##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename HCNNTADXX l01n01 rnh201-frb rad27-frb dna2-frb.3410000000891b.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3272273 Sequences flagged as poor quality 0 Sequence length 51 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.429357208276937 33.0 33.0 33.0 27.0 33.0 2 31.616128299808725 33.0 33.0 33.0 27.0 33.0 3 31.597978530519917 33.0 33.0 33.0 27.0 33.0 4 35.23203473548814 37.0 37.0 37.0 33.0 37.0 5 35.29714666227421 37.0 37.0 37.0 33.0 37.0 6 34.94478975317769 37.0 33.0 37.0 33.0 37.0 7 34.905820510697 37.0 33.0 37.0 33.0 37.0 8 34.89000184275579 37.0 33.0 37.0 27.0 37.0 9 34.969646481207405 37.0 33.0 37.0 33.0 37.0 10 34.862108998851866 37.0 33.0 37.0 27.0 37.0 11 34.95805637243592 37.0 33.0 37.0 33.0 37.0 12 34.7592719189383 37.0 33.0 37.0 27.0 37.0 13 34.22319592527885 37.0 33.0 37.0 27.0 37.0 14 35.60329654646785 37.0 33.0 40.0 27.0 40.0 15 35.711727902898076 37.0 33.0 40.0 27.0 40.0 16 35.49858034461061 37.0 33.0 40.0 27.0 40.0 17 35.738297507573485 37.0 33.0 40.0 27.0 40.0 18 35.66448887363615 37.0 33.0 40.0 27.0 40.0 19 35.79916162251744 37.0 33.0 40.0 27.0 40.0 20 35.72976704572021 37.0 33.0 40.0 27.0 40.0 21 35.725142125977875 37.0 33.0 40.0 27.0 40.0 22 35.72364439030606 37.0 33.0 40.0 27.0 40.0 23 35.67076035526376 37.0 33.0 40.0 27.0 40.0 24 35.574187422626416 37.0 33.0 40.0 27.0 40.0 25 35.635135271415315 37.0 33.0 40.0 27.0 40.0 26 35.45612270125384 37.0 33.0 40.0 27.0 40.0 27 35.34722591910883 37.0 33.0 40.0 27.0 40.0 28 35.14120062720928 37.0 33.0 40.0 27.0 40.0 29 35.214725055030556 37.0 33.0 40.0 27.0 40.0 30 35.10772634190362 37.0 33.0 40.0 27.0 40.0 31 35.107573237318526 37.0 33.0 40.0 27.0 40.0 32 35.07274759777072 37.0 33.0 40.0 27.0 40.0 33 34.98185878745447 37.0 33.0 40.0 27.0 40.0 34 34.9926127801684 37.0 33.0 40.0 27.0 40.0 35 34.98254271572085 37.0 33.0 40.0 27.0 40.0 36 34.92011088316898 37.0 33.0 40.0 27.0 40.0 37 34.94817088916481 37.0 33.0 40.0 27.0 40.0 38 34.89072855473856 37.0 33.0 40.0 27.0 40.0 39 34.84393172574538 37.0 33.0 40.0 27.0 40.0 40 34.869307664733356 37.0 33.0 40.0 27.0 40.0 41 34.69654701792913 37.0 33.0 40.0 27.0 40.0 42 34.36068475949287 37.0 33.0 40.0 27.0 40.0 43 34.506973898571424 37.0 33.0 40.0 27.0 40.0 44 34.45025674813807 37.0 33.0 40.0 27.0 40.0 45 34.474651717628696 37.0 33.0 40.0 27.0 40.0 46 34.28130140730923 37.0 33.0 40.0 27.0 40.0 47 34.38208914720746 37.0 33.0 40.0 27.0 40.0 48 34.205366422667055 37.0 33.0 40.0 22.0 40.0 49 34.21832255438345 37.0 33.0 40.0 22.0 40.0 50 34.16861245990172 37.0 33.0 40.0 22.0 40.0 51 33.051481340340494 37.0 33.0 37.0 22.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.028209402592825228 2201 2 0.04171844513064826 2201 3 -0.4385928195765274 2201 4 -0.2220728678482473 2201 5 -0.06034906144820695 2201 6 -0.21778158927401847 2201 7 -0.00669481890383139 2201 8 0.041635659917645285 2201 9 -0.15905022519414302 2201 10 0.09997043670805539 2201 11 0.01323966106611607 2201 12 0.03477446827728414 2201 13 0.7752640051349431 2201 14 -0.030799306148189487 2201 15 -0.03793399564128919 2201 16 0.31738668730758235 2201 17 0.09312173719399652 2201 18 0.14764251487031999 2201 19 0.039591741712598605 2201 20 0.07463793846174127 2201 21 -0.025479588295212352 2201 22 -0.06599494439564779 2201 23 -0.31893032176031255 2201 24 -0.04451395765990185 2201 25 -0.13125305691507094 2201 26 -0.17509146991846336 2201 27 -0.2554975101344965 2201 28 -0.0482359353589672 2201 29 -0.09627267124628247 2201 30 -0.22747134337881647 2201 31 -0.3201789011228513 2201 32 -0.2822453796651274 2201 33 -0.49115019704982643 2201 34 -0.2865985241106799 2201 35 -0.41322039511513253 2201 36 -0.17031713592355402 2201 37 -0.22283458193607686 2201 38 -0.18310367824178542 2201 39 -0.16302075600160038 2201 40 -0.1366129928898161 2201 41 -0.020090475678479436 2201 42 0.24281459620882373 2201 43 0.06366138527381793 2201 44 -0.14152068776347448 2201 45 -0.18913477134160672 2201 46 -0.30068758256511785 2201 47 -0.16989621033069824 2201 48 -0.18984145133872232 2201 49 -0.1607408720246113 2201 50 -0.2147911527612223 2201 51 -0.3975202406923515 1101 1 0.12356175019356286 1101 2 0.06181058646979665 1101 3 0.1223324788839939 1101 4 -0.5843213881252254 1101 5 -0.16675600630910736 1101 6 -0.23011214663756618 1101 7 -0.17140378262597267 1101 8 -0.11905697424572992 1101 9 -0.09068359734024511 1101 10 -0.16799036722289884 1101 11 -0.11048364692725698 1101 12 -0.19033751936875376 1101 13 0.2384258344998642 1101 14 -0.15780079465639574 1101 15 -0.1208490734484684 1101 16 0.09620969354100595 1101 17 -0.07355688721545306 1101 18 -0.32101807769542745 1101 19 -0.21500629843820462 1101 20 -0.11293146097834494 1101 21 -0.312229250833866 1101 22 -0.2198083297375888 1101 23 -0.07558700162297782 1101 24 -0.13827468256408082 1101 25 -0.07321201257924059 1101 26 -0.057659629697440096 1101 27 -0.13855238791064295 1101 28 -0.0769262594996718 1101 29 -0.09064537281794571 1101 30 -0.39417421618815496 1101 31 -0.26377299312486713 1101 32 -0.055078846390955505 1101 33 -0.05976977429782693 1101 34 0.006442807356421554 1101 35 -0.2164937209064206 1101 36 -0.6801430635299752 1101 37 -0.2669375800979239 1101 38 -0.2903878214455986 1101 39 -0.09902044703052582 1101 40 -0.19414114430446006 1101 41 -0.06952969235810968 1101 42 0.052917021961349064 1101 43 -0.012734904882272247 1101 44 -0.22384486982759455 1101 45 -0.1740989160589521 1101 46 -0.1439508930113078 1101 47 -0.11938244710148638 1101 48 -0.440146933556683 1101 49 -0.28249276053436034 1101 50 -0.3326901542669276 1101 51 -0.27000430572795864 1102 1 -0.007762759040826239 1102 2 -0.0938999592765164 1102 3 0.053455434309146455 1102 4 0.05549661263962946 1102 5 -0.1141505937645988 1102 6 -0.1324581109314238 1102 7 -0.3646514283550104 1102 8 -0.15195132964127112 1102 9 -0.02156001305461075 1102 10 -0.21894580340175196 1102 11 -0.12751953661221904 1102 12 -0.5898154271860321 1102 13 0.5385090351567285 1102 14 0.07739535838688738 1102 15 0.027008301458593564 1102 16 -0.7613978703682918 1102 17 -0.47612958513457926 1102 18 -0.6201051316149346 1102 19 -0.3452802834403954 1102 20 -0.22450520141172348 1102 21 -0.2331010905384403 1102 22 -0.32408451837307695 1102 23 -0.10793002560904341 1102 24 -0.530476024396819 1102 25 -0.24641771441922344 1102 26 -0.22760836396884798 1102 27 -0.11751751286184486 1102 28 -0.31778842590412637 1102 29 -0.19312693090978428 1102 30 -0.24991455298827958 1102 31 -0.2381244435979042 1102 32 -0.20485330770826948 1102 33 -0.3531559941591169 1102 34 -0.37886553628275266 1102 35 -0.2585224344988859 1102 36 -0.17532962882680891 1102 37 -0.16516279777292198 1102 38 -0.2657686833528885 1102 39 -0.23025168929939355 1102 40 -0.172167808634768 1102 41 -0.12717388868934876 1102 42 0.1376628209247741 1102 43 -0.1585957577861805 1102 44 -0.08896643340796828 1102 45 -0.3656385996924598 1102 46 -0.3212095264304935 1102 47 -0.301376478802176 1102 48 -0.20428272919580337 1102 49 -0.34089883984979963 1102 50 -0.25724813494083065 1102 51 -0.5644590371598781 1103 1 -0.23013127430446545 1103 2 -0.10875840145262927 1103 3 -0.03377459901741986 1103 4 0.12611206023393606 1103 5 0.026943064239546288 1103 6 0.1457251733513374 1103 7 0.0985524939871425 1103 8 0.05221299561107173 1103 9 0.032467316804577706 1103 10 0.20616217970582085 1103 11 0.06657782658548683 1103 12 0.1758857922238306 1103 13 0.7825982890986509 1103 14 0.43169788730395453 1103 15 0.25748570920349323 1103 16 0.5280253934148362 1103 17 0.1741044624126502 1103 18 0.16815584148901763 1103 19 0.027220482901057608 1103 20 0.09641107470810084 1103 21 0.058134045245019195 1103 22 0.16053869724577652 1103 23 0.017966082362171676 1103 24 0.11401843780274845 1103 25 0.09235790004434108 1103 26 0.10436186221392063 1103 27 0.16638501561246244 1103 28 0.2032800469530187 1103 29 0.11251874926959005 1103 30 0.12111729704776053 1103 31 -0.01236576049051763 1103 32 -0.18615634454599217 1103 33 -0.05706132817974918 1103 34 -0.013398550592569336 1103 35 0.09075081713481126 1103 36 0.14412461019004041 1103 37 -0.10142988481873516 1103 38 0.14682495759161185 1103 39 -0.16588890628982256 1103 40 -0.044534966168292556 1103 41 0.02206261395338771 1103 42 0.2462455709421647 1103 43 0.16081981270441048 1103 44 0.07973297081907305 1103 45 0.09780871293085625 1103 46 -0.08764506535692362 1103 47 -0.07259259710337318 1103 48 0.030655364050986122 1103 49 -0.01351448432387059 1103 50 -0.044605172405340454 1103 51 0.038163817407699696 2103 1 -0.03328982159536764 2103 2 -0.05745887362039426 2103 3 0.08197828237937799 2103 4 0.2105663091751211 2103 5 0.0059907585288314635 2103 6 0.11083465459832809 2103 7 0.10655548431679307 2103 8 0.07388601329107303 2103 9 0.04214455857192689 2103 10 0.13648498209034443 2103 11 0.017885962227829566 2103 12 0.19203396936562456 2103 13 0.8587969974956664 2103 14 0.5075442804158143 2103 15 0.33930171574036905 2103 16 -0.2782745380413161 2103 17 0.0962731742297791 2103 18 0.18651383540684918 2103 19 0.19762650616968358 2103 20 0.2671413484265983 2103 21 0.2061855263380039 2103 22 0.15602726086293472 2103 23 0.19988437895816702 2103 24 0.18878788801731616 2103 25 0.16148637707104996 2103 26 0.16013105656151794 2103 27 0.0895197826178844 2103 28 0.444343337944872 2103 29 0.38166810987534205 2103 30 0.31584665527913813 2103 31 0.30880718489289194 2103 32 0.0891403094696841 2103 33 0.18763110331562416 2103 34 0.18327898304512047 2103 35 0.22840375293951354 2103 36 0.3066524876183152 2103 37 0.1757195947205048 2103 38 0.2353555329796606 2103 39 0.3317071715133011 2103 40 0.15311277480830654 2103 41 0.00417759379195104 2103 42 0.5703968444751979 2103 43 0.39988704647485207 2103 44 0.2837666285560658 2103 45 0.3187249772291878 2103 46 0.1604686541368423 2103 47 0.16531723710261303 2103 48 0.30939708102205543 2103 49 0.27327168069923147 2103 50 0.3570737319698125 