.3486369163751455 2101 17 7.1142764453407175 2101 18 2.854103403965688 2101 19 3.5268927542677737 2101 20 1.58065971523434 2101 21 3.6265770853613937 2101 22 1.3968187812164814 2101 23 2.330545223212443 2101 24 1.9973235263806899 2101 25 1.5849994031322545 2101 26 -19.576244829540602 2101 27 -19.445740505467352 2101 28 -19.885868639323842 2101 29 -19.396438630666196 2101 30 -8.098784816581965 2101 31 -8.316535066852769 2101 32 -3.465579172141691 2101 33 -19.150619040142626 2101 34 -9.350701070326892 2101 35 -4.184008806130528 2101 36 -3.694479047181339 2101 37 -1.5144657979073344 2101 38 -0.7322318869416442 2101 39 0.10323574809601155 2101 40 4.355607202016198 2101 41 2.1981432974331163 2101 42 1.3357777591245146 2101 43 -2.2189925669230313 2101 44 -0.3955392288372721 2101 45 0.740383665203435 2101 46 -0.2998380590458325 2101 47 -0.5247654641044619 2101 48 -0.8861666550058231 2101 49 -0.17835269616096028 2101 50 -0.8090508843970348 2101 51 -0.3555861173273698 2104 1 0.0 2104 2 -5.671602993109493 2104 3 0.0 2104 4 -1.3688402718776551 2104 5 -7.343725167870307 2104 6 -5.054186364644909 2104 7 -9.906119370966781 2104 8 -3.3942596961133003 2104 9 1.644925582156322 2104 10 4.365882803273923 2104 11 4.585569545158659 2104 12 -16.278410765981544 2104 13 -5.6771410718262825 2104 14 -5.233323250696156 2104 15 0.9152632508918508 2104 16 3.8204705126431335 2104 17 -16.976221292215843 2104 18 -7.508380266210292 2104 19 -5.11881316123452 2104 20 -5.056202668234377 2104 21 -18.704062862925614 2104 22 -8.643266133868435 2104 23 -19.23485016571236 2104 24 -9.52776827472921 2104 25 -5.135885104516959 2104 26 0.5710693946966998 2104 27 2.4372995314188373 2104 28 3.208447301145945 2104 29 3.2954335996675717 2104 30 2.4220487992742754 2104 31 0.5697198923659919 2104 32 2.4108140424868196 2104 33 4.3369702936005545 2104 34 4.366884699860687 2104 35 2.42829499112802 2104 36 2.5831612988979202 2104 37 -1.4258249892439032 2104 38 1.1443534432635936 2104 39 1.963843089472583 2104 40 -18.09987114316803 2104 41 -7.806302859727973 2104 42 -3.7903257753500625 2104 43 -2.9473769276603328 2104 44 -0.4769312531025989 2104 45 7.183531399306275E-4 2104 46 0.36399396112103233 2104 47 0.2779512384991172 2104 48 -0.697007774670471 2104 49 0.5209900048785734 2104 50 0.3944571460340711 2104 51 -0.12885903596227877 >>END_MODULE >>Per sequence quality scores fail #Quality Count 5 4.0 6 310.0 7 2994.0 8 11783.0 9 28552.0 10 51385.0 11 77531.0 12 105418.0 13 134525.0 14 162822.0 15 190124.0 16 214456.0 17 233970.0 18 248346.0 19 256309.0 20 251723.0 21 227073.0 22 202813.0 23 192403.0 24 186613.0 25 172563.0 26 145895.0 27 107836.0 28 50816.0 29 13895.0 30 2114.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 NaN NaN NaN NaN 2 37.72376831593978 26.093086661108394 29.549097260155822 6.634047762796001 3 NaN NaN NaN NaN 4 43.51743741488838 21.635405465106963 24.117205838445088 10.729951281559574 5 42.56919025934127 24.455079717305864 22.48980684040637 10.485923182946497 6 14.724285941477111 33.29961597734452 38.74713516838631 13.228962912792063 7 22.797602063499436 29.031753510025055 30.641753254078317 17.528891172397188 8 23.77412817979484 29.557495836978877 29.0769109940124 17.591464989213886 9 23.030540578066784 29.180358534083105 29.753729587546957 18.035371300303147 10 25.217218649883456 27.96203646060626 28.250420373154856 18.57032451635543 11 24.83426394704806 29.02329166772167 27.47285827221345 18.669586113016816 12 24.201181173400695 29.432766111010867 27.851888350659987 18.514164364928448 13 24.546752859470775 29.36495934626131 27.55734134583449 18.530946448433422 14 23.51020223557142 30.30080314203613 27.68323425337678 18.50576036901567 15 22.720639831307953 29.94411086527461 28.13211289760678 19.20313640581066 16 23.855100786102927 