FastQCFastQC Report
Sun 19 Apr 2015
HCNNTADXX l01n01 rad27-frb.341000000088bc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCNNTADXX l01n01 rad27-frb.341000000088bc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2812676
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCT8550.011.31512844
AGTGATC9300.011.12838243
TGATCTC11250.09.59943945
CACACAG11750.09.19029838
GTCACAC12000.08.99883436
TACGGCC3653.9009938E-68.62917242
CACAGTG11900.08.507340
ACACAGT12500.08.45845239
GTGCCCC5201.4146281E-88.2206734
CTACGGC4152.4901474E-68.13147641
CTCGCCC2500.00466480348.113231
CAGTGAT12550.08.06682742
CCCCGGG4752.4195288E-78.0510167
TGCCCCG5201.2320015E-77.7880065
CAGTCAC15150.07.72259534
ACAGTGA14000.07.71328641
CCTACGG3806.005673E-57.69637140
CCGGCTA3257.3434354E-47.6278231
AGTCACA14550.07.577145635
TCCAGTC15500.07.548348432
Produced by FastQC (version 0.11.2)