FastQCFastQC Report
Sun 19 Apr 2015
HCNNTADXX l01n01 rnh201-frb rad27-frb dna2-frb.3410000000891b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCNNTADXX l01n01 rnh201-frb rad27-frb dna2-frb.3410000000891b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3272273
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATCTC22350.024.96622345
GCTACAT23650.024.92273742
GGCTACA26250.023.9111541
CGGCTAC27000.023.66356540
CACGGCT28000.022.41670238
ACGGCTA28450.022.21926739
TCACGGC29350.021.61589637
GTCACGG29750.021.40088336
AGTCACG31100.021.19723935
CTACATC32450.018.09663643
CAGTCAC37300.017.73415834
TACATCT33150.017.1036644
CCAGTCA39000.016.90343933
TCCAGTC39800.016.73326532
ACTCCAG43700.015.50041930
GAACTCC45250.015.46678928
CTGAACT47050.014.9256426
GTCTGAA47550.014.67402324
TGAACTC47700.014.53106127
TCTGAAC48350.014.5243325
Produced by FastQC (version 0.11.2)