##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename HCNNTADXX l01n02 rad27-frb.342000000088b9.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2812676 Sequences flagged as poor quality 0 Sequence length 51 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 2.0 2.0 2.0 2.0 2.0 2.0 2 7.8324446185767576 6.0 2.0 15.0 2.0 15.0 3 2.0 2.0 2.0 2.0 2.0 2.0 4 3.3416074229665984 2.0 2.0 2.0 2.0 6.0 5 9.508680345692145 15.0 2.0 15.0 2.0 15.0 6 7.128442806779025 2.0 2.0 15.0 2.0 15.0 7 12.134330793877432 15.0 2.0 15.0 2.0 27.0 8 17.30004629043658 15.0 15.0 22.0 6.0 27.0 9 12.70070388484134 15.0 2.0 15.0 2.0 27.0 10 17.623758299925054 15.0 15.0 27.0 2.0 33.0 11 17.879354749711663 22.0 6.0 27.0 2.0 33.0 12 18.734870280117583 15.0 15.0 27.0 2.0 33.0 13 18.41650193623439 15.0 15.0 27.0 2.0 33.0 14 21.197994721041457 22.0 15.0 27.0 6.0 33.0 15 19.955771301067028 22.0 6.0 27.0 2.0 33.0 16 20.657829056741694 22.0 15.0 33.0 2.0 33.0 17 19.27955832808329 27.0 6.0 33.0 2.0 33.0 18 22.131479416754722 22.0 15.0 33.0 6.0 33.0 19 22.889208355317145 27.0 15.0 33.0 6.0 33.0 20 24.265892338826085 27.0 15.0 33.0 6.0 33.0 21 21.147611029496467 27.0 6.0 33.0 2.0 33.0 22 22.79486297035279 27.0 15.0 33.0 6.0 33.0 23 21.649992747120535 27.0 15.0 33.0 2.0 33.0 24 23.73430000469304 27.0 15.0 33.0 6.0 33.0 25 23.69199509648463 27.0 15.0 33.0 6.0 33.0 26 22.10586288644693 27.0 15.0 33.0 2.0 33.0 27 21.954924420729583 27.0 15.0 33.0 2.0 33.0 28 22.39312739896099 27.0 15.0 33.0 2.0 33.0 29 21.940479457996585 27.0 15.0 33.0 2.0 33.0 30 22.48040442624746 27.0 15.0 33.0 6.0 33.0 31 22.485764446384866 27.0 15.0 33.0 6.0 33.0 32 23.891351154558862 27.0 15.0 33.0 6.0 33.0 33 21.75890575380883 27.0 15.0 33.0 2.0 33.0 34 22.76143892862171 27.0 15.0 33.0 6.0 33.0 35 21.88673704329969 27.0 15.0 27.0 6.0 33.0 36 22.790674076928873 27.0 15.0 33.0 6.0 33.0 37 23.343720357410522 27.0 15.0 33.0 6.0 33.0 38 24.319523827131174 27.0 22.0 33.0 6.0 33.0 39 24.22950848231364 27.0 22.0 33.0 6.0 33.0 40 20.58763896019307 27.0 6.0 27.0 2.0 33.0 41 22.39180517059199 27.0 15.0 27.0 6.0 33.0 42 23.615235811021247 27.0 15.0 33.0 6.0 33.0 43 23.9532007952569 27.0 22.0 33.0 6.0 33.0 44 24.1659643698741 27.0 22.0 33.0 6.0 33.0 45 24.704886734199032 27.0 22.0 33.0 6.0 33.0 46 24.903405511335112 27.0 22.0 33.0 6.0 33.0 47 25.148938946398378 27.0 22.0 33.0 15.0 33.0 48 25.118766256760466 27.0 22.0 33.0 6.0 33.0 49 25.346483562273082 27.0 22.0 33.0 15.0 33.0 50 25.516041662815056 27.0 22.0 33.0 15.0 33.0 51 24.1194115497128 27.0 22.0 33.0 6.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2201 1 0.0 2201 2 -5.686349017528648 2201 3 0.0 2201 4 9.643014405692291 2201 5 3.6587446759122653 2201 6 11.571617994272184 2201 7 11.188251244033053 2201 8 5.758087528571696 2201 9 10.25388335730712 2201 10 5.0101566727309255 2201 11 8.379665705351368 2201 12 7.466481700591551 2201 13 8.127209656074232 2201 14 6.461889479910251 2201 15 3.203636203873181 2201 16 4.0760450031811075 2201 17 6.858897662730737 2201 18 2.6149924332546064 2201 19 2.5664491083849086 2201 20 1.6900876095070991 2201 21 5.380640448597056 2201 22 4.4658886945228495 2201 23 2.8300513142277595 2201 24 2.533067636117977 2201 25 0.09989617863413613 2201 26 3.9600226473451166 2201 27 