##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename HCNNTADXX l01n01 dna2-frb rnh201-frb.3410000000890e.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2224612 Sequences flagged as poor quality 0 Sequence length 51 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.51692654719115 33.0 33.0 33.0 27.0 33.0 2 31.69950670049429 33.0 33.0 33.0 27.0 33.0 3 31.704494087058777 33.0 33.0 33.0 27.0 33.0 4 35.36046780292474 37.0 37.0 37.0 33.0 37.0 5 35.42729159062344 37.0 37.0 37.0 33.0 37.0 6 35.07137514317103 37.0 33.0 37.0 33.0 37.0 7 35.02934624105237 37.0 33.0 37.0 33.0 37.0 8 34.99054576708208 37.0 33.0 37.0 33.0 37.0 9 35.068776038248465 37.0 33.0 37.0 33.0 37.0 10 34.961532168306206 37.0 33.0 37.0 33.0 37.0 11 35.03745911646615 37.0 33.0 37.0 33.0 37.0 12 34.85947347222797 37.0 33.0 37.0 27.0 37.0 13 34.29612444776887 37.0 33.0 37.0 27.0 37.0 14 35.73209800180886 37.0 33.0 40.0 27.0 40.0 15 35.837979386967255 37.0 33.0 40.0 27.0 40.0 16 35.637901800403846 37.0 33.0 40.0 27.0 40.0 17 35.87102739713712 37.0 33.0 40.0 33.0 40.0 18 35.808346803847144 37.0 33.0 40.0 27.0 40.0 19 35.94780932585098 37.0 33.0 40.0 33.0 40.0 20 35.87348535385047 37.0 33.0 40.0 27.0 40.0 21 35.86305072525007 37.0 33.0 40.0 27.0 40.0 22 35.85342342844505 37.0 33.0 40.0 27.0 40.0 23 35.80246712685178 37.0 33.0 40.0 27.0 40.0 24 35.70687607546844 37.0 33.0 40.0 27.0 40.0 25 35.77245560124642 37.0 33.0 40.0 27.0 40.0 26 35.60470275265979 37.0 33.0 40.0 27.0 40.0 27 35.50158499549585 37.0 33.0 40.0 27.0 40.0 28 35.31602454720194 37.0 33.0 40.0 27.0 40.0 29 35.38034632556149 37.0 33.0 40.0 27.0 40.0 30 35.27721688096621 37.0 33.0 40.0 27.0 40.0 31 35.27542825445516 37.0 33.0 40.0 27.0 40.0 32 35.2382222158291 37.0 33.0 40.0 27.0 40.0 33 35.1464237359144 37.0 33.0 40.0 27.0 40.0 34 35.16793220570598 37.0 33.0 40.0 27.0 40.0 35 35.162817605946564 37.0 33.0 40.0 27.0 40.0 36 35.106005901253795 37.0 33.0 40.0 27.0 40.0 37 35.14692135077937 37.0 33.0 40.0 27.0 40.0 38 35.07912480918021 37.0 33.0 40.0 27.0 40.0 39 35.03954442392651 37.0 33.0 40.0 27.0 40.0 40 35.05757138772964 37.0 33.0 40.0 27.0 40.0 41 34.89876077266508 37.0 33.0 40.0 27.0 40.0 42 34.57046667014293 37.0 33.0 40.0 27.0 40.0 43 34.71723968044765 37.0 33.0 40.0 27.0 40.0 44 34.680510578923425 37.0 33.0 40.0 27.0 40.0 45 34.70315902278689 37.0 33.0 40.0 27.0 40.0 46 34.52097399456624 37.0 33.0 40.0 27.0 40.0 47 34.63146786945319 37.0 33.0 40.0 27.0 40.0 48 34.49424349055026 37.0 33.0 40.0 27.0 40.0 49 34.49410503944058 37.0 33.0 40.0 27.0 40.0 50 34.444782730651454 37.0 33.0 40.0 27.0 40.0 51 33.35860590520954 37.0 33.0 37.0 22.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.0848550520598863 2201 2 0.09281410844628724 2201 3 -0.3684768412933188 2201 4 -0.24531978428169765 2201 5 -0.06708265519751677 2201 6 -0.13471489250250812 2201 7 0.02713486509411922 2201 8 0.06251147790605671 2201 9 -0.1700669324119346 2201 10 0.14014223864840858 2201 11 0.06700184357023886 2201 12 0.09786781099167996 2201 13 0.8179773117720828 2201 14 -0.024466157023717017 2201 15 0.013559489662334556 2201 16 0.3384330771239874 2201 17 0.005798624267555397 2201 18 0.07853082174408144 2201 19 -0.004802828068164899 2201 20 0.025792510795909607 2201 21 -0.025734134342002335 2201 22 -0.07471493813515906 