##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename HCNNTADXX l01n01 rad27-frb.341000000088bc.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2812676 Sequences flagged as poor quality 0 Sequence length 51 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.499072769135157 33.0 33.0 33.0 27.0 33.0 2 31.694624620823728 33.0 33.0 33.0 27.0 33.0 3 31.68931970834892 33.0 33.0 33.0 27.0 33.0 4 35.332408354179435 37.0 37.0 37.0 33.0 37.0 5 35.38196471971887 37.0 37.0 37.0 33.0 37.0 6 35.050977076634496 37.0 33.0 37.0 33.0 37.0 7 35.02499754682018 37.0 33.0 37.0 33.0 37.0 8 35.00988204827004 37.0 33.0 37.0 33.0 37.0 9 35.082047132339454 37.0 33.0 37.0 33.0 37.0 10 34.986311256611145 37.0 33.0 37.0 33.0 37.0 11 35.049439039548105 37.0 33.0 37.0 33.0 37.0 12 34.86415036783476 37.0 33.0 37.0 27.0 37.0 13 34.32571223987406 37.0 33.0 37.0 27.0 37.0 14 35.749896895340946 37.0 33.0 40.0 27.0 40.0 15 35.851774253415606 37.0 33.0 40.0 27.0 40.0 16 35.64554929184876 37.0 33.0 40.0 27.0 40.0 17 35.882523973610894 37.0 33.0 40.0 33.0 40.0 18 35.81469959568752 37.0 33.0 40.0 27.0 40.0 19 35.939595602195205 37.0 33.0 40.0 33.0 40.0 20 35.8727905382632 37.0 33.0 40.0 27.0 40.0 21 35.85990743334817 37.0 33.0 40.0 27.0 40.0 22 35.858655600573975 37.0 33.0 40.0 27.0 40.0 23 35.814209670790376 37.0 33.0 40.0 27.0 40.0 24 35.72448479668472 37.0 33.0 40.0 27.0 40.0 25 35.78214838822531 37.0 33.0 40.0 27.0 40.0 26 35.60415241570661 37.0 33.0 40.0 27.0 40.0 27 35.50200485231858 37.0 33.0 40.0 27.0 40.0 28 35.314178739392666 37.0 33.0 40.0 27.0 40.0 29 35.37818113426502 37.0 33.0 40.0 27.0 40.0 30 35.27614769706856 37.0 33.0 40.0 27.0 40.0 31 35.27354803752725 37.0 33.0 40.0 27.0 40.0 32 35.237378923132276 37.0 33.0 40.0 27.0 40.0 33 35.150691370068934 37.0 33.0 40.0 27.0 40.0 34 35.160661235065824 37.0 33.0 40.0 27.0 40.0 35 35.15440207119483 37.0 33.0 40.0 27.0 40.0 36 35.096209446093326 37.0 33.0 40.0 27.0 40.0 37 35.12297790431603 37.0 33.0 40.0 27.0 40.0 38 35.05994504877206 37.0 33.0 40.0 27.0 40.0 39 35.01945122722987 37.0 33.0 40.0 27.0 40.0 40 35.038218052843625 37.0 33.0 40.0 27.0 40.0 41 34.89174153013003 37.0 33.0 40.0 27.0 40.0 42 34.57043719219704 37.0 33.0 40.0 27.0 40.0 43 34.70644254795078 37.0 33.0 40.0 27.0 40.0 44 34.65355696852392 37.0 33.0 40.0 27.0 40.0 45 34.67435922232067 37.0 33.0 40.0 27.0 40.0 46 34.484392798886184 37.0 33.0 40.0 27.0 40.0 47 34.59086506942143 37.0 33.0 40.0 27.0 40.0 48 34.44691638852111 37.0 33.0 40.0 27.0 40.0 49 34.44818884222712 37.0 33.0 40.0 27.0 40.0 50 34.39770062388985 37.0 33.0 40.0 27.0 40.0 51 33.32017623074965 37.0 33.0 37.0 22.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.08987624609671485 2201 2 0.06313573921626059 2201 3 -0.3890237407236654 2201 4 -0.20084376994949338 2201 5 -0.030886540660013395 2201 6 -0.1599037898949902 2201 7 0.0845357103993507 2201 8 0.09676220002764069 2201 9 -0.12381648389197863 2201 10 0.15348259248925444 2201 11 0.04536747863091506 2201 12 0.07926887217453071 2201 13 0.8009747308973729 2201 14 -0.01632547372746984 2201 15 -0.01219235936570584 2201 16 0.3257567166287245 2201 17 0.09222350990979322 2201 18 0.14911639006560762 2201 19 0.06927269737622765 2201 20 0.11132420003152532 2201 21 -0.0044207107781559785 2201 22 -0.0054802058641101326 2201 23 -0.2697420276366884 2201 24 -0.014364487327355846 2201 25 -0.07685667462293111 2201 26 -0.191937667823332 2201 27 -0.25309958593567927 2201 28 -0.019430620292929746 2201 29 -0.06164522859350541 2201 30 -0.1783669412186768 2201 31 -0.21966208210428562 2201 32 -0.21870627381358076 2201 33 -0.38005962145238925 2201 34 -0.236942242516335 2201 35 -0.296976118338371 2201 36 -0.07247310750167202 2201 37 -0.14345583705908638 2201 38 -0.10595683324991256 2201 39 -0.028516514929513903 2201 40 -0.0622889401231248 2201 41 0.023516353187801542 2201 42 0.22250962422354092 2201 43 0.04784312122765044 2201 44 -0.086501356346524 2201 45 -0.11572827367103855 2201 46 -0.23442640300834938 2201 47 -0.07924748228570877 2201 48 -0.1903071890153072 2201 49 -0.04715620538543419 2201 50 -0.15904282507087686 2201 51 -0.3275634389575046 1101 1 0.06009900313626915 1101 2 0.04730391885672702 1101 3 0.09383023384756939 1101 4 -0.5413947670527577 1101 5 -0.16974851077039688 1101 6 -0.2934698116583405 1101 7 -0.1941552505714199 1101 8 -0.19820762726534014 1101 9 -0.13770708769595075 1101 10 -0.14594023881070228 1101 11 -0.08673093296508938 1101 12 -0.18323586219893429 1101 13 0.20988628748706617 1101 14 -0.20237459761569454 1101 15 -0.16944300858605743 1101 16 0.03237345362293098 1101 17 -0.06971748250686716 1101 18 -0.31788545657979483 1101 19 -0.2710942947826709 1101 20 -0.19033579836354875 1101 21 -0.29965398746676186 1101 22 -0.23063747305679527 1101 23 -0.12545142169844326 1101 24 -0.19717604270898903 1101 25 -0.07761310155049728 1101 26 -0.09141110493915505 1101 27 -0.16485442189551947 1101 28 -0.08430035875748132 1101 29 -0.10045520959354803 1101 30 -0.355327422053648 1101 31 -0.24848643098991374 1101 32 -0.12759478219002318 1101 33 -0.050601679510918984 1101 34 -0.044652382663358026 1101 35 -0.20379481230948926 1101 36 -0.6769897243094292 1101 37 -0.3169152548025451 1101 38 -0.26587379805740596 1101 39 -0.17057643174151593 1101 40 -0.2171729917440146 1101 41 -0.10815542629035235 1101 42 0.06037533544170515 1101 43 -0.053524538256603194 1101 44 -0.3213915403888308 1101 45 -0.21183025159267999 1101 46 -0.17163802565039532 1101 47 -0.18882284214537748 1101 48 -0.4990673408862776 1101 49 -0.3338453708416509 1101 50 -0.3899263288770243 1101 51 -0.2743848786300731 1102 1 -0.007577827603537202 1102 2 -0.11002394461077003 1102 3 0.049883497429377144 1102 4 0.04036008079617659 1102 5 -0.13247052767126632 1102 6 -0.1562045223608095 1102 7 -0.353370396661127 1102 8 -0.16284275389404712 1102 9 -0.015959914226705507 1102 10 -0.29284819004035967 1102 11 -0.14707747172888475 1102 12 -0.6169753231154189 1102 13 0.508181439344412 1102 14 0.097240401122761 1102 15 0.02744350307905563 1102 16 -0.7590447713287958 1102 17 -0.49703539835957855 1102 18 -0.5836037195959136 1102 19 -0.37617048865761404 1102 20 -0.25212662518622153 1102 21 -0.21082205019003908 1102 22 -0.3869122383365564 1102 23 -0.21529096449101814 1102 24 -0.5561468694499254 1102 25 -0.30599361518968493 1102 26 -0.2694661783598846 1102 27 -0.10438425242456617 1102 28 -0.32947794518752005 1102 29 -0.2269771884363223 1102 30 -0.3248323320509954 1102 31 -0.2863079184569557 1102 32 -0.2548956993418656 1102 33 -0.3840791116825599 1102 34 -0.3799472178802219 1102 35 -0.2923289952807764 1102 36 -0.19735270787345627 1102 37 -0.1378518962483284 1102 38 -0.27044981486497477 1102 39 -0.2652634049435463 1102 40 -0.23483082929739396 1102 41 -0.1435257603197897 1102 42 0.09100565872110167 1102 43 -0.11953953623349634 1102 44 -0.16169906800471523 1102 45 -0.39526152247463386 1102 46 -0.3397655260529078 1102 47 -0.2899595960293553 1102 48 -0.19130844809223646 1102 49 -0.3635134070790613 1102 50 -0.28683668841475907 1102 51 -0.5874408167395231 1103 1 -0.21695934214011459 1103 2 -0.04874102194160557 1103 3 0.006140227026939726 1103 4 0.16723901821173115 1103 5 0.08928150705835947 1103 6 0.18414723454138482 1103 7 0.1058937893844174 1103 8 0.07555638570140388 1103 9 0.039082958653800404 1103 10 0.20771771911126535 1103 11 0.10215168105729333 1103 12 0.1445193798863187 1103 13 0.7346768549329852 1103 14 0.44513382756067443 1103 15 0.29390653163277847 1103 16 0.5520611228507448 1103 17 0.20887802070230066 1103 18 0.19392046086124282 1103 19 0.13240341455566806 1103 20 0.12994799399713486 1103 21 0.041152132783956574 1103 22 0.16370559913942628 1103 23 0.09681130026304885 1103 24 0.1384166374616811 1103 25 0.12686565535587135 1103 26 0.1594403906495927 1103 27 0.23997044121325217 1103 28 0.2842501402755673 1103 29 0.15535186247915078 1103 30 0.17773449143692943 1103 31 0.009987941562684455 1103 32 -0.10350124960248763 1103 33 -0.023776659673352185 1103 34 -0.012090979105686017 1103 35 0.0950471894425533 1103 36 0.13583782725377347 1103 37 -0.10656636030315525 1103 38 0.22078001183867002 1103 39 -0.15072639773910623 1103 40 0.0020984409972513163 1103 41 0.05193070660138943 1103 42 0.2680137368202722 1103 43 0.15686750267782656 1103 44 0.138644056537359 1103 45 0.10690450716925426 1103 46 -0.11028224446535262 1103 47 -0.08715070891403798 1103 48 0.04664063963131326 1103 49 0.0036381186554663714 1103 50 0.016216124144648347 1103 51 0.02457417319427435 2103 1 -0.04004832635538236 2103 2 -0.07623728638724003 2103 3 0.0648544739588175 2103 4 0.14474508920162066 2103 5 0.001907207909560782 2103 6 0.11531448287597357 2103 7 0.10175997883493437 2103 8 0.04338507435399208 2103 9 0.03861500745565394 2103 10 0.1091098600966589 2103 11 -0.013262362637171066 2103 12 0.18297240396512393 2103 13 0.847516604112208 2103 14 0.47027318360174064 2103 15 0.21881996548774652 2103 16 -0.28295469517311744 2103 17 0.060503230893665716 2103 18 0.11756394361935207 2103 19 0.10135240580341787 2103 20 0.2458863522957273 2103 21 0.16243536067777598 2103 22 0.05595567275720725 2103 23 0.19104743915889344 2103 24 0.13887955523152584 2103 25 0.08587279199217335 2103 26 0.13141273833512912 2103 27 -0.011605712394860745 2103 28 0.2887906237756539 2103 29 0.29664145876971304 2103 30 0.27384702674577 2103 31 0.22438784092593522 2103 32 0.06140022680521895 2103 33 0.07497783400454239 2103 34 0.18293572908484634 2103 35 0.19176759600068038 2103 36 0.28665869256611387 2103 37 0.15471974433123847 2103 38 0.14379500154866776 2103 39 0.24807000405599666 2103 40 0.08228956650999919 2103 41 0.014638012478307871 2103 42 0.5164670650306604 2103 43 0.3933526479108522 2103 44 0.2157824978119507 2103 45 0.25551842856899043 2103 46 0.050392702476486306 2103 47 0.08445107258840778 2103 48 0.2973804292196931 2103 49 0.19129135187498747 2103 50 0.285472273105313 2103 51 0.07892493310817628 2102 1 -0.18315380976960682 2102 2 -0.056914152319553324 2102 3 -0.12747364754677903 2102 4 0.02353212006794081 2102 5 -0.013963010997670722 2102 6 -0.04652065791742643 2102 7 -0.022913219071654112 2102 8 