##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename HCNNTADXX l01n02 dna2-frb rnh201-frb.3420000000890b.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2224612 Sequences flagged as poor quality 0 Sequence length 51 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 2.0 2.0 2.0 2.0 2.0 2.0 2 7.782250567739453 6.0 2.0 15.0 2.0 15.0 3 2.0 2.0 2.0 2.0 2.0 2.0 4 3.3442438501635343 2.0 2.0 2.0 2.0 6.0 5 9.45656186337213 15.0 2.0 15.0 2.0 15.0 6 7.13357790032599 2.0 2.0 15.0 2.0 15.0 7 12.111485508484176 15.0 2.0 15.0 2.0 27.0 8 17.22059082662505 15.0 15.0 22.0 6.0 27.0 9 12.63689847937528 15.0 2.0 15.0 2.0 27.0 10 17.4710628190444 15.0 15.0 27.0 2.0 33.0 11 17.738895142164118 22.0 6.0 27.0 2.0 33.0 12 18.609659572096167 15.0 6.0 27.0 2.0 33.0 13 18.330723290173747 15.0 15.0 27.0 2.0 33.0 14 21.077150981834137 22.0 15.0 27.0 6.0 33.0 15 19.84434499139625 22.0 6.0 27.0 2.0 33.0 16 20.543646262808974 22.0 15.0 33.0 2.0 33.0 17 19.183968710049214 22.0 6.0 33.0 2.0 33.0 18 21.99032415540328 22.0 15.0 33.0 6.0 33.0 19 22.781216679582776 27.0 15.0 33.0 6.0 33.0 20 24.10594611554734 27.0 15.0 33.0 6.0 33.0 21 21.016986782414193 27.0 6.0 33.0 2.0 33.0 22 22.65235375876782 27.0 15.0 33.0 6.0 33.0 23 21.52571594507267 27.0 15.0 33.0 2.0 33.0 24 23.58815379940412 27.0 15.0 33.0 6.0 33.0 25 23.529530093337623 27.0 15.0 33.0 6.0 33.0 26 21.925822120891194 27.0 15.0 33.0 2.0 33.0 27 21.785376955621924 27.0 15.0 33.0 2.0 33.0 28 22.198912439562495 27.0 15.0 33.0 2.0 33.0 29 21.772561237645036 27.0 15.0 33.0 2.0 33.0 30 22.327972248643807 27.0 15.0 33.0 6.0 33.0 31 22.349921244693455 27.0 15.0 33.0 6.0 33.0 32 23.73300377773742 27.0 15.0 33.0 6.0 33.0 33 21.57151943799638 27.0 15.0 33.0 2.0 33.0 34 22.60524666773352 27.0 15.0 33.0 6.0 33.0 35 21.766444215890232 27.0 15.0 27.0 6.0 33.0 36 22.633753211796034 27.0 15.0 27.0 6.0 33.0 37 23.17343563731563 27.0 15.0 33.0 6.0 33.0 38 24.167579784699534 27.0 22.0 33.0 6.0 33.0 39 24.094727080497634 27.0 15.0 33.0 6.0 33.0 40 20.493539997087133 27.0 6.0 27.0 2.0 33.0 41 22.27813389480952 27.0 15.0 27.0 6.0 33.0 42 23.48425523192359 27.0 15.0 33.0 6.0 33.0 43 23.80155505769096 27.0 15.0 33.0 6.0 33.0 44 24.01846164634552 27.0 22.0 33.0 6.0 33.0 45 24.564016107078448 27.0 22.0 33.0 6.0 33.0 46 24.76350482690914 27.0 22.0 33.0 6.0 33.0 47 24.998796644088948 27.0 22.0 33.0 6.0 33.0 48 24.96688231475871 27.0 22.0 33.0 6.0 33.0 49 25.194098566401692 27.0 22.0 33.0 6.0 33.0 50 25.358803692509074 27.0 22.0 33.0 15.0 33.0 51 23.994588719291276 27.0 22.0 33.0 6.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2201 1 0.0 2201 2 -5.676743014091253 2201 3 0.0 2201 4 9.63576992320976 2201 5 3.6719620396045283 2201 6 11.53718161002956 2201 7 11.16090374051128 2201 8 5.583693795894632 2201 9 10.144851676818565 2201 10 4.8958258035030475 2201 11 8.296398667542537 2201 12 7.213407276016575 2201 13 7.983021137331431 2201 14 6.413344941035888 2201 15 3.150761605966064 2201 16 4.016344165545075 2201 17 6.786282402986476 2201 18 2.5599318285179713 2201 19 2.5359040373904556 2201 20 1.6550549211148073 2201 21 5.286797199219393 2201 22 4.423584705893717 2201 23 2.6685305982227625 2201 24 2.5160428394482715 2201 25 -0.13125421075653776 2201 26 3.8186605010862813 2201 27 4.352034432113992 