2201 23 -0.33511756852578856 2201 24 -0.050649535386718014 2201 25 -0.12648711078409747 2201 26 -0.2521372323716733 2201 27 -0.2754768749495753 2201 28 -0.11791684221971366 2201 29 -0.12818617034928792 2201 30 -0.24661866596179038 2201 31 -0.28372462797043596 2201 32 -0.22776419717537522 2201 33 -0.4891675383248071 2201 34 -0.2906900061729445 2201 35 -0.34599813921511213 2201 36 -0.1392942163704447 2201 37 -0.21370876416915507 2201 38 -0.15087765255496777 2201 39 -0.09606712150193886 2201 40 -0.08543117597101713 2201 41 4.420259873683108E-4 2201 42 0.19322921567285078 2201 43 0.038558297753716886 2201 44 -0.22725594552817086 2201 45 -0.20776441417950053 2201 46 -0.3233662982006891 2201 47 -0.11295037974407052 2201 48 -0.17751567886018194 2201 49 -0.15454943531556609 2201 50 -0.22040947601393412 2201 51 -0.3486945706525688 1101 1 0.09246400318830439 1101 2 0.0490180317846729 1101 3 0.10095452071836775 1101 4 -0.5014479940357077 1101 5 -0.1489788659525928 1101 6 -0.23568751497781193 1101 7 -0.1412662712345636 1101 8 -0.08224829762561825 1101 9 -0.007378463731875229 1101 10 -0.13749477727191817 1101 11 -0.10627565962766994 1101 12 -0.18140851529491187 1101 13 0.25761478106499425 1101 14 -0.1654189542405291 1101 15 -0.1434734825567432 1101 16 0.07137091814330176 1101 17 0.016981093228430666 1101 18 -0.332876145086594 1101 19 -0.16816989017130624 1101 20 -0.08264743664309293 1101 21 -0.2460358070231834 1101 22 -0.17937303495608603 1101 23 -0.07195836737932382 1101 24 -0.1546956665891912 1101 25 -0.03697398444933242 1101 26 -0.0601106093778796 1101 27 -0.05656251028985082 1101 28 -0.06313198157874922 1101 29 -0.05356882416023723 1101 30 -0.32268237627116747 1101 31 -0.2084203538713112 1101 32 -0.12812289912146468 1101 33 0.024713386112821922 1101 34 0.06284656446055692 1101 35 -0.14524451884434342 1101 36 -0.5969530333530244 1101 37 -0.1897309727676486 1101 38 -0.25135665805838414 1101 39 -0.08437474598201078 1101 40 -0.2074941997398838 1101 41 -0.017034959072887546 1101 42 0.13072942406842003 1101 43 -0.016176590697902782 1101 44 -0.18427951269480047 1101 45 -0.14052476014863657 1101 46 -0.13862941775457926 1101 47 -0.16888891292523311 1101 48 -0.43377827971215055 1101 49 -0.2885911834782604 1101 50 -0.2746017037333104 1101 51 -0.21498466972767005 1102 1 0.028209524224067195 1102 2 -0.07290725214293658 1102 3 0.034836730522279424 1102 4 0.0525729901301446 1102 5 -0.07818219074274424 1102 6 -0.05434010321085481 1102 7 -0.3277030178214062 1102 8 -0.09729541285117449 1102 9 -0.010450145104016428 1102 10 -0.2249241167835052 1102 11 -0.0899727009944229 1102 12 -0.6084951392988671 1102 13 0.5498107522328297 1102 14 0.14993061346193315 1102 15 0.06488382226970657 1102 16 -0.7198306793904621 1102 17 -0.4587989376797026 1102 18 -0.5410756402222461 1102 19 -0.32260975480446064 1102 20 -0.16105063289283805 1102 21 -0.15294168491351456 1102 22 -0.2833319961820919 1102 23 -0.10534745369789533 1102 24 -0.4969526283710053 1102 25 -0.2507238882889169 1102 26 -0.1937264875544713 1102 27 -0.0840098171269048 1102 28 -0.29601749659873633 1102 29 -0.1433748300758637 1102 30 -0.2375649558546229 1102 31 -0.23838835134745295 1102 32 -0.20895636249829863 1102 33 -0.2983777547073174 1102 34 -0.26405851374667577 1102 35 -0.26984749560767796 