##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename HCNNTADXX l01n01 wt-cdc9deg.34100000008879.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2454829 Sequences flagged as poor quality 0 Sequence length 51 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.489114720414335 33.0 33.0 33.0 27.0 33.0 2 31.671806467986162 33.0 33.0 33.0 27.0 33.0 3 31.681572932371257 33.0 33.0 33.0 27.0 33.0 4 35.345001220044246 37.0 37.0 37.0 33.0 37.0 5 35.41295870302982 37.0 37.0 37.0 33.0 37.0 6 35.05228062728605 37.0 33.0 37.0 33.0 37.0 7 35.009362362918154 37.0 33.0 37.0 33.0 37.0 8 34.973700408460225 37.0 33.0 37.0 33.0 37.0 9 35.050757099578014 37.0 33.0 37.0 33.0 37.0 10 34.940938044971766 37.0 33.0 37.0 33.0 37.0 11 35.01688386441581 37.0 33.0 37.0 33.0 37.0 12 34.832978590362096 37.0 33.0 37.0 27.0 37.0 13 34.27715576115485 37.0 33.0 37.0 27.0 37.0 14 35.69611162325359 37.0 33.0 40.0 27.0 40.0 15 35.798751766416316 37.0 33.0 40.0 27.0 40.0 16 35.59940672038663 37.0 33.0 40.0 27.0 40.0 17 35.832174461031705 37.0 33.0 40.0 27.0 40.0 18 35.75947000789057 37.0 33.0 40.0 27.0 40.0 19 35.90162043873524 37.0 33.0 40.0 27.0 40.0 20 35.83803841326626 37.0 33.0 40.0 27.0 40.0 21 35.83147054234735 37.0 33.0 40.0 27.0 40.0 22 35.825767090090594 37.0 33.0 40.0 27.0 40.0 23 35.77183298714493 37.0 33.0 40.0 27.0 40.0 24 35.68022579169465 37.0 33.0 40.0 27.0 40.0 25 35.744353272671944 37.0 33.0 40.0 27.0 40.0 26 35.56649159676702 37.0 33.0 40.0 27.0 40.0 27 35.47196566441084 37.0 33.0 40.0 27.0 40.0 28 35.283127663882084 37.0 33.0 40.0 27.0 40.0 29 35.34815133762881 37.0 33.0 40.0 27.0 40.0 30 35.24316439149122 37.0 33.0 40.0 27.0 40.0 31 35.24213906549092 37.0 33.0 40.0 27.0 40.0 32 35.20904103707427 37.0 33.0 40.0 27.0 40.0 33 35.12303830531577 37.0 33.0 40.0 27.0 40.0 34 35.13871679045669 37.0 33.0 40.0 27.0 40.0 35 35.13793384386448 37.0 33.0 40.0 27.0 40.0 36 35.08888480623294 37.0 33.0 40.0 27.0 40.0 37 35.12597211455462 37.0 33.0 40.0 27.0 40.0 38 35.05801992725359 37.0 33.0 40.0 27.0 40.0 39 35.02141574830671 37.0 33.0 40.0 27.0 40.0 40 35.0430893557148 37.0 33.0 40.0 27.0 40.0 41 34.89097692751715 37.0 33.0 40.0 27.0 40.0 42 34.55737527950012 37.0 33.0 40.0 27.0 40.0 43 34.71081529507758 37.0 33.0 40.0 27.0 40.0 44 34.66786729340414 37.0 33.0 40.0 27.0 40.0 45 34.69394365147226 37.0 33.0 40.0 27.0 40.0 46 34.51797457175225 37.0 33.0 40.0 27.0 40.0 47 34.620333228913296 37.0 33.0 40.0 27.0 40.0 48 34.481255924547085 37.0 33.0 40.0 27.0 40.0 49 34.47969614176792 37.0 33.0 40.0 27.0 40.0 50 34.43739136208673 37.0 33.0 40.0 27.0 40.0 51 33.34313306548032 37.0 33.0 37.0 22.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.0825203162272139 2201 2 0.05317607460884588 2201 3 -0.43922390371718123 2201 4 -0.2582640863089196 2201 5 -0.053644813829720306 2201 6 -0.21161247750100642 2201 7 0.01978147075898562 2201 8 0.03602410939382139 2201 9 -0.13333538634566366 2201 10 0.11113756014039922 2201 11 0.07236751828323662 2201 12 0.07210715912852095 2201 13 0.7861965760567529 2201 14 -0.045725645067363985 2201 15 -0.0033934221775595574 2201 16 0.3220811053623578 2201 17 0.0548183166653331 2201 18 0.1455306549702513 2201 19 0.01085042624247734 2201 20 0.04493013143274993 2201 21 0.02592628885702908 2201 22 -0.04354044936033574 