FastQCFastQC Report
Sun 19 Apr 2015
HCNNTADXX l01n02 dna2-frb rnh201-frb.3420000000890b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCNNTADXX l01n02 dna2-frb rnh201-frb.3420000000890b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2224612
Sequences flagged as poor quality0
Sequence length51
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCATC1600.005590243319.2492527
CTCGGTG3800.016.46200244
TCATGCG4751.2732926E-1114.6173819
ATCTCGG4000.013.68218942
TCTCGGT4500.013.55218643
TCGGTGG4850.012.253159545
ACCCGGG1655.852046E-412.13976815
GATCTCG4550.011.67989441
GTCGCCG950.0043965911.52340145
GTAGATC6651.8189894E-1211.5013245
ACTTCAT9200.0012485811.0846096
GGGTGGC1950.00590630911.07515410
TACCCGG2052.9063283E-410.99241114
GGTCGCC1200.00209075410.42593444
CCCGGGG2355.799043E-59.64379416
GGGTTAC2900.00103591879.57482510
CATGCGA4601.2389173E-69.38980610
CCGGGGC2459.145692E-59.2501717
TTCATGC7151.1713055E-68.9141956
AGATCTC5950.08.64425540
Produced by FastQC (version 0.11.2)