##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename HCNNTADXX l01n02 dna2-frb.342000000088c6.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3899464 Sequences flagged as poor quality 0 Sequence length 51 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 2.0 2.0 2.0 2.0 2.0 2.0 2 7.820885383221899 6.0 2.0 15.0 2.0 15.0 3 2.0 2.0 2.0 2.0 2.0 2.0 4 3.3521583992056345 2.0 2.0 2.0 2.0 6.0 5 9.50705096905626 15.0 2.0 15.0 2.0 15.0 6 7.101623710335574 2.0 2.0 15.0 2.0 15.0 7 12.13921913370658 15.0 2.0 15.0 2.0 27.0 8 17.247820213239564 15.0 15.0 22.0 6.0 27.0 9 12.670844249363503 15.0 2.0 15.0 2.0 27.0 10 17.534204700953772 15.0 15.0 27.0 2.0 33.0 11 17.830168453920848 22.0 6.0 27.0 2.0 33.0 12 18.679242326637713 15.0 6.0 27.0 2.0 33.0 13 18.39910613356092 15.0 15.0 27.0 2.0 33.0 14 21.12454250122581 22.0 15.0 27.0 6.0 33.0 15 19.8554088459337 22.0 6.0 27.0 2.0 33.0 16 20.58216154835639 22.0 15.0 33.0 2.0 33.0 17 19.236446598814606 22.0 6.0 33.0 2.0 33.0 18 22.05357351677051 22.0 15.0 33.0 6.0 33.0 19 22.832075639113476 27.0 15.0 33.0 6.0 33.0 20 24.152460697162482 27.0 15.0 33.0 6.0 33.0 21 21.05563251769987 27.0 6.0 33.0 2.0 33.0 22 22.68340007754912 27.0 15.0 33.0 6.0 33.0 23 21.611298116869396 27.0 15.0 33.0 2.0 33.0 24 23.656233266931046 27.0 15.0 33.0 6.0 33.0 25 23.556295429320542 27.0 15.0 33.0 6.0 33.0 26 22.017134149719038 27.0 15.0 33.0 2.0 33.0 27 21.858841112522132 27.0 15.0 33.0 2.0 33.0 28 22.305917428651732 27.0 15.0 33.0 2.0 33.0 29 21.87385753529203 27.0 15.0 33.0 2.0 33.0 30 22.353382413582995 27.0 15.0 33.0 6.0 33.0 31 22.38568659692717 27.0 15.0 33.0 6.0 33.0 32 23.763140523928417 27.0 15.0 33.0 6.0 33.0 33 21.628655374174503 27.0 15.0 33.0 2.0 33.0 34 22.656799755043256 27.0 15.0 33.0 6.0 33.0 35 21.779792556105146 27.0 15.0 27.0 6.0 33.0 36 22.65876823071068 27.0 15.0 27.0 6.0 33.0 37 23.16763765481615 27.0 15.0 33.0 6.0 33.0 38 24.182543293129516 27.0 22.0 33.0 6.0 33.0 39 24.109202957124364 27.0 22.0 33.0 6.0 33.0 40 20.483672371382323 27.0 6.0 27.0 2.0 33.0 41 22.287672100575875 27.0 15.0 27.0 6.0 33.0 42 23.521042892048754 27.0 15.0 33.0 6.0 33.0 43 23.849536756846582 27.0 15.0 33.0 6.0 33.0 44 24.073962729236634 27.0 22.0 33.0 6.0 33.0 45 24.6186086087729 27.0 22.0 33.0 6.0 33.0 46 24.823074914911384 27.0 22.0 33.0 6.0 33.0 47 25.071337496640563 27.0 22.0 33.0 6.0 33.0 48 25.04503131712461 27.0 22.0 33.0 6.0 33.0 49 25.247546585889754 27.0 22.0 33.0 15.0 33.0 50 25.43455100495863 27.0 22.0 33.0 15.0 33.0 51 24.043783709761136 27.0 22.0 33.0 6.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2201 1 0.0 2201 2 -5.693594254619937 2201 3 0.0 2201 4 9.612635988495686 2201 5 3.627175855426392 2201 6 11.354425441788013 2201 7 11.137333004319753 2201 8 5.5786639772909545 2201 9 10.07497666764164 2201 10 4.773113720668448 2201 11 8.267927947409472 2201 12 7.293712096937561 2201 13 8.017773535755286 2201 14 6.554599999463708 2201 15 3.20000345528117 2201 16 3.998590409661187 2201 17 6.735176030622 2201 18 2.50509578151442 2201 19 2.5393321990452797 2201 20 1.5708140242436208 2201 21 5.288998550770884 2201 22 4.495988503387004 2201 23 2.697776752214466 2201 24 2.4931388259488543 2201 