FastQCFastQC Report
Wed 21 Jun 2023
HCMTGBGXT_n01_pIXHALO_pegF1CV-B_ab3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMTGBGXT_n01_pIXHALO_pegF1CV-B_ab3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences399780
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTA67411.686177397568663TruSeq Adapter, Index 19 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT31160.7794286857771775TruSeq Adapter, Index 19 (97% over 38bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGT6580.16459052478863376TruSeq Adapter, Index 19 (97% over 38bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGT6470.16183901145630097TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)