0.02868818162953346 2102 9 -0.0025956871131427306 2102 10 -0.1836215422874048 2102 11 -0.09319791339078165 2102 12 -0.018025634867775864 2102 13 0.5452991576534956 2102 14 0.1805586433652806 2102 15 0.11914202921595773 2102 16 -0.24207776252222857 2102 17 -0.12136259520870141 2102 18 -0.08620625277410454 2102 19 -0.03496364427740417 2102 20 0.07862704741053506 2102 21 0.12884079719452757 2102 22 0.15614842661128847 2102 23 0.12383962425955275 2102 24 -0.03364266721508358 2102 25 0.07595869739390793 2102 26 7.274435729129891E-4 2102 27 -0.0266261685195488 2102 28 -0.6248247256853219 2102 29 -0.291722051255654 2102 30 -0.10274298012804195 2102 31 -0.1363842238868216 2102 32 0.04648420395036368 2102 33 0.11945865503196273 2102 34 -0.05634395413655113 2102 35 -0.05833891661489332 2102 36 -0.034709493743264375 2102 37 0.06083603809940996 2102 38 0.08714370184539177 2102 39 0.08496032978774792 2102 40 0.03170846342456457 2102 41 -0.17030158701577136 2102 42 0.26389932735134636 2102 43 0.14247831967789892 2102 44 0.18452588410876558 2102 45 0.09420077335293087 2102 46 0.20536666428084516 2102 47 0.08910745696518774 2102 48 -0.053975046153901474 2102 49 0.05619094490663201 2102 50 -0.052891331911745 2102 51 -0.016342244868113198 2101 1 0.16975232076421065 2101 2 0.12299451561442609 2101 3 0.18571752712978906 2101 4 0.17151729992179554 2101 5 0.19738486840955005 2101 6 0.23822663315880277 2101 7 0.14272830053423036 2101 8 0.21787983584468407 2101 9 0.2174259967188874 2101 10 0.06990294544297626 2101 11 0.2189467334962174 2101 12 0.2787104614920608 2101 13 -4.424415526380876 2101 14 -1.4533108611969254 2101 15 -0.6920064169704432 2101 16 0.03637682592285785 2101 17 0.0594220433612378 2101 18 0.21003790195321415 2101 19 0.17078701864672752 2101 20 -0.11559973152945702 2101 21 0.08088265327876343 2101 22 0.030817860571247024 2101 23 0.027262408267809235 2101 24 0.25997162961402864 2101 25 0.14599021893671704 2101 26 0.0019229083694796145 2101 27 0.21611231646540574 2101 28 0.2946978647677483 2101 29 0.05161874894746177 2101 30 0.20066794489534345 2101 31 0.3472121923109128 2101 32 0.2747054677749219 2101 33 0.2868811370616555 2101 34 0.27021290803627807 2101 35 0.22563034103977486 2101 36 0.32921678763854345 2101 37 0.2021825659838683 2101 38 0.22865470597998439 2101 39 0.034749554867950394 2101 40 0.1796757615926481 2101 41 0.04807119940177529 2101 42 -2.1214452988273536 2101 43 -0.8181447717495871 2101 44 -0.28879569469503963 2101 45 -0.13601998330791076 2101 46 0.16538365858827575 2101 47 0.22859975129264143 2101 48 0.2563510622228762 2101 49 0.11771792100783784 2101 50 0.3794443611211449 2101 51 0.38665718755137135 2104 1 0.12801173587145342 2104 2 0.0584822315717517 2104 3 0.11607142887793742 2104 4 0.19484492880293658 2104 5 0.05849500672186281 2104 6 0.11841043125541972 2104 7 0.1355210871512753 2104 8 -0.10122129639790245 2104 9 -0.015044789900549915 2104 10 0.08219685399832599 2104 11 -0.026197212462463426 2104 12 0.13276570266409493 2104 13 0.7778804519533438 2104 14 0.4788048768896118 2104 15 0.21432975550668942 2104 16 0.3375091099988694 2104 17 0.2670886712081497 2104 18 0.31705673245042476 2104 19 0.20841289133567642 2104 20 -0.007723438655673931 2104 21 0.10158580449992627 2104 22 0.2164023581782928 2104 23 0.17152364187686686 2104 24 0.2640622443941254 2104 25 0.025776027684479175 2104 26 