FastQCFastQC Report
Sun 19 Apr 2015
HCNNTADXX l01n01 dna2-frb.341000000088c9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCNNTADXX l01n01 dna2-frb.341000000088c9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3899464
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCTC42200.023.35282145
GCCAATA52050.020.40009141
TCACGCC53700.020.10892937
CCAATAT50600.020.00683642
CACGCCA53700.019.8989538
CGCCAAT53850.019.84352140
GTCACGC54550.019.79559136
AATATCT51650.019.47219544
ACGCCAA55350.019.34615139
CAATATC52150.019.285543
AGTCACG58350.018.93173835
CAGTCAC66250.016.84401534
CCAGTCA70450.015.87196933
TCCAGTC71450.015.6183432
ACTCCAG74700.015.12419330
GAACTCC74150.015.1149728
CTCCAGT74750.014.92883431
TGAACTC80800.013.81527127
CTGAACT82150.013.75561426
ACGTCTG82150.013.72416122
Produced by FastQC (version 0.11.2)