25 -0.15469055305360868 2201 26 3.8437325213522264 2201 27 4.336255114099085 2201 28 2.912122013854635 2201 29 4.302875343432916 2201 30 2.2448797938533502 2201 31 2.775397295526954 2201 32 1.4121882778582417 2201 33 2.0191633545176195 2201 34 -0.40521454924648737 2201 35 -3.500623624678621 2201 36 -2.274808976618349 2201 37 -0.6035956558390723 2201 38 -0.3238851301874952 2201 39 0.4216083979244978 2201 40 3.1612697344747183 2201 41 0.23443151567471787 2201 42 -1.7582907038058941 2201 43 -2.287694277538467 2201 44 -1.5148496685974777 2201 45 -3.101592653326314 2201 46 -2.0408388771905166 2201 47 -0.7929168113246732 2201 48 -0.6947710635261224 2201 49 -0.7753877779552205 2201 50 -1.2170584952235082 2201 51 -1.3887931026307143 1101 1 0.0 1101 2 6.474844365728686 1101 3 0.0 1101 4 -1.3732337126422411 1101 5 4.549586111066876 1101 6 7.787736947695942 1101 7 8.178983193751087 1101 8 3.7328178181745137 1101 9 -10.450136916619126 1101 10 -4.5842280440625345 1101 11 0.0718455953917676 1101 12 3.268288853244666 1101 13 6.848660699477659 1101 14 1.817928200087671 1101 15 2.6131129624577483 1101 16 5.468610297357667 1101 17 6.043635044533236 1101 18 3.2844924356940695 1101 19 0.44588248475916004 1101 20 0.8772834259976356 1101 21 0.12120911326913486 1101 22 1.769693004156366 1101 23 3.944969508219689 1101 24 1.0957530985291974 1101 25 0.6295106931600039 1101 26 0.727319390905997 1101 27 -0.4088895539530988 1101 28 2.1478654842073013 1101 29 2.569294209897137 1101 30 2.3876249621425742 1101 31 1.698596240114071 1101 32 0.5065765008412093 1101 33 2.188507821522929 1101 34 2.732043504507434 1101 35 1.6374959242009197 1101 36 0.7787222444144426 1101 37 1.3033751372168538 1101 38 0.8659922921670677 1101 39 0.7979276788408143 1101 40 1.034589288997779 1101 41 -0.6169426029184741 1101 42 1.300996997619663 1101 43 0.4858403404289504 1101 44 -1.4075938642654044 1101 45 -2.470153186021644 1101 46 -2.274366389019278 1101 47 -1.0983128539818914 1101 48 -0.3308002882118366 1101 49 -1.6487141549979896 1101 50 -1.1381325778990181 1101 51 -1.3180406618240461 1102 1 0.0 1102 2 6.108923561082525 1102 3 0.0 1102 4 -1.3732337126422411 1102 5 4.327206779577114 1102 6 6.510161237306038 1102 7 7.783327308434181 1102 8 6.765126866752556 1102 9 -10.450136916619126 1102 10 -3.005742133829038 1102 11 -15.601022909161742 1102 12 -6.2818666103765946 1102 13 -2.611887343969853 1102 14 -1.0504233655685873 1102 15 -1.5726570348604803 1102 16 -18.68629142102532 1102 17 -17.14027726436362 1102 18 -8.056147819732235 1102 19 -5.2691691745406395 1102 20 -3.3877617253171977 1102 21 0.01298778850489768 1102 22 1.1346911352893443 1102 23 4.827550674402406 1102 24 2.51564882671488 1102 25 0.7415407727423009 1102 26 4.648416550469065 1102 27 4.023785220352529 1102 28 4.338064513943124 1102 29 1.1486017026139663 1102 30 0.7567753722583497 1102 31 -0.9481501024058687 1102 32 0.03167570188072588 1102 33 2.4302215185903577 1102 34 -0.020728349074918384 1102 35 0.6574610033592378 1102 36 1.9964162837240131 1102 37 0.9948464413903082 1102 38 0.7656371633842802 1102 39 -1.4728162810916992 1102 40 2.0133210324065303 1102 41 0.3424867112236427 1102 42 -0.2013471755942966 