##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename HCNNTADXX l01n01 dna2-frb rad27-frb.34100000008942.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1617584 Sequences flagged as poor quality 0 Sequence length 51 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.472782248093452 33.0 33.0 33.0 27.0 33.0 2 31.654686866338935 33.0 33.0 33.0 27.0 33.0 3 31.642114412605466 33.0 33.0 33.0 27.0 33.0 4 35.27822171831571 37.0 37.0 37.0 33.0 37.0 5 35.334801160248865 37.0 37.0 37.0 33.0 37.0 6 34.99590747682964 37.0 33.0 37.0 33.0 37.0 7 34.967968896823905 37.0 33.0 37.0 33.0 37.0 8 34.95043904984223 37.0 33.0 37.0 33.0 37.0 9 35.02963493704191 37.0 33.0 37.0 33.0 37.0 10 34.92453622192109 37.0 33.0 37.0 33.0 37.0 11 35.01160681609116 37.0 33.0 37.0 33.0 37.0 12 34.81732880641747 37.0 33.0 37.0 27.0 37.0 13 34.27374714388866 37.0 33.0 37.0 27.0 37.0 14 35.68297720551143 37.0 33.0 40.0 27.0 40.0 15 35.794335873747514 37.0 33.0 40.0 27.0 40.0 16 35.59416945271467 37.0 33.0 40.0 27.0 40.0 17 35.82825806882363 37.0 33.0 40.0 27.0 40.0 18 35.74959878435988 37.0 33.0 40.0 27.0 40.0 19 35.88667852797753 37.0 33.0 40.0 27.0 40.0 20 35.8190999663696 37.0 33.0 40.0 27.0 40.0 21 35.81100950553418 37.0 33.0 40.0 27.0 40.0 22 35.8064935113107 37.0 33.0 40.0 27.0 40.0 23 35.74944237826289 37.0 33.0 40.0 27.0 40.0 24 35.663512374009635 37.0 33.0 40.0 27.0 40.0 25 35.731513788464774 37.0 33.0 40.0 27.0 40.0 26 35.55401759661322 37.0 33.0 40.0 27.0 40.0 27 35.4449413446226 37.0 33.0 40.0 27.0 40.0 28 35.26011570341942 37.0 33.0 40.0 27.0 40.0 29 35.319519728187224 37.0 33.0 40.0 27.0 40.0 30 35.2157031721382 37.0 33.0 40.0 27.0 40.0 31 35.22282923174314 37.0 33.0 40.0 27.0 40.0 32 35.180505618255374 37.0 33.0 40.0 27.0 40.0 33 35.079580411280034 37.0 33.0 40.0 27.0 40.0 34 35.104345122108036 37.0 33.0 40.0 27.0 40.0 35 35.101600287836675 37.0 33.0 40.0 27.0 40.0 36 35.03225983936537 37.0 33.0 40.0 27.0 40.0 37 35.062173587275836 37.0 33.0 40.0 27.0 40.0 38 35.00389036983551 37.0 33.0 40.0 27.0 40.0 39 34.96809933827239 37.0 33.0 40.0 27.0 40.0 40 34.98549688918783 37.0 33.0 40.0 27.0 40.0 41 34.825759898713144 37.0 33.0 40.0 27.0 40.0 42 34.50207346264553 37.0 33.0 40.0 27.0 40.0 43 34.6439331744132 37.0 33.0 40.0 27.0 40.0 44 34.59944893124561 37.0 33.0 40.0 27.0 40.0 45 34.61837901462923 37.0 33.0 40.0 27.0 40.0 46 34.431497838752115 37.0 33.0 40.0 27.0 40.0 47 34.528972838504835 37.0 33.0 40.0 27.0 40.0 48 34.38771278647662 37.0 33.0 40.0 27.0 40.0 49 34.39260155886804 37.0 33.0 40.0 27.0 40.0 50 34.34291264008546 37.0 33.0 40.0 27.0 40.0 51 33.25265581261932 37.0 33.0 37.0 22.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.03952288117637437 2201 2 0.06400270407717201 2201 3 -0.420823648138132 2201 4 -0.24573017002564512 2201 5 -0.08037393446406327 2201 6 -0.17586172986708704 2201 7 0.025423830378535683 2201 8 0.06373352926399178 2201 9 -0.1821826338892123 2201 10 0.09538875788830126 2201 11 0.06402705204096293 2201 12 0.03719067361025452 2201 13 0.7716265146382284 2201 14 -0.0796844862016215 2201 15 -0.04336816833173884 2201 16 0.33984287723692574 2201 17 0.05986389781240575 2201 18 0.11904364003483892 2201 19 -0.03748291247049451 2201 20 0.04698756605456822 2201 21 0.04067351275688935 2201 