2103 51 0.12084712952159293 2102 1 -0.16107813445210084 2102 2 -0.05071099188153383 2102 3 -0.14273960852147027 2102 4 0.055199727883696426 2102 5 0.014419863970587699 2102 6 -0.0031664244503346595 2102 7 6.602050349755473E-4 2102 8 0.01610369696996372 2102 9 -0.0046061662443435125 2102 10 -0.17723215103916345 2102 11 -0.06632045942795628 2102 12 -0.018981973536511987 2102 13 0.53338436418165 2102 14 0.18564967975922286 2102 15 0.0725411569922656 2102 16 -0.25958613009236586 2102 17 -0.11969475084848824 2102 18 -0.04616689075051994 2102 19 -0.017259024171863757 2102 20 0.06374977719045916 2102 21 0.11323954575206585 2102 22 0.13069103749323574 2102 23 0.07000023566533997 2102 24 -0.015717688833156274 2102 25 0.09586249637824551 2102 26 -0.035095434699094596 2102 27 0.0023101688339366433 2102 28 -0.6686179572881201 2102 29 -0.3629263111322132 2102 30 -0.0909560390783355 2102 31 -0.1539328243691216 2102 32 0.06546157736618596 2102 33 0.12935098385543142 2102 34 -0.015292204705346535 2102 35 0.02979088411942854 2102 36 7.165302735856471E-4 2102 37 0.07945132246991449 2102 38 0.1732712076027383 2102 39 0.10874456096215113 2102 40 0.02932037776036367 2102 41 -0.12335138356210251 2102 42 0.29683478662703777 2102 43 0.17293768313481195 2102 44 0.17149038841738928 2102 45 0.1302255079824448 2102 46 0.2039338632319314 2102 47 0.10870541123505006 2102 48 -0.014200944048894826 2102 49 0.04949392250163953 2102 50 -0.0234626328926808 2102 51 -0.011070459260231758 2101 1 0.15789415342930724 2101 2 0.12617213510935343 2101 3 0.21202280998357637 2101 4 0.14378531648162607 2101 5 0.19656850788003055 2101 6 0.2011059049433257 2101 7 0.1765063806662539 2101 8 0.23606198675188494 2101 9 0.22580707894573493 2101 10 0.03328447011829638 2101 11 0.24661136541088524 2101 12 0.307861363972755 2101 13 -4.499131335643028 2101 14 -1.4522945005268397 2101 15 -0.7164008330003782 2101 16 -0.00989627803200932 2101 17 0.04104292449121516 2101 18 0.20308981633191792 2101 19 0.11945893334449664 2101 20 -0.13239644643135762 2101 21 0.0742363133585684 2101 22 -0.029675985729248566 2101 23 0.0404535003915143 2101 24 0.20299554218604499 2101 25 0.12715566831608527 2101 26 -0.031449474201153294 2101 27 0.1663061809517643 2101 28 0.26686200957680484 2101 29 0.03629799246786547 2101 30 0.1879451990305796 2101 31 0.38569431215827876 2101 32 0.3011811489098335 2101 33 0.3330095446195358 2101 34 0.2804642829741155 2101 35 0.2593114252726494 2101 36 0.3578063257348134 2101 37 0.22909894815852994 2101 38 0.232355514388928 2101 39 0.044718677919796335 2101 40 0.1738823116956425 2101 41 0.0744137373523941 2101 42 -2.091314491672321 2101 43 -0.856983417971314 2101 44 -0.3751621516688175 2101 45 -0.17820125278026921 2101 46 0.08950414342924518 2101 47 0.11684528713097819 2101 48 0.1309836736920147 2101 49 0.11912573379031244 2101 50 0.37732383780071643 2101 51 0.3622242524908401 2104 1 0.12259668317708616 2104 2 0.08112705952128962 2104 3 0.1453180215593335 2104 4 0.2152342295594991 2104 5 0.0973334669029029 2104 6 0.12585253840036614 2104 7 0.16047546587967787 2104 8 -0.14889204865463768 2104 9 -0.024518952488897128 2104 10 0.08826625304125457 2104 11 -0.0399911723228783 2104 12 0.08857932625180354 2104 13 0.7721528100755108 2104 14 0.43860739546555294 2104 