FastQCFastQC Report
Sun 19 Apr 2015
HCNNTADXX l01n02 rad27-frb.342000000088b9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCNNTADXX l01n02 rad27-frb.342000000088b9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2812676
Sequences flagged as poor quality0
Sequence length51
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCG5451.4126288E-518.78476
ACCCGGG1809.0039975E-1018.14754315
GGTCGCC1459.640644E-1116.18797544
GGAACTG5250.004433106614.6252314
TACCCGG2302.3166649E-814.20242514
CCCGGGG2655.456968E-1214.02507116
CTCGGTG4950.013.59353844
GTTACCC2252.7255192E-713.40126112
GTTTGGG6700.001437323413.3700795
GGAATGG6800.001607060413.1734614
CGGGGCA2900.012.83335218
TTACCCG3004.3364707E-912.56368213
TCTCGGT5900.011.93371243
GGGGCAC3003.6379788E-1211.81483319
GAGTCGC2500.00464479211.4380269
GGTTTTT10302.47026E-411.1819114
ATCTCGG5950.011.0441442
GTCGCCG2204.865433E-810.66915745
CCGGGGC3353.3396645E-910.478117
TCGGTGG6600.010.19497245
Produced by FastQC (version 0.11.2)