22 -0.07351486468617452 2201 23 -0.3121613770360412 2201 24 -0.023909741294687592 2201 25 -0.1527106646815426 2201 26 -0.282482273229256 2201 27 -0.29961212769225654 2201 28 -0.07851608760493178 2201 29 -0.10907438386942658 2201 30 -0.18276737363158446 2201 31 -0.2639638794856509 2201 32 -0.1976900119485805 2201 33 -0.43263153847446745 2201 34 -0.285651852263868 2201 35 -0.3991684432256335 2201 36 -0.12518005961375422 2201 37 -0.20590623035929667 2201 38 -0.23369052069060103 2201 39 -0.09516957301521245 2201 40 -0.11710288503631716 2201 41 -0.001027567112849681 2201 42 0.2583326685186762 2201 43 0.09476205932191561 2201 44 -0.12575631505347928 2201 45 -0.1712665080223914 2201 46 -0.2544015602332621 2201 47 -0.08780357768361569 2201 48 -0.24966584360805655 2201 49 -0.1811751232686163 2201 50 -0.20155408365864957 2201 51 -0.37956445502052816 1101 1 0.08038291837931055 1101 2 0.0922692772733491 1101 3 0.13539405263847115 1101 4 -0.575740256884778 1101 5 -0.16753915405487874 1101 6 -0.2659221485106471 1101 7 -0.21888424529650052 1101 8 -0.12445170434986608 1101 9 -0.13035696672938002 1101 10 -0.14397314545150408 1101 11 -0.08481316912148174 1101 12 -0.17076416166591457 1101 13 0.2751300450389209 1101 14 -0.12907617832009777 1101 15 -0.11950417876466446 1101 16 0.04420799115470686 1101 17 -0.03682615006281509 1101 18 -0.2972010002019587 1101 19 -0.16087571096933573 1101 20 -0.09557255956740107 1101 21 -0.2800833981372506 1101 22 -0.19412117298094955 1101 23 -0.0905466668952215 1101 24 -0.163464047118822 1101 25 -0.05106014230031519 1101 26 -0.02285059488433916 1101 27 -0.12557574015382045 1101 28 -0.11038517753853228 1101 29 -0.12178786754352444 1101 30 -0.3444298589511732 1101 31 -0.26647923363173476 1101 32 -0.09371593024859948 1101 33 -0.007374349503145083 1101 34 0.10181355729385899 1101 35 -0.1601702472999733 1101 36 -0.5886693990004375 1101 37 -0.27261293075339665 1101 38 -0.26114720567714045 1101 39 -0.10836876977170817 1101 40 -0.1727916327478809 1101 41 -0.09298477225130597 1101 42 0.06872384029997392 1101 43 -0.07798220983654147 1101 44 -0.28443214647213466 1101 45 -0.18196874090249082 1101 46 -0.16613080260232493 1101 47 -0.1455635959034396 1101 48 -0.37984409830600896 1101 49 -0.32439483338905717 1101 50 -0.31453000352104254 1101 51 -0.3202093470644627 1102 1 0.016034503011198353 1102 2 -0.10464556987825802 1102 3 0.03688557783641144 1102 4 0.07460832056246858 1102 5 -0.08598000214072243 1102 6 -0.11989056232210515 1102 7 -0.3446350546745407 1102 8 -0.16477818895586438 1102 9 0.0076892149687282085 1102 10 -0.28998199368931665 1102 11 -0.133735016019358 1102 12 -0.5864192913162682 1102 13 0.5628112193503441 1102 14 0.17027670902589875 1102 15 -0.008643895047242722 1102 16 -0.8272702877129277 1102 17 -0.47779327895926826 1102 18 -0.600130182105687 1102 19 -0.35754823131588154 1102 20 -0.2309014019683815 1102 21 -0.17696056990819642 1102 22 -0.3574838536563192 1102 23 -0.1756211992432668 1102 24 -0.5434858994316727 1102 25 -0.32907241566243783 1102 26 -0.26832691572750633 1102 27 -0.09459144493099814 1102 28 -0.31661913074848513 1102 29 -0.2321447561718486 1102 30 -0.32369799395939225 1102 31 -0.2798420043510603 1102 32 -0.2785432734176183 1102 33 -0.37654840337238227 1102 34 -0.38116334050908307 