2201 23 -0.28516499166640585 2201 24 -0.029777044443243028 2201 25 -0.11468857874071148 2201 26 -0.22714455642325504 2201 27 -0.27197548222199686 2201 28 -0.029157446792623887 2201 29 -0.155266377503267 2201 30 -0.25805066799657794 2201 31 -0.29105324911675723 2201 32 -0.2499611132519206 2201 33 -0.4558316524624004 2201 34 -0.29412124466755785 2201 35 -0.36604384607831975 2201 36 -0.15423042943410792 2201 37 -0.20231531702879835 2201 38 -0.22643192682414792 2201 39 -0.14941718526469572 2201 40 -0.11530192037204756 2201 41 -0.07395263270861108 2201 42 0.17069846447535042 2201 43 0.0832626623570647 2201 44 -0.06227551824278521 2201 45 -0.2086968935409672 2201 46 -0.3001281510983276 2201 47 -0.11635892928866554 2201 48 -0.1626600620339147 2201 49 -0.16240940618824595 2201 50 -0.1794171410527312 2201 51 -0.3640347992798709 1101 1 0.1011088149398951 1101 2 0.0427207568658865 1101 3 0.12428773942472304 1101 4 -0.61689546846371 1101 5 -0.19052070753384243 1101 6 -0.2587395148402578 1101 7 -0.15381834824911778 1101 8 -0.16081592130028355 1101 9 -0.12317033596961124 1101 10 -0.14206219982965962 1101 11 -0.08756613846042427 1101 12 -0.20175331179839873 1101 13 0.22737883074626808 1101 14 -0.21105096426352787 1101 15 -0.13905132323198188 1101 16 0.08619714648067855 1101 17 -0.006715011863178688 1101 18 -0.3260806651279964 1101 19 -0.2395968906466166 1101 20 -0.12082901163797999 1101 21 -0.28274403213566757 1101 22 -0.23230193919945918 1101 23 -0.11951292575162142 1101 24 -0.16807062568240383 1101 25 -0.09496087049789992 1101 26 -0.07679670387121718 1101 27 -0.18414096485879838 1101 28 -0.16217756187859322 1101 29 -0.12201393610000366 1101 30 -0.37064064030816013 1101 31 -0.22448172928194055 1101 32 -0.06804399340503409 1101 33 -0.04954687352022091 1101 34 -0.04212061177361193 1101 35 -0.20775501828915566 1101 36 -0.6762045808223007 1101 37 -0.2971879537816733 1101 38 -0.24312448727968672 1101 39 -0.1384929587826207 1101 40 -0.21877030570068 1101 41 -0.13397960161465505 1101 42 0.06607883378709545 1101 43 -0.013350661911111672 1101 44 -0.2920184086240383 1101 45 -0.18782400866017923 1101 46 -0.17418010738091283 1101 47 -0.1900623883121213 1101 48 -0.4882517561241997 1101 49 -0.36798593353348963 1101 50 -0.4053303264827335 1101 51 -0.3541700911702037 1102 1 0.00657608299087542 1102 2 -0.030455965055079304 1102 3 0.06136957953091127 1102 4 0.07983877852040422 1102 5 -0.09219657369260403 1102 6 -0.08378928447815781 1102 7 -0.2903971596721959 1102 8 -0.06565589440592845 1102 9 0.01019596653596011 1102 10 -0.1931876742512415 1102 11 -0.13808572266400887 1102 12 -0.5373826993572308 1102 13 0.5601246145394754 1102 14 0.1606146568436344 1102 15 0.07615658066158915 1102 16 -0.7100716568740353 1102 17 -0.476330280616736 1102 18 -0.5339014968599329 1102 19 -0.361178288291903 1102 20 -0.24044728172988528 1102 21 -0.2070249464558671 1102 22 -0.32919196917993787 1102 23 -0.10781864667920615 1102 24 -0.5398108158141355 1102 25 -0.302395764999261 1102 26 -0.2516173732078144 1102 27 -0.06148170254310514 1102 28 -0.31558521056037137 1102 29 -0.13238060079552838 1102 30 -0.23883804344751525 1102 31 -0.26362036546993295 1102 32 -0.17951609578383199 1102 33 -0.336544844979322 1102 34 -0.3051117184976846 1102 35 -0.27440812934356984 1102 36 -0.16499854319441454 1102 37 -0.09757150384241697 1102 38 -0.1740664356212065 1102 39 -0.19013397865450798 1102 40 -0.1573764743045558 1102 41 -0.08434274896730898 1102 42 0.16381256140731892 1102 43 -0.0974183399263282 1102 44 -0.09404215115605297 1102 45 -0.34918727836171826 1102 46 -0.33187732305835027 1102 47 -0.26889325112131957 1102 48 -0.2205804708001864 1102 49 -0.26947450511023163 1102 50 -0.23241318268063793 1102 51 -0.6003201523470096 1103 1 -0.22678655268423498 1103 2 -0.02910026095457141 1103 3 0.01623106142706021 1103 4 0.18803532603105566 1103 5 0.047426471886296895 1103 6 0.19600689623241863 1103 7 0.1053976287545737 1103 8 0.09292885494476621 1103 9 0.05680461291937888 1103 10 0.19491142444950071 1103 11 0.0979770082499769 1103 12 0.1621027253198335 1103 13 0.7130619526765258 1103 14 0.45906293325290193 1103 15 0.33335806356681275 1103 16 0.5698493382822818 1103 17 0.22925310706614255 1103 18 0.21608093016653385 1103 19 0.14231020845194564 1103 20 0.10317071937752331 1103 21 0.07407156867374454 1103 22 0.19183051920704486 1103 23 0.04992114629863664 1103 24 0.18882993780978552 1103 25 0.15014563298505124 1103 26 0.1430871391810129 1103 27 0.1776710877185721 1103 28 0.2471561712433683 1103 29 0.15768390563904688 1103 30 0.14721058136201037 1103 31 0.03664335600375779 1103 32 -0.1576127136269463 1103 33 -0.027367233175901617 1103 34 0.03936615125241616 1103 35 0.11722587330747558 1103 36 0.15896885261403781 1103 37 -0.10710229363687063 1103 38 0.21319107534051795 1103 39 -0.08190078382810384 1103 40 0.03275663851410826 1103 41 0.06890672272392351 1103 42 0.2755061286633591 1103 43 0.1829373680797275 1103 44 0.06555358406920675 1103 45 0.11470080986349274 1103 46 -0.09372192283327507 1103 47 -0.03625383965338358 1103 48 0.046232018735473446 1103 49 0.010756183627599114 1103 50 -0.04796483831007947 1103 51 0.03649037232459307 2103 1 -0.02237991643313819 2103 2 -0.06230440816139193 2103 3 0.06909364633923687 2103 4 0.19264196362855301 2103 5 0.003725223176900272 2103 6 0.1314637109118877 2103 7 0.1191818748245339 2103 8 0.07632858920680974 2103 9 0.0898887687944736 2103 10 0.13554520176457885 2103 11 0.04653816747442363 2103 12 0.2012023390318447 2103 13 0.8637415857115229 2103 14 0.508980224519064 2103 15 0.26896428206328693 2103 16 -0.26644859233709894 2103 17 0.14120992345328887 2103 18 0.1637044197154296 2103 19 0.1528926542334048 2103 20 0.21620010092723874 2103 21 0.1582052647212464 2103 22 0.17916731456076462 2103 23 0.22084221934490955 2103 24 0.19008123833281587 2103 25 0.16476767243140245 2103 26 0.20480981552310595 2103 27 0.08896903166374415 2103 28 0.4354596420745622 2103 29 0.3731144266602442 2103 30 0.3642750652919915 2103 31 0.30666616126644897 2103 32 0.07387399298984576 2103 33 0.1480530577847219 2103 34 0.18466320570607309 2103 35 0.24161286675251148 2103 36 0.3429832502708763 2103 37 0.20326359227080104 2103 38 0.23107192872566173 2103 39 0.31456408359638033 2103 40 0.1504548081567023 2103 41 0.05818888395930344 2103 42 0.5711194475353452 2103 43 0.4455122276767369 2103 44 0.27800199278139814 2103 45 0.30828112259074913 2103 46 0.20715128358207835 2103 47 0.15693275719245747 2103 48 0.39200204395714877 2103 49 0.3480779208391169 2103 50 0.37877576891831666 2103 51 0.2105510102737682 