##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename HCNNTADXX l01n02 rnh201-frb.34200000008890.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2836582 Sequences flagged as poor quality 0 Sequence length 51 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 2.0 2.0 2.0 2.0 2.0 2.0 2 7.750699257063607 6.0 2.0 15.0 2.0 15.0 3 2.0 2.0 2.0 2.0 2.0 2.0 4 3.358939032962911 2.0 2.0 2.0 2.0 6.0 5 9.39855537403819 15.0 2.0 15.0 2.0 15.0 6 7.0882421167447305 2.0 2.0 15.0 2.0 15.0 7 12.177672283050516 15.0 2.0 15.0 2.0 27.0 8 17.18951470466921 15.0 15.0 22.0 6.0 27.0 9 12.552023174369717 15.0 2.0 15.0 2.0 27.0 10 17.39993485116947 15.0 6.0 27.0 2.0 33.0 11 17.630024092375965 22.0 6.0 27.0 2.0 33.0 12 18.600646129743474 15.0 6.0 27.0 2.0 33.0 13 18.267306215720186 15.0 15.0 27.0 2.0 33.0 14 21.025562807632568 22.0 15.0 27.0 6.0 33.0 15 19.781757058318778 22.0 6.0 27.0 2.0 33.0 16 20.460193994039304 22.0 15.0 33.0 2.0 33.0 17 19.162986298298446 22.0 6.0 33.0 2.0 33.0 18 21.985960215498793 22.0 15.0 33.0 6.0 33.0 19 22.7288895579257 27.0 15.0 33.0 6.0 33.0 20 24.072321195015693 27.0 15.0 33.0 6.0 33.0 21 21.01732719166941 27.0 6.0 33.0 2.0 33.0 22 22.624954258329215 27.0 15.0 33.0 6.0 33.0 23 21.542605501973853 27.0 15.0 33.0 2.0 33.0 24 23.593111709797213 27.0 15.0 33.0 6.0 33.0 25 23.517859875018598 27.0 15.0 33.0 6.0 33.0 26 21.85407367035397 27.0 15.0 33.0 2.0 33.0 27 21.71585908674595 27.0 15.0 33.0 2.0 33.0 28 22.140338266265527 27.0 15.0 33.0 2.0 33.0 29 21.678474657175432 27.0 15.0 33.0 2.0 33.0 30 22.273049747900817 27.0 15.0 33.0 6.0 33.0 31 22.26296296035158 27.0 15.0 33.0 6.0 33.0 32 23.688144040961976 27.0 15.0 33.0 6.0 33.0 33 21.49497070770385 27.0 15.0 33.0 2.0 33.0 34 22.53944994362934 27.0 15.0 33.0 6.0 33.0 35 21.716121726782443 27.0 15.0 27.0 6.0 33.0 36 22.601746397601058 27.0 15.0 27.0 6.0 33.0 37 23.19660492804368 27.0 15.0 33.0 6.0 33.0 38 24.139971627825318 27.0 22.0 33.0 6.0 33.0 39 24.060937071447256 27.0 15.0 33.0 6.0 33.0 40 20.521008735160837 27.0 6.0 27.0 2.0 33.0 41 22.265483952164963 27.0 15.0 27.0 6.0 33.0 42 23.485258666944937 27.0 15.0 33.0 6.0 33.0 43 23.782354255931963 27.0 15.0 33.0 6.0 33.0 44 23.996762653080363 27.0 22.0 33.0 6.0 33.0 45 24.533214622387085 27.0 22.0 33.0 6.0 33.0 46 24.722514984583558 27.0 22.0 33.0 6.0 33.0 47 24.96512034554263 27.0 22.0 33.0 6.0 33.0 48 24.935444489177467 27.0 22.0 33.0 6.0 33.0 49 25.167649657228313 27.0 22.0 33.0 6.0 33.0 50 25.321576813220982 27.0 22.0 33.0 15.0 33.0 51 23.95159773276429 27.0 22.0 33.0 6.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2201 1 0.0 2201 2 -5.66073350462101 2201 3 0.0 2201 4 9.631997609357656 2201 5 3.4993031234215444 2201 6 11.31218746927632 2201 7 11.054417321629609 2201 8 5.545258333029974 2201 9 10.05495476304267 2201 10 4.743703053998956 2201 11 8.194193717132674 2201 12 7.196549608236406 2201 13 7.882662702413491 2201 14 6.378954554973625 2201 15 3.073738512321164 2201 16 3.9116989744907684 2201 17 6.6723282863860796 2201 18 2.4107031311008846 2201 19 2.4942157554207895 2201 20 1.5148000433145619 2201 21 5.208841879751304 2201 22 4.34582898584609 2201 23 2.5651580385641743 2201 24 2.3694005955187833 2201 25 -0.23564761986898475 