FastQCFastQC Report
Sat 29 Feb 2020
HCMM3BGXF_n02_AHy06_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMM3BGXF_n02_AHy06_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20192050
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGT40250.011.4503111
CGGGGAT62900.010.424131
GCGGGTT38650.010.3262151
CGCGTAT4500.00111196989.5025771
GCGGGAT44200.09.3520831
GCGCGAT29600.08.9889251
CCGGGAT55400.08.9193861
GGCGAAT28250.08.9139221
GTCGGTT41150.08.8906251
CGCGAAT12905.456968E-128.8396061
TACCGTA22700.08.7864229
GCGGAAT37400.08.7657461
GGCAAAT152400.08.479991
GGCGGAT88200.08.4575091
ACGGTTA17450.08.43366053
GCGGTAT20300.08.425931
CGGGAAT50300.08.3123931
CGGCGAT39450.08.1898011
CTACCGT28450.08.17863758
CGCGGAT30050.08.0637511
Produced by FastQC (version 0.11.5)