2201 28 2.9355872283472912 2201 29 4.274104699474368 2201 30 2.2259119194290804 2201 31 2.784304908034553 2201 32 1.3950759566301194 2201 33 2.0993754276921948 2201 34 -0.368741785017459 2201 35 -3.395848986811348 2201 36 -2.3189690356451855 2201 37 -0.6481746960498569 2201 38 -0.3438621028997524 2201 39 0.37469027087178475 2201 40 3.1404683324073446 2201 41 0.24312223074544548 2201 42 -1.814430379306991 2201 43 -2.311991171911611 2201 44 -1.4698534691144118 2201 45 -2.980669159893978 2201 46 -1.9197658630190801 2201 47 -0.673878789079307 2201 48 -0.6373880075155114 2201 49 -0.7822119299226884 2201 50 -1.201669829210445 2201 51 -1.314784601470567 1101 1 0.0 1101 2 6.474834938206595 1101 3 0.0 1101 4 -1.376538560458537 1101 5 4.514202207677801 1101 6 7.899202207505863 1101 7 8.342783583130089 1101 8 3.7722605314130604 1101 9 -10.429311530000703 1101 10 -4.56677495014511 1101 11 0.1774565918824642 1101 12 3.2943938280793574 1101 13 6.842293605040428 1101 14 1.979935575112652 1101 15 2.594911598154969 1101 16 5.48926584612904 1101 17 6.131694831234547 1101 18 3.2534086241947513 1101 19 0.47383782880290326 1101 20 0.9467640350204718 1101 21 0.2262547884015298 1101 22 1.9082272333746282 1101 23 3.9692207757678837 1101 24 1.1971224243704626 1101 25 0.6879788337469463 1101 26 0.8021185041313359 1101 27 -0.39888442938017477 1101 28 2.1204455312746404 1101 29 2.640039082003529 1101 30 2.4136229453267077 1101 31 1.8388845351060823 1101 32 0.5573019127132 1101 33 2.343415052517752 1101 34 2.757248951574965 1101 35 1.7236511067315448 1101 36 0.8220127085180238 1101 37 1.413818300234528 1101 38 0.9497093300151569 1101 39 0.8663947859146646 1101 40 1.150282114025412 1101 41 -0.4578852114237648 1101 42 1.355858727388398 1101 43 0.6033220309759244 1101 44 -1.2604708240026419 1101 45 -2.4050192296787074 1101 46 -2.121350333823532 1101 47 -0.9869365798643877 1101 48 -0.24493397535723105 1101 49 -1.5533855491785928 1101 50 -1.05639427597524 1101 51 -1.233162899782009 1102 1 0.0 1102 2 6.065563561609139 1102 3 0.0 1102 4 -1.376538560458537 1102 5 4.3141575010014765 1102 6 6.5882566865292125 1102 7 7.820063632066979 1102 8 6.725487926157832 1102 9 -10.429311530000703 1102 10 -3.0029010225923454 1102 11 -15.544656555296129 1102 12 -6.146652549022651 1102 13 -2.4963355968952605 1102 14 -1.0141890710360215 1102 15 -1.4626044265401212 1102 16 -18.616973246366598 1102 17 -17.08401924671775 1102 18 -8.004987251408 1102 19 -5.237890067453794 1102 20 -3.313908784097208 1102 21 0.03730946358790277 1102 22 1.053623749314962 1102 23 4.717602657760835 1102 24 2.4717478610822994 1102 25 0.7600353845575185 1102 26 4.660821488232841 1102 27 4.0461825877790645 1102 28 4.333251458615283 1102 29 1.1938593009562517 1102 30 0.886284307147335 1102 31 -0.8968924581462652 1102 32 0.050516338785197235 1102 33 2.385054602158526 1102 34 0.0150275585911821 1102 35 0.6496553930160687 1102 36 2.0135830965484125 1102 37 1.0634067083255623 1102 38 0.7996266035585435 1102 39 -1.4752948980298015 1102 40 2.0519245708562472 1102 41 0.3431508521306874 1102 42 -0.2380356636495442 1102 43 0.9413734682057431 1102 44 1.107754426713207 1102 45 0.6669834195776687 1102 46 1.023017413453978 1102 47 0.060916347013755257 1102 48 1.3541608944181327 1102 49 0.05023547310520726 