4.4527525561201315 2201 28 3.080736708581309 2201 29 4.30433694311623 2201 30 2.32102480825149 2201 31 2.8857027469371523 2201 32 1.6012626746450387 2201 33 2.191113432293996 2201 34 -0.236943983726551 2201 35 -3.458016247248562 2201 36 -2.2408517217208903 2201 37 -0.5112545522376877 2201 38 -0.25319472147466016 2201 39 0.5035421549989643 2201 40 3.2484788601539805 2201 41 0.3176267893044802 2201 42 -1.580429047409993 2201 43 -2.0419564485977233 2201 44 -1.2877783902430409 2201 45 -2.9718081537806746 2201 46 -1.8858065265374435 2201 47 -0.629737948220626 2201 48 -0.5712116329940038 2201 49 -0.683814219559082 2201 50 -1.1275399348310664 2201 51 -1.209412129031353 1101 1 0.0 1101 2 6.4808676725738 1101 3 0.0 1101 4 -1.3775734865274698 1101 5 4.531231606005443 1101 6 7.881637202700974 1101 7 8.343250170184815 1101 8 3.768622565419097 1101 9 -10.476257783483735 1101 10 -4.635052936985852 1101 11 0.05202699314192216 1101 12 3.2954882684402556 1101 13 6.882308760696585 1101 14 1.9914267919917066 1101 15 2.633611898460316 1101 16 5.486568328804736 1101 17 6.147839660903745 1101 18 3.2824447938898977 1101 19 0.4701768329378595 1101 20 0.9398145808181333 1101 21 0.13008492740954125 1101 22 1.9414697795446259 1101 23 3.9113201677239395 1101 24 1.2101595190494159 1101 25 0.6593059533748473 1101 26 0.8184673309186543 1101 27 -0.4186535080038567 1101 28 2.103088965710022 1101 29 2.6052994615063056 1101 30 2.3097176370129127 1101 31 1.7719992472262227 1101 32 0.46687486382558774 1101 33 2.2211553054295656 1101 34 2.713084131712783 1101 35 1.71673942537263 1101 36 0.8113472920539166 1101 37 1.317769983376131 1101 38 0.8809890128226598 1101 39 0.7917344443860017 1101 40 1.1035363693708113 1101 41 -0.5760783098396409 1101 42 1.3421194219648775 1101 43 0.4830535732996353 1101 44 -1.3528209593439406 1101 45 -2.439258774460807 1101 46 -2.2752613718072787 1101 47 -1.1250884218350485 1101 48 -0.37294844063507426 1101 49 -1.5955457688066197 1101 50 -1.098828202685322 1101 51 -1.3393095550930596 1102 1 0.0 1102 2 6.082837907148602 1102 3 0.0 1102 4 -1.3775734865274698 1102 5 4.344821906358023 1102 6 6.551363335106961 1102 7 7.811081494777659 1102 8 6.711259485899767 1102 9 -10.476257783483735 1102 10 -3.095057766949026 1102 11 -15.645967095301845 1102 12 -6.3121384146803425 1102 13 -2.5828786427651504 1102 14 -1.0744206847372446 1102 15 -1.528629659362391 1102 16 -18.768087739149465 1102 17 -17.215405381711115 1102 18 -8.11908979155324 1102 19 -5.40057115519992 1102 20 -3.407265971535459 1102 21 -0.007914588553862956 1102 22 1.0572737688008367 1102 23 4.67839847279221 1102 24 2.42885283576479 1102 25 0.5848774927674114 1102 26 4.566129684052761 1102 27 3.969863047518025 1102 28 4.285988504902523 1102 29 1.158135676275137 1102 30 0.841667594128424 1102 31 -0.9566099413891394 1102 32 0.004621464544833032 1102 33 2.4870213521582443 1102 34 -0.06335466802480738 1102 35 0.6895964311577778 1102 36 1.9709585701679977 1102 37 0.9768862778137795 1102 38 0.762311781781527 1102 39 -1.506914475027731 1102 40 2.0656363971081255 1102 41 0.35713679990401204 1102 42 -0.25107493549493753 1102 43 0.8468397534792409 1102 44 1.0342169215933055 1102 45 0.5504431578654163 1102 46 0.9618777586218634 1102 47 0.004313280103986017 1102 