##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	HCNNTADXX l01n01 rnh201-frbrad27-frb.341000000088e3.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1573759
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.426783262240278	33.0	33.0	33.0	27.0	33.0
2	31.610425738629612	33.0	33.0	33.0	27.0	33.0
3	31.587473050193836	33.0	33.0	33.0	27.0	33.0
4	35.19076491381463	37.0	37.0	37.0	33.0	37.0
5	35.25225145654449	37.0	37.0	37.0	33.0	37.0
6	34.895415371731	37.0	33.0	37.0	27.0	37.0
7	34.84759483504145	37.0	33.0	37.0	27.0	37.0
8	34.84812795351766	37.0	33.0	37.0	27.0	37.0
9	34.92286747843857	37.0	33.0	37.0	33.0	37.0
10	34.81965218308521	37.0	33.0	37.0	27.0	37.0
11	34.92556738357017	37.0	33.0	37.0	33.0	37.0
12	34.70683694263226	37.0	33.0	37.0	27.0	37.0
13	34.09802199701479	37.0	33.0	37.0	27.0	37.0
14	35.49806037646171	37.0	33.0	40.0	27.0	40.0
15	35.66129312048414	37.0	33.0	40.0	27.0	40.0
16	35.405021353333005	37.0	33.0	40.0	27.0	40.0
17	35.62812603454532	37.0	33.0	40.0	27.0	40.0
18	35.57247710735888	37.0	33.0	40.0	27.0	40.0
19	35.73267889174899	37.0	33.0	40.0	27.0	40.0
20	35.69362526282614	37.0	33.0	40.0	27.0	40.0
21	35.69371549265167	37.0	33.0	40.0	27.0	40.0
22	35.6987022790656	37.0	33.0	40.0	27.0	40.0
23	35.650991670262094	37.0	33.0	40.0	27.0	40.0
24	35.53266351455337	37.0	33.0	40.0	27.0	40.0
25	35.52934407364787	37.0	33.0	40.0	27.0	40.0
26	35.39912782071461	37.0	33.0	40.0	27.0	40.0
27	35.298843088427134	37.0	33.0	40.0	27.0	40.0
28	35.08339332769503	37.0	33.0	40.0	27.0	40.0
29	35.12590746105344	37.0	33.0	40.0	27.0	40.0
30	35.071074414824636	37.0	33.0	40.0	27.0	40.0
31	35.06978514499361	37.0	33.0	40.0	27.0	40.0
32	35.02925797406083	37.0	33.0	40.0	27.0	40.0
33	34.946145502583306	37.0	33.0	40.0	27.0	40.0
34	34.971725022700426	37.0	33.0	40.0	27.0	40.0
35	34.95258295584013	37.0	33.0	40.0	27.0	40.0
36	34.88928419154394	37.0	33.0	40.0	27.0	40.0
37	34.83042575133804	37.0	33.0	40.0	27.0	40.0
38	34.822767653751306	37.0	33.0	40.0	27.0	40.0
39	34.79859177930039	37.0	33.0	40.0	27.0	40.0
40	34.83126895541185	37.0	33.0	40.0	27.0	40.0
41	34.65588250805873	37.0	33.0	40.0	27.0	40.0
42	34.25089991542542	37.0	33.0	40.0	22.0	40.0
43	34.37915081025748	37.0	33.0	40.0	27.0	40.0
44	34.26489316343862	37.0	33.0	40.0	22.0	40.0
45	34.30338571534777	37.0	33.0	40.0	27.0	40.0
46	34.15692872923999	37.0	33.0	40.0	22.0	40.0
47	34.1940131875338	37.0	33.0	40.0	22.0	40.0
48	33.72736295709826	37.0	33.0	37.0	22.0	40.0
49	33.721965688520285	37.0	33.0	37.0	22.0	40.0
50	33.80878647874293	37.0	33.0	37.0	22.0	40.0
51	32.80021655158128	37.0	33.0	37.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
2201	1	0.060842402321377165
2201	2	0.08717877309821986
2201	3	-0.4268334286809825
2201	4	-0.2072167999537271
2201	5	-0.05035485481641899
2201	6	-0.20460711924756225
2201	7	0.033246692777446185
2201	8	-0.004088078189901978
2201	9	-0.2064320532234305
2201	10	0.10465893505720203
2201	11	0.02870119714560815
2201	12	0.07351252586823875
2201	13	0.7825521391137755
2201	14	0.009262566050360022
2201	15	-0.00483517654744503
2201	16	0.2929356277049564
2201	17	0.04838954014319086
2201	18	0.11810922093861365
2201	19	-0.04057865776331937
2201	20	0.05251752313857594
2201	21	-0.004506185899536774
2201	22	-0.09559103103077149
2201	23	-0.3181527109600282
2201	24	-0.04538308158817017