1102 36 -0.17042713223388262 1102 37 -0.13935710191891815 1102 38 -0.18926761452750895 1102 39 -0.1574115683065287 1102 40 -0.18251255255145793 1102 41 -0.1503620498958611 1102 42 0.11900202814143768 1102 43 -0.13530940150968718 1102 44 -0.12530086803351992 1102 45 -0.3364622560056105 1102 46 -0.2594783964190839 1102 47 -0.16844697612485504 1102 48 -0.14583013417894364 1102 49 -0.22168620259254368 1102 50 -0.22259375499308476 1102 51 -0.5372750296787956 1103 1 -0.24228783222087813 1103 2 -0.041751709832958284 1103 3 -8.054456225217166E-4 1103 4 0.15651481175467552 1103 5 0.06269159625648513 1103 6 0.13600369986252758 1103 7 0.054712039182774674 1103 8 0.04745368794382898 1103 9 0.0019703702503477416 1103 10 0.1516524577152154 1103 11 0.07280204462609419 1103 12 0.12588147678172135 1103 13 0.7654297490743289 1103 14 0.4272840394175006 1103 15 0.3255000642442596 1103 16 0.5236229887263519 1103 17 0.21457990954336026 1103 18 0.1721531533651941 1103 19 0.06623112081891946 1103 20 0.11160067857291267 1103 21 0.05621976776748738 1103 22 0.1980678006810166 1103 23 0.06250863854612021 1103 24 0.11357456409648137 1103 25 0.053110623151951586 1103 26 0.11585186014897175 1103 27 0.17366597808740636 1103 28 0.1689809982870898 1103 29 0.15575703649421513 1103 30 0.15273756703437869 1103 31 0.01964781098158852 1103 32 -0.1080724256614829 1103 33 -0.028638964713238124 1103 34 0.0067909426819099394 1103 35 0.08350132115224795 1103 36 0.10279215400683483 1103 37 -0.10488509706207338 1103 38 0.18373304380666866 1103 39 -0.18120612327717822 1103 40 0.026115205483172588 1103 41 0.021190681009535695 1103 42 0.25579339487035924 1103 43 0.1819059700717247 1103 44 0.060546486723936255 1103 45 0.05943187893834079 1103 46 -0.10717020368925745 1103 47 -0.09462276069262288 1103 48 0.04919270451271274 1103 49 0.03116299430944025 1103 50 0.012303072378827551 1103 51 -0.03983278953015201 2103 1 -0.05716123477073509 2103 2 -0.05676017090198826 2103 3 0.04248480755382289 2103 4 0.1249837974749397 2103 5 0.006215352358708515 2103 6 0.04102603485775802 2103 7 0.09466151557389679 2103 8 0.0235450591380868 2103 9 -6.523523673180875E-4 2103 10 0.1128382121267677 2103 11 -0.041752687556552814 2103 12 0.16199725841530466 2103 13 0.7857660467975549 2103 14 0.39138771047909415 2103 15 0.21999265218695285 2103 16 -0.35573269877919955 2103 17 0.002873322281764956 2103 18 0.08129966023194157 2103 19 0.08407197539462885 2103 20 0.22584494473331063 2103 21 0.08290028206933897 2103 22 0.054014002138899286 2103 23 0.17063517753740598 2103 24 0.13819749673301374 2103 25 0.11479844326446198 2103 26 0.09204611429282039 2103 27 -0.03768270332511747 2103 28 0.28250005757581675 2103 29 0.2408845000015063 2103 30 0.22396500663230512 2103 31 0.20741383358011234 2103 32 -0.003449049170662022 2103 33 0.058951078165868864 2103 34 0.15440740649015794 2103 35 0.21095017506480218 2103 36 0.2342522443922732 2103 37 0.09941503906355109 2103 38 0.1297210418011332 2103 39 0.2641288134991058 2103 40 0.10788843738884424 2103 41 -0.07615367846851484 2103 42 0.5087132446836762 2103 43 0.3349949098105398 2103 44 0.21920475387349114 2103 45 0.27377475962381226 2103 46 0.13870427122493112 2103 47 0.07283163134608373 2103 48 0.2525317878468982 2103 49 0.1875654145680059 2103 50 0.2664557004137791 