48 1.2681240351442291 1102 49 -0.08654914967697636 1102 50 0.37756092354609905 1102 51 0.43684877519905285 1103 1 0.0 1103 2 5.0707844125888135 1103 3 0.0 1103 4 -1.3775734865274698 1103 5 4.805126484599501 1103 6 -5.200923706416024 1103 7 1.340993221760348 1103 8 -0.5137657617450806 1103 9 9.03634614947126 1103 10 6.991268499471008 1103 11 7.073499712141732 1103 12 6.857190709161923 1103 13 -16.472901156943383 1103 14 -7.4775957253745915 1103 15 -18.105619599229584 1103 16 -7.009106612054309 1103 17 -0.47395098023295645 1103 18 0.14405226905124024 1103 19 1.075872608515862 1103 20 0.9722533918715719 1103 21 2.5343894813702867 1103 22 0.4993112562881592 1103 23 1.8220384838317685 1103 24 1.7755228470315672 1103 25 0.3140540293349794 1103 26 2.186553923020991 1103 27 2.1848149302948237 1103 28 1.1008283995932082 1103 29 3.769047698918076 1103 30 0.26284444785928684 1103 31 -0.635609450595453 1103 32 -0.15065065589396198 1103 33 2.1771131620254742 1103 34 0.21352395212965902 1103 35 1.3678816323859166 1103 36 0.8045229441708912 1103 37 1.3970574278146621 1103 38 -1.0831807357407932 1103 39 -0.12833018065815693 1103 40 4.427569025725159 1103 41 1.8855530939023666 1103 42 1.2222253639946388 1103 43 1.7358021341865815 1103 44 1.4371940552533857 1103 45 1.5673798515805437 1103 46 0.9914745079696523 1103 47 -0.18324933528327847 1103 48 -0.4628701144522438 1103 49 -0.1745302296173925 1103 50 0.10445847687184795 1103 51 0.613448356422694 2103 1 0.0 2103 2 5.11090607780337 2103 3 0.0 2103 4 -1.3775734865274698 2103 5 4.805181553674682 2103 6 -5.200923706416024 2103 7 -10.054545909822073 2103 8 -3.6272932112906915 2103 9 2.8153267004084412 2103 10 3.08310221073447 2103 11 5.5500388218579175 2103 12 5.726139475528736 2103 13 5.891880784761636 2103 14 3.8094509077875642 2103 15 4.770366594064303 2103 16 5.372663959294542 2103 17 6.835383572923995 2103 18 2.2762222455748287 2103 19 0.6369756723602684 2103 20 1.8139998117309055 2103 21 4.598393831073764 2103 22 1.187384788363019 2103 23 2.869252168532384 2103 24 1.6259163185311678 2103 25 0.8989063502407397 2103 26 5.085395644471113 2103 27 4.6758809457641455 2103 28 2.7653661574784927 2103 29 3.1707292537150806 2103 30 0.6747934465404022 2103 31 3.8729461742899574 2103 32 -0.3235400195925564 2103 33 3.6695135566184867 2103 34 1.1072386102104126 2103 35 -0.2755461260665619 2103 36 0.6150035231205742 2103 37 -2.34043566631593 2103 38 -0.16434901664765533 2103 39 -1.2477577445705492 2103 40 1.2138691084090354 2103 41 0.9156839788014786 2103 42 -0.6860248855942785 2103 43 1.0784917907423406 2103 44 0.4103858512728671 2103 45 0.9321109065619524 2103 46 0.6395234098695326 2103 47 0.9609954264587692 2103 48 0.7030273965019163 2103 49 0.7764029619151316 2103 50 1.0860269959529312 2103 51 0.8419263020816814 2102 1 0.0 2102 2 -5.686349017528648 2102 3 0.0 2102 4 -1.3775734865274698 2102 5 -7.381702075516639 2102 6 -5.200923706416024 2102 7 1.4800615987103498 2102 8 -4.355470705888505 2102 9 7.761175663615241 2102 10 3.5637451728597114 2102 11 5.492189901285187 2102 12 4.058177287458644 2102 13 8.004040822578801 2102 14 3.8891985672146667 2102 15 6.4084281823403835 2102 16 3.561086322283984 2102 17 7.784703833305951 2102 18 4.560646256333573 2102 19 2.2857805669007902 