2201	25	-0.11029428797404961
2201	26	-0.2530371878364761
2201	27	-0.2880302116033491
2201	28	-0.10370714561635452
2201	29	-0.1329058829756704
2201	30	-0.3271633437236616
2201	31	-0.306251163312794
2201	32	-0.25137992444188484
2201	33	-0.4282878217478512
2201	34	-0.2887305303558634
2201	35	-0.2919740535185298
2201	36	-0.1383913248266282
2201	37	-0.2157435932383791
2201	38	-0.1837543855142556
2201	39	-0.12388956905773796
2201	40	-0.21465352246211467
2201	41	-0.03883814068628055
2201	42	0.15525640526686146
2201	43	0.03547586024649263
2201	44	-0.19009563761394332
2201	45	-0.22850947823650358
2201	46	-0.31267998889514104
2201	47	-0.16474361790385217
2201	48	-0.35841949035404497
2201	49	-0.2810032152902906
2201	50	-0.3143565153040413
2201	51	-0.4826806072709857
1101	1	0.08151979347814375
1101	2	0.04629674405439843
1101	3	0.12932693851388066
1101	4	-0.5607690403492285
1101	5	-0.14029446566707549
1101	6	-0.17760928006689625
1101	7	-0.14815176471480385
1101	8	-0.13895361808850026
1101	9	-0.0772627619205224
1101	10	-0.1163860322898671
1101	11	-0.05910812536635035
1101	12	-0.18139500342577008
1101	13	0.20740323828650276
1101	14	-0.16246981429039664
1101	15	-0.11251994323392722
1101	16	0.08220715180700466
1101	17	-0.04762990740641726
1101	18	-0.327398442044867
1101	19	-0.2457704824935476
1101	20	-0.1426452829960212
1101	21	-0.28327682248408337
1101	22	-0.19811900893986234
1101	23	-0.059230377342124996
1101	24	-0.14009009586293075
1101	25	-0.055705557042749376
1101	26	-0.07410142404144437
1101	27	-0.08830873867399447
1101	28	-0.061089669822436576
1101	29	-0.09483949530637403
1101	30	-0.3030128857341623
1101	31	-0.1902129207121348
1101	32	-0.07582564721644047
1101	33	-0.09087841850803358
1101	34	0.05316889392707935
1101	35	-0.22626145845918444
1101	36	-0.6446029191744955
1101	37	-0.25469385540094436
1101	38	-0.23265958464458691
1101	39	-0.09969723242625861
1101	40	-0.12720200835948248
1101	41	-0.014119898298680766
1101	42	0.10039421107882873
1101	43	-0.0036551753394178377
1101	44	-0.22458791375679255
1101	45	-0.19399846155405953
1101	46	-0.15940974781258177
1101	47	-0.14462425659762346
1101	48	-0.5029588336776456
1101	49	-0.30977910515551343
1101	50	-0.3663516003455882
1101	51	-0.27948136710468674
1102	1	-0.04191884370106891
1102	2	-0.11267502297397058
1102	3	0.033201595137335005
1102	4	-0.01183669080882055
1102	5	-0.18297434066826668
1102	6	-0.1616379765709013
1102	7	-0.4609103730428501
1102	8	-0.17742396150589457
1102	9	-0.05942961117459333
1102	10	-0.3995951531988027
1102	11	-0.242929137041763
1102	12	-0.67728432398156
1102	13	0.5208668340367737
1102	14	0.11391750007267376
1102	15	0.011996318009330764
1102	16	-0.7961582357284271
1102	17	-0.5643157280825335
1102	18	-0.6557808049745404
1102	19	-0.41173654885280797
1102	20	-0.3132708188554929
1102	21	-0.23153576021193345
1102	22	-0.3698676647130199
1102	23	-0.1909365940614478
1102	24	-0.541138599047585
1102	25	-0.26189491445611424
1102	26	-0.31045661073308395
1102	27	-0.1714529986389195
1102	28	-0.38437289883626136
1102	29	-0.2138984163713502
1102	30	-0.3139623767924391
1102	31	-0.31878276997995414
1102	32	-0.26372453662398243
1102	33	-0.40674540480651444
1102	34	-0.3738608128676404
1102	35	-0.3573505857883035
1102	36	-0.2068518874901315
1102	37	-0.19208265905543698
1102	38	-0.28082360739796997