2102 1 -0.16366945262744892 2102 2 -0.08074402462562347 2102 3 -0.13382980257986787 2102 4 0.05920597332669786 2102 5 -0.006335353621295781 2102 6 -0.014204736098434978 2102 7 -0.014401635747724129 2102 8 0.0407167154908592 2102 9 -0.006829698278977503 2102 10 -0.17760809963490942 2102 11 -0.13393995148703652 2102 12 -0.00372282853606265 2102 13 0.519426597187433 2102 14 0.17651155392744755 2102 15 0.08297531664322833 2102 16 -0.26727917598540785 2102 17 -0.09894209396713194 2102 18 -0.06293524557007402 2102 19 -0.031004756391752153 2102 20 0.14229911476943613 2102 21 0.1733313277887376 2102 22 0.16816535741039473 2102 23 0.10267093340279843 2102 24 -0.03714786690918004 2102 25 0.06618073892133225 2102 26 -0.025351858542094874 2102 27 0.003235961357709982 2102 28 -0.6574105419977485 2102 29 -0.3046300088588012 2102 30 -0.12588884595716365 2102 31 -0.18926424206087944 2102 32 0.06022554692619053 2102 33 0.09299943863061344 2102 34 -0.08481753722629293 2102 35 -0.010102341293425354 2102 36 0.004624340371293556 2102 37 0.05885614060048283 2102 38 0.12309883467057148 2102 39 0.10106623036505624 2102 40 0.0550894558215731 2102 41 -0.14822642573772526 2102 42 0.32550579807416824 2102 43 0.10256903825293051 2102 44 0.18980981850891254 2102 45 0.14377074705166137 2102 46 0.24968941247019671 2102 47 0.1413818690927826 2102 48 -0.013915140751137756 2102 49 0.03474247135604713 2102 50 0.012535141826170104 2102 51 0.04149081971817736 2101 1 0.14593667228161067 2101 2 0.10021816339044776 2101 3 0.1882678270393896 2101 4 0.12768176752990712 2101 5 0.20589605204011008 2101 6 0.17054012948529618 2101 7 0.08378396696247137 2101 8 0.15793515041937667 2101 9 0.1834644313572511 2101 10 0.026514203520704882 2101 11 0.19661670579235846 2101 12 0.2535918440839495 2101 13 -4.459739179110205 2101 14 -1.4843167165235656 2101 15 -0.7502101723150432 2101 16 -0.07728789045640383 2101 17 -0.06322457987830887 2101 18 0.13694440573780042 2101 19 0.16426515629068916 2101 20 -0.10497966670466496 2101 21 -0.012146096507294146 2101 22 -0.03010445628810743 2101 23 0.022991307123156446 2101 24 0.23133175301391873 2101 25 0.1260363992130351 2101 26 -0.024853214441037608 2101 27 0.17257690682441762 2101 28 0.2552644257371952 2101 29 0.004027386557702073 2101 30 0.1889649324428575 2101 31 0.35793118348163233 2101 32 0.25260032896369466 2101 33 0.3140930674853166 2101 34 0.2323840188654458 2101 35 0.2018004475811992 2101 36 0.28867750914568546 2101 37 0.19894195472290477 2101 38 0.17202468717480457 2101 39 0.020184076348527924 2101 40 0.14798642691236097 2101 41 0.00569326948325255 2101 42 -2.1790378864481212 2101 43 -0.89108875033196 2101 44 -0.2939922349290143 2101 45 -0.1794389283242026 2101 46 0.1109669633755459 2101 47 0.14402350933973196 2101 48 0.15692496238425946 2101 49 0.06693189356007423 2101 50 0.33191130718253703 2101 51 0.3842799263120824 2104 1 0.07669403530519858 2104 2 0.006489663931485978 2104 3 0.11380385253574588 2104 4 0.22775574573599044 2104 5 0.08564970157416951 2104 6 0.07033527628825453 2104 7 0.13047220236846613 2104 8 -0.17746160374942832 2104 9 -0.07701835901281129 2104 10 0.04474958384062688 2104 11 -0.05390758718852595 2104 12 0.053854772127529316 2104 13 0.7898090221922232 2104 14 0.43592395731143085 2104 15 0.1312006747897101 