FastQCFastQC Report
Sun 19 Apr 2015
HCNNTADXX l01n02 rnh201-frb.34200000008890.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCNNTADXX l01n02 rnh201-frb.34200000008890.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2836582
Sequences flagged as poor quality0
Sequence length51
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGGGG5456.997143E-720.7922044
GTGGGGG4250.001205567717.7752975
CTCGGTG3750.014.59919244
ATCTCGG4200.013.77887642
TACCCGG1450.003047912412.10190314
GGTGATA7650.004390791511.5210264
TCTCGGT5300.010.03413143
CGGGGCA2057.041686E-59.47468818
TCGGTGG5850.09.35862345
GGATATT10850.005442099.2835654
GATCTCG6100.08.4590741
GGGGCAC3151.3936977E-57.84772119
GAAGACC5500.00284329767.5079398
TTTTTGT16900.0030540397.4501986
CGTCGGC2150.00387307147.275088343
GGGCACC3802.0045576E-46.98095820
AGTGTTA6050.00718884176.82539848
CGTGTAG7902.6921043E-106.71601322
GTGTAGG8301.2187229E-106.638209323
GTGCGGA4104.5734158E-46.525237616