##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename HCNNTADXX l01n01 rnh201-frb.34100000008893.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2836582 Sequences flagged as poor quality 0 Sequence length 51 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.52339435278092 33.0 33.0 33.0 27.0 33.0 2 31.70717856913708 33.0 33.0 33.0 27.0 33.0 3 31.720702239526304 33.0 33.0 33.0 27.0 33.0 4 35.39728024784758 37.0 37.0 37.0 33.0 37.0 5 35.46767764866308 37.0 37.0 37.0 33.0 37.0 6 35.11625258850264 37.0 37.0 37.0 33.0 37.0 7 35.071367582534194 37.0 33.0 37.0 33.0 37.0 8 35.027822922094266 37.0 33.0 37.0 33.0 37.0 9 35.10395645181419 37.0 33.0 37.0 33.0 37.0 10 34.98975139798532 37.0 33.0 37.0 33.0 37.0 11 35.0675880337674 37.0 33.0 37.0 33.0 37.0 12 34.88844461397555 37.0 33.0 37.0 27.0 37.0 13 34.31915911473738 37.0 33.0 37.0 27.0 37.0 14 35.76646894043606 37.0 33.0 40.0 27.0 40.0 15 35.87606492602717 37.0 33.0 40.0 33.0 40.0 16 35.68208992371805 37.0 33.0 40.0 27.0 40.0 17 35.91629397634195 37.0 33.0 40.0 33.0 40.0 18 35.84689601781299 37.0 33.0 40.0 27.0 40.0 19 35.985223765785726 37.0 33.0 40.0 33.0 40.0 20 35.91226835677587 37.0 33.0 40.0 27.0 40.0 21 35.90206840486191 37.0 33.0 40.0 27.0 40.0 22 35.89929957956442 37.0 33.0 40.0 27.0 40.0 23 35.84724291418334 37.0 33.0 40.0 27.0 40.0 24 35.75939035078133 37.0 33.0 40.0 27.0 40.0 25 35.83123773612044 37.0 33.0 40.0 27.0 40.0 26 35.65202416147321 37.0 33.0 40.0 27.0 40.0 27 35.55815273452345 37.0 33.0 40.0 27.0 40.0 28 35.37509192401277 37.0 33.0 40.0 27.0 40.0 29 35.44330465327637 37.0 33.0 40.0 27.0 40.0 30 35.33562082816573 37.0 33.0 40.0 27.0 40.0 31 35.33475887529428 37.0 33.0 40.0 27.0 40.0 32 35.29919741435291 37.0 33.0 40.0 27.0 40.0 33 35.2076040812499 37.0 33.0 40.0 27.0 40.0 34 35.230678683006516 37.0 33.0 40.0 27.0 40.0 35 35.23180856396889 37.0 33.0 40.0 27.0 40.0 36 35.1784961619301 37.0 33.0 40.0 27.0 40.0 37 35.22227102900604 37.0 33.0 40.0 27.0 40.0 38 35.15529464686725 37.0 33.0 40.0 27.0 40.0 39 35.116482090064736 37.0 33.0 40.0 27.0 40.0 40 35.138382038664844 37.0 33.0 40.0 27.0 40.0 41 34.98661276141497 37.0 33.0 40.0 27.0 40.0 42 34.653439597374586 37.0 33.0 40.0 27.0 40.0 43 34.80927679862595 37.0 33.0 40.0 27.0 40.0 44 34.766084675147766 37.0 33.0 40.0 27.0 40.0 45 34.787442774437686 37.0 33.0 40.0 27.0 40.0 46 34.60865506444023 37.0 33.0 40.0 27.0 40.0 47 34.7233631885135 37.0 33.0 40.0 27.0 40.0 48 34.58068055145242 37.0 33.0 40.0 27.0 40.0 49 34.58558222536842 37.0 33.0 40.0 27.0 40.0 50 34.55237112835095 37.0 33.0 40.0 27.0 40.0 51 33.46568369960749 37.0 33.0 37.0 22.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.08380579771482033 2201 2 0.07617360055013478 2201 3 -0.39979277416022185 2201 4 -0.22397918727465793 2201 5 -0.04746569158129432 2201 6 -0.20587840309272565 2201 7 0.021591958198563077 2201 8 0.05158555332934611 2201 9 -0.14518019519670844 2201 10 0.06878838954465749 2201 11 0.010257726560602975 2201 12 0.04203458546673744 2201 13 0.7526979319218938 2201 14 -0.05992124958102352 2201 15 -0.05182330758331233 2201 16 0.3190716430650582 2201 17 0.03132894584597068 2201 18 0.1182745905964282 2201 19 -0.0014375430736208727 2201 20 0.04646287894899359 2201 21 -0.024177626574633848 2201 22 -0.13859639028961368 2201 23 -0.3588576469264808 2201 24 -0.06677591402223726 2201 25 -0.13703866337359472 2201 26 -0.23934894000100826 2201 27 -0.2986552835182792 2201 28 -0.07321610389863764 2201 29 -0.11163088981722069 2201 30 -0.27674439767409353 2201 31 -0.2782222481785297 2201 32 -0.3051971351862264 2201 33 -0.4937696167816341 2201 34 -0.30913705921333445 2201 35 -0.4103188436280547 2201 36 -0.19152423844782618 2201 37 -0.27093072524752415 2201 38 -0.2282931926522167 2201 39 -0.13189453093651338 2201 40 -0.1948174562100462 2201 41 -0.0951013023289562 2201 42 0.19405977972510158 2201 43 0.0016011661664805388 2201 44 -0.18900552779806645 2201 45 -0.282278415327319 2201 46 -0.3551355448334519 2201 47 -0.2361107899634689 2201 48 -0.2614085659103793 2201 49 -0.20600004484199275 2201 50 -0.22926170114553202 2201 51 -0.41880972702762875 1101 1 0.08535447997426715 1101 2 0.06324471092039374 1101 3 0.12116495545061667 1101 4 -0.5111903059173173 1101 5 -0.1615510078106226 1101 6 -0.226645366876582 1101 7 -0.18414319416362446 1101 8 -0.1411132502485728 1101 9 -0.0740160050034504 1101 10 -0.18632881974420457 1101 11 -0.08109432118254034 1101 12 -0.19913890454758842 1101 13 0.24007717374943383 1101 14 -0.14492096744356786 1101 15 -0.13840090338237587 1101 16 0.06151736557308851 1101 17 -0.031984592991022964 1101 18 -0.3224620838878849 1101 19 -0.25214569416826293 1101 20 -0.15050145446939922 1101 21 -0.29882446331226475 1101 22 -0.2399248748376479 1101 23 -0.10216364078554818 1101 24 -0.14255465801230827 1101 25 -0.0410835635486535 1101 26 -0.05158876687320202 1101 27 -0.1443928654738329 1101 28 -0.0789364735504563 1101 29 -0.09795306854040575 1101 30 -0.3322750496390867 1101 31 -0.2271051770509871 1101 32 -0.15606302513785408 1101 33 -0.04335962094175727 1101 34 -0.013964458867540941 1101 35 -0.21855488703732107 1101 36 -0.6541821662547136 1101 37 -0.28653335101741817 1101 38 -0.27622691226661544 1101 39 -0.1566154307547052 1101 40 -0.2573068208818583 1101 41 -0.09815133102151208 1101 42 0.06733591140893935 1101 43 -0.018314174142304296 1101 44 -0.2585996707204927 1101 45 -0.17600738139805117 1101 46 -0.1658604109649744 1101 47 -0.1393072099644712 1101 48 -0.3998649631805904 1101 49 -0.22087384777525187 1101 50 -0.3198559590659116 1101 51 -0.28864805494232826 1102 1 -0.007942342008881553 1102 2 -0.09830131009512044 1102 3 0.031768008172246454 1102 4 -0.004141315569626158 1102 5 -0.13227886049096327 1102 6 -0.10223007937860729 1102 7 -0.3417459561228995 1102 8 -0.20471203351023348 1102 9 -0.036353645734365614 1102 10 -0.24480047434501273 1102 11 -0.12999364451555806 1102 12 -0.5914766082129859 1102 13 0.5594766836490663 1102 14 0.1184391949344672 1102 15 0.008386144812824625 1102 16 -0.7660712123697664 1102 17 -0.4835378608234677 1102 18 -0.6117226029881593 1102 19 -0.4008119716268723 1102 20 -0.24422625619229166 1102 21 -0.24139132241437267 1102 22 -0.3238665242624137 1102 23 -0.15262925863014942 1102 24 -0.5314110770904321 1102 25 -0.3249910501661688 1102 26 -0.306792364718639 1102 27 -0.12054810620870882 1102 28 -0.36593372088728415 1102 29 -0.2386314976866828 1102 30 -0.33725682871697416 1102 31 -0.31296119731659644 1102 32 -0.1865393662343493 1102 33 -0.38234528570565374 1102 34 -0.32341574931125905 1102 35 -0.326944879095187 1102 36 -0.23976724600090904 