FastQCFastQC Report
Sat 29 Feb 2020
HCMM3BGXF_n02_AHv24_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMM3BGXF_n02_AHv24_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28770932
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCTGCTT1021690.35511188862425447No Hit
GAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTAC952490.3310598349751061No Hit
ATTTTTATTATCTCTTCGGCAGAAAGCCCCTCAATTATTATGTGGTCATT943060.32778222130586526No Hit
GCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCTGCTTT920190.31983322611863946No Hit
CTGACATTGATTACAATTTGCTTAAATGCATAGTAAGAAATACAGTAAAA906830.31518965044302355No Hit
CTCAATTATTATGTGGTCATTCAATACCTCAGTTCTGCTTTGCCTTCTCC889560.3091870642216248No Hit
TTTTATTATCTCTTCGGCAGAAAGCCCCTCAATTATTATGTGGTCATTCA881080.30623964493051525No Hit
CACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCTGC843500.2931778504776974No Hit
CTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCTGCTTTCAT784500.27267104173059115No Hit
GCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCC780050.2711243417488179No Hit
TTACAATTTGCTTAAATGCATAGTAAGAAATACAGTAAAATTGAATTTAA703190.24440987869284178No Hit
CAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCT701860.24394760656345787No Hit
CAAAACTGTTTCACCCATTTTTATTATCTCTTCGGCAGAAAGCCCCTCAA618710.2150469091512225No Hit
CTCCAAAACTGTTTCACCCATTTTTATTATCTCTTCGGCAGAAAGCCCCT561180.1950510327576458No Hit
TTTTTATTATCTCTTCGGCAGAAAGCCCCTCAATTATTATGTGGTCATTC531570.18475939535083535No Hit
CCCATTTTTATTATCTCTTCGGCAGAAAGCCCCTCAATTATTATGTGGTC511900.17792263385836787No Hit
CAATTGCCTACCTGCTTTCATTGACAGAAGATGGAGAAGGCAAAGCAGAA488590.16982070653811285No Hit
CACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCTGCTTTC342700.11911327724802243No Hit
TTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCTGCTTTCATTGACA341840.11881436444255611No Hit
GTCATTCAATACCTCAGTTCTGCTTTGCCTTCTCCATCTTCTGTCAATGA332400.1155332750430191No Hit
ATTTGCTTAAATGCATAGTAAGAAATACAGTAAAATTGAATTTAATGCAA319760.11113995194872381No Hit
GGAGAAGGCAAAGCAGAACTGAGGTATTGAATGACCACATAATAATTGAG319370.11100439846717512No Hit
CCAAAACTGTTTCACCCATTTTTATTATCTCTTCGGCAGAAAGCCCCTCA301400.10475851112504801No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATTAC359950.028.7325948
CTGACAT344200.028.5307431
TTGATTA369450.028.3644357
ATTGATT384700.027.8923476
GACATTG358900.027.313743
CATTGAT420800.025.2729075
ACATTGA404750.024.784134
TGACATT393400.024.5729082
AAGCACG426200.024.1411842
AGCACGC444350.023.3752773
GCACGCA463450.022.6079124
CGCAGAA76250.022.430871
GATTACA511700.020.1664379
CGTATCA40000.019.89199362-63
GCCGTAT41700.019.65018560-61
GAAGCAC539050.019.3723431
ACGCACT579100.018.725846
CACGCAC564900.018.4562595
CGCCGTA47500.017.75024858-59
CAGAAGC539400.017.4552042
Produced by FastQC (version 0.11.5)