##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename HCNNTADXX l01n02 dna2-frb rad27-frb.3420000000894f.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1617584 Sequences flagged as poor quality 0 Sequence length 51 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 2.0 2.0 2.0 2.0 2.0 2.0 2 7.774637360409104 6.0 2.0 15.0 2.0 15.0 3 2.0 2.0 2.0 2.0 2.0 2.0 4 3.3702484693221497 2.0 2.0 2.0 2.0 6.0 5 9.456898065262763 15.0 2.0 15.0 2.0 15.0 6 7.158648329854895 2.0 2.0 15.0 2.0 15.0 7 12.248592345127054 15.0 2.0 15.0 2.0 27.0 8 17.36858611361141 15.0 15.0 22.0 6.0 27.0 9 12.755233731293089 15.0 2.0 22.0 2.0 27.0 10 17.68567691075085 15.0 15.0 27.0 2.0 33.0 11 17.933759854202318 22.0 6.0 27.0 2.0 33.0 12 18.745422803390735 15.0 15.0 27.0 2.0 33.0 13 18.461378203543063 15.0 15.0 27.0 2.0 33.0 14 21.24795435662074 22.0 15.0 27.0 6.0 33.0 15 20.00167842906458 22.0 6.0 27.0 2.0 33.0 16 20.71381517126777 22.0 15.0 33.0 2.0 33.0 17 19.304688968239052 27.0 6.0 33.0 2.0 33.0 18 22.192840680916724 22.0 15.0 33.0 6.0 33.0 19 22.976142815458115 27.0 15.0 33.0 6.0 33.0 20 24.343471498234404 27.0 22.0 33.0 6.0 33.0 21 21.163024609541143 27.0 6.0 33.0 2.0 33.0 22 22.86356133591826 27.0 15.0 33.0 6.0 33.0 23 21.70059731055698 27.0 15.0 33.0 2.0 33.0 24 23.821265541696754 27.0 15.0 33.0 6.0 33.0 25 23.768732257490182 27.0 15.0 33.0 6.0 33.0 26 22.16197551410004 27.0 15.0 33.0 2.0 33.0 27 22.016859093561756 27.0 15.0 33.0 2.0 33.0 28 22.478814083225352 27.0 15.0 33.0 2.0 33.0 29 22.034926779691194 27.0 15.0 33.0 2.0 33.0 30 22.56632236718464 27.0 15.0 33.0 6.0 33.0 31 22.56134210031751 27.0 15.0 33.0 6.0 33.0 32 24.003262272623864 27.0 15.0 33.0 6.0 33.0 33 21.8086974154047 27.0 15.0 33.0 2.0 33.0 34 22.857129521558075 27.0 15.0 33.0 6.0 33.0 35 21.9785680372704 27.0 15.0 27.0 6.0 33.0 36 22.881619130753023 27.0 15.0 33.0 6.0 33.0 37 23.44087107686525 27.0 15.0 33.0 6.0 33.0 38 24.41802589540945 27.0 22.0 33.0 6.0 33.0 39 24.351783276788098 27.0 22.0 33.0 6.0 33.0 40 20.64638745190358 27.0 6.0 33.0 2.0 33.0 41 22.467372328114028 27.0 15.0 27.0 6.0 33.0 42 23.730746594921808 27.0 15.0 33.0 6.0 33.0 43 24.05011362624754 27.0 22.0 33.0 6.0 33.0 44 24.28944030109101 27.0 22.0 33.0 6.0 33.0 45 24.818285789176944 27.0 22.0 33.0 6.0 33.0 46 25.02140414346334 27.0 22.0 33.0 6.0 33.0 47 25.26156354167697 27.0 22.0 33.0 15.0 33.0 48 25.242399158250823 27.0 22.0 33.0 15.0 33.0 49 25.46016342891621 27.0 22.0 33.0 15.0 33.0 50 25.62084874726753 27.0 22.0 33.0 15.0 33.0 51 24.23047705714201 27.0 22.0 33.0 6.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2201 1 0.0 2201 2 -5.718336965472984 2201 3 0.0 2201 4 9.681312527997612 2201 5 3.643477929096152 2201 6 11.511799710982068 2201 7 11.242616146995172 2201 8 5.789462926717999 2201 9 10.21667494047194 2201 10 4.947057365309597 2201 11 8.229147023406664 2201 12 7.401497674281252 2201 13 8.01815409387683 2201 14 6.5052966811414095 2201 15 3.2904124986563232 2201 16 4.071559970547888 2201 17 6.79147811376674 2201 18 2.5289804820040267 2201 19 2.5840113600053343 2201 20 1.549754040163073 2201 21 5.31053188454187 2201 22 4.508292336585324 2201 23 2.677767344006824 2201 24 2.4792680007751713 2201 25 -0.11539719858579645 2201 26 3.8112850063038373 2201 27 4.357916737293799 2201 28 2.98866733010221 2201 29 4.2823717477881615 2201 30 2.235236437126325 2201 31 2.8974768558621022 2201 32 1.4359113172458535 2201 33 2.0630676726110657 2201 34 -0.34985512393673446 2201 35 -3.4573249957003895 2201 36 -2.2738034314754003 2201 37 -0.582543031909939 2201 38 -0.3234711264672434 2201 39 0.36400652254933874 2201 40 3.1032373511396116 2201 41 0.17669899951642876 2201 42 -1.7395333526786096 2201 43 -2.2954177913795135 2201 44 -1.5282504280980156 2201 45 -3.1191997685657427 2201 46 -2.1297012697628226 2201 47 -0.8206866516741158 2201 48 -0.6790647407279593 2201 49 -0.7524296942523847 2201 50 -1.2218632842163686 2201 51 -1.3489171374154978 1101 1 0.0 1101 2 6.503548890606421 1101 3 0.0 1101 4 -1.3830446468568018 1101 5 4.564403813098137 1101 6 7.79444485177014 1101 7 8.30924419286043 1101 8 3.708844104641326 1101 9 -10.527090924840888 1101 10 -4.683225478026179 1101 11 0.09293432221446096 1101 12 3.3043157354774984 1101 13 6.947635383290397 1101 14 1.926106636045109 1101 15 2.657498008964076 1101 16 5.460371389756741 1101 17 6.107374109566496 1101 18 3.317040735888554 1101 19 0.4886117755093302 1101 20 0.8662536098011202 1101 21 0.12565110696816717 1101 22 1.8239708578742544 1101 23 3.8856803892786296 1101 24 1.1373941545669801 1101 25 0.6357808544243717 1101 26 0.7448165815570285 1101 27 -0.46566975804817545 1101 28 2.17465355185033 1101 29 2.6362182919180768 1101 30 2.39258591635069 1101 31 1.6955203905336909 1101 32 0.45023321217726675 1101 33 2.3184603826785235 1101 34 2.7147330964347702 1101 35 1.6864342174974283 1101 36 0.7016892406381956 1101 37 1.3193585960797272 1101 38 0.8205465607976379 1101 39 0.7518005732662196 1101 40 0.9809266390730649 1101 41 -0.5995645768932896 1101 42 1.337832063956096 1101 43 0.528214152259558 1101 44 -1.2731712171677962 1101 45 -2.3689082352408803 1101 46 -2.1282748493059245 1101 47 -1.089314580701263 1101 48 -0.25729201259629164 1101 49 -1.6095589274571829 1101 50 -1.108094075554984 1101 51 -1.3360386476950907 1102 1 0.0 1102 2 6.101417471073221 1102 3 0.0 1102 4 -1.3830446468568018 1102 5 4.3148487745895405 1102 6 6.499198672193615 1102 7 7.910856460844585 1102 8 6.786443976655551 1102 9 -10.527090924840888 1102 10 -3.158729022982971 1102 11 -15.731631434609362 1102 12 -6.3362750145899405 1102 13 -2.625854517169085 1102 14 -1.0634824563499876 1102 15 -1.5552125449654142 1102 16 -18.800472282699836 1102 17 -17.288109761401245 1102 18 -8.164683813216303 1102 19 -5.331820719210455 1102 20 -3.4588590271782564 1102 21 -0.014006042627297433 1102 22 1.007280861598126 1102 23 4.760005441322189 1102 24 2.501441078079324 1102 25 0.6523295507543168 1102 26 4.659374692044917 1102 27 4.027252845712379 1102 28 4.316041329134855 1102 29 1.1265790709389663 1102 30 0.7892112709296306 1102 31 -0.999100195662372 1102 32 -0.07745140847430321 1102 33 2.4852481210591186 1102 34 -0.05686001932192397 1102 35 0.5527216987593384 1102 36 1.9642367545552304 1102 37 0.9322945436154306 1102 38 0.8407351029871215 1102 39 -1.5029958330175646 1102 40 2.180094025988641 1102 41 0.431642758457933 1102 42 -0.21777147173726874 1102 43 0.8043989377655905 