FastQCFastQC Report
Sat 29 Feb 2020
HCMM3BGXF_n02_AHy24_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMM3BGXF_n02_AHy24_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16769275
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAAT33650.011.153731
ATTCGTA32300.010.5826225
TATTCGT30050.010.4266464
TTCGTAT33100.010.1838156
CGCGGTT17450.010.0735931
CGCACTT32100.09.7682561
CGGGGTT54500.09.4146751
AGGGGGG226150.09.30485282-83
TCGTATT37600.09.0918997
CGGGGAT66150.09.049381
TCGTGTT37500.08.80391794-95
CCGGGAT49300.08.7694551
CGGTAAT12603.092282E-118.6723221
TTATTCG37300.08.65413
CGTATCA18800.08.46470762-63
CGCGGGT32450.08.3452291
GGGGAAT79600.08.2365271
GCGGGTT29550.08.1995651
CGGGCAT64750.08.0710681
GATCGAG56650.07.9613555
Produced by FastQC (version 0.11.5)