0.2593114701952075 2104 27 0.10448738349152364 2104 28 0.19029502110431906 2104 29 0.17718760768268282 2104 30 0.30902021237332633 2104 31 0.30925268063846545 2104 32 0.3221081064174882 2104 33 0.3571994462210313 2104 34 0.27682813918102056 2104 35 0.33899371606053563 2104 36 0.22981172596936972 2104 37 0.2870509999985984 2104 38 -0.038092975040441956 2104 39 0.24730286064198026 2104 40 0.21852052864009863 2104 41 0.2838265019566961 2104 42 0.6991745512387197 2104 43 0.2506672547454869 2104 44 0.31943522097702726 2104 45 0.4022163219550947 2104 46 0.434969173831405 2104 47 0.2430223485281715 2104 48 0.33428589307383305 2104 49 0.3756766468612014 2104 50 0.20756441590333452 2104 51 0.7155750853413281 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 5.0 10 11.0 11 26.0 12 20.0 13 17.0 14 23.0 15 28.0 16 48.0 17 111.0 18 236.0 19 541.0 20 1228.0 21 2453.0 22 4394.0 23 7750.0 24 12797.0 25 20307.0 26 30464.0 27 44024.0 28 61340.0 29 83063.0 30 108032.0 31 138184.0 32 171970.0 33 212507.0 34 257986.0 35 311115.0 36 371088.0 37 443658.0 38 516758.0 39 12492.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 21.023415788577562 21.751485882777864 20.294633297024813 36.93046503161976 2 16.207803529450246 29.363211404370787 26.967308001348183 27.461677064830788 3 17.41629679351621 30.196261496169484 25.216235357360752 27.171206352953558 4 19.871076512189813 29.458138797358814 25.26892539346871 25.40185929698266 5 16.971204646393684 30.26971467741041 28.01328699075187 24.74579368544404 6 16.785329516969192 27.136020195914597 31.999466657185017 24.0791836299312 7 15.641758951262071 28.815050151528293 32.27534916926088 23.267841727948756 8 16.887298785924862 29.458885417303666 31.14944629242757 22.504369504343906 9 16.98670589858199 28.50210973464416 31.96905722521897 22.542127141554875 10 18.53043151788546 29.56920740248788 29.425571946431084 22.47478913319558 11 18.282055949565468 30.440441771466038 29.63441221100475 21.643090067963747 12 19.135798079835713 30.53821343091063 29.114480302743722 21.211508186509928 13 17.799775018523285 30.348003111627502 30.203798802279398 21.648423067569816 14 18.06852264533846 30.243582979340673 30.159392692226195 21.528501683094674 15 18.58461479388312 29.789531392879947 30.36425098376066 21.26160282947627 16 18.590658860103332 29.19198656368526 30.37395704304371 21.843397533167703 17 18.199595182148478 29.22439303098922 30.55699252974892 22.019019257113378 18 18.84547012436626 29.388265911983673 30.17015920872057 21.596104754929495 19 18.53523520356103 29.418417375660848 30.321225028176073 21.725122392602046 20 18.462666869557673 29.98767010491077 30.45121443067029 21.098448594861264 21 18.570667724741313 29.92018931572151 30.526662343350914 20.982480616186265 22 18.40150549271608 29.571533302946634 30.517301575676036 21.509659628661247 23 18.38731055353849 29.485847600925524 30.559276814753822 21.567565030782163 24 18.98452940969421 29.493068224119302 30.310560198703918 21.21184216748257 25 18.705353904964525 29.14871816021469 30.449685637449885 21.6962422973709 26 18.604118561835502 29.42726549528254 30.44838888748617 21.520227055395786 27 18.86015546696087 29.4408757291847 30.439930326794922 21.2590384770595 28 18.755364593222495 29.261433739317926 30.474302931779086 21.508898735680493 29 18.505970826358954 29.578451268471735 30.748546935373998 21.167030969795313 