1102 44 1.0696509999094488 1102 45 0.6129162799309711 1102 46 0.9348139447722907 1102 47 -0.04343119947399643 1102 48 1.267536398765479 1102 49 -0.160350143754993 1102 50 0.3679394953011652 1102 51 0.49413831089369253 1103 1 0.0 1103 2 5.096754435085957 1103 3 0.0 1103 4 -1.3830446468568018 1103 5 4.814552622577962 1103 6 -5.161088646989165 1103 7 1.3378851037180972 1103 8 -0.41043615937024924 1103 9 9.06020920802208 1103 10 7.000777199923743 1103 11 7.116492448654487 1103 12 6.8439274031811586 1103 13 -16.54035469115129 1103 14 -7.513764964236875 1103 15 -18.18801067589945 1103 16 -7.049429995624196 1103 17 -0.3682856902966485 1103 18 0.15839522877026369 1103 19 1.1355754502714248 1103 20 1.048590845275978 1103 21 2.5884262729729812 1103 22 0.5424810191571865 1103 23 1.8892349392071708 1103 24 1.7629689605560124 1103 25 0.288959530730871 1103 26 2.28221388616792 1103 27 2.1819171460082103 1103 28 1.1139300324447987 1103 29 3.813515069954157 1103 30 0.29315546841479545 1103 31 -0.6152522393993465 1103 32 0.010203624673184919 1103 33 2.2273791382298747 1103 34 0.35416856922150686 1103 35 1.3947069545780195 1103 36 0.7858194020098033 1103 37 1.4448089631155234 1103 38 -1.0538429272143972 1103 39 -0.10478542483911468 1103 40 4.427677201043515 1103 41 1.9291464749705618 1103 42 1.24107394993041 1103 43 1.7722063075806567 1103 44 1.4842227178372376 1103 45 1.6255883707679537 1103 46 1.0732690508946234 1103 47 -0.1713652223303761 1103 48 -0.4093430236997371 1103 49 -0.1714018653987992 1103 50 0.13707206227076085 1103 51 0.7001114889365141 2103 1 0.0 2103 2 5.171627065126337 2103 3 0.0 2103 4 -1.3830446468568018 2103 5 4.8514409052147744 2103 6 -5.161088646989165 2103 7 -10.103907171903838 2103 8 -3.6097706466471067 2103 9 2.9464892970548515 2103 10 3.3871678848883917 2103 11 5.8428657536980495 2103 12 5.827830860966873 2103 13 6.026543327998617 2103 14 3.971456466217191 2103 15 4.9453800733786295 2103 16 5.527203891371798 2103 17 7.101879599761936 2103 18 2.4801012789532173 2103 19 0.7266097030543648 2103 20 2.008312681681719 2103 21 4.842638914481526 2103 22 1.2590794166255108 2103 23 3.0773645901848212 2103 24 1.7915438930210534 2103 25 1.115835048002932 2103 26 5.26224481403554 2103 27 4.893416272466418 2103 28 2.9383968309226205 2103 29 3.280786854588971 2103 30 0.8222981121183608 2103 31 4.170277426427791 2103 32 -0.17644547823132584 2103 33 3.807869184917468 2103 34 1.1893541593705983 2103 35 -0.15875264978060244 2103 36 0.848024194666678 2103 37 -2.1936440587896406 2103 38 -0.02299710337464944 2103 39 -1.038431430813823 2103 40 1.429342530978559 2103 41 1.0016693496696973 2103 42 -0.570699071329603 2103 43 1.348511202461971 2103 44 0.5936635773975603 2103 45 1.0647732938250911 2103 46 0.8397420717621529 2103 47 1.1593667528856706 2103 48 0.7632364370210887 2103 49 0.9105992961700871 2103 50 1.255138501636921 2103 51 1.0314100717411954 2102 1 0.0 2102 2 -5.718336965472984 2102 3 0.0 2102 4 -1.3830446468568018 2102 5 -7.396241348192186 2102 6 -5.161088646989165 2102 7 1.5111196112932301 2102 8 -4.40205874945805 2102 9 7.814042427287671 2102 10 3.520843473869469 2102 11 5.545457184881286 2102 12 4.092043567546121 2102 13 8.08937935640063 