FastQCFastQC Report
Sat 29 Feb 2020
HCMM3BGXF_n02_AHy12_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMM3BGXF_n02_AHy12_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25825518
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA46100.019.11441262-63
GCCGTAT49200.018.39269460-61
CGCCGTA55650.016.47376658-59
CCGTATC60950.014.53518660-61
TCGCCGT68800.013.42863758-59
AAGGGGG345100.013.03466682-83
TGGTCGC74350.012.55292454-55
AAAAGGG393200.011.48878380-81
TAGGGGG136950.011.08275850-51
GCGGGTT47250.010.6588521
TAAAAAA878550.010.608670-71
CGCGTAT6152.0879324E-610.0432581
AGGGGGG541650.010.03644984-85
AAAGGGG357850.09.68320682-83
CGGGGAT87750.09.5836841
CGGGGTT81500.09.5607641
AAAAAAG800950.09.53956678-79
GTCGCCG99300.09.42313656-57
GTATCAT98100.09.27294662-63
CGCGGGT46250.09.2456431
Produced by FastQC (version 0.11.5)