2201 26 3.7303611822986262 2201 27 4.211453108266237 2201 28 2.848522798069215 2201 29 4.163055872524971 2201 30 2.1535629956385947 2201 31 2.7505420058362233 2201 32 1.3588977258303636 2201 33 2.037624101370607 2201 34 -0.4338515858405074 2201 35 -3.4255523269444623 2201 36 -2.3531741120075793 2201 37 -0.7014165639525878 2201 38 -0.36757028353084564 2201 39 0.4254767360571847 2201 40 3.090863070776642 2201 41 0.2034972374322379 2201 42 -1.7800222047144025 2201 43 -2.345446067051686 2201 44 -1.5130051800755915 2201 45 -3.05150897892079 2201 46 -2.0153754393358163 2201 47 -0.819065126644027 2201 48 -0.7100940428652365 2201 49 -0.7555831720323063 2201 50 -1.223746281200814 2201 51 -1.3532204698149783 1101 1 0.0 1101 2 6.47724694042282 1101 3 0.0 1101 4 -1.3759996584796652 1101 5 4.509236186510632 1101 6 7.761014033028353 1101 7 8.199773537292343 1101 8 3.7298883252884494 1101 9 -10.389277812305256 1101 10 -4.565624719182718 1101 11 0.05645763635563128 1101 12 3.2490523729499614 1101 13 6.764254835535951 1101 14 1.8840278572010227 1101 15 2.591472484887575 1101 16 5.421065601303798 1101 17 6.019106512875741 1101 18 3.259681672297429 1101 19 0.3934742389575341 1101 20 0.9030140433799261 1101 21 0.14894395286635032 1101 22 1.8595387774767858 1101 23 3.8461051979837606 1101 24 1.1522584845435198 1101 25 0.6985750842183762 1101 26 0.7836698410948308 1101 27 -0.4001103785811502 1101 28 2.1264699865245547 1101 29 2.6081321191402402 1101 30 2.3573902754331613 1101 31 1.716023253649226 1101 32 0.5927966769500763 1101 33 2.255016514580941 1101 34 2.767162496320747 1101 35 1.6949573008386096 1101 36 0.7764295014065254 1101 37 1.3373172485429627 1101 38 0.8643163720188163 1101 39 0.790251904907386 1101 40 1.0247117436332154 1101 41 -0.5781404942650319 1101 42 1.3343823512748436 1101 43 0.4964006184070442 1101 44 -1.3234850326499625 1101 45 -2.40637306380043 1101 46 -2.1821333281001962 1101 47 -1.104152670783698 1101 48 -0.2967106784468889 1101 49 -1.5147101755176777 1101 50 -1.0867355465184048 1101 51 -1.3037729832905853 1102 1 0.0 1102 2 6.075266720274975 1102 3 0.0 1102 4 -1.3759996584796652 1102 5 4.31579039937127 1102 6 6.468772612166958 1102 7 7.804397273519326 1102 8 6.632143100265203 1102 9 -10.389277812305256 1102 10 -3.0180013260876617 1102 11 -15.518559221365205 1102 12 -6.209165523634635 1102 13 -2.62629403767526 1102 14 -1.0845515917795474 1102 15 -1.590743146611782 1102 16 -18.588571395774196 1102 17 -17.05178020324024 1102 18 -8.015623523914462 1102 19 -5.340367225851843 1102 20 -3.4144670169432274 1102 21 0.0217123031516806 1102 22 1.024777431723269 1102 23 4.694820460709813 1102 24 2.448183878826139 1102 25 0.6261965334215169 1102 26 4.539423401625683 1102 27 3.934788481538927 1102 28 4.228289814737689 1102 29 1.0797270838460022 1102 30 0.724428087454541 1102 31 -0.9337517448424961 1102 32 -0.08547263407179528 1102 33 2.3618649467680406 1102 34 -0.08480037830085507 1102 35 0.6541907678432608 1102 36 1.9038502136292124 1102 37 0.9165325469445236 1102 38 0.7284649227173539 1102 39 -1.5276529110892554 1102 40 2.0424178035690304 1102 41 0.3252452468088194 1102 42 -0.262466983099511 1102 43 0.8911323471689379 1102 44 1.083188175664155 1102 45 