2102 14 3.8858950894801687 2102 15 6.406717263204019 2102 16 3.5094609942478208 2102 17 7.758784327500173 2102 18 4.619662837815113 2102 19 2.2830119967613207 2102 20 1.4234926470658031 2102 21 2.5142019927106816 2102 22 -1.5737628855960963 2102 23 1.102131216326871 2102 24 -1.8932579814778094 2102 25 1.1383249584948913 2102 26 2.6715317800157266 2102 27 2.4906364810107746 2102 28 3.6760272543442873 2102 29 1.4638327789798034 2102 30 -0.3798874444479239 2102 31 1.040675352041955 2102 32 -0.19342998670024159 2102 33 2.5931235178947816 2102 34 1.731174675112495 2102 35 2.062715845537106 2102 36 -0.6185523036671192 2102 37 2.1464987018973183 2102 38 -0.5459595582610248 2102 39 -0.45428211220313486 2102 40 2.0849223828638195 2102 41 3.078273435775067 2102 42 2.57494865242203 2102 43 3.0187943814074103 2102 44 0.7044756485183932 2102 45 1.6228492186635926 2102 46 1.517393804850954 2102 47 1.3395572650062562 2102 48 0.9894998312083843 2102 49 1.4911680047258642 2102 50 0.9999103634763102 2102 51 0.9906005956641444 2101 1 0.0 2101 2 -5.718336965472984 2101 3 0.0 2101 4 -1.3830446468568018 2101 5 -7.396241348192186 2101 6 -5.161088646989165 2101 7 -10.103907171903838 2101 8 -4.25456820944758 2101 9 -10.527090924840888 2101 10 -15.48004929936613 2101 11 -15.731631434609362 2101 12 -4.582023207998027 2101 13 -4.092267841063482 2101 14 -2.4848318141104677 2101 15 1.6407903458662894 2101 16 3.4213360297901865 2101 17 7.184989062503817 2101 18 2.8584590274443684 2101 19 3.4775080807333225 2101 20 1.6458348120898982 2101 21 3.734696327259332 2101 22 1.4055512432892137 2101 23 2.261440901312991 2101 24 2.065996232035829 2101 25 1.585526071929241 2101 26 -19.957168530105502 2101 27 -19.833064298991104 2101 28 -20.288919649142226 2101 29 -19.83613902818118 2101 30 -8.464917177958355 2101 31 -8.704139488350835 2101 32 -3.6723906536113944 2101 33 -19.629083041639095 2101 34 -9.744944580421514 2101 35 -4.32281739218373 2101 36 -3.7765824809623325 2101 37 -1.6532351367576332 2101 38 -0.7596286431864243 2101 39 0.1580206435099747 2101 40 4.3780345201623305 2101 41 2.236984246107216 2101 42 1.360344889839034 2101 43 -2.1119606582094725 2101 44 -0.43076500216571034 2101 45 0.6902873592923235 2101 46 -0.34685993828191997 2101 47 -0.5521749490234065 2101 48 -0.9234240701662202 2101 49 -0.13980747445575403 2101 50 -0.7402032223628972 2101 51 -0.3183345543596623 2104 1 0.0 2104 2 -5.718336965472984 2104 3 0.0 2104 4 -1.3830446468568018 2104 5 -7.396241348192186 2104 6 -5.161088646989165 2104 7 -10.103907171903838 2104 8 -3.607917243091876 2104 9 1.5438569016861106 2104 10 4.466157876384084 2104 11 4.636366136363765 2104 12 -16.551317018864932 2104 13 -5.823235112182614 2104 14 -5.226675638186549 2104 15 0.8024250307955576 2104 16 3.859970002609611 2104 17 -17.288109761401245 2104 18 -7.79795577765926 2104 19 -5.363507647124646 2104 20 -5.083379608899321 2104 21 -19.102140456307268 2104 22 -8.972892849533489 2104 23 -19.653624821639486 2104 24 -9.845354337556575 2104 25 -5.301358815750845 2104 26 0.5257017699805253 2104 27 2.347594574547685 2104 28 3.081203320343125 2104 29 3.23283521401304 2104 30 2.312317417466442 2104 31 0.5145418985470052 2104 32 2.2233693729209847 