1102 50 0.4683008480098394 1102 51 0.6150320573420558 1103 1 0.0 1103 2 5.06983783868429 1103 3 0.0 1103 4 -1.376538560458537 1103 5 4.7985963857556655 1103 6 -5.204928100812928 1103 7 1.3267752043883085 1103 8 -0.4759885347945847 1103 9 8.959767311511765 1103 10 6.929620220577078 1103 11 7.031972567793172 1103 12 6.901958113616466 1103 13 -16.374365789956663 1103 14 -7.433839184249491 1103 15 -17.961080257575105 1103 16 -6.914237928587033 1103 17 -0.43059339528305074 1103 18 0.12727244776146307 1103 19 1.1409593111985608 1103 20 0.999945650788959 1103 21 2.5982481314600605 1103 22 0.48755351406598635 1103 23 1.887396621001482 1103 24 1.7258466307113096 1103 25 0.29268973486300354 1103 26 2.0931227644053934 1103 27 2.1341576159632183 1103 28 1.0880452122337942 1103 29 3.756931404430876 1103 30 0.23829772677881422 1103 31 -0.6029492746590854 1103 32 -0.024717050256629847 1103 33 2.230121914025382 1103 34 0.26598022015194545 1103 35 1.3204236320589509 1103 36 0.7661401362494438 1103 37 1.4081296666906375 1103 38 -1.078080996218965 1103 39 -0.15285852418676527 1103 40 4.314903616311398 1103 41 1.8515696965027395 1103 42 1.2609143863736563 1103 43 1.7521014142541027 1103 44 1.3891757482855915 1103 45 1.5862346424999672 1103 46 0.9371181893828613 1103 47 -0.23470944824143558 1103 48 -0.47056393711632083 1103 49 -0.20330283466843468 1103 50 0.10785197189005657 1103 51 0.6526480487432273 2103 1 0.0 2103 2 5.096735717864988 2103 3 0.0 2103 4 -1.376538560458537 2103 5 4.779548541356441 2103 6 -5.204928100812928 2103 7 -10.043196637928666 2103 8 -3.5624161279320727 2103 9 2.927544224778927 2103 10 3.092085891463608 2103 11 5.497075645605623 2103 12 5.599374912588829 2103 13 5.775436733548283 2103 14 3.731850307044006 2103 15 4.781046896316756 2103 16 5.273278754612047 2103 17 6.814131814063067 2103 18 2.266613658193865 2103 19 0.5669892541965353 2103 20 1.8121258475880744 2103 21 4.528874982077266 2103 22 1.1160889603931778 2103 23 2.921546220929475 2103 24 1.7178685045311148 2103 25 0.9321395504082943 2103 26 5.119368935582205 2103 27 4.659282591102738 2103 28 2.75234928273332 2103 29 3.1364823800906514 2103 30 0.6664484658812633 2103 31 3.8690394214392967 2103 32 -0.2510225386154268 2103 33 3.6081070274309255 2103 34 1.0785410954635317 2103 35 -0.2131178720286826 2103 36 0.6018112052701774 2103 37 -2.3580920476509277 2103 38 -0.20390985930553995 2103 39 -1.2253257400573894 2103 40 1.2600637951274543 2103 41 0.9173968831501185 2103 42 -0.6029808664887426 2103 43 1.015032536729123 2103 44 0.44661347027073006 2103 45 0.8808325259800185 2103 46 0.6821510036695884 2103 47 1.025244873430129 2103 48 0.6842310192142911 2103 49 0.7838829605715283 2103 50 1.0982767435370775 2103 51 0.8296473184714692 2102 1 0.0 2102 2 -5.676743014091253 2102 3 0.0 2102 4 -1.376538560458537 2102 5 -7.359488891798639 2102 6 -5.204928100812928 2102 7 1.479063753689335 2102 8 -4.328280289956368 2102 9 7.671169404386106 2102 10 3.4909943704136666 2102 11 5.448644715744752 2102 12 3.9895982306893423 2102 13 7.915568940376556 2102 14 3.8672353456566313 2102 15 6.380898443902051 2102 16 3.55465162481169 2102 17 7.720515277500731 2102 18 4.5818875123222504 2102 19 2.200172621066468 2102 20 1.2625629099825275 2102 21 2.5764006164765796 2102 