##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename HCNNTADXX l01n01 dna2-frb.341000000088c9.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3899464 Sequences flagged as poor quality 0 Sequence length 51 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.46141367121225 33.0 33.0 33.0 27.0 33.0 2 31.648938418203116 33.0 33.0 33.0 27.0 33.0 3 31.642853992240983 33.0 33.0 33.0 27.0 33.0 4 35.28988804615199 37.0 37.0 37.0 33.0 37.0 5 35.36033695913079 37.0 37.0 37.0 33.0 37.0 6 35.006391904118104 37.0 33.0 37.0 33.0 37.0 7 34.960334804988584 37.0 33.0 37.0 33.0 37.0 8 34.9295108250775 37.0 33.0 37.0 33.0 37.0 9 35.004222118732216 37.0 33.0 37.0 33.0 37.0 10 34.89578721588403 37.0 33.0 37.0 27.0 37.0 11 34.97994134578496 37.0 33.0 37.0 33.0 37.0 12 34.79302950354202 37.0 33.0 37.0 27.0 37.0 13 34.23983193587632 37.0 33.0 37.0 27.0 37.0 14 35.6432948220576 37.0 33.0 40.0 27.0 40.0 15 35.754222631623215 37.0 33.0 40.0 27.0 40.0 16 35.53896073921955 37.0 33.0 40.0 27.0 40.0 17 35.7773181134638 37.0 33.0 40.0 27.0 40.0 18 35.70727258925842 37.0 33.0 40.0 27.0 40.0 19 35.85133315758268 37.0 33.0 40.0 27.0 40.0 20 35.786434238141446 37.0 33.0 40.0 27.0 40.0 21 35.776522106627986 37.0 33.0 40.0 27.0 40.0 22 35.77491342399878 37.0 33.0 40.0 27.0 40.0 23 35.71709547773745 37.0 33.0 40.0 27.0 40.0 24 35.630071209786784 37.0 33.0 40.0 27.0 40.0 25 35.68071406736926 37.0 33.0 40.0 27.0 40.0 26 35.510583505835676 37.0 33.0 40.0 27.0 40.0 27 35.40786015719083 37.0 33.0 40.0 27.0 40.0 28 35.21464385874572 37.0 33.0 40.0 27.0 40.0 29 35.28092963545759 37.0 33.0 40.0 27.0 40.0 30 35.18095461324941 37.0 33.0 40.0 27.0 40.0 31 35.180711246468746 37.0 33.0 40.0 27.0 40.0 32 35.13491136217696 37.0 33.0 40.0 27.0 40.0 33 35.04549317547232 37.0 33.0 40.0 27.0 40.0 34 35.06231241011585 37.0 33.0 40.0 27.0 40.0 35 35.056220803679686 37.0 33.0 40.0 27.0 40.0 36 34.99661979184832 37.0 33.0 40.0 27.0 40.0 37 35.03107324493828 37.0 33.0 40.0 27.0 40.0 38 34.96496185116724 37.0 33.0 40.0 27.0 40.0 39 34.932249919476114 37.0 33.0 40.0 27.0 40.0 40 34.954156007082 37.0 33.0 40.0 27.0 40.0 41 34.780206459144125 37.0 33.0 40.0 27.0 40.0 42 34.44887810222123 37.0 33.0 40.0 27.0 40.0 43 34.60142496507212 37.0 33.0 40.0 27.0 40.0 44 34.55382765426223 37.0 33.0 40.0 27.0 40.0 45 34.57321134391803 37.0 33.0 40.0 27.0 40.0 46 34.37201600014771 37.0 33.0 40.0 27.0 40.0 47 34.47442853684506 37.0 33.0 40.0 27.0 40.0 48 34.30623644685526 37.0 33.0 40.0 27.0 40.0 49 34.32011450804521 37.0 33.0 40.0 27.0 40.0 50 34.273583241183914 37.0 33.0 40.0 27.0 40.0 51 33.16968024323343 37.0 33.0 37.0 22.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.06955845624666779 2201 2 0.04923445855351716 2201 3 -0.43115572840049055 2201 4 -0.21311442103660738 2201 5 -0.03804832832121008 2201 6 -0.21530103566818326 2201 7 0.03466854749229498 2201 8 0.03820965904676399 2201 9 -0.13478448208846316 2201 10 0.10580464325958872 2201 11 0.06533677066038734 2201 12 0.06589476927097593 2201 13 0.7534136883172593 2201 14 -0.02951355967999092 2201 15 -0.04659979583844631 2201 16 0.3025589255328214 2201 17 0.05580943379094094 2201 18 0.08051373459108646 2201 19 0.00953825170948619 2201 20 0.04758586952646482 2201 21 0.02112581118360879 2201 22 -0.11140901374928802 2201 23 -0.3758614614251101 2201 24 -0.09187020200100449 2201 25 -0.1437717738581128 2201 26 -0.22961594269560237 2201 27 -0.24068135652719747 2201 28 -0.0951991752308814 2201 29 -0.11554452490721445 2201 30 -0.2765238802282681 2201 31 -0.3534653332525437 2201 32 -0.24742976132654348 2201 33 -0.44843343688422976 2201 34 -0.25536577616411904 2201 35 -0.36837015597629374 2201 36 -0.14918833142315435 2201 37 -0.1975728762969382 2201 38 -0.1604625606521708 2201 39 -0.1043554333925627 2201 40 -0.11609998229632623 2201 41 -0.026152335130511517 2201 42 0.22962746085281083 2201 43 0.050635838451128734 2201 44 -0.14615500857554764 2201 45 -0.22473981541124033 2201 46 -0.2959963430695538 2201 47 -0.15947878297546225 2201 48 -0.17771908906554756 2201 49 -0.17417099297041005 2201 50 -0.21134109990569527 2201 51 -0.36338293239010966 1101 1 0.08854135727572654 1101 2 0.042246240246345934 1101 3 0.12348569024375422 1101 4 -0.5722680542816221 1101 5 -0.18193621970859652 1101 6 -0.2527520234775764 1101 7 -0.19331519300040867 1101 8 -0.1652435598193236 1101 9 -0.14045137568635369 1101 10 -0.17615009252727987 1101 11 -0.0960602518631859 1101 12 -0.18714460786512888 1101 13 0.2639506242769727 1101 14 -0.14917483301599788 1101 15 -0.12587940464258907 1101 16 0.06453801153150351 1101 17 -0.05066069607639179 1101 18 -0.3038614606255976 1101 19 -0.2223414129146093 1101 20 -0.13007289152487544 1101 21 -0.321522791656335 1101 22 -0.19971244319032877 1101 23 -0.11749721329169915 1101 24 -0.1334434268142317 1101 25 -0.061218512433207195 1101 26 -0.09849994733369982 1101 27 -0.1527774850672614 1101 28 -0.11356041795161076 1101 29 -0.10104807470698773 1101 30 -0.38594844969545505 1101 31 -0.27564345317522054 1101 32 -0.14860933015349787 1101 33 -0.03155763602720896 1101 34 -0.007105331895139955 1101 35 -0.2480284986175434 1101 36 -0.727097348062621 1101 37 -0.3133466016388553 1101 38 -0.35569922167413637 1101 39 -0.16929602266935717 1101 40 -0.2523566200115539 1101 41 -0.14429277143239716 1101 42 0.0739365124623319 1101 43 -0.035397912852744184 1101 44 -0.28711052533995485 1101 45 -0.18607457625891755 1101 46 -0.16144460613770661 1101 47 -0.1391293425851714 1101 48 -0.42808374990987375 1101 49 -0.2913110464021784 1101 50 -0.3984684284622091 1101 51 -0.3449528667288746 1102 1 0.012058329018202585 1102 2 -0.0701602569616675 1102 3 0.06834637474504746 1102 4 0.06041526480883874 1102 5 -0.1419617798776187 1102 6 -0.11716412368400597 1102 7 -0.34583969872404197 1102 8 -0.12418707085257097 1102 9 0.010243419845302526 1102 10 -0.20870676112975417 1102 11 -0.12500636550647926 1102 12 -0.5738858473286683 1102 13 0.5856664624659942 1102 14 0.16180447896973504 1102 15 0.0935876051620852 1102 16 -0.7732792862484885 1102 17 -0.48569760856432964 1102 18 -0.5710099172262488 1102 19 -0.3551113286872223 1102 20 -0.21782581843167748 1102 21 -0.18642560766436134 1102 22 -0.3179871531328331 1102 23 -0.12085239962788563 1102 24 -0.48136646358993573 1102 25 -0.2283395854053083 1102 26 -0.19472137015883817 1102 27 -0.0799526940852644 1102 28 -0.2962643961209537 1102 29 -0.14927546257187174 1102 30 -0.2596085010113356 1102 31 -0.21383399069075892 1102 32 -0.18615301532152273 1102 33 -0.36597101279748045 1102 34 -0.2964704524779904 1102 35 -0.2601179550098678 1102 36 -0.15891910499859563 