2103 51 0.10228053837795414 2102 1 -0.15218484486532446 2102 2 -0.09119127839042918 2102 3 -0.14536486040185181 2102 4 0.05526077437378518 2102 5 0.015112465617754367 2102 6 -0.007813072206026561 2102 7 -0.010507800876098372 2102 8 0.06112886506533499 2102 9 0.012287615505790939 2102 10 -0.1903736614825604 2102 11 -0.0962722303772452 2102 12 -0.02464980942527717 2102 13 0.5237591165368158 2102 14 0.20717897424041354 2102 15 0.09999637331281974 2102 16 -0.2283093806930907 2102 17 -0.05028684134163086 2102 18 -0.02977473429776012 2102 19 -0.019372440966073157 2102 20 0.09412473243174446 2102 21 0.17807989200933605 2102 22 0.14651208535445193 2102 23 0.08692578680584973 2102 24 -0.03648750339444007 2102 25 0.07461711930367443 2102 26 -0.008621653222924408 2102 27 -0.02934797090752994 2102 28 -0.6177402067038429 2102 29 -0.30975559860448243 2102 30 -0.11677111591971823 2102 31 -0.15061957853297514 2102 32 0.11219912318104974 2102 33 0.15933553607298023 2102 34 -0.11780594335867534 2102 35 -0.0020045286684649 2102 36 0.006752048412415945 2102 37 0.02211826651229387 2102 38 0.09016399817944176 2102 39 0.04087569821183479 2102 40 -0.015004705316513878 2102 41 -0.18472638908431094 2102 42 0.24134753296870315 2102 43 0.14694940487883912 2102 44 0.1872305055725505 2102 45 0.09212372229814036 2102 46 0.19471901097526967 2102 47 0.11226076126600049 2102 48 -0.04350217059644024 2102 49 0.013086568799963061 2102 50 -0.04158605817791994 2102 51 -0.07253246790309476 2101 1 0.10816047428688691 2101 2 0.06791569457928048 2101 3 0.1759971704111578 2101 4 0.12107983047459214 2101 5 0.14721827644557806 2101 6 0.1757686509189682 2101 7 0.1451850431691426 2101 8 0.12127805550647963 2101 9 0.18525528485807286 2101 10 0.021799343854766562 2101 11 0.1903167207097809 2101 12 0.3002031729823429 2101 13 -4.458032437074397 2101 14 -1.4667013442230399 2101 15 -0.7572675528847839 2101 16 -0.049636651188485814 2101 17 -0.0016598804656737798 2101 18 0.2483678016871238 2101 19 0.13157998778941504 2101 20 -0.14384488873473344 2101 21 0.03514995246887054 2101 22 -0.035250365371616965 2101 23 0.022248913692450856 2101 24 0.23430690932525522 2101 25 0.12650209028170423 2101 26 0.04545259939406776 2101 27 0.21190311897051828 2101 28 0.33116300791506603 2101 29 0.02852752326148078 2101 30 0.19811799643044736 2101 31 0.3786348600188205 2101 32 0.26640101010581674 2101 33 0.24206797333041408 2101 34 0.17139301814723495 2101 35 0.19140819379197183 2101 36 0.32604527458433097 2101 37 0.2672065954790952 2101 38 0.1849789613252142 2101 39 -0.018816213804299764 2101 40 0.1332096182236242 2101 41 0.07596070477288919 2101 42 -2.135430601344268 2101 43 -0.8447345509452973 2101 44 -0.2511419379422577 2101 45 -0.08382561131551824 2101 46 0.07907346043406704 2101 47 0.08892849670498748 2101 48 0.17854197553320006 2101 49 0.07758644321812369 2101 50 0.30581792368093375 2101 51 0.3825466499742518 2104 1 0.13794485809777512 2104 2 0.05286257645811787 2104 3 0.16037391811205737 2104 4 0.23635557410929664 2104 5 0.06300602121432064 2104 6 0.07975719725797603 2104 7 0.1577836269121562 2104 8 -0.13637343508295885 2104 9 -0.010965376999088505 2104 10 0.12636030319281133 2104 11 0.004152669649805318 2104 12 0.1286037448480215 2104 13 0.7576746795957945 2104 14 