FastQCFastQC Report
Sat 29 Feb 2020
HCMM3BGXF_n02_AHy06_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMM3BGXF_n02_AHy06_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24200950
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAT83250.011.18543151
AAAGGGG289450.010.5440980-81
AAGGGGG307100.010.13120682-83
GCGGGTT45550.09.8043851
GGCGAAT32800.09.4150021
TAGGGGG125100.09.36036850-51
CGTATCA24550.09.2874862-63
AGGGGGG496850.09.2400982-83
CGCGGTT28000.08.9928791
CTCGGTT45000.08.8684491
CGGGTAT23600.08.8577131
GGGTTAA75300.08.83184766-67
AAAAGGG309700.08.8270180-81
CGCGGAT36300.08.768981
CGGGAAT54150.08.598221
CGGGGTT77150.08.4365631
GTCGGAT28950.08.3695571
GCGGGAT55400.08.31845951
GGGGGGA499400.08.07908220-21
GCCGGTT52600.08.0386891
Produced by FastQC (version 0.11.5)