2104 33 4.1339350242482595 2104 34 4.162229223540784 2104 35 2.2423163212928685 2104 36 2.3691686242349554 2104 37 -1.4135385772507512 2104 38 1.0446176947189656 2104 39 1.8266670615480862 2104 40 -18.584234651249517 2104 41 -8.254850687603623 2104 42 -3.9861956604020925 2104 43 -3.064746531886204 2104 44 -0.6198262962310892 2104 45 -0.128306518673309 2104 46 0.23961718507063168 2104 47 0.17804858531124879 2104 48 -0.7511488198047154 2104 49 0.4317808044231555 2104 50 0.31010015944909597 2104 51 -0.21297012776530266 >>END_MODULE >>Per sequence quality scores fail #Quality Count 5 5.0 6 138.0 7 1341.0 8 5431.0 9 13237.0 10 23857.0 11 35330.0 12 48438.0 13 60900.0 14 73266.0 15 85624.0 16 97679.0 17 108030.0 18 116890.0 19 123858.0 20 125428.0 21 113747.0 22 102123.0 23 98306.0 24 97428.0 25 94340.0 26 82787.0 27 64807.0 28 33239.0 29 9619.0 30 1736.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 NaN NaN NaN NaN 2 37.51840641111338 26.480058753875273 29.777021222515636 6.2245136124957074 3 NaN NaN NaN NaN 4 41.84990891806608 22.645059178371277 25.194855612737534 10.310176290825112 5 40.32673140073015 25.551215750451068 24.04235675945085 10.079696089367935 6 14.00782658254692 33.18991225744884 39.62679593892482 13.175465221079419 7 21.916015572144882 30.097480544483833 31.335558845929324 16.65094503744196 8 22.40552827876808 30.680945663073217 29.966975235754283 16.946550822404415 9 22.42197221827331 29.901383244109574 30.35464252399694 17.322002013620175 10 24.212004020505418 28.768436934091827 29.07424155627414 17.945317489128616 11 23.89330394245335 29.686633851553808 28.53246115788454 17.8876010481083 12 23.01510170623083 30.028761130501163 28.887963988022296 18.068173175245708 13 23.531063837085654 29.747190047680572 28.55460397681146 18.16714213842231 14 22.726300457966943 30.47334790650748 28.591590915834974 18.208760719690602 15 21.90153310946157 30.319449615632816 28.965285107763595 18.81373216714202 16 22.82466035635137 30.075804268676254 28.920842890940552 18.178692484031824 17 22.466953123525702 30.978118623333295 28.585517382275505 17.9694108708655 18 21.888461899886742 30.654183420627028 29.183173336102993 18.274181343383237 19 22.397847654279467 30.26093235343574 28.759186539926212 18.58203345235858 20 22.446624101128595 30.67797406502537 28.627508679611076 18.247893154234955 21 21.467043012115735 30.681027513218574 29.147124027797734 18.704805446867965 22 22.54504000464897 30.293058318630134 28.384445233011164 18.77745644370973 23 22.352130887087913 30.485530700605363 28.549577033913344 18.612761378393376 24 21.36525831116035 30.654420419588718 29.537816892353042 18.44250437689789 25 22.44699502467878 30.316941809513448 28.758259231050754 18.47780393475702 26 21.933599829330646 30.68334190177419 28.917613566814605 18.465444702080557 27 21.10826819018628 30.808045481760853 29.328964965390664 18.754721362662206 28 22.2162999273936 30.343390509126163 29.082011658993327 18.35829790448691 29 21.92831642502616 30.70452523375991 28.947059185603415 18.42009915561052 30 21.36470192583507 30.38129704547028 29.327503239399004 18.92649778929564 31 21.783474613992226 30.524535356432803 28.868176243088456 18.82381378648651 