0.48080511788829483 2104 15 0.17680863376544664 2104 16 0.4200824260576397 2104 17 0.27051271016588174 2104 18 0.32337508257829484 2104 19 0.23307183000702025 2104 20 -0.06981990826320583 2104 21 0.0723617319637242 2104 22 0.17407644647058618 2104 23 0.17010487302118804 2104 24 0.25270636358659715 2104 25 0.04515670752056167 2104 26 0.2612454086910674 2104 27 0.09751077954101106 2104 28 0.312162463323034 2104 29 0.20971636343266198 2104 30 0.3488165439101678 2104 31 0.27545640714165387 2104 32 0.2977648003404241 2104 33 0.33111628406329174 2104 34 0.27711653149842874 2104 35 0.277234992326612 2104 36 0.23683266056149677 2104 37 0.2589420348628835 2104 38 0.0029048800284030563 2104 39 0.23287126116098023 2104 40 0.22322937248323882 2104 41 0.33068366475180966 2104 42 0.6866157609388139 2104 43 0.2938119606381093 2104 44 0.32099651802878526 2104 45 0.3432466807890222 2104 46 0.4161475734293134 2104 47 0.27088814016970986 2104 48 0.32035979545494797 2104 49 0.35542540049081595 2104 50 0.17461429644470883 2104 51 0.7284923391400753 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 3.0 10 10.0 11 13.0 12 17.0 13 12.0 14 14.0 15 16.0 16 23.0 17 77.0 18 182.0 19 427.0 20 931.0 21 1934.0 22 3471.0 23 6061.0 24 10230.0 25 16270.0 26 23643.0 27 34992.0 28 48314.0 29 65268.0 30 85170.0 31 109373.0 32 135879.0 33 167090.0 34 203310.0 35 246082.0 36 293752.0 37 352175.0 38 410228.0 39 9645.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 21.939291805280902 26.65149284550129 16.028887304654692 35.38032804456312 2 16.4341467186188 32.23380077065124 24.94408013622151 26.387972374508458 3 15.518481425075473 29.785688470618698 29.735612322508377 24.960217781797457 4 15.651538335673818 29.171154340622095 31.12205634061131 24.055250983092783 5 16.471141933964216 29.71196774988178 30.773501176834433 23.043389139319576 6 17.650634122350482 29.561282010130313 31.848177474237023 20.939906393282183 7 17.46093251317533 32.383489795074375 29.14935278601392 21.00622490573637 8 17.00323472138063 30.71461450356287 30.3369756164221 21.945175158634406 9 18.263004964461217 29.349207861865352 30.19811994181457 22.18966723185886 10 18.560854656901967 29.91883528453501 29.509954994399024 22.010355064163996 11 18.309889544783537 29.886919606655006 30.470077478679432 21.333113369882028 12 19.609217247771745 29.892089047438386 29.25894493062161 21.23974877416826 13 18.421279755750668 29.83347208412074 30.058814750617184 21.68643340951141 14 18.36706805501364 30.141166189879403 30.012244831907765 21.479520923199193 15 18.886978942844866 30.192995452690173 30.085785746008742 20.834239858456215 16 18.85699618630125 29.8137832574849 30.092438591538663 21.23678196467519 17 18.38827743324085 29.853400486567256 30.81152081936056 20.946801260831336 18 18.905432157813255 30.02120826897731 30.452476036184418 20.620883537025016 19 18.690635555239894 29.563212541906296 30.455505379379538 21.29064652347427 20 18.701373542891975 29.738489228683473 30.64687235347108 20.913264874953473 21 19.181948051831295 29.702346756858084 30.337022083051263 20.778683108259365 22 18.934394689145257 29.578850081993153 30.15783681924106 21.328918409620528 23 18.868268027445733 29.596225492311458 30.273738114759997 21.261768365482816 24 19.27129222392842 29.643553265173285 30.32889981335702 