1102 43 0.8914255013265056 1102 44 1.1450348329430433 1102 45 0.6253325515465704 1102 46 1.000161151671989 1102 47 0.0730681113323719 1102 48 1.4017387634998961 1102 49 -0.06745751399266808 1102 50 0.41937638445834935 1102 51 0.5503660897472038 1103 1 0.0 1103 2 5.056630243364358 1103 3 0.0 1103 4 -1.3732337126422411 1103 5 4.813398144012389 1103 6 -5.130464725357999 1103 7 1.391900044081888 1103 8 -0.5229005289107711 1103 9 9.041504115880539 1103 10 7.067640668955512 1103 11 7.094290337174641 1103 12 6.863990897480875 1103 13 -16.430998920582507 1103 14 -7.442508664353904 1103 15 -18.00134288292899 1103 16 -6.963235955693541 1103 17 -0.4308254215695264 1103 18 0.12795649298276146 1103 19 1.163770050579192 1103 20 1.0623017626657933 1103 21 2.5994534737266086 1103 22 0.46122592039481347 1103 23 1.8453191492906456 1103 24 1.8445905510698353 1103 25 0.36072065717043245 1103 26 2.1497632633157018 1103 27 2.081421824171837 1103 28 1.1267254773140571 1103 29 3.7984888520329747 1103 30 0.225498267738935 1103 31 -0.6766396335535596 1103 32 -0.1289434623732042 1103 33 2.167285148988448 1103 34 0.23224855477458206 1103 35 1.4018966633986416 1103 36 0.7933368297674583 1103 37 1.5333415204649548 1103 38 -1.1014421495002935 1103 39 -0.12334945651346985 1103 40 4.456407944946498 1103 41 1.8910011365409893 1103 42 1.1637012566466076 1103 43 1.745812683481276 1103 44 1.443081303321371 1103 45 1.5858274257221794 1103 46 1.081897774113429 1103 47 -0.21421510106454278 1103 48 -0.48683254680003074 1103 49 -0.21967526041165897 1103 50 0.109845829315379 1103 51 0.6403596455041729 2103 1 0.0 2103 2 5.13397884830418 2103 3 0.0 2103 4 -1.3732337126422411 2103 5 4.832304743337943 2103 6 -5.130464725357999 2103 7 -10.001343999556699 2103 8 -3.5608076788542693 2103 9 2.9320183695923223 2103 10 3.102877417656245 2103 11 5.54090276786534 2103 12 5.701152826228608 2103 13 5.847452217463015 2103 14 3.7838658263428755 2103 15 4.860731600050329 2103 16 5.395431847905439 2103 17 6.934225715755588 2103 18 2.3491816544904154 2103 19 0.535338101554732 2103 20 1.9112377449813742 2103 21 4.674915400474756 2103 22 1.185624642040004 2103 23 2.970836605457663 2103 24 1.6882645551996056 2103 25 1.045570114619565 2103 26 5.171033813979147 2103 27 4.749189448153704 2103 28 2.8089041299810944 2103 29 3.1930660417198453 2103 30 0.754278861825572 2103 31 4.0439664351913756 2103 32 -0.4012293082387224 2103 33 3.694173838743417 2103 34 1.088057633810397 2103 35 -0.2506192666200917 2103 36 0.6905688381941317 2103 37 -2.3324071391724814 2103 38 -0.12031358855128715 2103 39 -1.2114329947578462 2103 40 1.2733681886517232 2103 41 0.943260143923009 2103 42 -0.6260293948465936 2103 43 1.174042668868239 2103 44 0.5278159244836651 2103 45 1.004996637130386 2103 46 0.7614070928533643 2103 47 1.0906735854361251 2103 48 0.7713070919172154 2103 49 0.900409792899719 2103 50 1.1792335300225893 2103 51 0.9272208534208382 2102 1 0.0 2102 2 -5.693594254619937 2102 3 0.0 2102 4 -1.3732337126422411 2102 5 -7.383223877806904 2102 6 -5.130464725357999 2102 7 1.5124884480831877 2102 8 -4.290124083457938 2102 9 7.727203658560619 2102 10 3.4468605861733543 2102 11 5.480252489400307 2102 