FastQCFastQC Report
Sat 29 Feb 2020
HCMM3BGXF_n02_AHv06_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMM3BGXF_n02_AHv06_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17729694
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCGC33450.011.3550425
GCGGGTT29450.010.6488821
CGCGATT6302.493416E-710.5592451
CGTATCA20450.010.33674962-63
GCGGTCT56700.010.0564231
AAATCCG61150.09.9377114
GCGAGTT51150.09.6612441
GCGGAAT28150.09.4526821
CCGGGAT47700.09.3638591
GCGGTAT18300.09.3475281
CGGGGTT51950.09.3295351
CGGTCTT57500.09.248882
GCCGTAT22350.09.24528860-61
GGCGGAT66350.09.2383451
CGGGGAT81250.09.181671
CGCGGTT18200.09.1378091
GGCGAAT38150.08.9677471
CGCACTT34500.08.8146741
CGGACAT33750.08.7289761
CGGGCAT65200.08.599631
Produced by FastQC (version 0.11.5)