FastQCFastQC Report
Wed 21 Jun 2023
HCMTGBGXT_n01_DEL2_An1AmpPoolHm-B_ab.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMTGBGXT_n01_DEL2_An1AmpPoolHm-B_ab.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTTAAGTTCTTATACTCAATCATACAAATGACATCAAGTCATATTCGA54.901960784313726No Hit
CATGCTGTCATCTTTATTTTGGTAGCCGGAATTGTGATAGAAGAGAAAAA32.941176470588235No Hit
CAATAAAGAAAAAACAAACGAAAGCTAAGGCCTACGCGGGAACTCCGTGA21.9607843137254901No Hit
ATCCACCGTCAATCGGCCAAATAGCGATAAAAAACCTCCGGCCTTCTGGT21.9607843137254901No Hit
GAAACAATTCTAAGTCATAAAATTAAGCTGTAGCGTTATGTATTAATTTG21.9607843137254901No Hit
ACCCGTGATTTTCCTGCCAAAAACGTAAACCCGTAATTTTCCCTCCAAAA21.9607843137254901No Hit
CTTCTTATTGCTTCTCAAAGCTTTCATGGTGTAGCCAAAGTCCATATGAG21.9607843137254901No Hit
ATCAAGCAGCTCGTCAGCTCACCCGTCCAAAGTACCTCGTCTGCCCATCC21.9607843137254901No Hit
CAATAAAAATAACAAGAGCATTTTTATTGAGATCGGAAGAGCACACGTCT21.9607843137254901Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TATAAGTTTAGTTTATGGTACATCCAATCTCGATACAAACCGCCACCCCC10.9803921568627451No Hit
ATATCTATGAAGTTAGGTTTTATGTGTCAGAATTCGGATTGAGCATGAAT10.9803921568627451No Hit
CGGCTTCTCCGGTCATTCTTAGCTATAGACATAGGGAGTTTCGCGGAACG10.9803921568627451No Hit
ACGTAATCACGGATTTACGTTTTAGGCGGGAAAATCTCGGGTTTACGATT10.9803921568627451No Hit
AAAGAAAAATTACAGGTTTACGTTTTTGGCGGGAAAATTACGAGTTTACG10.9803921568627451No Hit
AGTGGTATTTCACTTGCGCCGAAGCTCCACTTATCCTACACCTCTCAAGT10.9803921568627451No Hit
ACAAATGGGTGAAAATGAGGAAGCTTTGTGGTTGTTTAAAGAAGCTTTGT10.9803921568627451No Hit
GAATCCTTTCCCGCTAAATTACACTTCAGTCACCGATAATAATCTCCCGC10.9803921568627451No Hit
ACTGCACAGTCTGGCAACAGACTGTGCAGTCGAGTAAGATTGTCTTGTTT10.9803921568627451No Hit
AACGAAAAGCATATATCTCGTGTCTGCCATTTGACGTTCATGTCGACGTA10.9803921568627451No Hit
TTTATTTTTCCCGCTTTCTCTGGATTTGAATGCCACTGGGGACAATGTCG10.9803921568627451No Hit
ACGTCGTTTTGTCTTTGTTTTGTAATGTGAATCTATCTATATATGTTATG10.9803921568627451No Hit
AACCACAAGAGAGCACGAGGCGCTCTGTGCATAGGTGGTGTTGTGACACC10.9803921568627451No Hit
AATCACGGGTTTACGTTTTTTTGGGGAGAAATTACGGGTTTACGTTTTTG10.9803921568627451No Hit
GAAATAAAAAGGAAAACTAATATTTGTGTCAATTATTTTAACTATAGAAA10.9803921568627451No Hit
TTGAGTTGTAACCATAAGAGTTGTGTGAGAATATTGAGAGACCTTGGTGC10.9803921568627451No Hit
CACACAAAAAAAAACTTGGTTACTAATTAAGAAAATGAAAACATATAAAC10.9803921568627451No Hit
AGGTTTGTCTCCATAAATCCTCGATAGAAAATTAATTCCATTTGATTGAG10.9803921568627451No Hit
TCTGAATTTCGTGAACTCTTACTGAATTGCGTAGAATCTTTCCTTAATAT10.9803921568627451No Hit