2104 16 0.34295972552760645 2104 17 0.21993061914057677 2104 18 0.2606569969680237 2104 19 0.16146149011178323 2104 20 -0.040344106434410776 2104 21 0.0703806250580854 2104 22 0.09597562284965022 2104 23 0.11607095792771815 2104 24 0.16456342369239962 2104 25 0.004914770687079795 2104 26 0.25786675178130736 2104 27 0.07514516205947075 2104 28 0.22645052217418282 2104 29 0.17946520440064262 2104 30 0.29296761861251497 2104 31 0.26717888517766397 2104 32 0.2684340471880162 2104 33 0.31414504023724277 2104 34 0.2697577363412407 2104 35 0.29767014736329145 2104 36 0.20017960104895138 2104 37 0.2431153806955706 2104 38 -0.09576367618655013 2104 39 0.12413051621998505 2104 40 0.10516137097251033 2104 41 0.30771253286181377 2104 42 0.6063166525054697 2104 43 0.1875764558029971 2104 44 0.20896291759236618 2104 45 0.3583944293811072 2104 46 0.3320998449430306 2104 47 0.16923027275050373 2104 48 0.29024840463255686 2104 49 0.33936137544917244 2104 50 0.14190327059912988 2104 51 0.6457129141684348 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 3.0 10 11.0 11 6.0 12 21.0 13 29.0 14 12.0 15 18.0 16 43.0 17 91.0 18 184.0 19 491.0 20 1038.0 21 2094.0 22 3926.0 23 6786.0 24 11036.0 25 17738.0 26 26827.0 27 38566.0 28 54229.0 29 73106.0 30 94912.0 31 121041.0 32 151558.0 33 185594.0 34 226162.0 35 272888.0 36 323441.0 37 385674.0 38 447268.0 39 10034.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 23.188417626592933 26.502453806205427 13.294466947587516 37.014661619614124 2 16.78532394720773 32.07722411622154 25.21882379587336 25.918628140697376 3 15.02850096686979 28.675765195864965 30.58412622630741 25.71160761095783 4 15.100155652389638 28.684360499244548 31.892567669682897 24.322916178682917 5 16.040384075632154 29.25283186731133 31.43404286001184 23.272741197044684 6 17.479099641777985 29.35668736095115 32.28463189662934 20.879581100641527 7 17.341248616502412 32.91850471051141 29.403636668786298 20.336610004199883 8 16.9969069128644 30.94969140416705 30.789354370508086 21.26404731246046 9 18.220576667458303 29.709360611268647 30.603231426710376 21.466831294562677 10 18.546342739148024 30.19631917335179 29.959479866011034 21.297858221489154 11 18.438799606815788 30.025146354389655 30.673908447390836 20.86214559140372 12 19.93108277603043 30.1164765448021 29.18724685100266 20.76519382816481 13 18.629525722565603 30.165156106596424 29.96583468746703 21.23948348337094 14 18.54789070847705 30.514834230816078 30.09896004976314 20.838315010943738 15 18.919077459163145 30.485463549599583 30.253390358350824 20.342068632886445 16 18.971178847895313 30.056024268900195 30.273106599278403 20.69969028392609 17 18.49362976240185 30.169811897220722 30.83533114032854 20.501227200048884 18 19.165868575946803 30.098740557850135 30.56354098900244 20.171849877200625 19 18.86368950579716 29.711767289033585 30.489705567246833 20.934837637922424 20 18.94698164312056 29.9098633754123 30.614637516503183 20.528517464963954 21 19.41416383142362 29.784350436040828 30.34928076075763 20.452204971777928 22 19.20263125412712 29.74931959806078 30.095417180795657 20.952631967016444 23 19.047008003858537 29.808221878951418 30.316691760197102 20.82807835699295 24 19.534906457822 29.837968856616186 30.296625963683027 20.330498721878786 