FastQCFastQC Report
Sun 19 Apr 2015
HCNNTADXX l01n01 dna2-frb rad27-frb.34100000008942.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCNNTADXX l01n01 dna2-frb rad27-frb.34100000008942.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1617584
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAG7700.015.776793540
AGATCTC7450.015.40079145
CAGATCT7600.015.096827544
CACGATC8400.013.92642838
AGTCACG8100.013.88718135
CACCCGT1352.6529745E-413.33334352
GTCACGA8550.013.1558736
TCAGATC9050.012.92658743
CCACCCG1403.54244E-412.8850361
GATCAGA9700.012.75576941
TCACGAT9350.012.03023437
ACGATCA10100.012.02785239
CAGTCAC10300.012.01308534
CCAGTCA10750.011.92876633
TCCAGTC11300.011.14907132
ATCAGAT12050.010.08143642
GAACTCC12250.09.91990828
ACTCCAG12550.09.68277830
CTCCAGT12900.09.59273331
ACGTCTG13350.09.43908422
Produced by FastQC (version 0.11.2)