1102 37 -0.1400348084447458 1102 38 -0.19339995915461827 1102 39 -0.21587020057078377 1102 40 -0.20012262529343872 1102 41 -0.12220926554231681 1102 42 0.1560362692860764 1102 43 -0.12352478707516923 1102 44 -0.0626017979768676 1102 45 -0.3718677889065063 1102 46 -0.3208624118845407 1102 47 -0.2317657983445045 1102 48 -0.1913787652300698 1102 49 -0.3107988872934513 1102 50 -0.2988627138179609 1102 51 -0.689560306870753 1103 1 -0.21850335537597942 1103 2 -0.0462000664727924 1103 3 0.018074136579997457 1103 4 0.17388380439526685 1103 5 0.05298196689490453 1103 6 0.12728289951498795 1103 7 0.09831081214549187 1103 8 0.08762865815908327 1103 9 0.025202996784777554 1103 10 0.17706914265591678 1103 11 0.05572776030327731 1103 12 0.12781162460878903 1103 13 0.7675008251877173 1103 14 0.43703293424464107 1103 15 0.29644135446445574 1103 16 0.5055958448981599 1103 17 0.16819803523465282 1103 18 0.18472660013632236 1103 19 0.06705547360655117 1103 20 0.08447541998886265 1103 21 0.009413099116819978 1103 22 0.16136870077780685 1103 23 0.03945041131236593 1103 24 0.08783251541553483 1103 25 0.036408883553868066 1103 26 0.08598860078998882 1103 27 0.15880804898618095 1103 28 0.2384768992137225 1103 29 0.13424020488857025 1103 30 0.17215475490340282 1103 31 9.502525726503563E-4 1103 32 -0.15978157174567542 1103 33 -0.04013489043813934 1103 34 0.002566570962343917 1103 35 0.08842496557009838 1103 36 0.13041292947021077 1103 37 -0.13389112499561406 1103 38 0.14852830415534868 1103 39 -0.18428762993904257 1103 40 -0.023980701120152048 1103 41 0.04847589278540454 1103 42 0.26123260761716693 1103 43 0.16344451771928448 1103 44 0.07311644335059952 1103 45 0.1160157781416089 1103 46 -0.07371840919692119 1103 47 -0.05267771426139234 1103 48 0.04152103045777267 1103 49 0.00835376985149594 1103 50 -0.010078017981442144 1103 51 0.08506640829463663 2103 1 -0.03312343019597819 2103 2 -0.04684934978568478 2103 3 0.06363553755301155 2103 4 0.1630339774065206 2103 5 0.015638851052919733 2103 6 0.10899777597416715 2103 7 0.13709448772110733 2103 8 0.07246626752543506 2103 9 0.03707099285580995 2103 10 0.1522579306770666 2103 11 -0.00556903918710816 2103 12 0.20234361599563044 2103 13 0.8438560630461325 2103 14 0.4695335498847726 2103 15 0.2593067457877254 2103 16 -0.2550833726562942 2103 17 0.07340458301393937 2103 18 0.16934396530438534 2103 19 0.1410843813801108 2103 20 0.2396029537361528 2103 21 0.1501100902246506 2103 22 0.07823738416114878 2103 23 0.17301948093275143 2103 24 0.17123245165372225 2103 25 0.14585177557054863 2103 26 0.1341508100502864 2103 27 -0.01448324270293 2103 28 0.3535056055225638 2103 29 0.2831174563350771 2103 30 0.2772154757983927 2103 31 0.26103454998149545 2103 32 0.04245719741302878 2103 33 0.11308694565171606 2103 34 0.14317719319001299 2103 35 0.218143398351053 2103 36 0.2971609643393478 2103 37 0.17251687789971015 2103 38 0.17219356915185102 2103 39 0.30270656122927875 2103 40 0.15011789162807787 2103 41 -0.0033205927999162554 2103 42 0.5447491291183155 2103 43 0.3644429862103422 2103 44 0.2175601102430349 2103 45 0.2839749785975485 2103 46 0.1367966695387821 2103 47 0.08806219531741277 2103 48 0.2869250012622828 2103 49 0.2502684805445128 2103 50 0.3224061872122448 2103 51 0.18198994667410773 2102 1 -0.19279268426842222 2102 2 -0.0830890105318538 2102 3 -0.14903394140495152 2102 4 0.03298256325242477 2102 5 0.0045593644223771435 2102 6 0.004848530291923225 2102 7 -0.02189956741465693 2102 8 0.0744809690887962 2102 9 0.011660507852475632 2102 10 -0.20087847908691714 2102 11 -0.11647454527293633 2102 12 -0.03619612565655217 2102 13 0.549426980474891 2102 14 0.17722783139782905 2102 15 0.09080520657017388 2102 16 -0.26035442907529216 2102 17 -0.09786697791414412 2102 18 -0.04801972533577015 2102 19 -0.044670496454891406 2102 20 0.08825924681501363 2102 21 0.14795568472989373 2102 22 0.16763853523063688 2102 23 0.14939801169136047 2102 24 -0.01921385954039323 2102 25 0.07817843250053613 2102 26 0.010144114083793454 2102 27 0.00875657091587101 2102 28 -0.6136205959368723 2102 29 -0.3349675929499867 2102 30 -0.13115166382623755 2102 31 -0.159465169350824 2102 32 0.051572151726738014 2102 33 0.08498576455539819 2102 34 -0.11577725298408126 2102 35 -0.05142796593521837 2102 36 -0.0270272774947955 2102 37 0.03701391010984878 2102 38 0.13244746148947684 2102 39 0.09757238524642275 2102 40 0.012592682117045229 2102 41 -0.19074864556441895 2102 42 0.1675801833420465 2102 43 0.11657604824429768 2102 44 0.18504335786928294 2102 45 0.0998973083635164 2102 46 0.16933870660632522 2102 47 0.11187131507826109 2102 48 -0.014511859275600614 2102 49 0.09606865672161291 2102 50 0.03791177601973317 2102 51 -0.01097808386330712 2101 1 0.15305911639650915 2101 2 0.09444965359431734 2101 3 0.19029031754705272 2101 4 0.11437069751234219 2101 5 0.19500258323079578 2101 6 0.20668657222712028 2101 7 0.10546003236757429 2101 8 0.15388861445911317 2101 9 0.21341914042400134 2101 10 0.036404278763193076 2101 11 0.23572437558623704 2101 12 0.2619360421663828 2101 13 -4.583277841896891 2101 14 -1.5717316511691308 2101 15 -0.812096484376923 2101 16 -0.03077624030517967 2101 17 0.009863141225068262 2101 18 0.18440364572312973 2101 19 0.17858733850210484 2101 20 -0.10507000594569149 2101 21 0.06474109339713152 2101 22 0.01292955136261753 2101 23 0.013383532462505343 2101 24 0.23184785098560923 2101 25 0.12726508978730067 2101 26 -0.0020447892113395483 2101 27 0.1821453211431745 2101 28 0.26283166879613873 2101 29 0.02026869870579162 2101 30 0.1784973372976708 2101 31 0.3641865179068162 2101 32 0.2862362496491926 2101 33 0.284404587122971 2101 34 0.20667799780446927 2101 35 0.267441253206826 2101 36 0.34858415627114425 2101 37 0.2570023311909537 2101 38 0.20860252667762325 2101 39 9.933247841473758E-4 2101 40 0.1945764788843789 2101 41 0.11521306802437437 2101 42 -2.157191896108806 2101 43 -0.867377465864557 2101 44 -0.37327657043449136 2101 45 -0.18703959593548802 2101 46 0.0646532581068513 2101 47 0.08820683940956542 2101 48 0.07618266999238443 2101 49 0.032999568302109594 2101 50 0.32327065773583286 2101 51 0.3783349642987801 2104 1 0.12120221090327021 2104 2 0.060368331357825156 2104 3 0.11635761313657866 2104 4 0.24069616794283633 2104 5 0.09376356230642813 2104 6 0.13740140482154573 2104 7 0.1855205794126391 2104 8 -0.13724353760731844 2104 9 -0.02236119998750752 2104 10 0.1141993373881931 2104 11 -0.013678704720184953 2104 12 0.1392405288085925 2104 13 0.819463198127913 2104 14 0.5048212493681916 2104 15 0.2444347728734826 2104 16 0.44680054632275557 2104 