2102 20 1.4052044215955313 2102 21 2.617006858100023 2102 22 -1.5214625188468496 2102 23 1.0547155282913572 2102 24 -1.9044711675473138 2102 25 1.1792530594517174 2102 26 2.6853427797235767 2102 27 2.4836185577627177 2102 28 3.633809718574984 2102 29 1.3629427135145207 2102 30 -0.4077834312845745 2102 31 1.0948629391271716 2102 32 -0.2808469018529678 2102 33 2.600564355483673 2102 34 1.6748038466911126 2102 35 1.9518902231408575 2102 36 -0.6677116259602869 2102 37 2.135032148329099 2102 38 -0.5266192358540778 2102 39 -0.4869896192952048 2102 40 2.047117263363674 2102 41 3.0024632076334754 2102 42 2.5611452729520927 2102 43 3.0511170088679513 2102 44 0.6837384267819147 2102 45 1.67310448527304 2102 46 1.5026168846881305 2102 47 1.25883493537415 2102 48 0.9677264538402461 2102 49 1.4289513684107682 2102 50 0.9771161849258156 2102 51 1.037226787736067 2101 1 0.0 2101 2 -5.686349017528648 2101 3 0.0 2101 4 -1.3775734865274698 2101 5 -7.381702075516639 2101 6 -5.200923706416024 2101 7 -10.054545909822073 2101 8 -4.213486266008651 2101 9 -10.476257783483735 2101 10 -15.399968137720492 2101 11 -15.645967095301845 2101 12 -4.560328600648809 2101 13 -4.070602661469318 2101 14 -2.418691169700221 2101 15 1.6494855940108621 2101 16 3.4379427220943164 2101 17 7.277937013790755 2101 18 3.0032698966787947 2101 19 3.5332005445328676 2101 20 1.6020176713688095 2101 21 3.7795662453366 2101 22 1.3466429733144132 2101 23 2.3898018516675883 2101 24 2.141559751725403 2101 25 1.5866304328012895 2101 26 -19.88101899502045 2101 27 -19.750647653841376 2101 28 -20.178886527158806 2101 29 -19.723337149966707 2101 30 -8.415318112689285 2101 31 -8.655560912455426 2101 32 -3.6016268568029126 2101 33 -19.564077282741582 2101 34 -9.616946148379592 2101 35 -4.306757694424903 2101 36 -3.71957278035136 2101 37 -1.572869315982235 2101 38 -0.7499560167056849 2101 39 0.1588803656490967 2101 40 4.376505064041378 2101 41 2.2672393330229035 2101 42 1.3802582611195753 2101 43 -2.146578995093197 2101 44 -0.3180707841335568 2101 45 0.7387038877266399 2101 46 -0.2633522015220038 2101 47 -0.4589628792247211 2101 48 -0.8646584751942648 2101 49 -0.08978305040755785 2101 50 -0.6989683644318099 2101 51 -0.3089572688292428 2104 1 0.0 2104 2 -5.686349017528648 2104 3 0.0 2104 4 -1.3775734865274698 2104 5 -7.381702075516639 2104 6 -5.200923706416024 2104 7 -10.054545909822073 2104 8 -3.527953634957642 2104 9 1.5620414796491389 2104 10 4.481806285859257 2104 11 4.744513056825561 2104 12 -16.531010425851935 2104 13 -5.779057562933399 2104 14 -5.181258167092125 2104 15 0.9687207858429154 2104 16 3.8428880155450926 2104 17 -17.215405381711115 2104 18 -7.7625381032296765 2104 19 -5.167884178432644 2104 20 -5.016111515356613 2104 21 -19.032167203333422 2104 22 -8.976508741987058 2104 23 -19.555577987067004 2104 24 -9.81060774067304 2104 25 -5.322923496605107 2104 26 0.5791069854882238 2104 27 2.4023711243853896 2104 28 3.2090680723182565 2104 29 3.3528454029213357 2104 30 2.41305361018134 2104 31 0.6222691968594916 2104 32 2.283905431126925 2104 33 4.217596118732153 2104 34 4.208594259387002 2104 35 2.314212355682834 2104 36 2.4263037985191467 2104 37 -1.4021863027977837 2104 38 1.133998931818688 2104 39 1.9158350545175793 2104 40 -18.48271208817216 2104 41 -8.169624892729075 2104 42 -3.9882194515319647 2104 43 -3.0067688168848363 2104 44 -0.6068651211809701 2104 45 -0.05067536076610324 2104 46 0.32892753871753655 2104 47 0.1728949426268116 2104 48 -0.6671892222108013 2104 49 0.42486808774170726 2104 50 0.3801739206515009 2104 51 -0.07177126848585047 >>END_MODULE >>Per sequence quality scores fail #Quality Count 5 3.0 6 260.0 7 2461.0 8 10015.0 9 23967.0 10 43816.0 11 64574.0 12 86093.0 13 107976.0 14 128891.0 15 151246.0 16 170905.0 17 188736.0 18 203791.0 19 213997.0 20 217649.0 21 195832.0 22 175550.0 23 167874.0 24 168205.0 25 162279.0 26 141721.0 27 110949.0 28 56669.0 29 16315.0 30 2902.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 NaN NaN NaN NaN 2 39.56002231067968 25.611066651423346 27.81857675878483 7.010334279112146 3 NaN NaN NaN NaN 4 43.663154702367585 21.610099060138865 24.121741615464696 10.605004622028853 5 41.90338252962471 25.191778199037273 22.266089941763923 10.638749329574097 6 16.255197729597175 33.8477494574405 36.63739755651542 13.259655256446912 7 22.21954295266943 30.649451209755473 31.564754339577462 15.566251497997635 8 21.484088211065455 31.407353102735737 30.4785891519887 16.629969534210108 9 22.24511337265992 30.2661054330255 30.499002865957138 16.989778328357442 10 23.57130689260053 28.813116013415897 29.318984261876714 18.296592832106864 11 23.433865052022874 29.68657189707862 28.91041477051099 17.96914828038752 12 22.754296343831896 29.742940256861853 29.30422916203071 18.198534237275542 13 23.210088170000997 29.530479028654945 28.981260330977506 18.278172470366556 14 22.319065544698358 30.328839866376363 28.916661570689268 18.435433018236015 15 21.550252421807173 30.418358278313583 29.05371508117065 18.97767421870859 16 22.36445409958461 30.060614529451378 29.370912012660728 18.20401935830328 17 22.15471570801613 31.028732460108966 28.721355412857076 18.095196419017828 18 21.60704343231864 30.676283844946646 29.300869016136637 18.41580370659808 19 22.239212763930148 30.054048173340973 29.01347329020477 18.6932657725241 20 22.260864742330792 30.496687140644713 28.63756792463832 18.604880192386183 21 21.621797354486887 30.425783595847854 28.917858081827312 19.034560967837947 22 22.661023095739235 30.10932789123386 28.220995221571194 19.008653791455714 23 22.17811193897542 30.377845634919513 28.3627146859377 19.081327740167367 24 21.617385009862495 30.32830656641575 29.20325696951942 18.851051454202334 25 22.473189233313757 30.1803691573434 28.541751698382605 18.80468991096024 26 21.940643131179492 30.457187465221686 28.669546830246457 18.93262257335237 27 21.24964041610486 30.578652850074008 29.178605923371265 18.99310081044987 28 22.345175499039545 30.099066627399473 28.862511656610813 18.69324621695017 29 21.868325600605548 30.409989923615544 28.88093474134869 18.84074973443022 30 21.33278059755194 30.161845872044985 29.141429727419727 19.363943802983353 31 21.798600336476724 30.35102514473761 28.63255490500861 19.217819613777056 32 21.560926320699576 30.66993852118054 28.77107068144358 18.998064476676305 33 20.97173825614719 30.774577407720344 28.96415925701381 19.28952507911866 34 21.99782484181395 30.191216954430118 28.60955199492013 19.201406208835806 35 21.66086429708132 