25 18.895002462493316 29.815722398586626 30.553289047831843 20.73598609108822 26 18.714510166961126 30.010300608257438 30.846899060558446 20.42829016422299 27 19.175454870247457 30.036000164581495 30.689885693264912 20.098659271906143 28 19.305724626699064 29.719447825867313 30.527444931695257 20.447382615738363 29 19.15779062411272 29.825906407330205 30.6868217704777 20.329481198079378 30 19.677992194293473 29.840039248572392 30.356032471347973 20.125936085786165 31 19.446270817598688 29.434237123254526 30.211294400217696 20.90819765892909 32 19.20783012407799 29.678954973201776 30.609774130722855 20.503440771997376 33 19.489228803692004 29.845286527102964 30.335304868161927 20.3301798010431 34 19.02759825633476 29.59424057643119 30.451245280221144 20.926915887012903 35 18.937740219888028 29.923748901008018 30.8037980511073 20.334712827996654 36 19.47105073306532 29.879555765391398 30.626002870261026 20.023390631282258 37 18.887941970370864 29.774241408276048 30.72827086794316 20.609545753409925 38 18.862015290399036 29.914129503577357 30.7661127865524 20.457742419471202 39 19.31482803893876 30.12303504643297 30.535813288827857 20.026323625800412 40 19.252298225253163 29.684063533549587 30.416578914457993 20.647059326739257 41 19.097542028385682 30.05850916703363 30.53817597885637 20.305772825724315 42 19.752536734737937 29.849899931930086 30.4219560710746 19.975607262257373 43 19.049392035046026 29.64805287863228 30.35653399890583 20.946021087415865 44 18.896202061366825 29.93959945070412 30.72765409839338 20.436544389535676 45 19.1344896121074 29.765128243148503 30.807318961931767 20.293063182812325 46 19.102335701775864 29.556712458909402 30.71207677550693 20.628875063807804 47 18.878622846721306 30.01845769293911 30.780881398629546 20.322038061710042 48 19.191925775799735 29.953049730621554 30.821456578639157 20.033567914939553 49 18.989190089904 29.6745537902316 30.570415973553956 20.765840146310442 50 19.08843305743916 29.788871299809433 30.748621275978632 20.37407436677277 51 19.439726727760476 29.869454249916995 30.383041477658118 20.307777544664408 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 28.0 1 34.0 2 40.0 3 77.5 4 115.0 5 174.0 6 233.0 7 268.5 8 304.0 9 399.5 10 495.0 11 666.0 12 837.0 13 1185.0 14 1533.0 15 2184.0 16 2835.0 17 3838.0 18 4841.0 19 6573.0 20 8305.0 21 11450.5 22 14596.0 23 19728.0 24 24860.0 25 33093.5 26 53189.0 27 65051.0 28 80323.0 29 95595.0 30 114108.5 31 132622.0 32 153702.0 33 174782.0 34 193036.0 35 211290.0 36 225790.5 37 240291.0 38 245368.0 39 250445.0 40 248080.0 41 245715.0 42 233695.5 43 221676.0 44 206361.0 45 191046.0 46 172913.0 47 154780.0 48 137469.5 49 120159.0 50 104465.5 51 88772.0 52 75176.0 53 61580.0 54 50573.0 55 39566.0 56 31530.0 57 23494.0 58 18778.5 59 14063.0 60 11286.0 61 8509.0 62 6922.0 63 5335.0 64 4269.5 65 3204.0 66 2682.5 67 2161.0 68 1745.5 69 1330.0 70 1066.5 71 803.0 72 762.5 73 722.0 74 608.5 75 452.5 76 410.0 77 346.0 78 282.0 79 225.5 80 169.0 81 122.0 82 75.0 83 56.0 84 37.0 85 26.5 86 16.0 87 10.5 88 5.0 89 2.