0.5420566918543024 1102 46 0.9054183972814336 1102 47 -0.02133550128696271 1102 48 1.2613334292466938 1102 49 -0.12768155129852232 1102 50 0.3965598763731215 1102 51 0.5376738809130082 1103 1 0.0 1103 2 5.008574403515745 1103 3 0.0 1103 4 -1.3759996584796652 1103 5 4.80913651171066 1103 6 -5.108394822894326 1103 7 1.3771882199636885 1103 8 -0.5493643517250923 1103 9 8.921202287455735 1103 10 6.93287875736527 1103 11 6.974660148893552 1103 12 6.800294644319408 1103 13 -16.355519271004844 1103 14 -7.435683171718017 1103 15 -17.969805330351804 1103 16 -6.924823084436774 1103 17 -0.5137982849195524 1103 18 0.0744921199010129 1103 19 1.0437975528637011 1103 20 0.9208167110306 1103 21 2.544096590538789 1103 22 0.5485633985900371 1103 23 1.8475755755890013 1103 24 1.710441270937853 1103 25 0.34959925280263704 1103 26 2.1346903127643913 1103 27 2.066266360279702 1103 28 1.0251615397395604 1103 29 3.708165054440066 1103 30 0.18613359514741035 1103 31 -0.7347598773809487 1103 32 -0.18132755426275793 1103 33 2.096806956357753 1103 34 0.21960429008921878 1103 35 1.3547653440169825 1103 36 0.6985330969097987 1103 37 1.3840865450888238 1103 38 -1.0863324794313591 1103 39 -0.19824007727819293 1103 40 4.320830553117133 1103 41 1.812838168458324 1103 42 1.1323937755412778 1103 43 1.6675381781565086 1103 44 1.3347554305188538 1103 45 1.459449084132359 1103 46 0.9185227455014697 1103 47 -0.25096216974413466 1103 48 -0.5539855625018077 1103 49 -0.3016638803120486 1103 50 0.043089000163707425 1103 51 0.5432227885508105 2103 1 0.0 2103 2 5.081845954270506 2103 3 0.0 2103 4 -1.3759996584796652 2103 5 4.8507942012476555 2103 6 -5.108394822894326 2103 7 -9.98400371602301 2103 8 -3.436805906209372 2103 9 2.942946452937134 2103 10 3.308386403262322 2103 11 5.708945667290195 2103 12 5.878697754263115 2103 13 6.127042092565187 2103 14 3.9848676209506664 2103 15 5.105085951789281 2103 16 5.5674861590225255 2103 17 7.153426579586714 2103 18 2.654463348896094 2103 19 0.8073620901984278 2103 20 2.099773210387408 2103 21 4.8592347040977195 2103 22 1.3061830356700561 2103 23 3.1876975918832464 2103 24 1.933234535509719 2103 25 1.149779596244457 2103 26 5.29422883441903 2103 27 4.94671502077458 2103 28 3.0769421006178526 2103 29 3.438988460263719 2103 30 0.9542813334540874 2103 31 4.182797600034803 2103 32 -0.09668205110858707 2103 33 3.8388645710126177 2103 34 1.2923980758721925 2103 35 -0.13603168358081064 2103 36 0.8915290627722001 2103 37 -2.0669291332537583 2103 38 0.11880984048471888 2103 39 -0.9799607769164425 2103 40 1.5313797414807304 2103 41 1.2070851803483578 2103 42 -0.37551095961813274 2103 43 1.3381886795568931 2103 44 0.6621195227201859 2103 45 1.1750121087932506 2103 46 0.92680091166417 2103 47 1.2723412987840206 2103 48 0.9817793888361948 2103 49 1.0782982757882138 2103 50 1.2923015733139884 2103 51 1.0452736519067614 2102 1 0.0 2102 2 -5.66073350462101 2102 3 0.0 2102 4 -1.3759996584796652 2102 5 -7.328086807420588 2102 6 -5.108394822894326 2102 7 1.5162347956640634 2102 8 -4.247917744070042 2102 9 7.639396592740592 2102 10 3.357634007008688 2102 11 5.323784553479761 2102 12 3.8911937720491174 2102 13 7.873266439494969 2102 14 3.808024884909731 2102 15 