29.5332063612856 28.05007445560676 18.561618397004708 17 23.48056665191053 30.670316144524833 27.560348622879683 18.28876858068496 18 22.851923974935396 30.446454111382486 28.275121447938133 18.42650046574398 19 23.103115174070137 29.866059463865025 27.996441617187806 19.034383744877033 20 23.41525294497128 30.090154458384127 27.965820700167743 18.528771896476854 21 22.12281566199842 30.319791247380184 28.680201812705597 18.877191277915806 22 23.37731038430285 29.671750548630797 27.692187332396767 19.258751734669584 23 23.36460921343012 29.861548711257928 27.81541910141991 18.95842297389204 24 22.104390434416686 30.158577844819185 29.010262896769312 18.726768823994817 25 23.223168252262724 30.061141024077777 27.91850063763056 18.79719008602894 26 22.565918218699125 30.19668174317584 28.454995051673897 18.782404986451134 27 21.79983826233353 30.69444473170231 28.58554688496426 18.920170120999906 28 23.09855280493581 29.890841601394314 28.264220823586435 18.746384770083445 29 23.02514241670269 30.270141889297253 28.210296195028324 18.49441949897173 30 22.306275272960193 29.97134712517786 28.583290142139774 19.139087459722177 31 22.536719888591204 30.45076006800166 28.08946564055016 18.923054402856973 32 22.642517907277295 30.63561628262679 28.037330626142747 18.684535183953173 33 21.84974784470129 30.64571060224268 28.3457371449057 19.15880440815032 34 23.237755179064262 29.985811034022042 27.873173930808086 18.90325985610561 35 22.62339769791076 30.839194497966993 27.607987788200454 18.929420015921792 36 22.135607112394922 30.345580637876076 28.543980920499123 18.97483132922988 37 22.55225687287245 29.880305563543608 28.516778521438923 19.05065904214502 38 23.04006478821588 30.203013263247968 27.949850253651974 18.807071694884176 39 21.73223322137242 30.238766753262947 29.213332750659866 18.81566727470477 40 22.65320129832726 30.41943021560366 27.866046326992755 19.06132215907632 41 23.0706136502753 30.090509007352995 28.073011067848597 18.76586627452311 42 22.018921409956143 30.357225805189785 28.75598361016444 18.867869174689638 43 22.658579526684107 30.030154107752267 28.84241781729734 18.468848548266283 44 22.536765077054536 30.15420085117475 28.246354238848408 19.062679832922306 45 21.738799018309802 30.34297625275859 29.187099412816526 18.73112531611508 46 22.612912697666683 30.14714144739763 28.32784401665503 18.91210183828066 47 22.852237360580336 30.106806993386503 28.415885128426265 18.625070517606893 48 22.280104807474626 29.97820952978475 28.86950868968906 18.872176973051562 49 22.545215482354642 29.876223019136354 28.807159238025914 18.771402260483086 50 22.818336485495713 30.204170045807206 28.186581868239102 18.790911600457978 51 22.66014454816674 30.196677530397054 28.253949679249867 18.88922824218634 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 103.0 1 177.0 2 251.0 3 332.0 4 413.0 5 473.5 6 534.0 7 737.0 8 940.0 9 1305.5 10 1671.0 11 2547.0 12 3423.0 13 5028.0 14 6633.0 15 9746.0 16 12859.0 17 18171.0 18 23483.0 19 32307.0 20 41131.0 21 54496.5 22 67862.0 23 85502.0 24 103142.0 25 124790.5 26 169423.0 27 192407.0 28 215402.0 29 238397.0 30 258343.0 31 278289.0 32 290413.0 33 302537.0 34 307159.0 35 311781.0 36 307191.0 37 302601.0 38 289449.0 39 276297.0 40 258647.0 41 240997.0 42 220007.0 43 199017.0 44 178675.5 45 158334.0 46 138696.0 47 119058.0 48 101415.5 49 83773.0 50 70136.0 51 56499.0 52 46508.0 53 36517.0 54 29759.5 55 23002.0 56 18784.5 57 14567.0 58 12099.0 59 9631.0 60 7951.5 61 6272.0 62 5353.5 63 4435.0 64 3731.0 65 3027.0 66 2623.5 67 2220.0 68 1867.0 69 1514.0 70 1254.5 71 995.0 72 788.0 73 581.0 74 455.0 75 256.5 76 184.0 77 130.5 78 77.0 79 52.5 80 28.0 81 21.0 82 14.0 83 8.0 84 2.0 85 2.0 86 2.0 87 2.5 88 3.0 89 2.0 90 1.0 91 1.