12 4.082156471344714 2102 13 8.098170351064883 2102 14 3.9653822265737517 2102 15 6.491623669118525 2102 16 3.6046979506533283 2102 17 7.860406543076206 2102 18 4.646260449958582 2102 19 2.2480112599346462 2102 20 1.4305476124057677 2102 21 2.576878539019056 2102 22 -1.5839741576981687 2102 23 0.9974288460775789 2102 24 -1.9729523912900717 2102 25 1.1279739711051775 2102 26 2.656654075769925 2102 27 2.4198957387373063 2102 28 3.580442511835219 2102 29 1.2933289808088375 2102 30 -0.47671385475086225 2102 31 1.079744106529553 2102 32 -0.19484210776789723 2102 33 2.6550029017902794 2102 34 1.659182237156518 2102 35 1.9315850925139486 2102 36 -0.6861769896883736 2102 37 2.1538271710939583 2102 38 -0.48985858760664414 2102 39 -0.40195758510688506 2102 40 2.1242933572620935 2102 41 3.0816062904603356 2102 42 2.668773117010055 2102 43 3.1404518855682575 2102 44 0.7924260485885384 2102 45 1.7376549243934178 2102 46 1.5602660282986207 2102 47 1.3246622111580848 2102 48 1.0070540038193236 2102 49 1.493514412676884 2102 50 1.0568580129325085 2102 51 1.102211094668501 2101 1 0.0 2101 2 -5.693594254619937 2101 3 0.0 2101 4 -1.3732337126422411 2101 5 -7.383223877806904 2101 6 -5.130464725357999 2101 7 -10.001343999556699 2101 8 -4.202594620540303 2101 9 -10.450136916619126 2101 10 -15.312439195008263 2101 11 -15.601022909161742 2101 12 -4.49308194087161 2101 13 -4.018648190079272 2101 14 -2.423090730511319 2101 15 1.490373632893018 2101 16 3.337102472545805 2101 17 7.137936616309734 2101 18 2.823148148118694 2101 19 3.48388843477089 2101 20 1.5548628886839992 2101 21 3.671146553952699 2101 22 1.3579761824135232 2101 23 2.2081003716204 2101 24 2.0365123862447376 2101 25 1.4769960438531378 2101 26 -19.788504567556114 2101 27 -19.66528713090946 2101 28 -20.11401278669557 2101 29 -19.662028162048305 2101 30 -8.300658256159936 2101 31 -8.526015365170277 2101 32 -3.582961139765981 2101 33 -19.43561135266424 2101 34 -9.59973595278463 2101 35 -4.264420850060599 2101 36 -3.793033606261673 2101 37 -1.6387657930383064 2101 38 -0.7923485132670081 2101 39 0.05273235982138047 2101 40 4.295967568021059 2101 41 2.165364939075701 2101 42 1.3115884736743233 2101 43 -2.2072337736769327 2101 44 -0.47693509612933127 2101 45 0.6578480290441426 2101 46 -0.4115487199509644 2101 47 -0.5644816754564239 2101 48 -0.947685521998018 2101 49 -0.1835078790020681 2101 50 -0.8162929231598923 2101 51 -0.3874169361201858 2104 1 0.0 2104 2 -5.693594254619937 2104 3 0.0 2104 4 -1.3732337126422411 2104 5 -7.383223877806904 2104 6 -5.130464725357999 2104 7 -10.001343999556699 2104 8 -3.500181750454754 2104 9 1.5747079381822662 2104 10 4.511916979446298 2104 11 4.746826681081949 2104 12 -16.4343525939882 2104 13 -5.750522349129223 2104 14 -5.2057534920342015 2104 15 0.9181545979886891 2104 16 3.8450943985954105 2104 17 -17.14027726436362 2104 18 -7.6799871430267235 2104 19 -5.147053356103271 2104 20 -5.0192857336609755 2104 21 -18.94558941971805 2104 22 -8.821225229982858 2104 23 -19.491981907282845 2104 24 -9.700955852417053 2104 25 -5.227621699596995 2104 26 0.5915849517640588 2104 27 2.4636293393480706 2104 28 3.199888655560141 2104 29 3.3563730315426312 2104 30 