6.299023842277656 2102 16 3.4547847286759037 2102 17 7.695317904567041 2102 18 4.569464750859108 2102 19 2.225247694777977 2102 20 1.3846175138313015 2102 21 2.476091949234153 2102 22 -1.59037148087031 2102 23 0.9993919478241189 2102 24 -1.9297308415983316 2102 25 1.0869832714791627 2102 26 2.6034559312976775 2102 27 2.4337322246490913 2102 28 3.583661619612485 2102 29 1.3080049486472554 2102 30 -0.46801957170599096 2102 31 0.9598160660681714 2102 32 -0.2691102430196821 2102 33 2.4889643805044557 2102 34 1.591694843112414 2102 35 1.8291056992112757 2102 36 -0.7245241611354807 2102 37 2.0920591180321253 2102 38 -0.59141902543508 2102 39 -0.4316478863381512 2102 40 2.0941806536785847 2102 41 3.0139344255314597 2102 42 2.6156601470358574 2102 43 3.0496702254991135 2102 44 0.6740368278834445 2102 45 1.598917018913287 2102 46 1.5081786888609194 2102 47 1.2475529781673416 2102 48 0.9726223003087213 2102 49 1.397749785273266 2102 50 0.9900267826880516 2102 51 1.0906003907350197 2101 1 0.0 2101 2 -5.66073350462101 2101 3 0.0 2101 4 -1.3759996584796652 2101 5 -7.328086807420588 2101 6 -5.108394822894326 2101 7 -9.98400371602301 2101 8 -4.186633664298858 2101 9 -10.389277812305256 2101 10 -15.26859167804988 2101 11 -15.518559221365205 2101 12 -4.442025866496353 2101 13 -4.04429160632354 2101 14 -2.45237768048759 2101 15 1.4857907226306821 2101 16 3.264215518618194 2101 17 7.07717940798446 2101 18 2.6759725557047425 2101 19 3.3418328463338582 2101 20 1.4385432273531435 2101 21 3.597922484980721 2101 22 1.2901904788712457 2101 23 2.238112274020448 2101 24 1.9734562819811607 2101 25 1.3978685065891696 2101 26 -19.699612919075992 2101 27 -19.601222988156337 2101 28 -20.02027647335407 2101 29 -19.57067210652845 2101 30 -8.296954709573974 2101 31 -8.565407798757102 2101 32 -3.652618462071498 2101 33 -19.385604973393885 2101 34 -9.57691716823164 2101 35 -4.356293804601677 2101 36 -3.755618286223296 2101 37 -1.714973373888629 2101 38 -0.8612767283273968 2101 39 -0.014636176250888866 2101 40 4.262902891954564 2101 41 2.0703887643782934 2101 42 1.2223579583471107 2101 43 -2.1821595981590463 2101 44 -0.42418149374844205 2101 45 0.6743634901703217 2101 46 -0.4787678372669255 2101 47 -0.6327418907020679 2101 48 -0.9962476403597975 2101 49 -0.2816373138776278 2101 50 -0.907567139230995 2101 51 -0.48747999485362215 2104 1 0.0 2104 2 -5.66073350462101 2104 3 0.0 2104 4 -1.3759996584796652 2104 5 -7.328086807420588 2104 6 -5.108394822894326 2104 7 -9.98400371602301 2104 8 -3.4865680922802706 2104 9 1.6093333407396404 2104 10 4.50961550168503 2104 11 4.779076719578608 2104 12 -16.364596761687036 2104 13 -5.621121155005932 2104 14 -5.083262474049906 2104 15 1.0054369630572104 2104 16 3.8941434980997833 2104 17 -17.05178020324024 2104 18 -7.629154054844818 2104 19 -4.965562952700463 2104 20 -4.8470977323536815 2104 21 -18.856843864620725 2104 22 -8.784710627307211 2104 23 -19.378861086574567 2104 24 -9.657244205718875 2104 25 -5.073354624886342 2104 26 0.6137834155757389 2104 27 2.4083781712289465 2104 28 3.131228614052713 2104 29 3.264598567666173 2104 30 2.389177994152174 2104 31 0.6247404953921425 2104 32 2.3335165417538803 2104 33 4.306463502799467 2104 34 4.224709426978407 