FastQCFastQC Report
Sun 19 Apr 2015
HCNNTADXX l01n02 dna2-frb rad27-frb.3420000000894f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCNNTADXX l01n02 dna2-frb rad27-frb.3420000000894f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1617584
Sequences flagged as poor quality0
Sequence length51
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGTAG1900.00960959926.388334
TTTGTGG4204.766118E-520.8907624
GTCGCCG1151.3042154E-917.65078245
GGTCGCC1101.4386387E-817.0336244
CTCGGTG4050.016.96352444
TCTCGGT4150.016.17801943
GGATTTG6209.335029E-414.1518074
ATCTCGG4950.013.87838642
TCGGTGG5650.012.98876545
GATCTCG6250.011.98906841
GTAACGG1950.00118720079.43333123
AGATCTC6600.09.42775540
TAGATCT7250.08.58038939
TGGTCGC1858.9782727E-48.43982143
AGAGTGT7800.08.35531633
GTAGATC7950.08.04107338
GTAGGGA9100.07.93593725
TGTAGGG9550.07.9308724
GAGTGTA6001.0913936E-117.68483534
AGTGTAG6551.8189894E-127.581046635
Produced by FastQC (version 0.11.2)