1102	39	-0.23828891199930524
1102	40	-0.24709031005068027
1102	41	-0.2119887403681915
1102	42	0.10656697885931266
1102	43	-0.1823468967880899
1102	44	-0.13676187453113897
1102	45	-0.3765024851840195
1102	46	-0.303008678120932
1102	47	-0.3718929586591173
1102	48	-0.33432440227468874
1102	49	-0.35881624415121394
1102	50	-0.3633067675635431
1102	51	-0.6664498581793339
1103	1	-0.22973147290762697
1103	2	-0.0785786784555711
1103	3	-0.03176042320087902
1103	4	0.12541938747312997
1103	5	0.03152435814566701
1103	6	0.14976503439797284
1103	7	0.11231964908947134
1103	8	0.03783193842033228
1103	9	0.043713503078464555
1103	10	0.23390775318875967
1103	11	0.11568544434924632
1103	12	0.20042972315299323
1103	13	0.8184598424924587
1103	14	0.45119100857294825
1103	15	0.2938709177223373
1103	16	0.5491972191192005
1103	17	0.2034326930639665
1103	18	0.22271841269240866
1103	19	0.10049784299471298
1103	20	0.13933722823041705
1103	21	0.035477348933660835
1103	22	0.14421676240029058
1103	23	-0.015223779202798937
1103	24	0.06921495063664196
1103	25	0.024337239837798563
1103	26	0.09663262387765315
1103	27	0.1750593362806967
1103	28	0.2512240658736289
1103	29	0.14856459508395403
1103	30	0.16241197565468468
1103	31	-0.009823637761613213
1103	32	-0.17511254949802435
1103	33	-0.059167799799581644
1103	34	-0.04799842241453689
1103	35	0.1431107069663966
1103	36	0.1536796125625699
1103	37	-0.17816523775490367
1103	38	0.18488183001944236
1103	39	-0.1605424365515944
1103	40	-0.027832756280481874
1103	41	-0.027659722702864542
1103	42	0.1998000006106082
1103	43	0.12691665622003967
1103	44	0.07636753875960522
1103	45	0.1284148631528126
1103	46	-0.11126614271326929
1103	47	-0.07864980305996738
1103	48	0.09326682262377517
1103	49	0.0062981872588494525
1103	50	-0.006925280887529084
1103	51	0.003326548601421564
2103	1	0.041221313210836996
2103	2	0.011148339347435865
2103	3	0.10383456806080105
2103	4	0.2743743988915526
2103	5	0.084516061880052
2103	6	0.21504379669548968
2103	7	0.22415424415716245
2103	8	0.2152127209415582
2103	9	0.15130503289926622
2103	10	0.24273920521550707
2103	11	0.11511449759348835
2103	12	0.29948542284174806
2103	13	1.0145554187941315
2103	14	0.5783952841283266
2103	15	0.3842277054501153
2103	16	-0.10631212291957581
2103	17	0.21469002161186523
2103	18	0.309580962555863
2103	19	0.3160536435092567
2103	20	0.364579988808849
2103	21	0.289866611320015
2103	22	0.2555169612799162
2103	23	0.34886964915907015
2103	24	0.3183611846909997
2103	25	0.26689049398415676
2103	26	0.322021879213537
2103	27	0.13987621803913441
2103	28	0.5363413617921253
2103	29	0.4895602737015565
2103	30	0.503424066878047
2103	31	0.44958797537642425
2103	32	0.23867478030284417
2103	33	0.2910139349801639
2103	34	0.3826247464458916
2103	35	0.40005576895391215
2103	36	0.48588340695176413
2103	37	0.36607963752248196
2103	38	0.3759795433722033
2103	39	0.44609463494816026
2103	40	0.34535950051638054
2103	41	0.1749558426953186
2103	42	0.6842163173956806
2103	43	0.5581637324440223
2103	44	0.41561348274213117
2103	45	0.4438803986973454
2103	46	0.35286496562525116
2103	47	0.37747537114533714
2103	48	0.6204712541720312
2103	49	0.4963894479149715
2103	50	0.6014123669658815
2103	51	0.40227108694548264
2102	1	-0.1515603471988527
2102	2	-0.0679398508956588
2102	3	-0.13194941788048098
2102	4	0.05390169454630467
