FastQCFastQC Report
Sun 19 Apr 2015
HCNNTADXX l01n02 dna2-frb.342000000088c6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCNNTADXX l01n02 dna2-frb.342000000088c6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3899464
Sequences flagged as poor quality0
Sequence length51
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTCGG3250.026.4509457
CTCGGTG26200.023.9655844
TCGGTGG28050.022.7754345
TCTCGGT28000.022.0871343
TAGATCG15800.021.6870926
ATCTCGG30100.020.70114942
TAGCTCG3300.005623610719.2287356
GCAGACC4159.750432E-418.3483835
GGTCGCC3800.018.13067444
GTCGCCG3800.018.13044245
GCTCGGA3402.0008883E-1118.0094438
GATCTCG36500.016.38001841
AGATCGG50850.016.0604297
TCGGACG2707.6397555E-1015.97027910
GATCGGA52550.015.0701298
AGACCGG3351.18275755E-414.6636367
AAGATCG15001.6370905E-1114.3830946
AGATCTC41000.014.24637840
GCACACG3250.014.17352918
CGGACGA3953.6379788E-1214.03536211
Produced by FastQC (version 0.11.2)