1102 35 -0.2525834612637894 1102 36 -0.18661731683248917 1102 37 -0.07909691261090046 1102 38 -0.22466834892462373 1102 39 -0.2573159819304536 1102 40 -0.20185567016373795 1102 41 -0.1669133890983332 1102 42 0.0721607023986337 1102 43 -0.2017682574875579 1102 44 -0.20466863398790736 1102 45 -0.4753249692495416 1102 46 -0.39577448630822687 1102 47 -0.3918667349469871 1102 48 -0.28237482457470264 1102 49 -0.3753728008649375 1102 50 -0.3111713686565878 1102 51 -0.6504971756740403 1103 1 -0.25595671424497723 1103 2 -0.06594672332963114 1103 3 -0.016746548599709143 1103 4 0.11116193734935109 1103 5 0.043943989787386784 1103 6 0.2083401812171104 1103 7 0.12356288662208925 1103 8 0.05420346821681221 1103 9 0.005742418908702973 1103 10 0.16293756147111083 1103 11 0.080606989693365 1103 12 0.17803837204122885 1103 13 0.7711473187026456 1103 14 0.45754149112452325 1103 15 0.2907693061319634 1103 16 0.5204382298203427 1103 17 0.17087354711602387 1103 18 0.1955862106659083 1103 19 0.0787264095177207 1103 20 0.07390631906324074 1103 21 -0.0390714818512663 1103 22 0.1325517107070553 1103 23 0.03446281381599903 1103 24 0.09565571724522925 1103 25 0.08819213033626738 1103 26 0.13327861472140512 1103 27 0.19856455959330788 1103 28 0.24276340877879932 1103 29 0.1467689509573873 1103 30 0.13704147649161058 1103 31 0.04234070970568382 1103 32 -0.1406179766930009 1103 33 -0.05296836815716688 1103 34 -0.025110746283985463 1103 35 0.08716242886169567 1103 36 0.012034937298047055 1103 37 -0.13045626477148886 1103 38 0.2022344893823984 1103 39 -0.18304097953853926 1103 40 -0.008696231520929132 1103 41 0.01237301929718626 1103 42 0.24127118843494344 1103 43 0.1461813224562576 1103 44 0.05941703706349699 1103 45 0.08345735905952978 1103 46 -0.0787837317208897 1103 47 -0.08360922450489028 1103 48 0.07990232407409081 1103 49 0.06370992588363578 1103 50 -0.015797418693431098 1103 51 0.07449253888029261 2103 1 0.02270246639657003 2103 2 -0.0014447327409605748 2103 3 0.11262780169894882 2103 4 0.2121026362955405 2103 5 -0.009663476965137363 2103 6 0.1058489481720315 2103 7 0.16569875642434795 2103 8 0.08499078816756622 2103 9 0.12231900054931089 2103 10 0.19969066730496365 2103 11 -0.0019501189721182755 2103 12 0.22362538871595206 2103 13 0.8745923884215543 2103 14 0.525779869279738 2103 15 0.3605199399548482 2103 16 -0.12707916730538216 2103 17 0.15298177386554102 2103 18 0.19970816313894346 2103 19 0.19350730752122303 2103 20 0.2895431357402387 2103 21 0.21691380242688751 2103 22 0.16676435838953552 2103 23 0.2895873625865377 2103 24 0.27427459853449676 2103 25 0.24722388359242586 2103 26 0.2962691032809417 2103 27 0.0745569744013963 2103 28 0.4905904828694929 2103 29 0.45030620828499934 2103 30 0.3791106222440561 2103 31 0.32624590385414365 2103 32 0.1564094893556458 2103 33 0.1915700636744262 2103 34 0.25681663530306764 2103 35 0.24370718193567598 2103 36 0.33821829433053097 2103 37 0.2314043030811419 2103 38 0.2366987158569458 2103 39 0.3566781093708329 2103 40 0.21016812861432754 2103 41 0.07020203393166469 2103 42 0.6427608948293297 2103 43 0.46475534653968253 2103 44 0.3649447379992523 2103 45 0.40713141312996726 2103 46 0.2232660446161603 2103 47 0.23558036001659843 2103 48 0.4263178837986743 2103 49 