FastQCFastQC Report
Wed 21 Jun 2023
HCMTGBGXT_n01_daplib_pegCvi-input_ab1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMTGBGXT_n01_daplib_pegCvi-input_ab1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16339937
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT4789512.9311679720674566TruSeq Adapter, Index 2 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCC1171420.7169060688544883TruSeq Adapter, Index 2 (97% over 34bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG235770.14429064200186328TruSeq Adapter, Index 2 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)