FastQCFastQC Report
Sun 19 Apr 2015
HCNNTADXX l01n01 dna2-frb rnh201-frb.3410000000890e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCNNTADXX l01n01 dna2-frb rnh201-frb.3410000000890e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2224612
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACAC5700.014.20885136
CAGTCAC8500.010.8521534
AGTCACA8350.010.777657535
CCAGTCA9000.010.74945833
TCCAGTC9450.010.47566132
GAGACCC3704.1600288E-910.33940829
GAACTCC10100.010.02695128
GTGCCCC3053.765912E-69.5888194
CACTTGA9750.09.22968140
TCACACT10400.09.08546637
CTCCAGT11150.08.87867531
ACTCCAG11250.08.80133630
GAATCTC8800.08.69274345
TGCCCCG2607.0128636E-48.6526315
CGTCTGA11800.08.58219823
CACACGT11050.08.55141319
ACGTCTG11900.08.51007822
CTGAACT11650.08.50086526
ACACGTC11300.08.3633520
GCACACG11450.08.25267318
Produced by FastQC (version 0.11.2)