FastQCFastQC Report
Sun 19 Apr 2015
HCNNTADXX l01n01 rnh201-frb.34100000008893.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCNNTADXX l01n01 rnh201-frb.34100000008893.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2836582
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCCCG3350.014.7729535
CCCGGGT3250.014.53508958
CCCCGGG3300.013.6332017
GTGCCCC3900.012.1127894
GCCCCGG4051.8189894E-1211.6641676
CCGGGTA4450.011.6265179
CGGGTAA6450.010.46268910
ACCCCCC4051.7298589E-99.99909242
CCCTACG3155.263155E-79.99909140
CACGTGA3451.7362072E-79.780347
CCGATGT7950.08.77278840
CGATGTA8300.08.67391141
GGGTAAC6603.6379788E-128.52135211
ACCGATG9000.08.49847839
CCCCCCT5101.0002623E-88.38203444
GTCACCG8950.08.29518936
GGTAACC6258.185452E-118.27866412
TCACGTG3309.857667E-58.1800656
GGTGCCC5801.3915269E-98.1448063
ACTGCGG6401.364242E-108.08491738