1102 37 -0.18694245230874884 1102 38 -0.26339116281793196 1102 39 -0.2565432437686326 1102 40 -0.20778189659090174 1102 41 -0.19337202088603078 1102 42 0.04431366346361898 1102 43 -0.20951652083005712 1102 44 -0.17836082451120205 1102 45 -0.3721655466906313 1102 46 -0.3863212628496342 1102 47 -0.32468314756501115 1102 48 -0.3157307664721216 1102 49 -0.37033499845968265 1102 50 -0.3346534223868005 1102 51 -0.6399473120096388 1103 1 -0.29086889164137375 1103 2 -0.08196251040423519 1103 3 -0.022836648908757695 1103 4 0.15023884107441177 1103 5 0.043583057814302606 1103 6 0.10948712825739904 1103 7 0.05503871429083773 1103 8 0.08687459493120286 1103 9 0.013166023251834247 1103 10 0.15172288273202383 1103 11 0.04879697551061213 1103 12 0.1315878761828202 1103 13 0.7219043562365499 1103 14 0.4128854355449647 1103 15 0.29268120801874886 1103 16 0.47267370233247874 1103 17 0.13144319157873952 1103 18 0.15190666515255913 1103 19 0.09747862140032737 1103 20 0.10635461428791615 1103 21 -0.004261820759737134 1103 22 0.13181447967718896 1103 23 0.022787749253211587 1103 24 0.04325221665839507 1103 25 0.06592000924408126 1103 26 0.09773694940270872 1103 27 0.16253227211434051 1103 28 0.14798293641845106 1103 29 0.12923431664998475 1103 30 0.13880251393213427 1103 31 -0.031163132761207635 1103 32 -0.11636285899333387 1103 33 -0.034170186454204554 1103 34 -0.013128206367206019 1103 35 0.11182741668746132 1103 36 0.12036075762864584 1103 37 -0.09417857821819808 1103 38 0.10938501978807125 1103 39 -0.1877215344373795 1103 40 -0.02709243860249444 1103 41 0.002041048688376179 1103 42 0.2536180886689863 1103 43 0.13057993566294357 1103 44 0.04478403455014046 1103 45 0.024632935790812382 1103 46 -0.09151128984965595 1103 47 -0.09566272312764568 1103 48 0.04495113963388775 1103 49 0.0060732467184010375 1103 50 -0.04911326028851448 1103 51 0.0038929924896677903 2103 1 0.048863514419636545 2103 2 7.859154843785632E-4 2103 3 0.12533200268661915 2103 4 0.23116346330531456 2103 5 0.07335438240711767 2103 6 0.19051615800565003 2103 7 0.19486010247701557 2103 8 0.11236070815211008 2103 9 0.10163136237485304 2103 10 0.22607747286812696 2103 11 0.08294020054458429 2103 12 0.26572304526748525 2103 13 0.9008670602347806 2103 14 0.5952611358926347 2103 15 0.39523931988050265 2103 16 -0.14319268448218736 2103 17 0.2276750562034593 2103 18 0.2860581544989742 2103 19 0.28490707979201346 2103 20 0.366066531823769 2103 21 0.33113136860304593 2103 22 0.26714359904675433 2103 23 0.3320050380522872 2103 24 0.3333299450213545 2103 25 0.3073611078892853 2103 26 0.32650412280455754 2103 27 0.1651326826006212 2103 28 0.5243763955341478 2103 29 0.44090782304099463 2103 30 0.4184450529767716 2103 31 0.42024692435791877 2103 32 0.18525011894068655 2103 33 0.25067033700382524 2103 34 0.2998727232689973 2103 35 0.36330710424431345 2103 36 0.45372470851307156 2103 37 0.33425650909621396 2103 38 0.3679978612609247 2103 39 0.44289403038096253 2103 40 0.30765262011979644 2103 41 0.17256195322855916 2103 42 0.7014833885742391 2103 43 0.5417449483737471 2103 44 0.4046676138825305 2103 45 0.44518144920346003 2103 46 0.3186429607661623 2103 47 0.3247964868196078 2103 48 0.5217107403796817 2103 49 0.40532934910948626 2103 50 0.4841981803584332 2103 51 0.3177518193866433 