FastQCFastQC Report
Sat 29 Feb 2020
HCMM3BGXF_n02_AHv24_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMM3BGXF_n02_AHv24_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27447341
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAATTATTATGTGGTCATTCAATACCTCAGTTCTGCTTTGCCTTCTCC625940.22805123454399465No Hit
CGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCTGCTT569200.2073789224245802No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG561830.2046937807199612No Hit
CTGACATTGATTACAATTTGCTTAAATGCATAGTAAGAAATACAGTAAAA542370.19760384075091283No Hit
ATTTTTATTATCTCTTCGGCAGAAAGCCCCTCAATTATTATGTGGTCATT484330.17645789440951676No Hit
CACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCTGC479410.17466537104632468No Hit
GCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCTGCTTT472090.17199844604255107No Hit
GAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTAC447260.1629520324027016No Hit
CTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCTGCTTTCAT435470.15865653434334495No Hit
CTCCAAAACTGTTTCACCCATTTTTATTATCTCTTCGGCAGAAAGCCCCT408530.14884137592781754No Hit
TTTTATTATCTCTTCGGCAGAAAGCCCCTCAATTATTATGTGGTCATTCA393750.14345651915790314No Hit
GCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCC383380.13967837540255723No Hit
TTACAATTTGCTTAAATGCATAGTAAGAAATACAGTAAAATTGAATTTAA379910.13841413636388314No Hit
CAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCT374920.1365961096195074No Hit
CAATTGCCTACCTGCTTTCATTGACAGAAGATGGAGAAGGCAAAGCAGAA365800.13327338338529768No Hit
CAAAACTGTTTCACCCATTTTTATTATCTCTTCGGCAGAAAGCCCCTCAA350860.12783023317267783No Hit
CCCATTTTTATTATCTCTTCGGCAGAAAGCCCCTCAATTATTATGTGGTC301740.10993414626210968No Hit
GTCATTCAATACCTCAGTTCTGCTTTGCCTTCTCCATCTTCTGTCAATGA281990.10273854942815773No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGATT252450.027.9288166
TTGATTA251100.027.4191747
CTGACAT246200.026.3209061
TGATTAC264650.025.8018078
GACATTG276650.024.674893
CGTATCA50900.024.17151562-63
CATTGAT315200.022.2471895
TGACATT282850.022.225252
ACATTGA326150.021.5428834
GCCGTAT60450.020.19528460-61
GATTACA339050.020.1410089
CGCCGTA59900.019.626758-59
CCGTATC59150.019.35421260-61
AAGCACG269950.019.0484662
AGCACGC282650.018.5080073
GCACGCA290600.018.3460944
ACGCACT338100.016.7958092
CACGCAC346100.016.595841
CGCACTT417350.016.380791
CGCAGAA79250.016.0061361
Produced by FastQC (version 0.11.5)