FastQCFastQC Report
Wed 21 Jun 2023
HCMTGBGXT_n01_pIXHALO_Col-B_ab2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMTGBGXT_n01_pIXHALO_Col-B_ab2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176365
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC4660.26422476114875404TruSeq Adapter, Index 27 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG2220.1258753153970459TruSeq Adapter, Index 27 (97% over 38bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)