2102 1 -0.20545718921761846 2102 2 -0.0815434673989266 2102 3 -0.13962581309812094 2102 4 0.05080497881724 2102 5 -4.3347717839026245E-4 2102 6 -0.015926519063690137 2102 7 0.013701919667049367 2102 8 0.052562926077598604 2102 9 -0.02275441184688276 2102 10 -0.16373802634893053 2102 11 -0.13023184330076276 2102 12 -0.04119220603933371 2102 13 0.5517388021314673 2102 14 0.17808406108401442 2102 15 0.10604970146732029 2102 16 -0.26046138542482566 2102 17 -0.0911058226183954 2102 18 -0.06750627851127433 2102 19 -0.02999284500704391 2102 20 0.044103227822205326 2102 21 0.11278173303801964 2102 22 0.14990231132023268 2102 23 0.08694837486032725 2102 24 -0.04999628162017444 2102 25 0.013276613080449806 2102 26 -0.07263702118101634 2102 27 -0.03984096722852826 2102 28 -0.6364581407298147 2102 29 -0.34109927518598226 2102 30 -0.16190645267359116 2102 31 -0.21154004230947976 2102 32 0.028303377241350347 2102 33 0.06678594999431908 2102 34 -0.14487240336356422 2102 35 -0.05503163982302084 2102 36 -0.058480249787301375 2102 37 0.007786313650193222 2102 38 0.06497094053133878 2102 39 0.0781252493032838 2102 40 0.02011579541568409 2102 41 -0.17970670063314031 2102 42 0.2171468765562139 2102 43 0.10609833870939411 2102 44 0.1418232099467147 2102 45 0.1134866344729204 2102 46 0.15491394715052564 2102 47 0.04237123610946014 2102 48 -0.12057568065240787 2102 49 -4.1368184021450816E-5 2102 50 -0.029939660281229408 2102 51 -0.02694033342176283 2101 1 0.12994044661776982 2101 2 0.06402954380112291 2101 3 0.13982284379397214 2101 4 0.06690942617144913 2101 5 0.14041062006645433 2101 6 0.13225708325204977 2101 7 0.07833517083716401 2101 8 0.15374293096868996 2101 9 0.17050712895269982 2101 10 0.013133989077488195 2101 11 0.20150233019982977 2101 12 0.2625215595248278 2101 13 -4.539661128105262 2101 14 -1.5848756310793135 2101 15 -0.8548662474909818 2101 16 -0.09886689155455741 2101 17 -0.061282746833065005 2101 18 0.11098945680100059 2101 19 0.10367358437063956 2101 20 -0.2015957791596037 2101 21 0.00917560246616489 2101 22 -0.057366413208903566 2101 23 -0.045455870392792974 2101 24 0.14435381491885835 2101 25 0.05636232015528009 2101 26 -0.025673716378761924 2101 27 0.14327664261320194 2101 28 0.22166517729232282 2101 29 -0.031188816215866666 2101 30 0.12650520536661958 2101 31 0.2799327802801912 2101 32 0.1781627903143601 2101 33 0.21681990082227998 2101 34 0.21557682589909177 2101 35 0.16297231337255624 2101 36 0.2524126678486738 2101 37 0.15532006285013011 2101 38 0.15207723398641093 2101 39 -0.0567641010962916 2101 40 0.10085482265655799 2101 41 0.0714026280816853 2101 42 -2.211493258911055 2101 43 -0.906882079311707 2101 44 -0.347847962534388 2101 45 -0.2061390700348511 2101 46 0.024844442977638437 2101 47 0.08347435203786091 2101 48 0.1022403137381076 2101 49 -0.009664164263213593 2101 50 0.2257220939648903 2101 51 0.26095682389897945 2104 1 0.15630418414139768 2104 2 0.057573517142259334 2104 3 0.1441674260636745 2104 4 0.24019409939319303 2104 5 0.08438097677338874 2104 6 0.11841999889651333 2104 7 0.16236128481588707 2104 8 -0.11130142970016266 2104 9 -0.0070002567979727814 2104 10 0.13514458621589398 2104 11 -0.0021774238167182602 2104 12 0.129940652358016 2104 13 0.8128991201820597 2104 14 0.4850480206477954 2104 15 0.24273408427728072 