30.772895538859522 28.241362931106817 19.32487723295234 36 21.30723513550413 30.445728661797165 29.025836518988786 19.221199683709923 37 21.66958443490343 30.338733693325732 28.889496460991264 19.102185410779576 38 21.837408190388015 30.752851262206615 28.568015376443405 18.841725170961958 39 20.944467119568696 30.746875928830764 29.313401188050097 18.995255763550443 40 21.62737172080483 30.58168420295925 28.653493008974184 19.13745106726174 41 21.807910769545828 30.640935025484662 28.618374271866752 18.932779933102758 42 21.09443106849136 30.53444477785568 29.359727177961485 19.01139697569148 43 21.818682162604954 30.60369592918743 28.932115673424807 18.645506234782808 44 21.5442852418599 30.664788557917905 28.931596047143522 18.859330153078677 45 20.925470035660766 30.89580794553603 29.44223372957592 18.736488289227285 46 21.80757558375262 30.70493530961388 28.89348336191914 18.594005744714355 47 21.568659595707246 30.87424984951551 28.948832422110687 18.60825813266656 48 21.207013821454694 30.551895691008568 29.350984812512742 18.890105675023992 49 21.659730683756965 30.53054030795907 29.09505207869162 18.714676929592347 50 21.630184906833538 30.76322199373091 28.898537972499277 18.708055126936273 51 21.456671581222782 30.569121283069876 29.082064056533557 18.892143079173785 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 41.0 1 69.5 2 98.0 3 142.0 4 186.0 5 292.0 6 398.0 7 589.5 8 781.0 9 1209.0 10 1637.0 11 2570.5 12 3504.0 13 5233.5 14 6963.0 15 10163.5 16 13364.0 17 18979.5 18 24595.0 19 33296.0 20 41997.0 21 54869.5 22 67742.0 23 84879.5 24 102017.0 25 121346.0 26 161427.0 27 182179.0 28 202089.5 29 222000.0 30 237880.5 31 253761.0 32 263005.5 33 272250.0 34 272614.5 35 272979.0 36 266369.5 37 259760.0 38 245575.0 39 231390.0 40 212916.5 41 194443.0 42 175153.5 43 155864.0 44 137359.0 45 118854.0 46 102290.5 47 85727.0 48 72194.0 49 58661.0 50 48403.5 51 38146.0 52 31103.0 53 24060.0 54 19489.0 55 14918.0 56 11874.5 57 8831.0 58 7168.0 59 5505.0 60 4467.5 61 3430.0 62 2818.5 63 2207.0 64 1784.5 65 1362.0 66 1136.0 67 910.0 68 751.0 69 592.0 70 486.5 71 381.0 72 294.5 73 208.0 74 165.5 75 95.5 76 68.0 77 49.0 78 30.0 79 25.5 80 21.0 81 16.5 82 12.0 83 7.5 84 3.0 85 3.0 86 3.0 87 1.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 100.0 2 48.68747769028498 3 100.0 4 87.80794517391979 5 36.49542286420476 6 62.943403363913944 7 36.45247444071055 8 4.6219329919265493E-4 9 36.37272831993447 10 11.508186509928624 11 23.983494721752525 12 11.965793429460058 13 13.469557105048716 14 0.0 15 13.4694859983873 16 12.4753082118239 17 24.441101641283957 18 0.0024887331494989114 19 0.0 20 0.0 21 11.965793429460058 22 0.001279919905456583 23 11.965793429460058 24 0.0 25 0.0 26 11.508506489904988 27 11.508186509928624 28 11.51021304977893 29 11.508186509928624 30 0.0 31 0.0 32 0.0 33 11.508364276582158 34 2.4887331494989113E-4 35 0.0011377065826280737 36 0.0024176264880846564 37 8.532799369710554E-4 38 0.0022043065038418927 39 0.0 40 11.966291176089959 41 1.422133228285092E-4 42 0.0 43 0.0024176264880846564 44 0.12326339756161037 45 0.19159689918070905 46 0.2105468244476079 47 0.23959389563533093 48 0.2656544870436552 49 0.2665788736420405 50 0.2774581928384215 51 0.2632368605555706 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 2812676.