2.4083148530920226 2104 31 0.5531010237677734 2104 32 2.35753553756561 2104 33 4.281256768511188 2104 34 4.31414692085713 2104 35 2.387225057886557 2104 36 2.4949753764683322 2104 37 -1.410621682116215 2104 38 1.1962185135613908 2104 39 1.9372878808832432 2104 40 -18.359217114760412 2104 41 -8.041208133979945 2104 42 -3.859392570703875 2104 43 -2.9426450284578074 2104 44 -0.5089794803443972 2104 45 -0.03991372848872743 2104 46 0.32302193922333444 2104 47 0.1815225339009494 2104 48 -0.7200104387004131 2104 49 0.5008183807830093 2104 50 0.4061702395535889 2104 51 -0.12590698276575907 >>END_MODULE >>Per sequence quality scores fail #Quality Count 5 16.0 6 312.0 7 3106.0 8 12822.0 9 31299.0 10 57729.0 11 88120.0 12 119536.0 13 152840.0 14 184818.0 15 216394.0 16 245382.0 17 269705.0 18 287900.0 19 301476.0 20 302085.0 21 272397.0 22 242625.0 23 231841.0 24 228464.0 25 217701.0 26 188986.0 27 145432.0 28 74141.0 29 20780.0 30 3557.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 NaN NaN NaN NaN 2 38.75068774860421 28.270751968539447 25.72510500380329 7.253455279053056 3 NaN NaN NaN NaN 4 40.158098977546025 23.13526153230546 25.82519496650869 10.88144452363982 5 38.990122271829 26.490748416057087 23.847086692294145 10.672042619819768 6 14.526351164762183 34.46837991073611 37.82841252559226 13.17685639890944 7 22.24759982631909 30.834995095122466 30.092790634096133 16.824614444462313 8 22.160971419046277 32.07444639628665 28.68189103591532 17.082691148751746 9 21.851780991775566 32.023744942474174 29.040410156060798 17.084063909689462 10 22.7589573440757 29.247134661558306 29.08663106812721 18.907276926238787 11 22.600370186362237 30.761885925357902 28.199607672659134 18.43813621562073 12 22.796761242445076 30.181900071486822 28.773926773866837 18.24741191220126 13 22.866507922073957 29.68733199785668 28.69013792059591 18.75602215947345 14 21.96076178674813 31.146331906128637 28.238650235006656 18.654256072116578 15 21.588399790644587 30.469595332096986 28.35465541810806 19.587349459150367 16 22.635923466686126 29.766328588755485 28.44833169085549 19.1494162537029 17 22.25564903764669 31.74972712419408 27.412120431448848 18.582503406710373 18 22.114272994262656 30.792406146233077 28.185211742222577 18.90810911728169 19 21.940579525801496 30.15291332347215 28.27155219281419 19.634954957912164 20 22.505580254106718 30.577228049803768 28.08891170683971 18.828279989249804 21 20.999901949288873 30.510035359355857 29.16682790238106 19.32323478897421 22 22.124828595908458 29.801808529544587 28.089883318275504 19.983479556271448 23 22.386141157527923 30.34989556289982 28.052948547816598 19.211014731755657 24 21.32264844604284 30.24387454275767 29.295051832764706 19.13842517843478 25 22.59584080979397 30.03199672980445 28.01938524324259 19.352777217158987 26 21.688448870906306 30.722212978417616 28.409789904103416 19.179548246572658 27 21.197042468640653 31.050513817012092 28.36616016898523 19.386283545362023 28 22.371325612952244 30.089368305312675 28.341687303984138 19.197618777750947 29 22.303870201164194 30.70060633045285 28.113940382674897 18.881583085708055 30 21.726715121214156 30.128104851387192 28.439051335799657 