2104 35 2.3848587032168 2104 36 2.562974684648591 2104 37 -1.2466763875134568 2104 38 1.195007381503789 2104 39 1.9364091869083637 2104 40 -18.367286458209897 2104 41 -8.05484852869248 2104 42 -3.8867940847670432 2104 43 -2.915324383577783 2104 44 -0.49342825031262194 2104 45 0.008083648857720505 2104 46 0.4173558613949453 2104 47 0.30836308220953157 2104 48 -0.6586971942178934 2104 49 0.5052280319766709 2104 50 0.49607173441135544 2104 51 -0.07229726414642101 >>END_MODULE >>Per sequence quality scores fail #Quality Count 5 7.0 6 317.0 7 2842.0 8 11269.0 9 26401.0 10 47075.0 11 69484.0 12 92118.0 13 114957.0 14 136101.0 15 157030.0 16 176036.0 17 192168.0 18 206564.0 19 214275.0 20 216665.0 21 193355.0 22 173106.0 23 166141.0 24 165356.0 25 157455.0 26 138554.0 27 107336.0 28 54197.0 29 15279.0 30 2494.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 NaN NaN NaN NaN 2 38.88084036334403 27.681322857974678 25.953819760739915 7.48401701794138 3 NaN NaN NaN NaN 4 38.447242974030594 22.993098541444326 27.717965136961936 10.841693347563144 5 35.138129816225295 27.41318088957545 26.605389816158283 10.843299478040972 6 13.913778879052053 34.0038814273794 39.49253582512316 12.589803868445387 7 22.223555354672584 30.7675281806851 31.13760940132309 15.871307063319225 8 21.972088090991683 33.05569725657811 28.70247732558406 16.269737326846148 9 22.93277060348189 32.032803778733935 29.11103191817293 15.92339369961124 10 23.20935467904012 29.974835071483362 28.549209259445252 18.26660099003126 11 22.98533589437701 31.23905650703066 28.524860303527532 17.250747295064798 12 22.186414706644246 30.455210026778467 29.563366064347356 17.79500920222993 13 22.306931440190404 29.82899306794798 29.468868988499608 18.395206503362015 14 22.093103601447094 30.782928186105675 28.663934270188555 18.460033942258676 15 21.587431852240403 30.28346531204597 28.859449401180754 19.269653434532874 16 22.061610743990204 29.957724439772242 29.133536434454744 18.847128381782813 17 22.065633787747334 31.54962075309759 27.98002470163817 18.404720757516905 18 21.279950925695793 31.028674271068546 28.898207836024138 18.793166967211526 19 21.553933572165374 30.407300053374097 28.993168538755448 19.045597835705085 20 21.738662940115958 30.965648093374348 28.772868191365525 18.52282077514417 21 20.856990840877458 30.649113444675457 29.42865547657413 19.065240237872956 22 21.58743454611412 30.216013247129688 28.89813959899723 19.298412607758962 23 21.42792424754657 30.6830405556702 28.86570491955084 19.023330277232393 24 20.945031731851927 30.552510027913875 29.48809517933908 19.01436306089512 25 21.67901368618993 30.360060100501236 28.778826066018894 19.182100147289944 26 21.42974613671076 31.110827453552297 28.557509280337207 18.90191712939973 27 20.781573302207622 30.979191417114794 29.125413696779617 19.113821583897966 28 21.39566151666847 30.554855283850134 28.985646430815155 19.063836768666242 29 21.320604550494515 31.074625277048295 28.761724659909337 18.843045512547853 30 20.821397019370497 30.503648405017024 29.171940032052664 19.503014543559818 31 21.026538277405695 30.56181700370375 28.81534889525492 19.59629582363563 32 21.067432564967273 31.064146920483875 28.85211850036417 