22 -1.5060333250096605 2102 23 1.0189289722359547 2102 24 -1.9549432643603133 2102 25 1.1242682693774562 2102 26 2.6710702286386265 2102 27 2.427657044665935 2102 28 3.612970367361065 2102 29 1.299182348432506 2102 30 -0.4997254169564158 2102 31 0.9907053826505212 2102 32 -0.2956610790599967 2102 33 2.4867226684712804 2102 34 1.611721215713576 2102 35 1.828107958228017 2102 36 -0.6057485275229197 2102 37 2.1008036254008076 2102 38 -0.4836227618136135 2102 39 -0.4124712492983029 2102 40 2.0901859416364346 2102 41 3.0293460640395686 2102 42 2.5927808357419195 2102 43 3.0863048278693768 2102 44 0.7237117883787505 2102 45 1.6828521300650507 2102 46 1.471325601111836 2102 47 1.2878012953073004 2102 48 0.9583844189542106 2102 49 1.4591040191854923 2102 50 0.9937993737564028 2102 51 1.0504189667773822 2101 1 0.0 2101 2 -5.676743014091253 2101 3 0.0 2101 4 -1.376538560458537 2101 5 -7.359488891798639 2101 6 -5.204928100812928 2101 7 -10.043196637928666 2101 8 -4.176409975675469 2101 9 -10.429311530000703 2101 10 -15.276199033914743 2101 11 -15.544656555296129 2101 12 -4.464281958837276 2101 13 -3.9956603131278623 2101 14 -2.3747534207875844 2101 15 1.623370144569666 2101 16 3.3366077570803583 2101 17 7.146007562933722 2101 18 2.8280096515527156 2101 19 3.386059221648182 2101 20 1.4920603286802887 2101 21 3.6271171566119165 2101 22 1.293763520537567 2101 23 2.2242622206639453 2101 24 1.9662909742630816 2101 25 1.463859797490752 2101 26 -19.728277074693562 2101 27 -19.624524336302805 2101 28 -20.03185958798074 2101 29 -19.608615659125036 2101 30 -8.296354916046027 2101 31 -8.550937569945063 2101 32 -3.710232862076147 2101 33 -19.436980526307146 2101 34 -9.56360816855647 2101 35 -4.252010968896492 2101 36 -3.7659196898529466 2101 37 -1.660219862022128 2101 38 -0.7888557815892341 2101 39 0.03451022760996736 2101 40 4.367667584254846 2101 41 2.165865211619746 2101 42 1.2967856137725597 2101 43 -2.0908766945351758 2101 44 -0.4678686101176375 2101 45 0.5836070157513653 2101 46 -0.4394450188151424 2101 47 -0.6524298647518592 2101 48 -0.9798922692269265 2101 49 -0.258424114370964 2101 50 -0.7950483269775219 2101 51 -0.41629430573021864 2104 1 0.0 2104 2 -5.676743014091253 2104 3 0.0 2104 4 -1.376538560458537 2104 5 -7.359488891798639 2104 6 -5.204928100812928 2104 7 -10.043196637928666 2104 8 -3.538347325107022 2104 9 1.5846019725067446 2104 10 4.437348720694811 2104 11 4.637764922023731 2104 12 -16.38779785313065 2104 13 -5.649958716316906 2104 14 -5.169584492776078 2104 15 0.8926959952057096 2104 16 3.86106302677544 2104 17 -17.08401924671775 2104 18 -7.612136471134999 2104 19 -5.066032206849307 2104 20 -4.854604909077942 2104 21 -18.881002337834655 2104 22 -8.776808358570369 2104 23 -19.40748806658232 2104 24 -9.639975970046212 2104 25 -5.12971735968744 2104 26 0.5631146526168713 2104 27 2.4040944940580395 2104 28 3.1892105074153605 2104 29 3.3080164437368467 2104 30 2.3655149684392605 2104 31 0.5678450555199603 2104 32 2.278739321879705 2104 33 4.284183834011092 2104 34 4.203830912078718 2104 35 2.339139737701956 2104 36 2.4870901064350335 2104 37 -1.3196716949286262 2104 38 1.148995568253401 2104 39 1.9903551271758353 2104 40 -18.375495954619147 2104 41 -8.092565726764557 2104 42 -3.8508926538312522 2104 43 -2.995266411587476 2104 44 -0.4690625304135736 2104 45 -0.014821344301385153 2104 46 0.3669490080395086 2104 47 0.17399216618577995 2104 48 -0.663998143370641 2104 49 0.5041019752784663 2104 50 0.38488349496983787 2104 51 -0.18350458435133277 >>END_MODULE >>Per sequence quality scores fail #Quality Count 5 8.0 6 188.0 7 2144.0 8 8512.0 9 20480.0 10 37162.0 11 54904.0 12 72221.0 13 90015.0 14 106647.0 15 121776.0 16 136473.0 17 149752.0 18 159713.0 19 167631.0 20 169492.0 21 152096.0 22 135759.0 23 129836.0 24 129790.0 25 124832.0 26 109534.0 27 85603.0 28 44830.0 29 12792.0 30 2422.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 NaN NaN NaN NaN 2 38.97016974120325 28.01972120364187 26.44371650945973 6.566392545695149 3 NaN NaN NaN NaN 4 39.39076633673552 23.022066441813813 27.381713437529854 10.205453783920817 5 37.496588971006254 26.67495736213758 25.88224843661171 9.946205230244457 6 13.46561007328901 34.48071757599329 40.20880683544611 11.844865515271582 7 22.309556838194215 30.445555246343808 30.92325199176033 16.321635923701646 8 22.307331308696053 32.038491483397436 29.04801590940557 16.60616129850094 9 22.73825254454772 31.363280235370222 29.25396500729339 16.644502212788666 10 23.46391391348058 29.381318076325442 28.650332338008454 18.504435672185515 11 23.279169460960066 30.644373932145474 28.489470154576757 17.586986452317703 12 22.39874451362662 30.191334081861793 29.370113299989793 18.039808104521793 13 22.51113668703223 29.714513539231614 29.267073354960388 18.507276418775774 14 22.228235755268784 30.726571644853124 28.59406494256077 18.451127657317322 15 21.614220481629992 30.235291430375042 28.993319808211837 19.157168279783125 16 22.06811382506756 30.058987379050734 29.175125868302736 18.697772927578974 17 22.095152237522488 31.40180621896619 28.22218619365513 18.280855349856193 18 21.468081844904294 30.92824657941902 29.010734310640046 18.592937265036642 19 21.841876246284745 30.25884963310456 28.974625687535628 18.92464843307507 20 22.04618153637578 30.688182928079144 28.78011985910352 18.485515676441555 21 21.139226293763397 30.59303868531183 29.411758701643357 18.85597631928141 22 21.947009346928702 30.14077707217674 28.823360444056657 19.0888531368379 23 21.809227314484026 30.51551495355721 28.868224966826578 18.807032765132185 24 21.09967940476811 30.518085850476396 29.6349206063799 18.74731413837559 25 21.970977410892324 30.290900165961528 28.784390266707184 18.953732156438967 26 21.701537963214783 30.91520983383804 28.69066863050075 18.69258357244643 27 21.021076997119962 30.844174859881385 29.146556048718754 18.988192094279903 28 21.67957678511312 30.450529095004857 29.031901548653977 18.83799257122805 29 21.623720725331864 30.875852743676518 28.88513711379847 18.615289417193146 30 21.06129068799413 30.427508257619756 29.158163311175162 19.35303774321095 31 21.27175435536624 30.52356995287268 28.83752312762855 19.36715256413253 32 21.38498758435179 30.961623869690534 28.8245770498406 18.828811496117076 33 20.855192560673203 30.767385565958122 29.16500085815664 19.212421015212037 34 21.553893837332918 30.331676859487416 28.82916293004826 19.28526637313141 35 21.46938166361141 31.086244516947453 28.336019980382833 19.108353839058303 36 21.07386599129169 30.512977502784842 