20.756254697541276 25 18.701912962799806 29.607634949375445 30.5461806373426 21.14427145048215 26 18.592695274018272 29.752970571480088 30.787112078907697 20.867222075593947 27 18.988342136032838 29.861088322041695 30.648858376260446 20.50171116566502 28 18.91078176882761 29.551206950654702 30.657095100356685 20.880916180160998 29 18.94096588528696 29.616490426195668 30.726751451489072 20.7157922370283 30 19.411137594721623 29.61492650460855 30.439020924750125 20.534914975919698 31 19.088547051578274 29.42807181580343 30.31309280853015 21.170288324088144 32 19.019108967414514 29.46781683081529 30.539600199585543 20.973474002184652 33 19.17556343182264 29.60968421204062 30.469554990909923 20.74519736522682 34 18.822877877130935 29.46868937144994 30.435914217850126 21.272518533569 35 18.737881144060463 29.667094212486226 30.673782539676118 20.921242103777193 36 19.15947589961755 29.743478862830912 30.546809960568407 20.550235276983134 37 18.77714888343158 29.615844001835885 30.73409816595708 20.872908948775454 38 18.698489304295993 29.726576934176897 30.68843957711669 20.886494184410427 39 19.06543702901899 29.939243337714622 30.5244689860524 20.470850647213986 40 18.928559227406847 29.51795638969852 30.486439882550304 21.067044500344327 41 18.89367674003377 29.7748101691441 30.547708993748124 20.783804097074007 42 19.48987059316411 29.800477566425066 30.224866178911196 20.484785661499625 43 18.810606074227778 29.514809773569507 30.600122628125714 21.074461524077005 44 18.75383501253501 29.666510681394914 30.739167901737368 20.840486404332704 45 19.059683216668795 29.62444686983618 30.66835924646635 20.647510667028676 46 18.763272931940094 29.43682309170978 30.691873773855317 21.108030202494817 47 18.661244841181897 29.734346434330117 30.72702418289401 20.877384541593973 48 18.98542014028277 29.705008795207206 30.772795949844166 20.536775114665858 49 18.809532265830576 29.36215533833052 30.6050526026168 21.223259793222105 50 18.819049023261293 29.6273437391132 30.71455189411835 20.839055343507162 51 19.145138001039346 29.723899021612283 30.52121038842406 20.609752588924312 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 50.0 1 89.5 2 129.0 3 175.0 4 221.0 5 267.5 6 314.0 7 364.0 8 414.0 9 531.0 10 648.0 11 829.5 12 1011.0 13 1328.0 14 1645.0 15 2260.5 16 2876.0 17 3751.5 18 4627.0 19 6320.0 20 8013.0 21 10952.5 22 13892.0 23 18565.0 24 23238.0 25 30408.0 26 47845.0 27 58112.0 28 71508.5 29 84905.0 30 100964.5 31 117024.0 32 135313.0 33 153602.0 34 170144.5 35 186687.0 36 199169.0 37 211651.0 38 217177.0 39 222703.0 40 220119.0 41 217535.0 42 208960.5 43 200386.0 44 187526.0 45 174666.0 46 159725.0 47 144784.0 48 129205.5 49 113627.0 50 98908.0 51 84189.0 52 71455.5 53 58722.0 54 48174.5 55 37627.0 56 30379.0 57 23131.0 58 18597.5 59 14064.0 60 11740.5 61 9417.0 62 7948.0 63 6479.0 64 5140.0 65 3801.0 66 3134.0 67 2467.0 68 1935.5 69 1404.0 70 1144.5 71 885.0 72 768.5 73 652.0 74 571.5 75 438.0 76 385.0 77 318.5 78 252.0 79 202.5 80 153.0 81 125.0 82 97.0 83 69.5 84 42.0 85 26.0 86 10.0 87 7.5 88 5.0 89 2.5 90 0.0 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18524578668100325 2 0.0 3 0.0 4 0.0 5 0.0 6 0.00894538013819938 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0021576796313244736 18 