19.016302014184678 33 20.553021539293837 30.830212551923548 29.142889840250934 19.473876068531677 34 21.23593461690053 30.363917881218683 28.845043351592448 19.55510415028834 35 21.16722444695846 31.163589738346808 28.337824131574337 19.33136168312039 36 20.67211775651446 30.701495541860258 29.24152572697944 19.38486097464584 37 20.922865290561763 30.692913979870667 29.024236072111364 19.359984657456206 38 21.169844353792726 31.279109408541593 28.618926795974048 18.932119441691636 39 20.327140199014167 30.996283555349365 29.55821478102872 19.118361464607755 40 20.97170122354748 30.8771910217304 28.840978742189677 19.31012901253244 41 21.22471426859105 31.129106177157002 28.680946773931986 18.965232780319962 42 20.59919297238719 30.771400227456848 29.459751207615366 19.1696555925406 43 21.042736133522347 30.765249696897477 29.132814783294474 19.059199386285698 44 20.919685804255614 31.054132864291738 29.010829820286617 19.015351511166035 45 20.30932601656657 30.967146252564792 29.686170470163255 19.03735726070538 46 20.941144134621364 30.811961946967685 29.106690652361923 19.14020326604903 47 20.935002445430158 31.332144543298252 29.091324801185113 18.64152821008648 48 20.565920085854085 30.896017159505206 29.606120355491335 18.931942399149378 49 20.884101132035454 30.803665638614692 29.32885585768697 18.983377371662883 50 20.909601923212897 31.293183907233264 29.070388177897193 18.72682599165665 51 20.750017762127467 30.85619109999 29.17881001168943 19.214981126193113 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 31.0 1 54.0 2 77.0 3 102.5 4 128.0 5 202.0 6 276.0 7 461.0 8 646.0 9 1063.0 10 1480.0 11 2339.5 12 3199.0 13 4962.0 14 6725.0 15 10100.0 16 13475.0 17 19324.5 18 25174.0 19 34466.0 20 43758.0 21 57757.0 22 71756.0 23 89762.0 24 107768.0 25 129350.5 26 172816.5 27 194700.0 28 215326.0 29 235952.0 30 251746.0 31 267540.0 32 276102.0 33 284664.0 34 283875.0 35 283086.0 36 273845.0 37 264604.0 38 247834.0 39 231064.0 40 210574.0 41 190084.0 42 168676.0 43 147268.0 44 128068.0 45 108868.0 46 92282.0 47 75696.0 48 62858.0 49 50020.0 50 40673.0 51 31326.0 52 25106.0 53 18886.0 54 15092.0 55 11298.0 56 8904.0 57 6510.0 58 5150.5 59 3791.0 60 3067.0 61 2343.0 62 1840.0 63 1337.0 64 1082.0 65 827.0 66 669.5 67 512.0 68 423.5 69 335.0 70 275.0 71 215.0 72 158.0 73 101.0 74 87.5 75 49.5 76 25.0 77 22.5 78 20.0 79 13.0 80 6.0 81 4.0 82 2.0 83 1.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 100.0 2 49.26333876475279 3 100.0 4 87.6127325069397 5 36.87607127169248 6 62.5249331766189 7 35.65230971641222 8 5.288054426066301E-4 9 36.98482892438858 10 11.89702959406779 11 24.43733338221846 12 11.676411963412304 13 13.57009950708282 14 0.0 15 13.5699937459943 16 12.54030378815067 17 24.216715751562973 18 0.0023619976436429478 19 0.0 20 0.0 21 11.676411963412304 22 0.0015159089354723396 23 11.676411963412304 24 0.0 25 0.0 26 11.897452638421875 27 11.89702959406779 28 11.899955650850213 29 11.89702959406779 30 0.0 31 0.0 32 0.0 33 11.897241116244833 34 2.8202956939020273E-4 35 0.0014454015431247889 36 0.002996564174770904 37 8.813424043443835E-4 38 0.002996564174770904 39 0.0 40 11.677575335386038 41 7.050739234755068E-5 42 0.0 43 0.0029260567824233533 44 0.12589094903655174 45 0.19244992741263958 46 0.2117336992196947 47 0.24021868572810515 48 0.2677870761359975 49 0.268174866793909 50 0.28139500285907476 51 0.2654250784923545 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 2836582.