29.251908932265607 19.161247573657857 37 21.309958828349863 30.3507749071851 29.033138256100337 19.30612800836469 38 21.572745503233698 31.06710007403768 28.61405690966639 18.746097513062235 39 20.55931551209829 30.809867068954045 29.651597671863676 18.979219747083988 40 21.206190134001844 30.75351540490442 28.842856092960208 19.19743836813353 41 21.496297554943315 30.967886070868122 28.76395019174139 18.771866182447177 42 20.93507584022555 30.656640644139582 29.474456433057284 18.933827082577583 43 21.362799014993566 30.580736633050943 29.27063132654905 18.78583302540644 44 21.283590983475225 30.8366443491896 29.009989756404046 18.869774910931124 45 20.61342846449673 30.63579857978817 29.827195700902504 18.923577254812592 46 21.236353283950795 30.594049950719565 29.23203606570965 18.93756069961999 47 21.333519576986593 30.92454203211351 29.18058049443184 18.561357896468053 48 20.932970361289392 30.58650933871782 29.67553364101824 18.804986658974542 49 21.27769086647043 30.543097266598068 29.364724165144406 18.814487701787098 50 21.320626632324558 31.048537229846623 29.031689521839905 18.599146615988914 51 21.20176474803575 30.631962419105573 29.187749883156876 18.978522949701794 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 60.0 1 126.5 2 193.0 3 241.0 4 289.0 5 384.0 6 479.0 7 664.5 8 850.0 9 1180.5 10 1511.0 11 2329.5 12 3148.0 13 4593.5 14 6039.0 15 8655.0 16 11271.0 17 15853.0 18 20435.0 19 27768.0 20 35101.0 21 45369.0 22 55637.0 23 69492.5 24 83348.0 25 99644.0 26 132801.5 27 149663.0 28 165435.5 29 181208.0 30 193307.5 31 205407.0 32 212175.5 33 218944.0 34 218705.5 35 218467.0 36 211640.0 37 204813.0 38 192561.5 39 180310.0 40 165222.0 41 150134.0 42 134191.5 43 118249.0 44 103427.0 45 88605.0 46 75811.5 47 63018.0 48 52642.0 49 42266.0 50 34516.5 51 26767.0 52 21605.0 53 16443.0 54 13161.5 55 9880.0 56 7921.0 57 5962.0 58 4883.5 59 3805.0 60 3068.5 61 2332.0 62 1907.5 63 1483.0 64 1208.5 65 934.0 66 781.5 67 629.0 68 510.5 69 392.0 70 327.0 71 262.0 72 203.5 73 145.0 74 127.5 75 79.0 76 48.0 77 36.0 78 24.0 79 15.0 80 6.0 81 4.0 82 2.0 83 1.5 84 1.0 85 1.0 86 1.0 87 0.5 88 0.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 100.0 2 48.97887811447569 3 100.0 4 87.76514736052849 5 36.744025475004186 6 63.01512353614922 7 36.51958184168745 8 7.192265437748245E-4 9 36.486767130627726 10 11.736833209566433 11 24.123217891479502 12 11.92171938297555 13 13.410023860340589 14 0.0 15 13.409978908681605 16 12.386474585231042 17 24.30819396820659 18 0.0028319545161133715 19 0.0 20 0.0 21 11.92171938297555 22 0.0013935014285637226 23 11.92171938297555 24 0.0 25 0.0 26 11.737147871179333 27 11.73674330624846 28 11.739800019059503 29 11.73674330624846 30 0.0 31 0.0 32 0.0 33 11.736923112884403 34 3.1466161290148574E-4 35 0.0013485497695777961 36 0.0031466161290148575 37 0.0010338881566763103 38 0.003101664470028931 39 0.0 40 11.92279822279121 41 4.4951658985926534E-5 42 4.4951658985926534E-5 43 0.002607196221183739 44 0.122987738985495 45 0.19055008244134258 46 0.20965453751036137 47 0.23810893764845287 48 0.26485517474507914 49 0.2640909965423184 50 0.2764527027634482 51 0.2618434135930221 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 2224612.