CTTGTATAACGTTTTTCTGTGTGTATAGTGATTGCGGGAATGGAGAGATA10.9803921568627451No Hit
ATTTCTGTCATTCTTTGGACATGTTCAAGGTGTTTTGTTAACTTCACTCA10.9803921568627451No Hit
AGTTAACTTAAAAACATTTTCCCGCCAAAAATTTTCAAAAAAAAATTTCC10.9803921568627451No Hit
CGCCGCTGACGCCCATGTCTTCCATGCAAGAGACCAAGAGACATGGAATC10.9803921568627451No Hit
AAGTCATATTCGACTCCAAAACACTAACAAACCTTCTTCTTGCTTCTCAA10.9803921568627451No Hit
CTTTTTTTATTTAAAGTTGAAATTTTAATGTCAGTAAATATATAAAAATT10.9803921568627451No Hit
ATTTTGGTGGAAAATCACAAAAATTGTTTACCCGCCAAAATCACAAAAAT10.9803921568627451No Hit
GTTTTCGCTCGGATCTAGATTGTTTGTGTTTACGATATTCTTTATGGGTT10.9803921568627451No Hit
AAAGAAAAAAGTGAAATTTTTGGTTTTCCCCCTAAATTTTTGGCGCCTAA10.9803921568627451No Hit
GCTGATAAAGATGGACAGTAACGATTGCGTAATATAAATTTATCGGCCTC10.9803921568627451No Hit
ATCTTTTTAGGGTTTATGTTCGTTGTCAATTGAAATTTCCGGTGTATCTG10.9803921568627451No Hit
ATGCGCGAGCATGGAGCCTACGAACACTAGCTATCCGATCACCACTCATA10.9803921568627451No Hit
ATAACGATGTTCTTTTTTTGTTTGTCGGTATCGTTGTTAATCTTGTTTCA10.9803921568627451No Hit
TATTTTCCCGCCAAAATCGTAAAATCGTATTTTCCCGCCAAAATCGTAAA10.9803921568627451No Hit
AAGAATCCGGTTAAAACTTCCCGTGTAGCAATCCGCGGGATCGCTCATTT10.9803921568627451No Hit
CTGAATAATTAGTCTAAGCCATTTGTTCAGTATAACCAAGGTTATGTCTT10.9803921568627451No Hit
ATATCTTTGGTTTGTTCTGATCAAATTTGCTTAGAGGGAATGTAATTCAT10.9803921568627451No Hit
CGGTCTTACATGTAAAGGGTCTTGTACCCATGTCTCAAAATTTCCTTGCC10.9803921568627451No Hit
TGTTTAAGTTCTTATACTCAATCATACAAATGACATCAAGTCATATTCGA10.9803921568627451No Hit
GTTGACTTCGATAGTAGGTTTCGATCTTTTTGCAATGACAATATTGTTAT10.9803921568627451No Hit
ATTATCCGTGAACGTATTGAGCAATATAATAGAGAAGTAACGATTGTAAA10.9803921568627451No Hit
AAACTATCACAGCCCAGAACTCATAAAGATCTACCATCTTAAGTTATTTT10.9803921568627451No Hit
AAAGTGGGATCAGTGTACAAGAAATTGAATTTCCGCTAGGGTTGGCGGAA10.9803921568627451No Hit
AACGGAAAAATCCATTAGATAATGAACTATTGGTTCTAAGCCATCTCTGG10.9803921568627451No Hit
AAAGCCTTTAAAAATCTTTTAAAACCCATTGTAAATCTTTTTCCCGCCAA10.9803921568627451No Hit
ATCGAAATGAATGAATCTATAGATATATAAAATAAATTAATAGAGCCACC10.9803921568627451No Hit
GGATCAACCAGGTAGCATTCATCGATCACGGCAATTCCCCGCCACATCCT10.9803921568627451No Hit
ATTTAGTAATCACACGTGGCTCAAACAACTTCGTCATTTTTCCAAGTTAG10.9803921568627451No Hit
CTCGCATTGGTGAATGAGTTGCGGGTTGAAATCTCGGATGCGGAAAAGTT10.9803921568627451No Hit
CTAGCAAGTAATTCCTGAATCCGTCACTCTTTGATTACTCTAGTAGCCAA10.9803921568627451No Hit
GAGTTTGTTTTCCCTGACGAATAAAGCGATGAAGAACCCTTCTTTGTCCT10.9803921568627451No Hit