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.4632285673031 #Duplication Level Percentage of deduplicated Percentage of total 1 98.96281417712872 97.4419819198617 2 0.8199228745836137 1.6146450681537312 3 0.10183945539046088 0.3008232471984186 4 0.044617643576550965 0.17572788950449605 5 0.030638115058314725 0.15083638629290871 6 0.008522587501641362 0.05034968886953726 7 0.014630740684610908 0.10084129748964492 8 0.006409965906938025 0.05049167505627672 9 0.004279327465597866 0.037922075852355054 >10 0.006325112703548185 0.07638075172092464 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 3.525369617377534E-5 0.0 0.0 5 1.0576108852132602E-4 0.0 0.0 6 4.2304435408530407E-4 0.0 0.0 7 0.014912313481506968 0.0 0.0 8 0.01505332826620207 0.0 0.0 9 0.0155821337088087 0.0 0.0 10 0.017944131352451646 0.0 0.0 11 0.025911466687724875 0.0 0.0 12 0.031869341341092906 0.0 0.0 13 0.036804858805421455 0.0 0.0 14 0.03909634905671685 0.0 0.0 15 0.04212816692766153 0.0 0.0 16 0.04512473110243243 0.0 0.0 17 0.0488968765930264 0.0 0.0 18 0.05316257383005321 0.0 0.0 19 0.0593319706604639 0.0 0.0 20 0.06384444377070714 0.0 0.0 21 0.0689209760197308 0.0 0.0 22 0.07346870282614781 3.525369617377534E-5 0.0 23 0.07769914636700084 3.525369617377534E-5 0.0 24 0.08344549884332624 3.525369617377534E-5 0.0 25 0.09067250655895018 3.525369617377534E-5 0.0 26 0.09768799209753147 3.525369617377534E-5 0.0 27 0.10339909087768306 3.525369617377534E-5 0.0 28 0.10928645813870355 3.525369617377534E-5 0.0 29 0.11425722929920587 3.525369617377534E-5 0.0 30 0.1193690152444033 3.525369617377534E-5 3.525369617377534E-5 31 0.12515062141690245 3.525369617377534E-5 3.525369617377534E-5 32 0.1306501980200114 3.525369617377534E-5 3.525369617377534E-5 33 0.13586774505373017 3.525369617377534E-5 3.525369617377534E-5 34 0.142953737984659 3.525369617377534E-5 3.525369617377534E-5 35 0.1511325954969749 3.525369617377534E-5 3.525369617377534E-5 36 0.16008703432511381 3.525369617377534E-5 3.525369617377534E-5 37 0.16914723424177408 3.525369617377534E-5 3.525369617377534E-5 38 0.17954707461303782 3.525369617377534E-5 3.525369617377534E-5 39 0.190969272173341 3.525369617377534E-5 3.525369617377534E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTGGGG 545 6.997143E-7 20.792204 4 GTGGGGG 425 0.0012055677 17.775297 5 CTCGGTG 375 0.0 14.599192 44 ATCTCGG 420 0.0 13.778876 42 TACCCGG 145 0.0030479124 12.101903 14 GGTGATA 765 0.0043907915 11.521026 4 TCTCGGT 530 0.0 10.034131 43 CGGGGCA 205 7.041686E-5 9.474688 18 TCGGTGG 585 0.0 9.358623 45 GGATATT 1085 0.00544209 9.283565 4 GATCTCG 610 0.0 8.45907 41 GGGGCAC 315 1.3936977E-5 7.847721 19 GAAGACC 550 0.0028432976 7.507939 8 TTTTTGT 1690 0.003054039 7.450198 6 CGTCGGC 215 0.0038730714 7.2750883 43 GGGCACC 380 2.0045576E-4 6.980958 20 AGTGTTA 605 0.0071888417 6.8253984 8 CGTGTAG 790 2.6921043E-10 6.716013 22 GTGTAGG 830 1.2187229E-10 6.6382093 23 GTGCGGA 410 4.5734158E-4 6.5252376 16 >>END_MODULE