19.706128691598995 31 21.4157894520888 30.79592477325089 28.186412286406544 19.60187348825377 32 21.689134711847576 31.189414750334915 27.950456780726785 19.170993757090717 33 21.170404700147603 30.995337652082867 28.3079944019359 19.52626324583363 34 22.378426507109204 30.024577812472113 28.107051965250502 19.48994371516818 35 21.66925808500724 31.228629774409406 27.639214045000156 19.462898095583203 36 21.397091766484763 30.437614746894543 28.52937020416003 19.63592328246066 37 21.521220381927126 30.080357795424206 28.718263030413382 19.680158792235286 38 22.247793415984653 30.872121605815117 27.628070605559437 19.252014372640794 39 20.761596978456527 30.724504701158928 29.245763007428714 19.26813531295583 40 21.563572258415352 30.862101221339888 27.87463816165609 19.699688358588674 41 22.207935351081765 30.715416639526172 27.93446890878793 19.142179100604135 42 21.180680216563097 30.732505800797238 28.711920407522673 19.374893575116992 43 21.39493231217745 30.33060195334018 29.076194833424456 19.198270901057914 44 21.415544845502303 30.639986874750146 28.19882391393821 19.74564436580934 45 20.880478874780945 30.570016476157612 29.371699084164053 19.177805564897383 46 21.440254211482635 30.417598276065426 28.347196229381353 19.79495128307058 47 22.00561262887764 30.611138071287275 28.33580772916264 19.047441570672447 48 21.623787536646752 30.286034268166674 28.77468280619175 19.31549538899483 49 21.409916837768087 30.2139140548954 29.023480102614574 19.352689004721938 50 21.99807921502378 30.66704208113324 28.07076819828055 19.264110505562435 51 21.891966657874022 30.356765478528203 28.31003282073748 19.441235042860296 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4.0 1 6.0 2 8.0 3 21.5 4 35.0 5 69.0 6 103.0 7 266.0 8 429.0 9 848.0 10 1267.0 11 2279.5 12 3292.0 13 5369.0 14 7446.0 15 11408.0 16 15370.0 17 22506.0 18 29642.0 19 41042.0 20 52442.0 21 69587.5 22 86733.0 23 109304.0 24 131875.0 25 159547.0 26 216658.5 27 246098.0 28 274439.5 29 302781.0 30 325528.0 31 348275.0 32 363167.0 33 378059.0 34 381476.5 35 384894.0 36 375424.0 37 365954.0 38 346026.5 39 326099.0 40 301278.5 41 276458.0 42 249797.5 43 223137.0 44 197216.5 45 171296.0 46 148722.5 47 126149.0 48 106106.0 49 86063.0 50 71592.5 51 57122.0 52 46181.5 53 35241.0 54 28175.5 55 21110.0 56 16956.0 57 12802.0 58 10325.5 59 7849.0 60 6424.0 61 4999.0 62 4102.5 63 3206.0 64 2684.5 65 2163.0 66 1801.0 67 1439.0 68 1190.0 69 941.0 70 813.5 71 686.0 72 529.0 73 372.0 74 294.0 75 159.5 76 103.0 77 77.5 78 52.0 79 37.5 80 23.0 81 14.5 82 6.0 83 3.5 84 1.0 85 1.0 86 1.0 87 1.5 88 2.0 89 1.0 90 0.0 91 1.0 92 2.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 100.0 2 48.82265870386289 3 100.0 4 87.69507809278403 5 36.51773679664692 6 62.76157441125242 7 36.21379758859166 8 3.590237017190055E-4 9 36.465729648997915 10 11.532277256566543 11 24.019506270605394 12 11.859758161634522 13 13.422126733315142 14 0.0 15 13.42202415511465 16 12.487280303139098 17 24.34703846477362 18 0.0025644550122786106 19 0.0 20 0.0 21 11.859758161634522 22 0.0013591611565076636 23 11.859758161634522 24 0.0 25 7.693365036835832E-5 26 11.53250805751765 27 11.532225967466298 28 