CTAGGATGAGATTTGGTCATCCTAGCCTCACCGACCGGTTCGTCATTATA10.9803921568627451No Hit
AACCCTAAATGCTTTTTATGGAAAAAATATTTAGGGTTAGATCGGAAGAG10.9803921568627451No Hit
TCTCTAGGGAATGAAATCACAAACCTCCTTCTTTATCCATACACATTTTC10.9803921568627451No Hit
ACTCAGTCACAGCATAAAACAAAATCGAGACCGAATAAAGGAAAACAAAG10.9803921568627451No Hit
ATTTCTGTCAATCTTTGGACATGTTCAAGGTGTTTTGTTAACTTCACTCA10.9803921568627451No Hit
GTGTATGCCTAGGCAAACAAAGAGCACACAACAGAAGGAGCTTATCAACT10.9803921568627451No Hit
ATTTAGTTTTATTATTAGCAAATGTCAAGAACTCCTCCACTCCTGCGACG10.9803921568627451No Hit
AGTTTAGATGGATGAATTGTAACACTGCGGAAGGAGACTGGAGCAGATTA10.9803921568627451No Hit
CGTCAAAAAGGAGTTGACATTTTTCTGTCAAAAACGCAATTTAACATTTT10.9803921568627451No Hit
ATTCATCTAATCTACTTTCTTCCAGGATTTGTAATCATTCGGAGGTGACC10.9803921568627451No Hit
ATTTCATATAGACTTGTCCATGGCAACCTTGCTCTTACTTAGAATGGATA10.9803921568627451No Hit
ATCGAACTCGTGTGGAACCTCCGTTTCCCGACCAAGACCAATGGTTCCTG10.9803921568627451No Hit
TTTATTTTTGAGCGATAGAAAAGATGTTCTTTTTTTCTTTTTGGTTCATT10.9803921568627451No Hit
GAGGGTCGTGGGACACGGAAATAGGTTGTGGAACCCCTGGTCGAATCTGT10.9803921568627451No Hit
ACTTAAACCGCAACTGGATCTTAAAGGCGTAAGAATTGTATCCTTGTTAG10.9803921568627451No Hit
GTTAACAATATATTTTGTTTTCATTTTTGTGGTATTTTTAATTTCAAATT10.9803921568627451No Hit
CCCTAAAAAATCTATTTCCCGCCAAAAAAATTTTTTTTGCGGAAAAAATA10.9803921568627451No Hit
ATTCGATTTCTCTAACCGAAACTAAATCATCACCAAAGAAGGTACACCGT10.9803921568627451No Hit
CGAATAAATTTTATTGATGAAAATCAAGTGAATACAAGGTAGAGTATGAG10.9803921568627451No Hit
ATCCTAAGAGTCGGGGGAAACCCGTCTGATAGCGCTTAAGCGCGAACTTC10.9803921568627451No Hit
TATTAATAAAAACTGAAAGAACTAGATAATCGTATCAGCATGTTCCAATT10.9803921568627451No Hit
CTCGGAAGACATGACGTTACCCCTAAGCCTAAAGGTCCTTCGTTGGACGT10.9803921568627451No Hit
ATGTGAGCTGCCCGCGAAAATAAGACAGAGAAAACTGAACCGAAAGTTCT10.9803921568627451No Hit
AACCTAATGAAATGAACGCAATTAAAAATCTTTAGTCACATATATTTTAT10.9803921568627451No Hit
AAACAATAGATCATAACCCAAATATTTGATCAAAATTCAAAAGTAATGAT10.9803921568627451No Hit
GTGGAGCTCCATCCTCCTCTGAACAGCCTAAGCCTGTGGTGGTGACCACC10.9803921568627451No Hit
AGTATAATGAGACTTAAATTAGGGATTTGTGTATTCTCGAATAAGATCTT10.9803921568627451No Hit
GCTTCTTTCGTCGGAGGGCTGTCTTTGGGCTTTCCGAAAAGGTATCACAT10.9803921568627451No Hit
ATAAGCATCGAGCTCAATTCGAACAGCACCTTTCTCAGAGACGATGCTTA10.9803921568627451No Hit
ATAAATTGCTCGAACCGGTTTGATTAAACCGGAAAAAATTATCAACCTTG10.9803921568627451No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)