17 0.32695008929026415 2104 18 0.3039031574326998 2104 19 0.22585779285844154 2104 20 -0.006954774164242394 2104 21 0.11460262066862015 2104 22 0.208934438540183 2104 23 0.23895963794569752 2104 24 0.23498113389067043 2104 25 0.04562569028435348 2104 26 0.29459852447539703 2104 27 0.13818483733745524 2104 28 0.2638304117078789 2104 29 0.2632092952065861 2104 30 0.42536492676183 2104 31 0.37623662600840646 2104 32 0.36170807975830854 2104 33 0.4036196788169448 2104 34 0.3222970515644761 2104 35 0.3539349584109672 2104 36 0.28607401189850634 2104 37 0.31831229217568335 2104 38 0.0477898800066896 2104 39 0.27253701531193286 2104 40 0.23527287609199732 2104 41 0.3230346496598244 2104 42 0.7240297334300436 2104 43 0.3312007751674386 2104 44 0.3934239908639512 2104 45 0.46983371140946417 2104 46 0.48123313603673523 2104 47 0.2949112883612912 2104 48 0.40706476176869444 2104 49 0.38859045124632985 2104 50 0.23516163919952504 2104 51 0.7634828705854986 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 5.0 10 11.0 11 20.0 12 16.0 13 12.0 14 22.0 15 32.0 16 52.0 17 139.0 18 361.0 19 834.0 20 1784.0 21 3590.0 22 6725.0 23 11558.0 24 19210.0 25 30040.0 26 44204.0 27 63968.0 28 88527.0 29 118200.0 30 154433.0 31 197869.0 32 244768.0 33 299605.0 34 362548.0 35 434483.0 36 513968.0 37 605353.0 38 682034.0 39 15092.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 22.34221458354207 26.106148538074798 16.73297417834948 34.818662700033656 2 17.93218247430929 30.508090342672737 24.40302051769166 27.156706665326315 3 16.669649982664282 28.515047196230046 28.924795818091926 25.890507003013745 4 16.5959475456114 27.809078375899865 30.636005358685193 24.958968719803543 5 17.248883436287656 28.452807873082044 30.648263453643885 23.650045236986415 6 18.29937763342719 28.743348869201974 31.344762664878928 21.61251083249191 7 17.907358549790434 31.66209509819811 29.299052382583863 21.13149396942759 8 18.314670939390645 29.721828435908115 30.098752033612826 21.864748591088418 9 18.831844581716872 29.374626871795712 29.970195903847298 21.823332642640118 10 18.998226422913508 29.158033001458662 29.889979751063223 21.953760824564608 11 18.712751290946652 29.271279334800887 30.19497038567352 21.820998988578943 12 20.736721764837423 29.184498177185375 29.0985376451738 20.980242412803403 13 19.154837690513364 29.212809760520937 30.02451106100736 21.607841487958346 14 18.732395016340707 29.879337262762267 29.93098538670956 21.457282334187468 15 19.38161244724916 29.937242656939517 29.80335246074845 20.87779243506287 16 19.896580658264828 29.016654596631742 29.85974482646846 21.22701991863497 17 18.953366379468413 29.58831839897297 30.475660723952956 20.982654497605655 18 20.085960950853263 29.261420535486458 30.111746671641733 20.540871842018543 19 19.120394803990507 29.05981196341847 30.09898308840161 21.720810144189418 20 19.24587584344925 29.598785884418987 30.232026760600945 20.923311511530816 21 19.73072079194819 29.149492222871604 29.957478586066177 21.16230839911403 22 19.487454611618553 29.43646985780636 29.859816930856812 21.216258599718266 23 19.2866439028018 29.04826443695337 29.87781625893875 21.78727540130608 24 20.340155767953448 29.205157729798763 29.772502878655377 20.682183623592408 25 19.15142696534703 28.991625515711906 