2104 16 0.4153294628607256 2104 17 0.2774638296377887 2104 18 0.33446209833833507 2104 19 0.19832876831283386 2104 20 0.03333623693842469 2104 21 0.11556652895378505 2104 22 0.21089381255437445 2104 23 0.21736525456915956 2104 24 0.2698019541465868 2104 25 0.060193226719313486 2104 26 0.2717997369453471 2104 27 0.13249562510121393 2104 28 0.26051992982128525 2104 29 0.250361407755193 2104 30 0.42442995642825565 2104 31 0.36081209297866224 2104 32 0.37244609905540216 2104 33 0.4193685220628254 2104 34 0.28906832795484405 2104 35 0.3727434152792526 2104 36 0.3174557665003377 2104 37 0.34122222119537327 2104 38 0.07348021216999001 2104 39 0.2685195613092759 2104 40 0.25837537409325506 2104 41 0.32032572487104716 2104 42 0.7335355505139489 2104 43 0.35468838537151015 2104 44 0.3825391271848062 2104 45 0.4532893939835887 2104 46 0.500427157603383 2104 47 0.34512179565363965 2104 48 0.4286777824638506 2104 49 0.39551182769631055 2104 50 0.2529037288446645 2104 51 0.7917437916260468 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 4.0 9 6.0 10 13.0 11 20.0 12 26.0 13 15.0 14 14.0 15 29.0 16 35.0 17 94.0 18 210.0 19 492.0 20 1092.0 21 2196.0 22 4239.0 23 7470.0 24 12252.0 25 19592.0 26 29514.0 27 42904.0 28 59476.0 29 81350.0 30 107086.0 31 136444.0 32 171279.0 33 210929.0 34 258528.0 35 313780.0 36 374766.0 37 452460.0 38 537428.0 39 12838.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 23.63657106204905 24.895522957399645 13.009546333696425 38.45835964685488 2 16.270497380297837 31.6443169984157 25.655207570237703 26.429978051048764 3 14.643151511220193 27.824261734721578 31.08117445573581 26.451412298322417 4 14.949647145754996 28.1199697382272 32.29270297844378 24.637680137574026 5 15.673158752329389 28.861425476154047 32.08075775704704 23.38465801446953 6 17.118255170891686 28.99496466948107 32.8681005712255 21.018679588401746 7 17.721010709367825 32.06700881553927 29.64370499425012 20.56827548084279 8 16.933372629453338 30.534248613295862 30.937198360562114 21.59518039668869 9 17.98636528046783 29.16827364765059 30.960007501986546 21.885353569895035 10 18.315952085996457 29.69295440780489 30.30245556095329 21.68863794524537 11 18.325964135709807 29.47857668137216 30.994520870540672 21.20093831237736 12 19.830239351444803 29.760112699015927 29.27939329799033 21.130254651548942 13 18.49341919253524 29.689005993833423 30.133801878457945 21.683772935173387 14 18.382123273714633 29.95742763650055 30.43966294646162 21.2207861433232 15 18.820467731939356 30.0077699146367 30.55934924497159 20.612413108452355 16 18.760536448443936 29.74537665401529 30.53238016739865 20.961706730142122 17 18.224421467222793 29.824584906412028 31.208776642156664 20.74221698420852 18 18.827327297155044 29.778714592272408 30.989440389654664 20.404517720917887 19 18.784824103135573 29.393385055297284 30.718538576601308 21.10325226496584 20 18.800655154689693 29.48062139575024 31.01623714738372 20.70248630217635 21 19.334688258509853 29.390830106131887 30.623059933405365 20.65142170195289 22 19.091050427826207 29.252905555306818 30.399157567875612 21.256886448991363 23 18.995448374426992 29.360646612157154 30.520428024137615 21.123476989278245 24 19.33314131420757 29.40920979664187 30.526246393912547 20.731402495238008 25 18.870810010075505 