32 21.676401349172593 30.89725170377551 28.85439025113997 18.57195669591193 33 21.076656312274118 30.866488489977034 29.13069966642582 18.926155531323026 34 22.15298294550065 30.30899881860484 28.748333157144103 18.789685078750402 35 21.7378509991277 30.870551404243795 28.418155482235292 18.97344211439321 36 21.27408601725093 30.62621171955369 29.21579488802722 18.883907375168157 37 21.77103019534894 30.34489452368897 29.08769775842361 18.796377522538478 38 21.97367607601667 30.70928694189943 28.759288796567628 18.557748185516278 39 20.897956458520856 30.69584021602588 29.677160506038636 18.72904281941463 40 21.758295157240898 30.655408093918467 28.76661584345983 18.819680905380803 41 21.9941727874242 30.512993769098713 28.930632925791134 18.562200517685955 42 21.10715734082434 30.59902916942799 29.500848178518087 18.792965311229587 43 21.82813640823306 30.459151531937334 29.315059346768248 18.39765271306136 44 21.645717228385603 30.64867333978693 28.968839515831533 18.73676991599593 45 20.912733804038155 30.751827077914033 29.699925678186545 18.635513439861263 46 21.774461315836422 30.538176204729716 29.043476321684143 18.643886157749716 47 21.69856623872971 30.720251420965916 29.19998983807216 18.381192502232217 48 21.254020886919335 30.52285475223899 29.611701633146364 18.611422727695313 49 21.63824205165456 30.406167161156006 29.424853218168035 18.530737569021394 50 21.69921611070406 30.756590162145947 29.05479538666954 18.489398340480452 51 21.47180899293528 30.54209112922069 29.249878060949076 18.736221816894957 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 16.0 1 37.5 2 59.0 3 88.5 4 118.0 5 152.0 6 186.0 7 278.5 8 371.0 9 609.5 10 848.0 11 1457.5 12 2067.0 13 3107.5 14 4148.0 15 6280.5 16 8413.0 17 11701.0 18 14989.0 19 20389.0 20 25789.0 21 33350.0 22 40911.0 23 50715.5 24 60520.0 25 71831.5 26 95578.5 27 108014.0 28 118959.5 29 129905.0 30 138699.5 31 147494.0 32 151721.5 33 155949.0 34 156092.0 35 156235.0 36 152082.5 37 147930.0 38 139201.0 39 130472.0 40 120024.5 41 109577.0 42 98763.5 43 87950.0 44 77144.5 45 66339.0 46 57172.5 47 48006.0 48 40339.5 49 32673.0 50 26836.0 51 20999.0 52 16958.0 53 12917.0 54 10380.5 55 7844.0 56 6344.5 57 4845.0 58 3989.0 59 3133.0 60 2560.0 61 1987.0 62 1668.0 63 1349.0 64 1105.0 65 861.0 66 736.0 67 611.0 68 502.5 69 394.0 70 315.5 71 237.0 72 187.0 73 137.0 74 107.0 75 56.0 76 35.0 77 29.5 78 24.0 79 15.5 80 7.0 81 3.5 82 0.0 83 1.0 84 2.0 85 1.5 86 1.0 87 1.5 88 2.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 100.0 2 49.41041701698335 3 100.0 4 87.57913035737248 5 36.98954737435583 6 62.68607998100871 7 36.03621202979258 8 6.182059169724725E-4 9 36.50988140337689 10 11.616892847604824 11 24.05346492052345 12 12.216490766476424 13 13.493704190941552 14 0.0 15 13.493642370349857 16 12.43663389351032 17 24.653124659986744 18 0.003461953135045846 19 0.0 20 0.0 21 12.216490766476424 22 0.0013600530173394395 23 12.216490766476424 24 0.0 25 0.0 26 11.617263771155006 27 11.616831027013125 28 11.6196747742312 29 11.616831027013125 30 0.0 31 0.0 32 0.0 33 11.617140129971611 34 3.0910295848623624E-4 35 0.001421873609036687 36 0.002967388401467868 37 0.0010509500588532033 38 0.002720106034678879 39 0.0 40 12.217541716535276 41 6.182059169724726E-5 42 0.0 43 0.0035237737267430932 44 0.12042651262623766 45 0.1844726456245858 46 0.202771540766971 47 0.22978713933866804 48 0.25556632607642016 49 0.2556281466681174 50 0.26867229151623656 51 0.25179526998288804 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1617584.