0.34994734695273877 2103 50 0.37139631257145567 2103 51 0.2784309359238222 2102 1 -0.1623906226995686 2102 2 -0.09371186267343035 2102 3 -0.19508906162321082 2102 4 0.05582125562256124 2102 5 0.05911244844801189 2102 6 -0.05915489706993071 2102 7 -0.020668276023663168 2102 8 0.06173012010692247 2102 9 0.01710732642204249 2102 10 -0.17282193785823097 2102 11 -0.10260168458545138 2102 12 -0.04528158957421624 2102 13 0.5221684700829314 2102 14 0.15720948159871284 2102 15 0.1416688672022559 2102 16 -0.25929888087895137 2102 17 -0.14509056951069965 2102 18 -0.09132944155304301 2102 19 -0.05578483045496796 2102 20 0.07531375659861794 2102 21 0.09821490635147256 2102 22 0.15042023399795568 2102 23 0.07365671983660604 2102 24 -0.04737027806271499 2102 25 0.06178372696979295 2102 26 -0.014250682984041418 2102 27 -0.022939749348090288 2102 28 -0.6191760032296045 2102 29 -0.35980225685141676 2102 30 -0.14106299969174074 2102 31 -0.16474587539355667 2102 32 -0.05044088776696043 2102 33 0.050713532403136696 2102 34 -0.02108535213996987 2102 35 -0.03343241528498453 2102 36 -0.027413374209395158 2102 37 0.0394409513818772 2102 38 0.12241818806769089 2102 39 0.09170363149097938 2102 40 0.023190125156084207 2102 41 -0.15677849301347635 2102 42 0.24031321917838966 2102 43 0.1943717756258465 2102 44 0.21802529901412981 2102 45 0.17052615745288335 2102 46 0.18080191212089147 2102 47 0.11817819313623801 2102 48 0.015180893981188603 2102 49 0.0963860079794685 2102 50 -0.0035962002604250642 2102 51 0.02647013580098445 2101 1 0.14227340948474776 2101 2 0.08326013359506845 2101 3 0.2256121484403657 2101 4 0.12676353663973572 2101 5 0.14249143579642265 2101 6 0.20210650625029558 2101 7 0.13652646092696585 2101 8 0.20760622211908242 2101 9 0.2421907803112333 2101 10 0.06666066860841369 2101 11 0.20701976975045255 2101 12 0.25687473626486934 2101 13 -4.573173402060309 2101 14 -1.545570720015725 2101 15 -0.806042335923209 2101 16 -0.07770930387051322 2101 17 0.030885730380447285 2101 18 0.1604458538632585 2101 19 0.15903142740652498 2101 20 -0.13865351287208227 2101 21 0.07048789457150662 2101 22 -0.002512387137244332 2101 23 -7.856031475057534E-4 2101 24 0.15509503024953375 2101 25 0.11707099803625454 2101 26 -0.041258620583775496 2101 27 0.1819985500038257 2101 28 0.2287557949955712 2101 29 0.04096201549139522 2101 30 0.1880518726417293 2101 31 0.3016798806055476 2101 32 0.2387557891598604 2101 33 0.2618380777015261 2101 34 0.11801109550079758 2101 35 0.18826681267792367 2101 36 0.39483830947042264 2101 37 0.15303409973331839 2101 38 0.15048533632563732 2101 39 -0.04339130643033684 2101 40 0.07456274853358735 2101 41 0.017239371533271708 2101 42 -2.166388095578732 2101 43 -0.910718268345093 2101 44 -0.32186317470974046 2101 45 -0.20382447591527608 2101 46 0.03131807642785844 2101 47 0.08520087835189116 2101 48 0.05390121005065396 2101 49 0.06520527214691896 2101 50 0.2755650752565728 2101 51 0.3210197113002877 2104 1 0.11743115849635188 2104 2 0.02621677367670827 2104 3 0.12213967774685486 2104 4 0.2410127404407092 2104 5 0.0980086935930018 2104 6 0.10453370213031832 2104 7 0.13297564164274434 2104 8 -0.18303423456860912 2104 9 -0.08250914054142555 2104 10 0.08209942172626938 2104 11 -0.028553822786371086 2104 12 0.10673587192407297 