3.5961327188741227E-4 19 2.697099539155592E-4 20 0.0 21 4.4951658985926534E-4 22 0.0016182597234933552 23 1.7980663594370614E-4 24 0.0019778729953807673 25 0.0 26 0.024498654147329962 27 0.004719924193522286 28 0.011058108110537928 29 0.0 30 0.026746237096626286 31 0.001438453087549649 32 8.990331797185307E-5 33 0.00934994506907272 34 0.0 35 0.012991029446932768 36 0.0 37 0.003281471105972637 38 0.003326422764958564 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.001932921336394841 45 0.0 46 0.007192265437748245 47 0.0010338881566763103 48 0.004719924193522286 49 0.013170836082876475 50 6.742748847888981E-4 51 0.006113425622086008 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 2224612.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.74419144675176 #Duplication Level Percentage of deduplicated Percentage of total 1 88.55299753906361 71.5014018647789 2 8.9899664176159 14.517751390476946 3 1.3016721230906476 3.153073893231932 4 0.41749449745917205 1.3484102252323522 5 0.16110332760626508 0.6504078963474518 6 0.06983407595761663 0.3383217599177281 7 0.06218678428202676 0.35148551308680753 8 0.04477738368152885 0.2892410912372819 9 0.03293929043345466 0.2393690735591119 >10 0.3372756569215751 5.578243247524313 >50 0.023650161485395685 1.2434942956287185 >100 0.006037004139868629 0.7614105573061174 >500 6.573826286602228E-5 0.027389191672311936 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.01712658207363801 0.0 0.0 2 0.017306388709581715 0.0 0.0 3 0.017576098663497276 0.0 0.0 4 0.01932921336394841 0.0 0.0 5 0.020048439907723235 0.0 0.0 6 0.025262832350090712 0.0 0.0 7 0.13116894092093362 0.0 0.0 8 0.13256244234949735 0.0 0.0 9 0.13463021866284997 0.0 0.0 10 0.14460948695772566 0.0 0.0 11 0.16982736764883044 0.0 0.0 12 0.18358257529852398 0.0 0.0 13 0.1934719402754278 0.0 0.0 14 0.19927070428461233 0.0 0.0 15 0.20592354981452946 0.0 0.0 16 0.21253144368546065 0.0 0.0 17 0.21931914419233556 0.0 0.0 18 0.22601694138123862 0.0 0.0 19 0.23572649972219875 0.0 0.0 20 0.2414353604134114 0.0 0.0 21 0.24939180405392042 0.0 0.0 22 0.25604464958383755 0.0 0.0 23 0.2660239178787132 0.0 0.0 24 0.2761380411505467 0.0 0.0 25 0.2925903483393958 0.0 0.0 26 0.3124140299521894 0.0 0.0 27 0.3334064546986171 0.0 0.0 28 0.34545349930684544 0.0 0.0 29 0.35808491548189075 0.0 0.0 30 0.37053652502099244 0.0 0.0 31 0.3903152549748001 0.0 0.0 32 0.4109480664493404 0.0 0.0 33 0.4268609537303584 0.0 0.0 34 0.4435380192141371 0.0 0.0 35 0.4621480060343107 0.0 0.0 36 0.47954429806186427 0.0 0.0 37 0.49887351142581265 0.0 0.0 38 0.5183825314257048 0.0 0.0 39 0.539689617785034 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCACAC 570 0.0 14.208851 36 CAGTCAC 850 0.0 10.85215 34 AGTCACA 835 0.0 10.7776575 35 CCAGTCA 900 0.0 10.749458 33 TCCAGTC 945 0.0 10.475661 32 GAGACCC 370 4.1600288E-9 10.339408 29 GAACTCC 1010 0.0 10.026951 28 GTGCCCC 305 3.765912E-6 9.588819 4 CACTTGA 975 0.0 9.229681 40 TCACACT 1040 0.0 9.085466 37 CTCCAGT 1115 0.0 8.878675 31 ACTCCAG 1125 0.0 8.801336 30 GAATCTC 880 0.0 8.692743 45 TGCCCCG 260 7.0128636E-4 8.652631 5 CGTCTGA 1180 0.0 8.582198 23 CACACGT 1105 0.0 8.551413 19 ACGTCTG 1190 0.0 8.510078 22 CTGAACT 1165 0.0 8.500865 26 ACACGTC 1130 0.0 8.36335 20 GCACACG 1145 0.0 8.252673 18 >>END_MODULE