FastQCFastQC Report
Sat 29 Feb 2020
HCMM3BGXF_n02_AHv06_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMM3BGXF_n02_AHv06_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23615683
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG298330.12632706832997376No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA43900.017.96212662-63
GCCGTAT53000.015.81906260-61
AAGGGGG397450.014.365355582-83
TAGGGGG152300.013.61512250-51
CGCCGTA60200.013.33492658-59
AAAGGGG409800.012.81422280-81
CCGTATC62750.012.79336860-61
TCGCCGT65950.012.208302558-59
GGGTTAA99550.012.16807466-67
AAAAGGG435050.012.15238380-81
AATCCGC39850.012.1523235
GCGGTCT80300.011.7162231
TGGTCGC72150.011.61928254-55
CGGTCTT76950.011.4771242
GCGGGTT39400.011.3362551
TAATCGA48000.011.2753224
TTTAGGG128050.010.89003448-49
GCGAGTT59650.010.8334411
TAAAAAA943350.010.69559770-71
CGGGGTT63900.010.5590651
Produced by FastQC (version 0.11.5)