2104 13 0.7956974458257235 2104 14 0.4435238335085998 2104 15 0.18460046477777325 2104 16 0.3868685415558204 2104 17 0.24510504935835797 2104 18 0.3138767561577538 2104 19 0.18042654076519682 2104 20 -0.020623303048772357 2104 21 0.06982533378992173 2104 22 0.17789597536611268 2104 23 0.18140795008287824 2104 24 0.2532046198785949 2104 25 0.01857248370953357 2104 26 0.1996213694065574 2104 27 0.08759897812664974 2104 28 0.1625867124776832 2104 29 0.1847720897023919 2104 30 0.2877542548564733 2104 31 0.3047644986966418 2104 32 0.36584280155928184 2104 33 0.36540098572805135 2104 34 0.23637000309914669 2104 35 0.3262181435990641 2104 36 0.18278860855711798 2104 37 0.26419298429871674 2104 38 0.007669345659728322 2104 39 0.23890486982444514 2104 40 0.19252541716483051 2104 41 0.31788979671382833 2104 42 0.6428255819187711 2104 43 0.29039823172549006 2104 44 0.2943331961463471 2104 45 0.37126976444735504 2104 46 0.4597045476997934 2104 47 0.26988370153419083 2104 48 0.3365824545841605 2104 49 0.30569420455983476 2104 50 0.1996876869621147 2104 51 0.6498576558536584 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 3.0 9 3.0 10 7.0 11 17.0 12 11.0 13 8.0 14 5.0 15 15.0 16 32.0 17 57.0 18 141.0 19 335.0 20 669.0 21 1364.0 22 2680.0 23 4731.0 24 7733.0 25 12125.0 26 17913.0 27 26147.0 28 36195.0 29 48477.0 30 63260.0 31 80884.0 32 100405.0 33 124053.0 34 149666.0 35 179972.0 36 213450.0 37 252309.0 38 288029.0 39 6888.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 21.943819452314905 23.971580869852797 23.08063420340908 31.003965474423218 2 17.62152691915845 29.775702529204047 24.733985993926744 27.868784557710757 3 18.028986439035005 31.44720768751422 24.444047418866653 26.079758454584123 4 19.531659561419996 30.31898188903946 24.888352011394772 25.26100653814578 5 17.59550045005391 30.960061424939912 27.648579610085168 23.79585851492102 6 17.77624726966076 28.485112270807612 30.920516138445585 22.81812432108604 7 16.55784181841561 30.11942501904074 31.0456829444406 22.27705021810305 8 17.696824399845696 30.094078576444872 30.0394909939762 22.16960602973323 9 17.821021968565464 29.25912966498185 30.799946092444042 22.119902274008645 10 18.5972413179161 29.876717376037348 29.130666475434968 22.395374830611576 11 18.294258598007893 30.61732806457037 29.23860522853836 21.849808108883373 12 19.3047161692994 30.709811669749453 28.82428362298341 21.161188537967735 13 18.09161069842432 30.53090287737762 30.006231515643083 21.37125490855498 14 18.11961542646317 30.48911215739028 29.955600451043036 21.435671965103513 15 18.744559787930644 30.421727712440283 29.88531043828327 20.94840206134581 16 18.845945558314128 29.89266708869524 29.832206550015332 21.4291808029753 17 18.340071961126156 30.11832783115101 30.056876491462344 21.484723716260497 18 19.01341144207313 30.28492050440969 29.930253577270655 20.771414476246527 19 18.564460490362144 30.035794211105475 30.07375214827087 21.325993150261503 20 18.412768672291516 30.64434366314207 30.057109862609916 20.885777801956497 21 18.697746015653014 30.465139282137514 30.08753817430977 20.749576527899702 22 18.46142718220322 30.325102577909448 30.141429852907265 21.07204038698007 23 18.43038268919169 30.2377194560513 30.082672932821207 21.249224921935806 