2102	5	0.03597766879694575
2102	6	0.018583504638520765
2102	7	0.009934791661372344
2102	8	0.12107683747969133
2102	9	-0.0012537601750608474
2102	10	-0.11409208037940743
2102	11	-0.0919285855402805
2102	12	0.022604427459782528
2102	13	0.5748201441902552
2102	14	0.22045190785242852
2102	15	0.15278636699216008
2102	16	-0.2887637368878089
2102	17	-0.017799993197293418
2102	18	0.018400246120492625
2102	19	0.016625521929874765
2102	20	0.19022375331530839
2102	21	0.20847775945654945
2102	22	0.19684274880799535
2102	23	0.19342675935025966
2102	24	0.023714841138477993
2102	25	0.1329739292371528
2102	26	0.06831847613700148
2102	27	0.14173125715629453
2102	28	-0.5784935583742978
2102	29	-0.26323131459169247
2102	30	-0.08996438541509377
2102	31	-0.14156337512645223
2102	32	0.14282408299455085
2102	33	0.24453777082251804
2102	34	-0.027365069986799995
2102	35	0.03211636999536438
2102	36	0.03716101152023299
2102	37	0.051470073540507144
2102	38	0.1673605780326426
2102	39	0.17899708099238154
2102	40	0.10440302321983808
2102	41	-0.1220161197504197
2102	42	0.29609171213969887
2102	43	0.19196343794872917
2102	44	0.2603090854275365
2102	45	0.15831847071283534
2102	46	0.2935008991061565
2102	47	0.1696677790736274
2102	48	0.05847005654567994
2102	49	0.10021656925108857
2102	50	0.008399617659186731
2102	51	0.05839290108207962
2101	1	0.08839816618156249
2101	2	0.05035582331002075
2101	3	0.1526460796565665
2101	4	0.12745341808858512
2101	5	0.15442951873978927
2101	6	0.11217041042324638
2101	7	0.10466011013501486
2101	8	0.11201195158896127
2101	9	0.17474054432218367
2101	10	-0.029441747050057643
2101	11	0.15843550441221765
2101	12	0.20215628803357788
2101	13	-4.62947860321572
2101	14	-1.628487116176352
2101	15	-0.8769999793901988
2101	16	-0.1050879233095614
2101	17	-0.06400878215751504
2101	18	0.07097603090512195
2101	19	0.12317258705760281
2101	20	-0.23429596880827575
2101	21	-0.07243110341799763
2101	22	-0.08039258743839639
2101	23	-0.0761261558174553
2101	24	0.10475417378186336
2101	25	-0.0188496390936308
2101	26	-0.10729877142387778
2101	27	0.033165809788009426
2101	28	0.1748879647615169
2101	29	-0.08892907493022051
2101	30	0.10698875401903507
2101	31	0.21844167023017036
2101	32	0.12261520383930957
2101	33	0.1754129149017274
2101	34	0.0611551940798023
2101	35	0.08432798174295897
2101	36	0.16106480674058332
2101	37	0.13873481823390676
2101	38	0.06921003589060604
2101	39	-0.09349785365274954
2101	40	0.0731417524646929
2101	41	-0.034865220190305024
2101	42	-2.235714287869804
2101	43	-0.9885901902388596
2101	44	-0.42866267931771773
2101	45	-0.28578215833756104
2101	46	-0.09940743790124884
2101	47	0.011466680572432608
2101	48	0.14385997361533498
2101	49	0.03296105351041234
2101	50	0.2860099627115744
2101	51	0.265503692839431
2104	1	0.1512289886155962
2104	2	0.06421387251514687
2104	3	0.1715340883937202
2104	4	0.19867363211218247
2104	5	0.06717605358933554
2104	6	0.048291629730130126
2104	7	0.12474664993720097
2104	8	-0.16566779064626758
2104	9	-0.025380893806357108
2104	10	0.07820911945662345
2104	11	-0.023970795552152424
2104	12	0.06049094005093991
2104	13	0.7108209863018757
2104	14	0.417738663790054
2104	15	0.1514737909976489
2104	16	0.371982020214233
2104	17	0.22724215602475084
2104	18	0.24339437380691464
2104	19	0.1417360936182348