15 0.17884701869543562 2104 16 0.36753304227061534 2104 17 0.2648389248709293 2104 18 0.2818880919627702 2104 19 0.19364794192264867 2104 20 -0.03210702996546644 2104 21 0.11901449897385419 2104 22 0.192306782633608 2104 23 0.1741431516150982 2104 24 0.22318048544791225 2104 25 -0.02597965789616552 2104 26 0.26241145370955365 2104 27 0.08704626289094364 2104 28 0.19708318357625387 2104 29 0.21248643449341387 2104 30 0.337607000276094 2104 31 0.29387342565413377 2104 32 0.2725508425645913 2104 33 0.31114566189592807 2104 34 0.22396874231567665 2104 35 0.2799796710540221 2104 36 0.2164898744636119 2104 37 0.27209497927673 2104 38 -0.04854702952266621 2104 39 0.17301138822608664 2104 40 0.19114144773305952 2104 41 0.23949149519032886 2104 42 0.544442850532981 2104 43 0.23100815305193123 2104 44 0.29450415487531956 2104 45 0.360314341730799 2104 46 0.3995864065658097 2104 47 0.2723797978691067 2104 48 0.37743593937504727 2104 49 0.3557556197414655 2104 50 0.13839967749646576 2104 51 0.721818843420273 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 2.0 9 3.0 10 9.0 11 16.0 12 15.0 13 13.0 14 19.0 15 24.0 16 48.0 17 108.0 18 305.0 19 732.0 20 1508.0 21 3078.0 22 5843.0 23 9879.0 24 16461.0 25 25813.0 26 38479.0 27 55046.0 28 75838.0 29 102735.0 30 133143.0 31 169097.0 32 209472.0 33 256126.0 34 308237.0 35 366510.0 36 430541.0 37 497839.0 38 552139.0 39 13194.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 21.633448597604428 25.22861019207174 23.29139289361483 29.846548316708994 2 18.42187372508345 29.787765262861626 23.300775943816422 28.489585068238497 3 17.639451231605676 30.862339419724456 24.92383734486701 26.57437200380286 4 18.58717166935644 29.533141030714738 25.497108584766615 26.382578715162214 5 18.017353686565883 30.07166578094187 27.717186188316195 24.19379434417605 6 18.45176898599163 29.01348514873272 29.753634119834576 22.781111745441077 7 17.24877478132173 30.49485785568625 29.76851259048374 22.487854772508285 8 18.26082359265257 29.762492310390975 29.194446795851082 22.78223730110538 9 18.283254483962676 29.574732915010454 29.569812787624993 22.572199813401877 10 18.606332662342048 29.294621811810934 29.057049946627313 23.041995579219705 11 18.327382831444687 29.884700940294408 29.045101065834057 22.74281516242685 12 19.60450732564184 29.91443562318914 28.678994692679982 21.80206235848904 13 18.302782194517388 29.87085735206079 29.775816382068363 22.05054407135346 14 18.256636900405315 30.065614941051678 29.428259805951395 22.24948835259161 15 18.897048015248117 30.270029426028938 29.201292190474327 21.631630368248615 16 19.237209120388183 29.411421357570106 29.2189557533861 22.13241376865561 17 18.489884481388668 29.858963388545934 29.50941262759 22.1417395024754 18 19.412596832715668 29.885091895007342 29.283337820538485 21.418973451738506 19 18.655434501258913 29.533696649022435 29.572874357212076 22.237994492506576 20 18.621734800244354 30.139233493049023 29.498669579219094 21.74036212748753 21 18.956749689969055 29.971084151780392 29.424580121225308 21.647586037025242 22 18.6664099602105 29.95920200964483 29.55018305615148 21.82420497399319 23 18.49375937041793 29.66794264280471 29.53155397513527 22.306744011642085 24 19.425198274934726 29.964268949802946 29.34802140684745 21.262511368414877 