FastQCFastQC Report
Sat 29 Feb 2020
HCMM3BGXF_n02_AHv12_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMM3BGXF_n02_AHv12_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21799139
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTT150750.017.5551761
AAGCACG111650.014.6717742
TGATTAC148400.014.20702658
ACGCACT128000.014.1323486
ATTGATT180200.013.9906866
AGCACGC118600.013.9293263
GCACGCA121900.013.9034624
TTGATTA160150.013.786577
CGCGTAA3701.2862745E-512.8411991
CTGACAT170000.012.6886161
CACGCAC137900.011.9900861
GCGGGTT29550.011.8982081
GACATTG201350.011.5761833
GCGAGTT78900.010.8393391
AATCCGC40700.010.7318555
ACATTGA237250.010.6654574
CGCAGAA53500.010.5681871
CAGAAGC298950.010.3781981
CGGGGTT62950.010.1893231
CGCGATT7051.0736039E-710.109031
Produced by FastQC (version 0.11.5)