2104	20	-0.05644642283333212
2104	21	0.057928152303333036
2104	22	0.147393819633848
2104	23	0.11737320887455382
2104	24	0.21056662625067446
2104	25	0.022542735507407485
2104	26	0.2579210148067048
2104	27	0.05795932765215639
2104	28	0.16520988022209337
2104	29	0.15567931538979707
2104	30	0.2612781951135972
2104	31	0.29860422128634667
2104	32	0.261928590643592
2104	33	0.27411482415753596
2104	34	0.24100600117202475
2104	35	0.2159752701074069
2104	36	0.15205729371611199
2104	37	0.28440081615276824
2104	38	-0.10019440975806049
2104	39	0.09082428774707552
2104	40	0.09387432095185488
2104	41	0.2745319993014306
2104	42	0.6933886625187995
2104	43	0.2620725755070552
2104	44	0.22781799829031968
2104	45	0.35417885074915034
2104	46	0.3394061307117795
2104	47	0.2013008054291774
2104	48	0.2796346193495509
2104	49	0.3137333066617174
2104	50	0.15511821676400928
2104	51	0.6991176030865986
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	5.0
11	10.0
12	8.0
13	15.0
14	10.0
15	23.0
16	38.0
17	82.0
18	178.0
19	393.0
20	812.0
21	1789.0
22	3184.0
23	5334.0
24	8824.0
25	13435.0
26	19867.0
27	28161.0
28	38543.0
29	50931.0
30	66037.0
31	82988.0
32	102068.0
33	123906.0
34	147288.0
35	174337.0
36	204062.0
37	237003.0
38	258678.0
39	5746.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	21.30727133158591	25.12937009968977	20.34436315539561	33.21899541332872
2	18.34029225567574	28.635070554004777	26.450428559900214	26.57420863041927
3	18.2132715364932	29.894666210010556	25.251769807194112	26.640292446302134
4	20.07327678507319	28.765459006112117	26.69322304113908	24.46804116767561
5	18.566565782943893	29.37228635388265	27.970737577990022	24.090410285183435
6	19.44713281407377	27.558651823053527	30.369313145036607	22.6249022178361
7	17.152054412397323	31.711462809744056	30.085292601980356	21.05119017587826
8	21.437653414531706	28.697659552701527	28.846284596307314	21.018402436459457
9	17.443966960633745	31.85182737636449	29.734285872233297	20.969919790768472
10	19.047198459230415	27.92428828048005	31.781422695596973	21.247090564692563
11	18.374986259014246	29.500323747155694	27.864749304054815	24.259940689775245
12	24.081514386891513	28.793354001470366	27.48356006224587	19.64157154939225
13	21.530170756767713	29.251937558419044	28.73216292964806	20.48572875516518
14	17.881136819551152	33.3462112051464	28.55278349480448	20.219868480497965
15	18.643261134646412	32.464691226547394	28.302109789364195	20.589937849442006
16	22.918439227353108	28.37511969748863	28.35942479121644	20.347016283941823
17	18.771084475154236	31.927097994635673	28.457949240307574	20.843868289902517
18	23.342222933898608	28.41965268921537	28.2464992829225	19.99162509396352
19	18.467122348466315	28.685586547876774	28.79386233851562	24.053428765141298
20	18.576033560411727	32.88451408379555	28.31138694044005	20.22806541535267
21	18.712986544258563	28.997898016968364	28.852640060187372	23.436475378585698
22	19.115113751390798	32.23679403001387	28.519023802549214	20.129068416046117
23	18.38659765973738	28.854745497234326	28.34011647302153	24.418540370006767
24	22.864971411795953	29.04527217571547	28.28713511754905	19.802621294939527
25	18.365645565807725	28.135947117697178	32.21166646227282	21.28674085422228
26	18.06681323095443	28.62160291501496	29.133560997374424	24.178022856656188