11.53509815708005 29 11.532225967466298 30 5.128910024557221E-5 31 0.0 32 0.0 33 11.532354190216912 34 3.077346014734333E-4 35 0.0014104502567532358 36 0.003102990564857119 37 7.43691953560797E-4 38 0.0028465450636292575 39 0.0 40 11.860630076338696 41 5.128910024557221E-5 42 0.0 43 0.002359298611296322 44 0.12004213912476175 45 0.18394835803074472 46 0.20261759052013303 47 0.22957001269918123 48 0.25554794197356356 49 0.25536843012270405 50 0.26744701323053627 51 0.2525988187094431 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 3899464.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.38841668684975 #Duplication Level Percentage of deduplicated Percentage of total 1 98.45648548063582 95.88521233510933 2 1.117637698177953 2.176899317101722 3 0.22289985214194327 0.6512359103951032 4 0.07723687447286401 0.3008790765901275 5 0.03608087559232479 0.17569296733058565 6 0.030098251422518114 0.17587326306490572 7 0.01660176417763087 0.11317736692275517 8 0.01454144053060814 0.11329342956975288 9 0.00431299187001881 0.037803190446394845 >10 0.023455131145030735 0.3299729990421788 >50 6.496398332866359E-4 0.039960144427140964 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0010257820049114443 0.0 0.0 5 0.001436094806876022 0.0 0.0 6 0.003590237017190055 0.0 0.0 7 0.12342721974096953 0.0 0.0 8 0.1260429638534937 0.0 0.0 9 0.12971013452105212 0.0 0.0 10 0.15309796423303304 0.0 0.0 11 0.21269589871838795 0.0 0.0 12 0.25067547745023416 0.0 0.0 13 0.2825772978029801 0.0 0.0 14 0.2978870942262834 0.0 0.0 15 0.3095040754319055 0.0 0.0 16 0.3253524074077873 0.0 0.0 17 0.3389183744227412 0.0 0.0 18 0.35945965907109284 0.0 0.0 19 0.38536065469510683 0.0 0.0 20 0.39892662171006066 0.0 0.0 21 0.41410819538275007 0.0 0.0 22 0.4282126979502824 0.0 0.0 23 0.44585614843475924 0.0 0.0 24 0.4633713761686222 0.0 0.0 25 0.48919543814226774 0.0 0.0 26 0.5090443199373043 0.0 0.0 27 0.5263800358203076 0.0 0.0 28 0.5415872540431198 0.0 0.0 29 0.5558456239113888 2.5644550122786104E-5 0.0 30 0.5697706146280617 5.128910024557221E-5 0.0 31 0.5844905863985409 5.128910024557221E-5 0.0 32 0.5996208709709847 5.128910024557221E-5 0.0 33 0.615058890144902 5.128910024557221E-5 0.0 34 0.6332152316318346 5.128910024557221E-5 0.0 35 0.6511664167177849 5.128910024557221E-5 0.0 36 0.6719897914174872 5.128910024557221E-5 0.0 37 0.6933260571196451 5.128910024557221E-5 0.0 38 0.7166369531812578 5.128910024557221E-5 0.0 39 0.741307010399378 5.128910024557221E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCTCGG 325 0.0 26.450945 7 CTCGGTG 2620 0.0 23.96558 44 TCGGTGG 2805 0.0 22.77543 45 TCTCGGT 2800 0.0 22.08713 43 TAGATCG 1580 0.0 21.687092 6 ATCTCGG 3010 0.0 20.701149 42 TAGCTCG 330 0.0056236107 19.228735 6 GCAGACC 415 9.750432E-4 18.348383 5 GGTCGCC 380 0.0 18.130674 44 GTCGCCG 380 0.0 18.130442 45 GCTCGGA 340 2.0008883E-11 18.009443 8 GATCTCG 3650 0.0 16.380018 41 AGATCGG 5085 0.0 16.060429 7 TCGGACG 270 7.6397555E-10 15.970279 10 GATCGGA 5255 0.0 15.070129 8 AGACCGG 335 1.18275755E-4 14.663636 7 AAGATCG 1500 1.6370905E-11 14.383094 6 AGATCTC 4100 0.0 14.246378 40 GCACACG 325 0.0 14.173529 18 CGGACGA 395 3.6379788E-12 14.035362 11 >>END_MODULE