29.3716522208771 30.707238500420576 21.05029926862682 26 18.628458386521196 29.648254850380486 31.14229898514031 20.58098777795801 27 19.01059771687244 29.75219111145583 31.05388406675927 20.18332710491246 28 19.00343191903294 29.436127311715133 30.902608103334117 20.65783266591781 29 19.000402597210307 29.486649777795954 31.037918170530588 20.475029454463154 30 19.60533948315411 29.420933016673985 30.638167325198225 20.335560174973686 31 19.343998522131713 29.208170016901004 30.503349529246776 20.94448193172051 32 19.20317368321971 29.32551123219598 30.755367911617448 20.715947172966864 33 19.327630952217262 29.38658329217141 30.724495735553926 20.561290020057406 34 18.97283420680241 29.2240449949975 30.66095039734441 21.14217040085568 35 18.87329306861573 29.439572954238546 31.054815723689543 20.632318253456173 36 19.167540370770173 29.561986926519314 31.016201893687544 20.254270809022973 37 18.79588021979494 29.491493275778062 31.07103144692226 20.641595057504738 38 18.74176794271193 29.6497903028833 31.090911526713466 20.5175302276913 39 19.13175786915379 29.77216946310736 30.954860462345174 20.141212205393675 40 19.05733731653095 29.403486308522016 30.790331462302166 20.748844912644866 41 19.041473153252753 29.53910727770253 30.83549144710077 20.583928121943945 42 19.6821033201226 29.594702356568575 30.584238354470273 20.138955968838555 43 18.951787750186668 29.347644453782756 30.74157560049383 20.95899219553674 44 18.83593227796157 29.44358894087718 31.082101549213547 20.6383772319477 45 19.15238128141545 29.32268483689172 31.10719168351206 20.41774219818077 46 18.843863139490978 29.19435603941225 31.099004116871633 20.862776704225144 47 18.738062116128066 29.640747694917575 31.12296663773489 20.49822355121947 48 19.067074778704278 29.55434842388936 31.175973285160886 20.202603512245474 49 18.94071131194151 29.311542388753498 30.936872405000454 20.810873894304542 50 18.86867151361914 29.505220938468053 31.102790387931297 20.523317159981513 51 19.303737444604664 29.569987625289535 30.845534703835455 20.280740226270346 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 14.0 1 25.0 2 36.0 3 62.0 4 88.0 5 130.5 6 173.0 7 203.5 8 234.0 9 337.0 10 440.0 11 630.0 12 820.0 13 1157.5 14 1495.0 15 2197.0 16 2899.0 17 3984.0 18 5069.0 19 7125.0 20 9181.0 21 12690.5 22 16200.0 23 21916.5 24 27633.0 25 36693.5 26 59178.5 27 72603.0 28 90020.5 29 107438.0 30 129573.5 31 151709.0 32 175527.0 33 199345.0 34 221305.0 35 243265.0 36 259293.5 37 275322.0 38 283060.0 39 290798.0 40 287659.0 41 284520.0 42 271916.0 43 259312.0 44 242139.5 45 224967.0 46 203757.5 47 182548.0 48 162038.0 49 141528.0 50 122545.5 51 103563.0 52 87752.5 53 71942.0 54 59143.0 55 46344.0 56 37008.5 57 27673.0 58 22051.0 59 16429.0 60 13330.0 61 10231.0 62 8380.0 63 6529.0 64 5123.5 65 3718.0 66 3082.0 67 2446.0 68 1911.5 69 1377.0 70 1132.0 71 887.0 72 770.5 73 654.0 74 577.0 75 442.5 76 385.0 77 315.0 78 245.0 79 191.5 80 138.0 81 108.0 82 78.0 83 55.0 84 32.0 85 20.5 86 9.0 87 7.5 88 6.0 89 4.5 90 3.0 91 2.5 92 2.