27	18.8100660615473	28.102528843147674	32.268231741569465	20.819173353735565
28	22.846860838791553	28.06844850189468	28.530059016751807	20.55463164256196
29	18.235701908614978	32.71574618477162	29.164567128766222	19.88398477784718
30	19.15100653438577	31.95162736776942	28.90485321625124	19.992512881593573
31	18.390556999041134	27.6258358289854	29.739912069170387	24.243695102803073
32	18.70466660354807	28.209946008182968	32.68026766521134	20.405119723057627
33	18.625355785893625	29.27246511089971	28.40497982688423	23.697199276322436
34	18.37568522245147	28.675737517625	28.315898431716676	24.63267882820686
35	18.356548474715005	32.25519242575031	28.45955176283823	20.928707336696448
36	22.79484978322602	28.58213995916783	28.843107489774482	19.77990276783167
37	18.780501443735005	27.649685835129528	32.56753528000325	21.002277441132215
38	17.98127862266785	28.817144719894213	28.569195448459656	24.63238120897828
39	18.328791130026897	32.527407309505456	29.019373360215887	20.124428200251753
40	18.59585870517659	28.03663076748092	28.48962261693182	24.877887910410678
41	18.09946758048723	28.929143534683522	28.926411223065283	24.044977661763966
42	19.0759195022872	32.21916443369029	28.867253499423988	19.837662564598517
43	22.161398282710376	28.377852009106856	28.595356722344402	20.865392985838366
44	18.107374268377846	32.024700567886846	29.248555500978885	20.61936966275642
45	18.29435129521102	28.091975963282817	32.43597018349061	21.177702558015554
46	18.961204842245735	28.260223048327138	28.334699583770217	24.44387252565691
47	18.587711851482833	32.30970221332059	29.19521853617869	19.907367399017883
48	18.60523340897263	29.225193922337017	32.32337610099022	19.846196567700137
49	18.23290089618331	28.08284643375984	29.775121666639127	23.909131003417723
50	18.516296867712416	28.050793715345247	32.79561146772843	20.63729794921391
51	18.485896026485857	28.307586724027274	28.6628708687336	24.54364638075327
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	167.0
1	275.5
2	384.0
3	502.0
4	620.0
5	708.5
6	797.0
7	830.0
8	863.0
9	897.0
10	931.0
11	1080.0
12	1229.0
13	1495.5
14	1762.0
15	2182.0
16	2602.0
17	3192.0
18	3782.0
19	4894.0
20	6006.0
21	7708.5
22	9411.0
23	12264.5
24	15118.0
25	19536.5
26	30174.0
27	36393.0
28	44547.0
29	52701.0
30	62296.0
31	71891.0
32	83016.5
33	94142.0
34	104147.5
35	114153.0
36	122942.5
37	131732.0
38	135895.0
39	140058.0
40	139518.5
41	138979.0
42	134553.0
43	130127.0
44	123245.5
45	116364.0
46	108963.0
47	101562.0
48	96221.0
49	90880.0
50	91536.0
51	92192.0
52	83626.0
53	75060.0
54	62985.0
55	50910.0
56	39189.5
57	27469.0
58	20447.0
59	13425.0
60	11295.0
61	9165.0
62	8286.5
63	7408.0
64	5872.0
65	4336.0
66	3509.0
67	2682.0
68	2110.0
69	1538.0
70	1207.0
71	876.0
72	770.0
73	664.0
74	573.0
75	451.5
76	421.0
77	323.5
78	226.0
79	191.5
80	157.0
81	115.5
82	74.0
83	57.0
84	40.0
85	28.5
86	17.0
87	11.5
88	6.0
89	3.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17061062081297074
2	0.0
3	0.0
4	0.0
5	0.0
6	0.00686255011091279
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.001906263919697997
18	6.35421306565999E-4
19	0.0
20	0.0
21	4.447949145961993E-4
22	0.001270842613131998
23	2.5416852262639955E-4
24	0.0020968903116677963
25	0.0
26	0.02465434669476076
27	0.0047656597992449925
28	0.008768814030610786
29	0.0