30.59766675625163 21.259280762689436 26 18.954321965553966 29.180372778891595 30.38432178086774 21.4809834746867 27 19.504965527004444 29.12391095390846 30.69160902360536 20.679514495481737 28 19.984478413658994 28.759124574323312 30.270607008924657 20.985790003093033 29 19.341145347155404 29.356393596658414 30.459878588441896 20.842582467744286 30 20.015057478782936 29.350463459962366 29.995985527377293 20.6384935338774 31 19.571680516307854 28.504923476276605 30.168778156727004 21.754617850688533 32 19.49577633826222 28.782010842526912 30.721510157816724 21.00070266139414 33 19.727535418625962 29.08534657177759 29.989723398618516 21.197394610977934 34 19.31288505292009 28.874891523552982 30.006534231371283 21.80568919215564 35 19.27577814761895 29.36305502144027 30.298506965042826 21.062659865897952 36 20.14243496029198 28.938695164258473 30.218050480784026 20.700819394665523 37 19.172839968425247 28.665730776510152 30.868090266041392 21.29333898902321 38 19.05122040353138 28.952754643431877 30.302578010296656 21.69344694274009 39 19.56727899013813 29.714596672773492 30.125704455791873 20.592419881296507 40 19.55622618903521 28.683224155935278 30.148835840002626 21.611713815026885 41 19.916173094558637 28.877379044914893 30.246438997769946 20.96000886275652 42 19.995978934540748 28.91289674683495 29.86251443788172 21.228609880742585 43 19.209075914023053 28.721639692019213 30.100059905669085 21.969224488288646 44 19.143387040686218 29.413674500000514 30.285756489966158 21.15718196934711 45 19.631492943645586 29.312669638699063 30.231693381449347 20.824144036206 46 19.287387339098448 28.514816240557998 30.79889956942461 21.398896850918945 47 19.083005144594097 29.48131177019839 30.28624930855332 21.149433776654195 48 19.4244779591887 29.112802463614095 30.761631795557783 20.701087781639426 49 19.1555233076316 28.789915462050253 30.2120130014191 21.842548228899048 50 19.174217558519466 28.892153184479064 30.892471692650386 21.04115756435109 51 19.559978385659033 28.999941783870437 30.10084213350061 21.339237696969914 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1.0 1 1.0 2 1.0 3 3.5 4 6.0 5 11.0 6 16.0 7 35.0 8 54.0 9 109.0 10 164.0 11 318.0 12 472.0 13 814.5 14 1157.0 15 1941.0 16 2725.0 17 3900.5 18 5076.0 19 7401.5 20 9727.0 21 13553.0 22 17379.0 23 24154.0 24 30929.0 25 41983.0 26 69652.0 27 86267.0 28 106943.0 29 127619.0 30 154885.0 31 182151.0 32 214300.5 33 246450.0 34 276841.0 35 307232.0 36 333372.5 37 359513.0 38 375034.5 39 390556.0 40 389723.0 41 388890.0 42 375928.0 43 362966.0 44 342825.0 45 322684.0 46 296294.5 47 269905.0 48 243313.5 49 216722.0 50 194118.5 51 171515.0 52 149667.5 53 127820.0 54 105986.0 55 84152.0 56 67142.0 57 50132.0 58 39495.5 59 28859.0 60 23645.5 61 18432.0 62 15860.5 63 13289.0 64 10573.0 65 7857.0 66 6554.0 67 5251.0 68 4035.5 69 2820.0 70 2447.0 71 2074.0 72 1871.5 73 1669.0 74 1479.5 75 1164.0 76 1038.0 77 876.5 78 715.0 79 580.0 80 445.0 81 337.5 82 230.0 83 169.5 84 109.0 85 77.0 86 45.0 87 30.5 88 16.0 89 10.5 90 5.0 91 2.