30 18.891737463161462 29.679121088589238 30.631704240463765 20.797437207785535 31 18.616923881535744 29.38332784025195 30.632052787623255 21.367695490589053 32 18.689823744300355 29.544650555076572 30.69476565902566 21.07076004159741 33 18.98569492267891 29.56882807310978 30.526470366530273 20.919006637681036 34 18.80223673114145 29.293526876184817 30.36698859022511 21.53724780244863 35 18.713677572047484 29.55955811446241 30.41408634603842 21.31267796745169 36 19.065366931704897 29.480395182381475 30.34263455869073 21.1116033272229 37 18.716145371308947 29.326237112797266 30.545723725963768 21.411893789930016 38 18.648331347010988 29.375965309994285 30.53913718708312 21.436566155911606 39 18.791286305283652 29.562772249629887 30.647717689488584 20.998223755597873 40 18.631936277054308 29.343123772521256 30.63865159015827 21.386288360266168 41 18.548065969916195 29.699581466190917 30.600218439663863 21.15213412422903 42 19.270794076530677 29.557936996653723 30.35472269113115 20.816546235684452 43 18.70528279830311 29.394285015408812 30.586601514003036 21.313830672285043 44 18.829180812684505 29.473777024982677 30.54053698514806 21.156505177184762 45 18.938157114434794 29.605400693147736 30.424513879309238 21.031928313108228 46 18.909843529387086 29.275403852191502 30.244727334659093 21.57002528376232 47 18.74001386592715 29.575311538940145 30.343590564058804 21.341084031073898 48 18.948633045897218 29.460062640285546 30.535521464910882 21.055782848906354 49 18.715962217617083 29.210088766696202 30.57874719626102 21.495201819425695 50 18.66450690344422 29.460466875769292 30.640704473426165 21.234321747360323 51 18.895756171220867 29.5057725585958 30.525281321343655 21.073189948839673 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 25.0 1 52.5 2 80.0 3 115.0 4 150.0 5 198.5 6 247.0 7 305.0 8 363.0 9 460.5 10 558.0 11 794.0 12 1030.0 13 1408.5 14 1787.0 15 2488.5 16 3190.0 17 4323.5 18 5457.0 19 7438.5 20 9420.0 21 12821.0 22 16222.0 23 21767.0 24 27312.0 25 36195.0 26 57413.0 27 69748.0 28 85137.5 29 100527.0 30 120027.5 31 139528.0 32 161959.5 33 184391.0 34 204819.0 35 225247.0 36 242564.5 37 259882.0 38 268784.0 39 277686.0 40 275331.5 41 272977.0 42 263496.5 43 254016.0 44 239012.0 45 224008.0 46 207057.0 47 190106.0 48 170916.5 49 151727.0 50 133513.0 51 115299.0 52 99463.5 53 83628.0 54 69899.5 55 56171.0 56 45764.0 57 35357.0 58 28310.5 59 21264.0 60 17767.5 61 14271.0 62 12314.0 63 10357.0 64 8214.5 65 6072.0 66 5005.5 67 3939.0 68 2982.0 69 2025.0 70 1536.5 71 1048.0 72 904.5 73 761.0 74 685.5 75 526.0 76 442.0 77 371.5 78 301.0 79 239.0 80 177.0 81 143.0 82 109.0 83 80.5 84 52.0 85 36.0 86 20.0 87 14.0 88 8.0 89 4.5 90 1.0 91 1.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18679719953524687 2 0.0 3 0.0 4 0.0 5 0.0 6 0.00803505273981077 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0018843265274777472 18 5.688532913140368E-4 19 2.1331998424276385E-4 20 0.0 21 7.110666141425461E-4 22 0.001493239889699347 23 2.844266456570184E-4 24 0.001742113204649238 25 0.0 26 0.02399849822731093 27 0.0041597396927338945 28 0.009030545999610335 29 0.0 30 0.02641612471539559 31 0.0016710065432349833 32 3.55533307071273E-5 33 0.008817226015367571 34 0.0 35 0.012941412377394339 36 0.0 37 0.0035197797400056035 38 0.003839759716369749 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0016710065432349833 45 0.0 46 0.007075112810718333 47 9.243865983853099E-4 48 0.0038753130470768763 49 0.012799199054565828 50 7.821732755568008E-4 51 0.005155232952533459 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 2812676.