24 19.050230286544465 30.392569008685978 29.918889678835274 20.638311025934282 25 18.676000751738396 29.936126964658406 30.187674952274502 21.2001973313287 26 18.48954516925912 30.14194330306487 30.143303683229295 21.22520784444671 27 18.807152559117792 30.130677697742986 30.289132984356637 20.773036758782585 28 18.749254213039414 29.887555420085953 30.211528367108336 21.151661999766294 29 18.407266639630464 30.18433664032285 30.43545188379707 20.972944836249617 30 18.849789876993324 30.320663291176814 30.3248682248007 20.504678607029163 31 18.622187024937112 29.82178592425948 30.34115195273837 21.214875098065036 32 18.5036059995722 30.057147025622193 30.431409350499695 21.007837624305907 33 18.873285937126145 30.16367333589295 30.153657440295678 20.809383286685225 34 18.61838396027656 29.92988308489698 30.15880473595189 21.292928218874568 35 18.597100839946457 30.142670331063183 30.230096484096048 21.03013234489432 36 19.06367768227183 30.12529797525198 30.14174225264345 20.669282089832738 37 18.54361627348415 29.72667535070512 30.45661679835947 21.273091577451257 38 18.4515021798857 29.89862233202021 30.30238958996387 21.34748589813022 39 18.7791793192811 30.230578442912392 30.32293840690808 20.667303830898426 40 18.63705377896913 29.839810482794093 30.28974074916666 21.23339498907012 41 18.62264958110367 30.05500796249221 30.323309330458265 20.999033125945854 42 19.20425770779137 30.281518610470926 29.993372832570053 20.520850849167648 43 18.60781263909633 29.766862184591343 30.47946814508551 21.145857031226818 44 18.49306388006036 29.920119786318416 30.34452785606848 21.24228847755274 45 18.834261466483348 30.209373979960237 30.19243513783519 20.763929415721226 46 18.85044310269571 29.798733568352315 30.15985577368933 21.19096755526264 47 18.47543118983604 30.044777346606832 30.331567503874666 21.14822395968246 48 18.887372148693295 30.005366292757234 30.336988347478584 20.770273211070883 49 18.615239745190166 29.746816268209425 30.296538253935573 21.341405732664835 50 18.557577998121875 29.849879850714434 30.43625287545338 21.156289275710307 51 18.745390237101784 29.96894573138274 30.26168306346063 21.023980968054843 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 8.0 1 28.0 2 48.0 3 53.5 4 59.0 5 90.0 6 121.0 7 132.5 8 144.0 9 210.0 10 276.0 11 402.0 12 528.0 13 742.0 14 956.0 15 1403.0 16 1850.0 17 2587.0 18 3324.0 19 4515.5 20 5707.0 21 7754.5 22 9802.0 23 13292.5 24 16783.0 25 22277.5 26 35285.0 27 42798.0 28 52326.5 29 61855.0 30 73711.0 31 85567.0 32 98121.5 33 110676.0 34 121553.0 35 132430.0 36 141451.5 37 150473.0 38 155305.0 39 160137.0 40 157846.0 41 155555.0 42 150493.0 43 145431.0 44 136201.5 45 126972.0 46 116428.5 47 105885.0 48 94798.0 49 83711.0 50 73367.0 51 63023.0 52 53905.0 53 44787.0 54 37200.0 55 29613.0 56 23864.0 57 18115.0 58 14599.0 59 11083.0 60 9319.0 61 7555.0 62 6728.5 63 5902.0 64 4564.0 65 3226.0 66 2627.5 67 2029.0 68 1561.5 69 1094.0 70 863.5 71 633.0 72 563.0 73 493.0 74 451.0 75 356.0 76 303.0 77 250.5 78 198.0 79 162.5 80 127.0 81 102.0 82 77.0 83 55.0 84 33.0 85 21.5 86 10.0 87 8.0 88 6.0 89 3.