FastQCFastQC Report
Wed 21 Jun 2023
HCMTGBGXT_n01_daplib_pegCvi-input_ab3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMTGBGXT_n01_daplib_pegCvi-input_ab3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3890470
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT1555963.999413952555861TruSeq Adapter, Index 2 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCC405421.0420848894863604TruSeq Adapter, Index 2 (97% over 34bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCCG60930.15661346829560438TruSeq Adapter, Index 2 (96% over 33bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG51590.13260608615411504TruSeq Adapter, Index 2 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)