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.76779537214317 #Duplication Level Percentage of deduplicated Percentage of total 1 98.78695594429884 96.5818289419913 2 0.8448801528775682 1.6520413980103823 3 0.1837455213839309 0.5389318360563573 4 0.057769707697951915 0.2259206784368755 5 0.023140778331448522 0.11312114403305917 6 0.027894274201384725 0.16362970153052603 7 0.027626978866600257 0.1890720171606208 8 0.01291091717308636 0.10098175265959969 9 0.011492676894629053 0.10112523146210259 >10 0.023100015752582453 0.30628552470608583 >50 4.830325219544412E-4 0.02706177395308 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 1.7776665353563652E-4 0.0 0.0 5 2.4887331494989113E-4 0.0 0.0 6 7.110666141425461E-4 0.0 0.0 7 0.019198798581848746 0.0 0.0 8 0.019838758534577037 0.0 0.0 9 0.0208342517943766 3.55533307071273E-5 0.0 10 0.025171758140646133 3.55533307071273E-5 0.0 11 0.03729544391177654 3.55533307071273E-5 0.0 12 0.047463696494014954 3.55533307071273E-5 0.0 13 0.05606760252513976 3.55533307071273E-5 0.0 14 0.06047621553282355 3.55533307071273E-5 0.0 15 0.06388933528070777 3.55533307071273E-5 0.0 16 0.06801352164273454 3.55533307071273E-5 0.0 17 0.07210215467405418 3.55533307071273E-5 0.0 18 0.07807511423285156 3.55533307071273E-5 0.0 19 0.08557686701205543 3.55533307071273E-5 0.0 20 0.08934552006701092 3.55533307071273E-5 0.0 21 0.09428743303530161 3.55533307071273E-5 0.0 22 0.0990871326807638 3.55533307071273E-5 0.0 23 0.10431347229471151 3.55533307071273E-5 0.0 24 0.11053530516845879 3.55533307071273E-5 0.0 25 0.11785929129412702 3.55533307071273E-5 0.0 26 0.12585879070323067 3.55533307071273E-5 0.0 27 0.1321517302383922 3.55533307071273E-5 0.0 28 0.13951126969476754 3.55533307071273E-5 0.0 29 0.14459539598588675 3.55533307071273E-5 0.0 30 0.15021282223761287 7.11066614142546E-5 3.55533307071273E-5 31 0.1566479750956029 7.11066614142546E-5 3.55533307071273E-5 32 0.16311868128430007 7.11066614142546E-5 3.55533307071273E-5 33 0.17001602744148278 7.11066614142546E-5 3.55533307071273E-5 34 0.17908212677180024 7.11066614142546E-5 3.55533307071273E-5 35 0.18996144596818118 7.11066614142546E-5 3.55533307071273E-5 36 0.20108963847951203 7.11066614142546E-5 3.55533307071273E-5 37 0.21136455105387184 7.11066614142546E-5 3.55533307071273E-5 38 0.22441262342338755 7.11066614142546E-5 3.55533307071273E-5 39 0.2380295490842173 7.11066614142546E-5 3.55533307071273E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGATCG 545 1.4126288E-5 18.7847 6 ACCCGGG 180 9.0039975E-10 18.147543 15 GGTCGCC 145 9.640644E-11 16.187975 44 GGAACTG 525 0.0044331066 14.625231 4 TACCCGG 230 2.3166649E-8 14.202425 14 CCCGGGG 265 5.456968E-12 14.025071 16 CTCGGTG 495 0.0 13.593538 44 GTTACCC 225 2.7255192E-7 13.401261 12 GTTTGGG 670 0.0014373234 13.370079 5 GGAATGG 680 0.0016070604 13.173461 4 CGGGGCA 290 0.0 12.833352 18 TTACCCG 300 4.3364707E-9 12.563682 13 TCTCGGT 590 0.0 11.933712 43 GGGGCAC 300 3.6379788E-12 11.814833 19 GAGTCGC 250 0.004644792 11.438026 9 GGTTTTT 1030 2.47026E-4 11.181911 4 ATCTCGG 595 0.0 11.04414 42 GTCGCCG 220 4.865433E-8 10.669157 45 CCGGGGC 335 3.3396645E-9 10.4781 17 TCGGTGG 660 0.0 10.194972 45 >>END_MODULE