FastQCFastQC Report
Sat 29 Feb 2020
HCMM3BGXF_n02_AHy24_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMM3BGXF_n02_AHy24_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24469267
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT7950.013.147231
CGAATCG12200.011.2838493
CGGTAAT22300.010.6523121
CGCGGGT43150.010.2395511
AAGGGGG242700.010.14779382-83
GCGGGTT41950.09.5131871
CGGGTAT22100.09.0289191
GCGGGAT49200.08.9804191
CGGGGTT80050.08.96178151
AGGGGGG328400.08.95309184-85
GCGGAAT36650.08.8148241
CGGGGAT85850.08.6883651
AAACGAC45050.08.5359345
CGGGAAT46850.08.4168041
AACGACT45700.08.4150426
TAGGGGG100800.08.41237550-51
GCGGTAT23750.08.4016471
CGACGAT21700.08.3196031
GTTCGAT24000.08.3141291
TAAACGA40100.08.2869044
Produced by FastQC (version 0.11.5)