FastQCFastQC Report
Sat 29 Feb 2020
HCMM3BGXF_n02_AHmock24_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMM3BGXF_n02_AHmock24_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30611819
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG309610.10114067380314773No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA60700.024.76866762-63
GCCGTAT69000.022.68385560-61
CGCCGTA81300.019.4266158-59
CCGTATC83750.018.46191260-61
TCGCCGT95150.016.1745558-59
TGGTCGC98450.015.51068754-55
AAAAGGG551850.014.63736480-81
AAGGGGG547300.014.56347382-83
TAGGGGG199900.013.64095350-51
GTATCAT116750.013.32514862-63
GTCGCCG127150.012.757256-57
TAAAAAA1276500.012.68243870-71
AAAGGGG509800.012.30373682-83
CGGGGAT105600.011.6079371
AAAAAAG1147100.011.55357278-79
GCGGGTT53350.011.3992111
GTGGTCG142400.011.09040654-55
TTTAGGG174250.011.08413848-49
AGGGGGG873800.010.95888284-85
GGTCGCC143250.010.84261856-57
Produced by FastQC (version 0.11.5)