FastQCFastQC Report
Sun 19 Apr 2015
HCNNTADXX l01n01 wt-cdc9deg.34100000008879.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCNNTADXX l01n01 wt-cdc9deg.34100000008879.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2454829
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGATC7150.020.13888443
CGATCTC8050.017.32835445
TCACGAT9500.017.05111142
ACGATCT9900.015.45379644
ATCACGA12150.013.51703841
CCCCGGG1851.5336609E-613.37527757
CATCACG12600.013.034286540
GCCCCGG2351.3070166E-712.4446856
GTCACAT12950.012.33405436
AGTCACA14250.011.84104935
TCACATC14450.011.67644537
CCCCGGA2553.6844722E-711.4903971
TGCCCCG2351.7613638E-611.4874025
GTGCCCC2553.7760765E-711.4686314
CAGTCAC15050.011.36111434
TCCAGTC16150.010.72703532
CCAGTCA15950.010.72048433
CCCGGGT2108.201142E-510.7118038
ACATCAC15650.010.49277639
CACATCA16950.09.82113338
Produced by FastQC (version 0.11.2)