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.06379237862639 #Duplication Level Percentage of deduplicated Percentage of total 1 85.6353065165363 64.28110868637313 2 10.651668640475625 15.991092866291762 3 2.2050567450644754 4.965597650838284 4 0.546534743270803 1.6409988198634171 5 0.20565914405789304 0.771877764516385 6 0.1218007520181885 0.5485695816632317 7 0.09266954205422277 0.48692890852063964 8 0.058298865293002676 0.3500907136210769 9 0.04633518121985151 0.31302849806216665 >10 0.3668540753690721 5.976504487169796 >50 0.05323758524910144 2.7011255897236284 >100 0.016402764152483925 1.896852512038384 >500 1.7544523896453616E-4 0.07622392131809083 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.029864797793986937 0.0 0.0 2 0.030149224439643955 0.0 0.0 3 0.03061141773883661 0.0 0.0 4 0.03608663066773422 0.0 0.0 5 0.03729544391177654 0.0 0.0 6 0.04237957020289575 0.0 0.0 7 0.12429444415211706 0.0 0.0 8 0.12664096397878746 0.0 3.55533307071273E-5 9 0.13016074371879308 0.0 3.55533307071273E-5 10 0.14431096934022974 0.0 3.55533307071273E-5 11 0.17961542673240716 0.0 3.55533307071273E-5 12 0.20485829153446752 0.0 3.55533307071273E-5 13 0.22683024991147221 0.0 3.55533307071273E-5 14 0.23820731573775294 0.0 3.55533307071273E-5 15 0.2492288482569624 0.0 3.55533307071273E-5 16 0.25914822752425093 0.0 3.55533307071273E-5 17 0.2684987535002254 0.0 3.55533307071273E-5 18 0.27952028601943485 0.0 3.55533307071273E-5 19 0.2969414180659273 0.0 3.55533307071273E-5 20 0.30518979078998076 0.0 3.55533307071273E-5 21 0.3153935967029263 0.0 3.55533307071273E-5 22 0.32477967600960794 0.0 3.55533307071273E-5 23 0.3361211885051815 0.0 3.55533307071273E-5 24 0.34671608105590546 0.0 3.55533307071273E-5 25 0.3649549397086618 0.0 3.55533307071273E-5 26 0.3847225915818246 0.0 3.55533307071273E-5 27 0.4049168834234729 0.0 3.55533307071273E-5 28 0.41810716911581713 0.0 3.55533307071273E-5 29 0.42941312828068356 0.0 3.55533307071273E-5 30 0.44288784061868486 0.0 3.55533307071273E-5 31 0.458851286106185 0.0 3.55533307071273E-5 32 0.47744567806601257 0.0 3.55533307071273E-5 33 0.49330246356139135 0.0 3.55533307071273E-5 34 0.509976975663034 0.0 3.55533307071273E-5 35 0.5269003610796267 0.0 3.55533307071273E-5 36 0.5446414731024832 0.0 3.55533307071273E-5 37 0.5622759251332183 0.0 3.55533307071273E-5 38 0.5812969570615314 0.0 3.55533307071273E-5 39 0.6012779289189369 0.0 3.55533307071273E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGATCT 855 0.0 11.315128 44 AGTGATC 930 0.0 11.128382 43 TGATCTC 1125 0.0 9.599439 45 CACACAG 1175 0.0 9.190298 38 GTCACAC 1200 0.0 8.998834 36 TACGGCC 365 3.9009938E-6 8.629172 42 CACAGTG 1190 0.0 8.5073 40 ACACAGT 1250 0.0 8.458452 39 GTGCCCC 520 1.4146281E-8 8.220673 4 CTACGGC 415 2.4901474E-6 8.131476 41 CTCGCCC 250 0.0046648034 8.11323 1 CAGTGAT 1255 0.0 8.066827 42 CCCCGGG 475 2.4195288E-7 8.051016 7 TGCCCCG 520 1.2320015E-7 7.788006 5 CAGTCAC 1515 0.0 7.722595 34 ACAGTGA 1400 0.0 7.713286 41 CCTACGG 380 6.005673E-5 7.696371 40 CCGGCTA 325 7.3434354E-4 7.627823 1 AGTCACA 1455 0.0 7.5771456 35 TCCAGTC 1550 0.0 7.5483484 32 >>END_MODULE