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17909425414692529 2 0.0 3 0.0 4 0.0 5 0.0 6 0.006862085678394445 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.002720106034678879 18 6.182059169724725E-4 19 2.4728236678898903E-4 20 0.0 21 2.4728236678898903E-4 22 0.002040079526009159 23 3.0910295848623624E-4 24 0.0021019001177064065 25 0.0 26 0.024048210170229182 27 0.005749315027843995 28 0.010200397630045797 29 0.0 30 0.026953777979999805 31 0.002163720709403654 32 1.2364118339449451E-4 33 0.009582191713073325 34 0.0 35 0.013538709581697148 36 0.0 37 0.0035237737267430932 38 0.004203800235412813 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0022873618927981483 45 0.0 46 0.007789394553853154 47 0.0015455147924311814 48 0.00494564733577978 49 0.013662350765091641 50 8.036676920642143E-4 51 0.005749315027843995 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1617584.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.81767180499472 #Duplication Level Percentage of deduplicated Percentage of total 1 92.77972707579184 80.54919895423077 2 5.509275924045752 9.56605018113926 3 0.7354334706088014 1.9154586505716946 4 0.27466504141454756 0.9538311768733388 5 0.1420206639323747 0.6164951695404178 6 0.09200948324011568 0.4792829471332515 7 0.07906052429165247 0.48046954554784477 8 0.04927435005337352 0.34223074810705645 9 0.05366494114305698 0.4193158722833051 >10 0.2786462278740296 4.325219359369296 >50 0.005712450186808907 0.3040014385232645 >100 5.098474176547049E-4 0.048445956680470935 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.03443406957536672 0.0 0.0 2 0.03449589016706397 0.0 0.0 3 0.0348049931255502 0.0 0.0 4 0.037957843302109814 0.0 0.0 5 0.03863786981077953 0.0 0.0 6 0.0482818821155501 0.0 0.0 7 0.21464109437284246 0.0 0.0 8 0.21705209744903511 0.0 0.0 9 0.22125589768444792 0.0 0.0 10 0.2403584605188973 0.0 0.0 11 0.2903094986102731 0.0 0.0 12 0.3221471033343554 0.0 0.0 13 0.3481117518471993 0.0 0.0 14 0.3629486938545386 0.0 0.0 15 0.3776001740867862 0.0 0.0 16 0.3915716278103641 0.0 0.0 17 0.40770680224334566 0.0 0.0 18 0.42619115916082256 0.0 0.0 19 0.4525267312238499 0.0 0.0 20 0.4699601380824736 0.0 0.0 21 0.48986636860898725 0.0 0.0 22 0.5079179813845834 0.0 0.0 23 0.528318776644675 0.0 0.0 24 0.550326907288895 0.0 0.0 25 0.5808662795873352 0.0 0.0 26 0.611096548927289 0.0 0.0 27 0.6426868712845825 0.0 0.0 28 0.664818643112197 0.0 0.0 29 0.6881868267737564 0.0 0.0 30 0.713100525227747 0.0 0.0 31 0.742588947467334 0.0 0.0 32 0.7730046785823796 0.0 0.0 33 0.8018130743132968 0.0 0.0 34 0.8302505464940306 0.0 0.0 35 0.8585025568996726 0.0 0.0 36 0.8894746733399935 0.0 0.0 37 0.9205704309637088 0.0 0.0 38 0.9577246065737545 0.0 0.0 39 0.9940132939000386 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGATCAG 770 0.0 15.7767935 40 AGATCTC 745 0.0 15.400791 45 CAGATCT 760 0.0 15.0968275 44 CACGATC 840 0.0 13.926428 38 AGTCACG 810 0.0 13.887181 35 CACCCGT 135 2.6529745E-4 13.3333435 2 GTCACGA 855 0.0 13.15587 36 TCAGATC 905 0.0 12.926587 43 CCACCCG 140 3.54244E-4 12.885036 1 GATCAGA 970 0.0 12.755769 41 TCACGAT 935 0.0 12.030234 37 ACGATCA 1010 0.0 12.027852 39 CAGTCAC 1030 0.0 12.013085 34 CCAGTCA 1075 0.0 11.928766 33 TCCAGTC 1130 0.0 11.149071 32 ATCAGAT 1205 0.0 10.081436 42 GAACTCC 1225 0.0 9.919908 28 ACTCCAG 1255 0.0 9.682778 30 CTCCAGT 1290 0.0 9.592733 31 ACGTCTG 1335 0.0 9.439084 22 >>END_MODULE