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18551190327309966 2 0.0 3 0.0 4 0.0 5 0.0 6 0.008391623204712017 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0021997458886138304 18 4.888324196919622E-4 19 2.851522448203113E-4 20 0.0 21 5.295684546662924E-4 22 0.0012220810492299056 23 5.295684546662924E-4 24 0.0021997458886138304 25 0.0 26 0.024645301159469765 27 0.004806852126970962 28 0.009817384428813575 29 0.0 30 0.029615097426338048 31 0.0022404819235881603 32 8.147206994866037E-5 33 0.009247079939172953 34 0.0 35 0.012505962737119368 36 0.0 37 0.0033810909028694058 38 0.003503299007792396 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0020368017487165093 45 0.0 46 0.007821318715071395 47 0.0010591369093325847 48 0.004236547637330339 49 0.013117003261734322 50 9.369288044095943E-4 51 0.0054586286865602445 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 2454829.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.5163907110496 #Duplication Level Percentage of deduplicated Percentage of total 1 86.37950630070674 68.68586572434567 2 10.309456252429856 16.395415027733712 3 1.9579470534222363 4.670666486744125 4 0.46800206650621234 1.4885534069554638 5 0.21114238130298593 0.8394640043674823 6 0.11573946509163202 0.5521910716148458 7 0.10162570184884745 0.5656636310148312 8 0.07521272865476057 0.4784515774524878 9 0.05462924295450422 0.39095282043172896 >10 0.3065169403090101 4.685399267254346 >50 0.01643437467180691 0.827903055092776 >100 0.0037246548921262158 0.3937414983403045 >500 6.28372093242493E-5 0.02573242865226793 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.03576623870746191 0.0 0.0 2 0.03613286302223088 0.0 0.0 3 0.03662169544192284 0.0 0.0 4 0.039758370134946264 0.0 0.0 5 0.04110265928909916 0.0 0.0 6 0.04977943473863149 0.0 0.0 7 0.19883258670970563 0.0 0.0 8 0.2013582208781141 0.0 0.0 9 0.20596139283021342 0.0 0.0 10 0.22661456256219883 0.0 0.0 11 0.28274881875682584 0.0 0.0 12 0.3164375196805969 0.0 0.0 13 0.34609335314190925 0.0 0.0 14 0.3591696203686693 0.0 0.0 15 0.37342723260968486 0.0 0.0 16 0.38964017452946825 0.0 0.0 17 0.4050383957497651 0.0 0.0 18 0.4216994340542661 0.0 0.0 19 0.4474853441930171 0.0 0.0 20 0.4603579312449054 0.0 0.0 21 0.4775892740390471 0.0 0.0 22 0.4927838150844723 0.0 0.0 23 0.5108298785781006 0.0 0.0 24 0.5263503079033204 0.0 0.0 25 0.552950938741558 0.0 0.0 26 0.5767815192015411 0.0 0.0 27 0.604644967123983 0.0 0.0 28 0.621428213533407 0.0 0.0 29 0.6404112058314448 0.0 0.0 30 0.6598015584792261 0.0 0.0 31 0.6837950830791065 0.0 0.0 32 0.7072590392243208 0.0 0.0 33 0.7275455846415372 0.0 0.0 34 0.7480765462685995 0.0 0.0 35 0.7695037006650972 0.0 0.0 36 0.7897087740123651 0.0 0.0 37 0.8123987454930669 0.0 0.0 38 0.8344776764491539 0.0 0.0 39 0.8599784343430846 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CACGATC 715 0.0 20.138884 43 CGATCTC 805 0.0 17.328354 45 TCACGAT 950 0.0 17.051111 42 ACGATCT 990 0.0 15.453796 44 ATCACGA 1215 0.0 13.517038 41 CCCCGGG 185 1.5336609E-6 13.3752775 7 CATCACG 1260 0.0 13.0342865 40 GCCCCGG 235 1.3070166E-7 12.444685 6 GTCACAT 1295 0.0 12.334054 36 AGTCACA 1425 0.0 11.841049 35 TCACATC 1445 0.0 11.676445 37 CCCCGGA 255 3.6844722E-7 11.490397 1 TGCCCCG 235 1.7613638E-6 11.487402 5 GTGCCCC 255 3.7760765E-7 11.468631 4 CAGTCAC 1505 0.0 11.361114 34 TCCAGTC 1615 0.0 10.727035 32 CCAGTCA 1595 0.0 10.720484 33 CCCGGGT 210 8.201142E-5 10.711803 8 ACATCAC 1565 0.0 10.492776 39 CACATCA 1695 0.0 9.821133 38 >>END_MODULE