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 100.0 2 48.97785117562013 3 100.0 4 87.41061641250593 5 36.38846758812605 6 62.144692695261064 7 35.524511555117805 8 3.0559797425214826E-4 9 36.6121347454812 10 11.346211028236336 11 23.9628539550337 12 11.930330996221892 13 13.508316696070285 14 0.0 15 13.508255576475433 16 12.616642926797367 17 24.54697392301926 18 0.00271982197084412 19 0.0 20 0.0 21 11.930330996221892 22 0.0011001527073077337 23 11.930330996221892 24 0.0 25 6.111959485042965E-5 26 11.346608305602864 27 11.346211028236336 28 11.348411333650951 29 11.346211028236336 30 3.0559797425214826E-5 31 0.0 32 0.0 33 11.346333267426036 34 3.667175691025779E-4 35 8.556743279060151E-4 36 0.002139185819765038 37 7.334351382051558E-4 38 0.0022308652120406826 39 0.0 40 11.930881072575545 41 6.111959485042965E-5 42 3.0559797425214826E-5 43 0.002505903388867616 44 0.11044310789472638 45 0.17302957302156635 46 0.1904486575539388 47 0.21792191543920694 48 0.24453949899656907 49 0.24493677636309685 50 0.2559688632335994 51 0.24123904087464584 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 3272273.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.86519532705174 #Duplication Level Percentage of deduplicated Percentage of total 1 98.91224178427838 96.80065862454973 2 0.6649113822322933 1.301433645946867 3 0.1578964903653231 0.4635771261317486 4 0.10895377940176086 0.4265113161109533 5 0.05903537889373595 0.28887544433209883 6 0.040690010866682315 0.2389281516796634 7 0.022054609680886794 0.15108650789973105 8 0.014491027816262395 0.11345338141826075 9 0.0085977673449868 0.07572799625343123 >10 0.011127769119707013 0.13974780567752104 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 8.556743279060152E-4 0.0 0.0 5 9.167939227564449E-4 0.0 0.0 6 0.002505903388867616 0.0 0.0 7 0.07401582936387031 0.0 0.0 8 0.07532990065315455 0.0 0.0 9 0.07698012971411615 0.0 0.0 10 0.08993748382240724 0.0 0.0 11 0.12260590726996189 0.0 0.0 12 0.1416141012684455 0.0 0.0 13 0.15609944524799735 0.0 0.0 14 0.1638616337940019 0.0 0.0 15 0.16997359327904488 0.0 0.0 16 0.17862201595038066 0.0 0.0 17 0.1873621180139921 0.0 0.0 18 0.1989137214407233 0.0 0.0 19 0.2131240272434482 0.0 0.0 20 0.22308652120406824 0.0 0.0 21 0.2345464452385238 0.0 0.0 22 0.2460369290704046 0.0 0.0 23 0.2599110771014521 0.0 0.0 24 0.2741825024990274 3.0559797425214826E-5 0.0 25 0.2908681518931947 3.0559797425214826E-5 0.0 26 0.3073093229079603 3.0559797425214826E-5 0.0 27 0.3230476185819459 3.0559797425214826E-5 0.0 28 0.3382052781048525 3.0559797425214826E-5 0.0 29 0.3525683828947035 3.0559797425214826E-5 0.0 30 0.36751212383563353 6.111959485042965E-5 0.0 31 0.3841672134323756 6.111959485042965E-5 0.0 32 0.40097510201624376 9.167939227564448E-5 0.0 33 0.41888314330741966 9.167939227564448E-5 0.0 34 0.44079451806129866 9.167939227564448E-5 0.0 35 0.4659757911396757 9.167939227564448E-5 0.0 36 0.49641334937518966 9.167939227564448E-5 0.0 37 0.5275537829514836 9.167939227564448E-5 0.0 38 0.5615057178908972 9.167939227564448E-5 0.0 39 0.6001944214312193 9.167939227564448E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCGGTG 1520 0.0 23.902502 44 GGTCCTG 265 0.0017980182 23.406452 4 TCTCGGT 1620 0.0 21.942688 43 ATCTCGG 1780 0.0 21.026012 42 TCGGTGG 1930 0.0 19.39246 45 GTCCTGT 330 0.0064120446 18.79609 5 GTTAACG 545 1.8477367E-5 18.20979 5 GGTCGCC 295 0.0 17.518257 44 GGTTAAC 440 0.0016804966 16.916481 4 GGTTTCC 590 3.6528916E-5 16.820908 4 GTCGCCG 315 0.0 16.405737 45 GATCTCG 2230 0.0 16.3575 41 AGATCTC 2240 0.0 14.835629 40 TAGATCT 2260 0.0 13.206183 39 GTGGTCG 380 0.0 13.186981 42 AGTTAAC 675 0.0019220245 12.86488 4 AGTGTAG 2045 0.0 12.487309 35 GTGTAGG 2770 0.0 12.345971 23 GTAGATC 2440 0.0 12.297498 38 GAGTGTA 2150 0.0 12.042428 34 >>END_MODULE