FastQCFastQC Report
Sun 19 Apr 2015
HCNNTADXX l01n01 rnh201-frbrad27-frb.341000000088e3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCNNTADXX l01n01 rnh201-frbrad27-frb.341000000088e3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1573759
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC67400.42827396062548334TruSeq Adapter, Index 7 (100% over 50bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCA18450.11723523106142682TruSeq Adapter, Index 7 (100% over 40bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATCTC77850.033.37766345
GATCATC79600.032.81343543
ATCATCT81700.031.6120344
AGATCAT87450.031.30757942
CGTATGC14250.029.83866945
CAGATCA101000.028.68885441
CTCGTAT13700.028.57335543
CCCCGAG1350.028.3291722
TCGTATG14850.028.17857644
CGTAAGA6450.027.20530711
TCGTAAG6800.026.466710
AGTCACC113950.026.40005335
TCACCAG113000.026.31914337
GTCACCA114150.026.03428536
TAAGAGC6850.025.94509513
CACCAGA114900.025.78602838
GAGATCG31850.025.6398776
ACCAGAT114800.025.63212439
GTAAGAG7050.025.52816612
CAGTCAC118850.025.25482634
Produced by FastQC (version 0.11.2)