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18818423017561275 2 0.0 3 0.0 4 0.0 5 0.0 6 0.007896827942925675 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.001868445897210093 18 6.698202273017315E-4 19 1.762684808688767E-4 20 0.0 21 5.993128349541807E-4 22 0.0011633719737345863 23 4.5829805025907945E-4 24 0.002079968074252745 25 0.0 26 0.025453168637465797 27 0.004300950933200591 28 0.009447990574571791 29 0.0 30 0.02562943711833467 31 0.002256236555121622 32 1.4101478469510137E-4 33 0.0078615742467519 34 0.0 35 0.012409301053168918 36 0.0 37 0.004230443540853041 38 0.003877906579115288 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0016216700239936655 45 0.0 46 0.007227007715623944 47 8.460887081706082E-4 48 0.003736891794420186 49 0.012620823230211572 50 8.108350119968328E-4 51 0.005358561818413852 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 2836582.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.09383177109278 #Duplication Level Percentage of deduplicated Percentage of total 1 85.01404273622482 65.5405830888701 2 10.855852700385825 16.73838563630616 3 2.405041627177507 5.562416238242939 4 0.7695141992696909 2.372991928958589 5 0.2920177684773592 1.1256384358581724 6 0.14079989154219072 0.6512881891165057 7 0.0674005357970725 0.3637315897614718 8 0.04276668350589582 0.2637638002888881 9 0.04894519952957761 0.3396035680682244 >10 0.3326169989171841 5.062021637714791 >50 0.025351005826578815 1.2684153447813629 >100 0.005600248114939796 0.6788295841895492 >500 5.0405231440877926E-5 0.032330957843275845 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.007896827942925675 0.0 0.0 2 0.008037842727620778 0.0 0.0 3 0.008355125993184755 0.0 0.0 4 0.010082557105699747 0.0 0.0 5 0.010329332978916174 0.0 0.0 6 0.013431658242208405 0.0 0.0 7 0.07681780396265646 0.0 0.0 8 0.07780490745552217 0.0 0.0 9 0.07953233856803717 0.0 0.0 10 0.08781695716887437 0.0 0.0 11 0.11104914294739232 0.0 0.0 12 0.12631399339063704 0.0 0.0 13 0.13971039793667167 0.0 0.0 14 0.14704316674081694 0.0 0.0 15 0.15564506860721813 0.0 0.0 16 0.1632598669807536 0.0 0.0 17 0.17189702254332856 0.0 0.0 18 0.18074570028294618 0.0 0.0 19 0.19424786591750212 0.0 0.0 20 0.20369585649207392 0.0 0.0 21 0.2150122929638558 0.0 0.0 22 0.2242840150575587 0.0 0.0 23 0.23538892935229794 0.0 0.0 24 0.2457535160273879 0.0 0.0 25 0.26355663259514445 0.0 0.0 26 0.2817122861246387 0.0 0.0 27 0.30152486337430046 0.0 0.0 28 0.31227724070730195 0.0 0.0 29 0.32412248262169047 0.0 0.0 30 0.33575620235903636 0.0 0.0 31 0.35137358976401883 0.0 0.0 32 0.36681470868813243 0.0 0.0 33 0.3799643373609506 0.0 0.0 34 0.3935722640840279 0.0 0.0 35 0.40696866863006254 0.0 0.0 36 0.421175908188094 0.0 0.0 37 0.4354184014422992 0.0 0.0 38 0.4502249538352849 0.0 0.0 39 0.467146727998697 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCCCCG 335 0.0 14.772953 5 CCCGGGT 325 0.0 14.5350895 8 CCCCGGG 330 0.0 13.633201 7 GTGCCCC 390 0.0 12.112789 4 GCCCCGG 405 1.8189894E-12 11.664167 6 CCGGGTA 445 0.0 11.626517 9 CGGGTAA 645 0.0 10.462689 10 ACCCCCC 405 1.7298589E-9 9.999092 42 CCCTACG 315 5.263155E-7 9.999091 40 CACGTGA 345 1.7362072E-7 9.78034 7 CCGATGT 795 0.0 8.772788 40 CGATGTA 830 0.0 8.673911 41 GGGTAAC 660 3.6379788E-12 8.521352 11 ACCGATG 900 0.0 8.498478 39 CCCCCCT 510 1.0002623E-8 8.382034 44 GTCACCG 895 0.0 8.295189 36 GGTAACC 625 8.185452E-11 8.278664 12 TCACGTG 330 9.857667E-5 8.180065 6 GGTGCCC 580 1.3915269E-9 8.144806 3 ACTGCGG 640 1.364242E-10 8.084917 38 >>END_MODULE