FastQCFastQC Report
Sat 29 Feb 2020
HCMM3BGXF_n02_AHy12_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMM3BGXF_n02_AHy12_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23036229
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGT38350.012.3887111
CGCGAAT12450.010.3035261
CGCGATT7251.6156446E-79.8298011
AAGGGGG243550.09.81003182-83
CGGGGTT69000.09.5709961
TACCGTA18900.09.5475859
AGGGGGG326000.09.27389984-85
CTCGGGT51750.09.2726211
GCGGTAT22100.08.8142031
GCCGTAT28700.08.77227760-61
CGGGGAT78400.08.7264571
TAGGGGG94200.08.59837550-51
CGGGTAT21600.08.57832051
CGTATCA27750.08.5591362-63
CGCGGTT22500.08.4463481
CTCGGAT64000.08.3886091
GCGGGTT34000.08.3842421
CGCGCAT13802.5465852E-118.2627321
CGGGAAT51350.08.2345711
AAAGGGG264300.08.21314782-83
Produced by FastQC (version 0.11.5)