FastQCFastQC Report
Sat 29 Feb 2020
HCMM3BGXF_n02_AHv12_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMM3BGXF_n02_AHv12_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25767371
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG264430.10262203311311813No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA40500.016.47947362-63
AAGCACG130450.015.6501142
GCCGTAT44000.015.6002760-61
AGCACGC143050.014.9002173
GCACGCA147950.014.7603114
ACGCACT144500.014.654196
CGCCGTA47850.013.89807558-59
CGCACTT158200.013.5063217
CCGTATC51900.013.3171960-61
TGATTAC143700.013.2179148
ATTGATT178350.013.2039356
CACGCAC166300.012.6755795
TTGATTA157600.012.5635077
AAGGGGG322200.012.30275182-83
GACATTG203950.012.1734063
CTGACAT174900.011.9248481
GCGGGTT40650.011.6873911
GCGAGTT79300.011.5028691
TCGCCGT60300.010.98918358-59
GCGGTCT67400.010.9257161
Produced by FastQC (version 0.11.5)