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17935798355876603 2 0.0 3 0.0 4 0.0 5 0.0 6 0.008078033288677624 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.001718184858226669 18 4.87246452332936E-4 19 2.564455012278611E-4 20 0.0 21 4.3595735208736377E-4 22 0.0016156066577355248 23 2.3080095110507496E-4 24 0.001897696709086172 25 0.0 26 0.024618768117874664 27 0.004616019022101499 28 0.009693639946413148 29 0.0 30 0.02741402408125835 31 0.002179786760436819 32 1.0257820049114442E-4 33 0.008078033288677624 34 0.0 35 0.01264276321053355 36 0.0 37 0.0037441043179267715 38 0.0035645924670672686 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0015386730073671665 45 0.0 46 0.0067445166822927465 47 7.949810538063692E-4 48 0.003897971618663488 49 0.012950497812006984 50 8.975592542975136E-4 51 0.005154554574680007 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 3899464.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.50591666759691 #Duplication Level Percentage of deduplicated Percentage of total 1 68.19860023160273 40.58220222228506 2 19.675385849354218 23.416037415089754 3 7.010823363632826 12.515564125427293 4 2.634392271978258 6.270477080243982 5 0.9795005533847416 2.9143039152788743 6 0.42251461060176687 1.5085271525586534 7 0.25167349571857456 1.0483243444570614 8 0.12334245124385179 0.5871684500235023 9 0.08172944556226205 0.4377047019225177 >10 0.5430495041443226 6.407197556126833 >50 0.05848883161991554 2.413480620301137 >100 0.020214676146946893 1.758420904989851 >500 1.9341819445182205E-4 0.06147437925077305 >1k 9.129681508740103E-5 0.07911713204471765 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.06149563119444108 0.0 0.0 2 0.06231625679837024 0.0 0.0 3 0.06359848430450954 0.0 0.0 4 0.07754911957130518 0.0 0.0 5 0.08098548928775852 0.0 0.0 6 0.10850209156950802 0.0 0.0 7 0.6901974220046653 0.0 0.0 8 0.7016605359095507 0.0 0.0 9 0.716252284929416 0.0 0.0 10 0.7824921578965724 0.0 0.0 11 0.9651326438710551 0.0 0.0 12 1.0549655029511749 0.0 0.0 13 1.138387224500598 0.0 0.0 14 1.176751471484286 0.0 0.0 15 1.2167313251257097 0.0 0.0 16 1.255403306710871 0.0 0.0 17 1.2939983546456642 0.0 0.0 18 1.335363013993718 0.0 0.0 19 1.3985768300463859 0.0 0.0 20 1.4275551716851342 0.0 0.0 21 1.462713849903474 0.0 0.0 22 1.4920512152439411 0.0 0.0 23 1.5294409693229634 0.0 0.0 24 1.5624967944312347 0.0 0.0 25 1.6266851033885683 0.0 0.0 26 1.6786666064874558 0.0 0.0 27 1.7222366971460692 0.0 0.0 28 1.750932948733467 0.0 0.0 29 1.7828091245360902 0.0 0.0 30 1.8143775657372399 0.0 0.0 31 1.8475872581462478 0.0 0.0 32 1.8810533960564837 0.0 0.0 33 1.912288458106037 0.0 0.0 34 1.9440620557081691 0.0 0.0 35 1.973681511099987 0.0 0.0 36 2.005711554203347 0.0 0.0 37 2.038587867460759 0.0 2.5644550122786104E-5 38 2.0726694745739414 0.0 2.5644550122786104E-5 39 2.109879716802104 0.0 2.5644550122786104E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATATCTC 4220 0.0 23.352821 45 GCCAATA 5205 0.0 20.400091 41 TCACGCC 5370 0.0 20.108929 37 CCAATAT 5060 0.0 20.006836 42 CACGCCA 5370 0.0 19.89895 38 CGCCAAT 5385 0.0 19.843521 40 GTCACGC 5455 0.0 19.795591 36 AATATCT 5165 0.0 19.472195 44 ACGCCAA 5535 0.0 19.346151 39 CAATATC 5215 0.0 19.2855 43 AGTCACG 5835 0.0 18.931738 35 CAGTCAC 6625 0.0 16.844015 34 CCAGTCA 7045 0.0 15.871969 33 TCCAGTC 7145 0.0 15.61834 32 ACTCCAG 7470 0.0 15.124193 30 GAACTCC 7415 0.0 15.11497 28 CTCCAGT 7475 0.0 14.928834 31 TGAACTC 8080 0.0 13.815271 27 CTGAACT 8215 0.0 13.755614 26 ACGTCTG 8215 0.0 13.724161 22 >>END_MODULE