FastQCFastQC Report
Sat 29 Feb 2020
HCMM3BGXF_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMM3BGXF_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23332343
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12620465.408998144764116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTT36350.010.5720761
GCCGTAT22700.010.35177360-61
CGCCGTA23350.09.96151758-59
CGGGAAT19650.09.6547191
CCGTATC23900.09.43476760-61
CGTATCA22800.09.26544362-63
CTGACAT68300.09.0452511
AAGGGGG130300.09.03495382-83
CGGGGAT36700.08.7481341
ATTGATT69400.08.6144116
GACATTG70900.08.5352953
CCGTATA8650.08.50650260-61
ACGCACT38800.08.438639
AAAAGGG136000.08.37736380-81
GGCGGAT36100.08.3545311
ACATTGA86050.08.14359954
CGGCACT27700.08.0782191
CCGGGAT28450.08.0362451
GATCGAG34050.07.947965
AAGCACG45300.07.7558865
Produced by FastQC (version 0.11.5)