25 18.59468937952304 29.531949198615155 29.862422847971427 22.01093857389038 26 18.483756234082378 29.65167389467687 29.692908398878572 22.171661472362185 27 18.92472638500591 29.679937997308166 29.86920390352909 21.526131714156833 28 19.148105767479276 29.365463585429037 29.59425807137801 21.892172575713676 29 18.508266272404533 29.88378414637165 29.860191982759382 21.747757598464432 30 19.05755795044103 29.964516894213393 29.68338480951368 21.294540345831898 31 18.63850271726757 29.31217054104842 29.76773268064718 22.28159406103683 32 18.654345748681266 29.324807541682873 30.081255594363178 21.939591115272684 33 18.9765307890705 29.758810313848855 29.47393771800578 21.79072117907487 34 18.637900933082292 29.499830851521253 29.54136161622212 22.320906599174336 35 18.53736049185599 29.84257845403765 29.748533857888436 21.871527196217922 36 19.372466783792184 29.579897520775315 29.49870013901652 21.548935556415984 37 18.512046432766983 29.26154248615293 30.027486330180686 22.1989247508994 38 18.477844312109244 29.390965361739426 29.692172942537688 22.439017383613646 39 18.82599648623449 30.045139876776783 29.55706935209868 21.571794284890046 40 18.69119721979187 29.242120079834415 29.62341467230882 22.443268028064896 41 18.98127081695201 29.506492887359947 29.650887930194088 21.861348365493953 42 19.658445368097343 29.647831950451565 29.277233287075987 21.416489394375102 43 18.65593121356317 29.310176748700368 29.580967113685197 22.452924924051263 44 18.61746944044359 29.38225062702137 30.03648596654806 21.963793965986973 45 18.981179137559735 29.939769695254643 29.387829193957838 21.691221973227783 46 18.826605005224536 29.215225510771347 29.49483521242422 22.4633342715799 47 18.629182421688895 29.67016142168186 29.52197533369374 22.178680822935505 48 18.905334123845556 29.645675307291253 29.793162884228668 21.65582768463452 49 18.620915406238147 29.360509096266103 29.52778515555634 22.490790341939405 50 18.642755268780135 29.367434671037973 29.729173867376147 22.260636192805737 51 18.824787826740707 29.622993255075503 29.355946810753984 22.196272107429806 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 61.0 1 116.0 2 171.0 3 230.5 4 290.0 5 368.0 6 446.0 7 476.0 8 506.0 9 604.5 10 703.0 11 882.0 12 1061.0 13 1390.5 14 1720.0 15 2447.0 16 3174.0 17 4442.5 18 5711.0 19 7689.5 20 9668.0 21 12863.0 22 16058.0 23 21885.0 24 27712.0 25 37101.5 26 60713.0 27 74935.0 28 92255.5 29 109576.0 30 131535.5 31 153495.0 32 177585.0 33 201675.0 34 226515.5 35 251356.0 36 269996.5 37 288637.0 38 300276.5 39 311916.0 40 312390.5 41 312865.0 42 304484.0 43 296103.0 44 280907.0 45 265711.0 46 245649.0 47 225587.0 48 206952.0 49 188317.0 50 166766.5 51 145216.0 52 127529.5 53 109843.0 54 93327.0 55 76811.0 56 62707.5 57 48604.0 58 39346.5 59 30089.0 60 25420.5 61 20752.0 62 18545.5 63 16339.0 64 13059.5 65 9780.0 66 8222.5 67 6665.0 68 5184.0 69 3703.0 70 3090.0 71 2477.0 72 2290.5 73 2104.0 74 1984.0 75 1707.5 76 1551.0 77 1362.5 78 1174.0 79 925.0 80 676.0 81 524.5 82 373.0 83 298.5 84 224.0 85 140.5 86 57.0 87 38.0 88 19.0 89 12.0 90 5.0 91 2.5 92 0.0 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1704319902404231 2 0.0 3 0.0 4 0.0 5 0.0 6 0.006998193610374196 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0022308652120406826 