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.55325223580675 #Duplication Level Percentage of deduplicated Percentage of total 1 99.07307687536462 97.63973935075283 2 0.6797186450458897 1.3397696614917673 3 0.14000695197388377 0.41394421357945943 4 0.04781555799368878 0.18849514990991423 5 0.020438005796392558 0.10071159702243783 6 0.01066393266491655 0.06305791474507078 7 0.012819712190056766 0.08843970303399779 8 0.004816024724542915 0.037970791956140774 9 0.002859062316345513 0.025359290065862034 >10 0.007785231929701317 0.10251232744250313 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 1.348549769577796E-4 0.0 0.0 5 1.7980663594370614E-4 0.0 0.0 6 4.944682488451919E-4 0.0 0.0 7 0.024049137557470696 0.0 0.0 8 0.02454360580631589 0.0 0.0 9 0.025262832350090712 0.0 0.0 10 0.028499351797077424 0.0 0.0 11 0.03686036036845976 4.4951658985926534E-5 0.0 12 0.041894946174883534 4.4951658985926534E-5 0.0 13 0.0460754504605747 4.4951658985926534E-5 0.0 14 0.048233130091899175 4.4951658985926534E-5 0.0 15 0.05030090640525179 4.4951658985926534E-5 0.0 16 0.052908102626435534 4.4951658985926534E-5 0.0 17 0.056099670414436316 4.4951658985926534E-5 0.0 18 0.05942609317939488 8.990331797185307E-5 0.0 19 0.06356164580610012 8.990331797185307E-5 0.0 20 0.066078938709312 8.990331797185307E-5 4.4951658985926534E-5 21 0.0694503131332565 8.990331797185307E-5 4.4951658985926534E-5 22 0.07223731599038394 8.990331797185307E-5 4.4951658985926534E-5 23 0.07718199847883586 8.990331797185307E-5 4.4951658985926534E-5 24 0.08302571414700631 8.990331797185307E-5 8.990331797185307E-5 25 0.08900428479213454 8.990331797185307E-5 8.990331797185307E-5 26 0.09615159857089686 8.990331797185307E-5 8.990331797185307E-5 27 0.1031640573727014 8.990331797185307E-5 8.990331797185307E-5 28 0.11008661285653408 8.990331797185307E-5 8.990331797185307E-5 29 0.11557071525281712 8.990331797185307E-5 8.990331797185307E-5 30 0.12127957594402979 8.990331797185307E-5 8.990331797185307E-5 31 0.12973048783338398 8.990331797185307E-5 8.990331797185307E-5 32 0.1368778016121463 8.990331797185307E-5 8.990331797185307E-5 33 0.14460948695772566 8.990331797185307E-5 8.990331797185307E-5 34 0.154184190321728 8.990331797185307E-5 8.990331797185307E-5 35 0.1655120083861815 8.990331797185307E-5 8.990331797185307E-5 36 0.17683982645063498 8.990331797185307E-5 8.990331797185307E-5 37 0.18983085589756776 8.990331797185307E-5 8.990331797185307E-5 38 0.20358606354726128 8.990331797185307E-5 8.990331797185307E-5 39 0.21797059442275776 8.990331797185307E-5 8.990331797185307E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCCATC 160 0.0055902433 19.249252 7 CTCGGTG 380 0.0 16.462002 44 TCATGCG 475 1.2732926E-11 14.617381 9 ATCTCGG 400 0.0 13.682189 42 TCTCGGT 450 0.0 13.552186 43 TCGGTGG 485 0.0 12.2531595 45 ACCCGGG 165 5.852046E-4 12.139768 15 GATCTCG 455 0.0 11.679894 41 GTCGCCG 95 0.00439659 11.523401 45 GTAGATC 665 1.8189894E-12 11.501324 5 ACTTCAT 920 0.00124858 11.084609 6 GGGTGGC 195 0.005906309 11.075154 10 TACCCGG 205 2.9063283E-4 10.992411 14 GGTCGCC 120 0.002090754 10.425934 44 CCCGGGG 235 5.799043E-5 9.643794 16 GGGTTAC 290 0.0010359187 9.574825 10 CATGCGA 460 1.2389173E-6 9.389806 10 CCGGGGC 245 9.145692E-5 9.25017 17 TTCATGC 715 1.1713055E-6 8.914195 6 AGATCTC 595 0.0 8.644255 40 >>END_MODULE