30	0.024781430956073963
31	0.0013979268744451977
32	1.2708426131319978E-4
33	0.008260476985357985
34	0.0
35	0.013788642352482177
36	0.0
37	0.004003154231365793
38	0.0031771065328299953
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.001906263919697997
45	0.0
46	0.006926092241569389
47	9.531319598489985E-4
48	0.003939612100709194
49	0.01251779973935018
50	0.0013343847437885979
51	0.005655249628437391
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1573759.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.91527620164459
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.95902332751496	59.76064823142785
2	13.366016720348908	19.491736017600807
3	2.8105065646966327	6.147865872941708
4	0.6535242460635605	1.9060760362478422
5	0.26889426865341437	0.9803249933951467
6	0.16778291188649438	0.7340362417271961
7	0.10997221474924321	0.561305808906335
8	0.08789879537034362	0.5127331953776354
9	0.06304927019219514	0.41375294553384223
>10	0.48242490770031043	6.532126173412337
>50	0.02028654510564835	0.9917585818608019
>100	0.01008439224287375	1.2480463444777956
>500	3.612926491260868E-4	0.17330067591171133
>1k	8.727439876965634E-5	0.11740910065042522
>5k	8.726842745441067E-5	0.4288797805285517
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC	6740	0.42827396062548334	TruSeq Adapter, Index 7 (100% over 50bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCA	1845	0.11723523106142682	TruSeq Adapter, Index 7 (100% over 40bp)
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.6107034177405817	0.0	0.0
2	0.6164857516303323	0.0	0.0
3	0.626525408274075	0.0	0.0
4	0.7066520350320474	0.0	0.0
5	0.7259053006209972	0.0	0.0
6	0.9429016768132858	0.0	0.0
7	4.747995086922457	0.0	0.0
8	4.801179850282032	0.0	0.0
9	4.856969840998526	0.0	0.0
10	5.086547559060822	0.0	0.0
11	5.6585538192315346	0.0	0.0
12	5.902110806038282	0.0	0.0
13	6.099218495335054	0.0	0.0
14	6.1707033923237296	0.0	0.0
15	6.2486695866393775	0.0	0.0
16	6.3181846775776975	0.0	0.0
17	6.384268493460562	0.0	0.0
18	6.452258573263124	0.0	0.0
19	6.551511381348733	0.0	0.0
20	6.592686682014209	0.0	0.0
21	6.646824577333632	0.0	0.0
22	6.691367610923909	0.0	0.0
23	6.75058887669586	0.0	0.0
24	6.797610053381744	0.0	0.0
25	6.917514053930748	0.0	0.0
26	7.019753342157217	0.0	0.0
27	7.117862391891007	0.0	0.0
28	7.163612725963759	0.0	0.0
29	7.209045349383229	0.0	0.0
30	7.256956115898305	0.0	0.0
31	7.3135086121826784	0.0	0.0
32	7.371331951080184	0.0	0.0
33	7.421784402821525	0.0	0.0
34	7.471283722603016	0.0	0.0
35	7.519003862726122	0.0	0.0
36	7.570790699211251	0.0	0.0
37	7.618892092118298	0.0	0.0
38	7.669789338774234	0.0	0.0
39	7.719669911339665	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCATCTC	7785	0.0	33.377663	45
GATCATC	7960	0.0	32.813435	43
ATCATCT	8170	0.0	31.61203	44
AGATCAT	8745	0.0	31.307579	42
CGTATGC	1425	0.0	29.838669	45
CAGATCA	10100	0.0	28.688854	41
CTCGTAT	1370	0.0	28.573355	43
CCCCGAG	135	0.0	28.329172	2
TCGTATG	1485	0.0	28.178576	44
CGTAAGA	645	0.0	27.205307	11
TCGTAAG	680	0.0	26.4667	10
AGTCACC	11395	0.0	26.400053	35
TCACCAG	11300	0.0	26.319143	37
GTCACCA	11415	0.0	26.034285	36
TAAGAGC	685	0.0	25.945095	13
CACCAGA	11490	0.0	25.786028	38
GAGATCG	3185	0.0	25.639877	6
ACCAGAT	11480	0.0	25.632124	39
GTAAGAG	705	0.0	25.528166	12
CAGTCAC	11885	0.0	25.254826	34
>>END_MODULE