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.11958020726567 #Duplication Level Percentage of deduplicated Percentage of total 1 99.35592052372175 98.4811713341775 2 0.49301817079661386 0.9773550824782875 3 0.10138252052384249 0.3014697862403329 4 0.025408380313290627 0.1007387196159968 5 0.015282740197828252 0.07574093964127203 6 0.006383547544527796 0.037964073170803 7 0.0 0.0 8 0.0 0.0 9 0.0014290932603026301 0.01274860116344064 >10 0.0011750236418368288 0.012811463512372683 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 3.0910295848623624E-4 0.0 0.0 5 3.0910295848623624E-4 0.0 0.0 6 9.891294671559561E-4 0.0 0.0 7 0.03709235501834835 0.0 0.0 8 0.03752509916022908 0.0 0.0 9 0.0383287668522933 0.0 0.0 10 0.045561776080871225 0.0 0.0 11 0.06194423288064175 6.182059169724726E-5 0.0 12 0.07573022482912789 6.182059169724726E-5 0.0 13 0.08667246955954065 6.182059169724726E-5 0.0 14 0.09211268162889841 6.182059169724726E-5 0.0 15 0.09644012304770572 6.182059169724726E-5 0.0 16 0.10256036162573319 6.182059169724726E-5 0.0 17 0.1091133443456414 6.182059169724726E-5 0.0 18 0.11820097132513675 6.182059169724726E-5 0.0 19 0.12988506315591647 6.182059169724726E-5 0.0 20 0.1384163048101366 6.182059169724726E-5 0.0 21 0.14756575238132918 6.182059169724726E-5 0.0 22 0.15665337936082455 6.182059169724726E-5 0.0 23 0.16691559758256758 6.182059169724726E-5 0.0 24 0.1793415365137143 6.182059169724726E-5 0.0 25 0.1936220931957784 6.182059169724726E-5 0.0 26 0.20771718810275078 6.182059169724726E-5 0.0 27 0.22008130644220023 6.182059169724726E-5 0.0 28 0.2332490924737139 6.182059169724726E-5 0.0 29 0.24450044016261288 6.182059169724726E-5 0.0 30 0.25531904370963115 6.182059169724726E-5 0.0 31 0.26749770027398884 6.182059169724726E-5 0.0 32 0.2797381774300438 1.2364118339449451E-4 0.0 33 0.29383327233701617 1.2364118339449451E-4 0.0 34 0.3103393703201812 1.2364118339449451E-4 0.0 35 0.3272163918535297 1.2364118339449451E-4 0.0 36 0.34693716060495156 1.2364118339449451E-4 0.0 37 0.36715249408995143 1.2364118339449451E-4 0.0 38 0.39175708958545585 1.2364118339449451E-4 0.0 39 0.41728899395641894 1.2364118339449451E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGAGTAG 190 0.009609599 26.38833 4 TTTGTGG 420 4.766118E-5 20.890762 4 GTCGCCG 115 1.3042154E-9 17.650782 45 GGTCGCC 110 1.4386387E-8 17.03362 44 CTCGGTG 405 0.0 16.963524 44 TCTCGGT 415 0.0 16.178019 43 GGATTTG 620 9.335029E-4 14.151807 4 ATCTCGG 495 0.0 13.878386 42 TCGGTGG 565 0.0 12.988765 45 GATCTCG 625 0.0 11.989068 41 GTAACGG 195 0.0011872007 9.433331 23 AGATCTC 660 0.0 9.427755 40 TAGATCT 725 0.0 8.580389 39 TGGTCGC 185 8.9782727E-4 8.439821 43 AGAGTGT 780 0.0 8.355316 33 GTAGATC 795 0.0 8.041073 38 GTAGGGA 910 0.0 7.935937 25 TGTAGGG 955 0.0 7.93087 24 GAGTGTA 600 1.0913936E-11 7.684835 34 AGTGTAG 655 1.8189894E-12 7.5810466 35 >>END_MODULE