18 1.8335878455128895E-4 19 1.222391897008593E-4 20 0.0 21 5.806361510790818E-4 22 0.0016196692635363857 23 2.1391858197650378E-4 24 0.0014974300738355266 25 0.0 26 0.022644809892084186 27 0.004797888195758728 28 0.010420890921998256 29 0.0 30 0.02618974639340911 31 0.001589109466111171 32 1.8335878455128895E-4 33 0.008678982468761011 34 0.0 35 0.012865674716015444 36 0.0 37 0.0030865395399466977 38 0.003392137514198846 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0017113486558120302 45 0.0 46 0.006478677054145543 47 0.0011001527073077337 48 0.004064453057553572 49 0.013324071677393664 50 7.945547330555855E-4 51 0.005592442928814313 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 3272273.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.5079513529305 #Duplication Level Percentage of deduplicated Percentage of total 1 87.97451847874605 67.30750180069388 2 9.176670200389404 14.041764745465594 3 1.4514473571438302 3.3314179137509914 4 0.3619534993251472 1.107692828735653 5 0.22802664350188895 0.8722925674107272 6 0.11687169384818433 0.5364968320482885 7 0.09096892326848888 0.48718921692378103 8 0.05517399082756 0.33769992049455955 9 0.04001056702657074 0.2755013864104836 >10 0.42468169785803267 6.7506050860423175 >50 0.06474864865652036 3.3296751440669556 >100 0.014886645093685374 1.5809242495669702 >500 0.0 0.0 >1k 4.1654314590500674E-5 0.04123830838982617 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.06845394623248122 0.0 0.0 2 0.06891234319385944 0.0 0.0 3 0.07010417529344282 0.0 0.0 4 0.07930267431843248 0.0 0.0 5 0.08131962094849665 0.0 0.0 6 0.101000130490335 0.0 0.0 7 0.47352406110370376 0.0 0.0 8 0.479819379373298 0.0 0.0 9 0.48791772569097996 0.0 0.0 10 0.5268203478132785 0.0 0.0 11 0.6317015725766157 0.0 0.0 12 0.6798943731161795 0.0 0.0 13 0.721516817209322 0.0 0.0 14 0.742266919661043 0.0 0.0 15 0.7627114241385117 0.0 0.0 16 0.7852951144357454 0.0 0.0 17 0.8087344790608852 0.0 0.0 18 0.8337323933547109 0.0 0.0 19 0.8673787303198724 0.0 0.0 20 0.8899929804145315 0.0 0.0 21 0.916396645389917 0.0 0.0 22 0.9399888090021828 0.0 0.0 23 0.9694178939226648 0.0 0.0 24 0.9969217116053581 0.0 0.0 25 1.0397054280006588 0.0 0.0 26 1.0788830882997842 0.0 0.0 27 1.1197415374572965 0.0 0.0 28 1.1487733450112505 0.0 0.0 29 1.1807388931180254 0.0 0.0 30 1.2155159425879198 0.0 0.0 31 1.2543268853179426 0.0 0.0 32 1.2957354108291086 0.0 0.0 33 1.3357993052535653 0.0 0.0 34 1.3765049554239515 0.0 0.0 35 1.4210611400699147 0.0 0.0 36 1.4689483426352263 0.0 0.0 37 1.517996817502696 0.0 0.0 38 1.5700707123152622 0.0 0.0 39 1.62737033248754 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACATCTC 2235 0.0 24.966223 45 GCTACAT 2365 0.0 24.922737 42 GGCTACA 2625 0.0 23.91115 41 CGGCTAC 2700 0.0 23.663565 40 CACGGCT 2800 0.0 22.416702 38 ACGGCTA 2845 0.0 22.219267 39 TCACGGC 2935 0.0 21.615896 37 GTCACGG 2975 0.0 21.400883 36 AGTCACG 3110 0.0 21.197239 35 CTACATC 3245 0.0 18.096636 43 CAGTCAC 3730 0.0 17.734158 34 TACATCT 3315 0.0 17.10366 44 CCAGTCA 3900 0.0 16.903439 33 TCCAGTC 3980 0.0 16.733265 32 ACTCCAG 4370 0.0 15.500419 30 GAACTCC 4525 0.0 15.466789 28 CTGAACT 4705 0.0 14.92564 26 GTCTGAA 4755 0.0 14.674023 24 TGAACTC 4770 0.0 14.531061 27 TCTGAAC 4835 0.0 14.52433 25 >>END_MODULE